Beacon Nucleic Acid Ligands of Agar Magnetic Beads Mediated Immune PCR Detection of Hbsag
Science Discovery
Volume 4, Issue 5, October 2016, Pages: 285-291
Received: Oct. 13, 2016; Published: Oct. 13, 2016
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Authors
Li Dongdong, Gansu Province Medical Science Research Institute and College of Life Science of Cell Biology, Lanzhou University, Lanzhou, China
Liao Shiqi, Gansu Province Medical Science Research Institute, Lanzhou, China
Yuan Hongxia, Gansu Province Medical Science Research Institute, Lanzhou, China
Zhao Yunwang, Department of Biochemistry and Molecular Biology, Northwest Normal University, Lanzhou, China
Li Xing, Department of Biochemistry and Molecular Biology, Northwest Normal University, Lanzhou, China
Wang Jing, Department of Biochemistry and Molecular Biology, Northwest Normal University, Lanzhou, China
Wang Jin, Department of Biochemistry and Molecular Biology, Northwest Normal University, Lanzhou, China
Tian Caiping, Gansu Province Medical Science Research Institute, Lanzhou, China
Wei Zhengli, Gansu Province Medical Science Research Institute, Lanzhou, China
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Abstract
Severe hepatitis disease risk, mortality is high in the diagnosis of phenotypic showed the most late for hepatitis, diagnosis and treatment has been late right now, therefore, can find a effective, sensitive, rapidly and accurately identify antigen of second liver surface, the method of early diagnosis and treatment of hepatitis b virus infection has very important significance. In this article, we use mouse IgG Fc fragment nucleic acid beacon as the detection molecules to detecte HBsAg, The 5 'end of the ligand binds a double-stranded beacon sequence as detection molecules, which has specific binding capacity for mouse IgG Fc fragment. A carboxyl group of agarose beads was used as a carrier of combining with hepatitis B goat anti-human polyclonal antibody as a capture molecule to catch hepatitis B virus surface antigen in the liquid to be detected, then combining with mouse anti-human IgG immunoglobulin for HBsAg and nucleic acid beacon to form beads-Polyclonal Antibody-antigen-monoclonal antibody-beacon complexes. Hepatitis B surface antigen quantitative detection technology was achieved by using Real Time-PCR to detect nucleic acid beacon. We termed the detection technology as Immune-PCR detection technology, which is a fast, stable, accurate and sensitive technology for hepatitis B surface antigen detection,In clinic for the early diagnosis and treatment of hepatitis b virus (HBV) infection has very important significance.
Keywords
Carboxyl Agarose Beads, Nucleic Acid Beacon, HBsAg, Hepatitis B Mouse Anti-human IgG, Immune–PCR
To cite this article
Li Dongdong, Liao Shiqi, Yuan Hongxia, Zhao Yunwang, Li Xing, Wang Jing, Wang Jin, Tian Caiping, Wei Zhengli, Beacon Nucleic Acid Ligands of Agar Magnetic Beads Mediated Immune PCR Detection of Hbsag, Science Discovery. Vol. 4, No. 5, 2016, pp. 285-291. doi: 10.11648/j.sd.20160405.14
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