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Method for Improving Rolling Circle Amplification Sensitivity and Specificity

Received: 27 December 2017    Accepted:     Published: 28 December 2017
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Abstract

Rolling circle amplification (RCA) of genomic or plasmid DNA has found wide used in technique academic and biotechnology reseach. In this study, we have successfully found what caused the nonspecific product in the reaction, thus designing an improved RCA which overcomes false priming at sites where primer dimers are formed. The results demonstrated that PEG or PEI could interact with the components of the improved RCA system, and help increase the reaction rate and yield of the improved RCA system, and dramatically reduces the nonspecific RCA products. This low-cost and efficient method dramatically overcomes nonspecific product amplification phenomenon in the RCA reaction which is problematic and has existed for a long time.

DOI 10.11648/j.sd.20170506.28
Published in Science Discovery (Volume 5, Issue 6, November 2017)
Page(s) 496-501
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Rolling Circle Amplification, Recombinant Plasmid, Polyetherimide, Polyethylene Glycol

References
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Author Information
  • School of Biomedicial Sciences, Huaqiao University, Quanzhou, China

  • Key Laboratory of Oral Medicine, Guangzhou Institute of Oral Disease, Stomatology Hospital of Guangzhou Medical University, Guangzhou, China

  • School of Biomedicial Sciences, Huaqiao University, Quanzhou, China

  • Dairy and Functional Foods Research Units, Eastern Regional Research Center, Agriculture Research Service, United States Department of Agriculture, Wyndmoor, The United States of America

  • Dairy and Functional Foods Research Units, Eastern Regional Research Center, Agriculture Research Service, United States Department of Agriculture, Wyndmoor, The United States of America

  • School of Biomedicial Sciences, Huaqiao University, Quanzhou, China

  • School of Biomedicial Sciences, Huaqiao University, Quanzhou, China

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  • APA Style

    Yun Wei Zhang, Dan Hou, Wei Dong Xiao, Jenni Firrman, Lin Shu Liu, et al. (2017). Method for Improving Rolling Circle Amplification Sensitivity and Specificity. Science Discovery, 5(6), 496-501. https://doi.org/10.11648/j.sd.20170506.28

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    ACS Style

    Yun Wei Zhang; Dan Hou; Wei Dong Xiao; Jenni Firrman; Lin Shu Liu, et al. Method for Improving Rolling Circle Amplification Sensitivity and Specificity. Sci. Discov. 2017, 5(6), 496-501. doi: 10.11648/j.sd.20170506.28

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    AMA Style

    Yun Wei Zhang, Dan Hou, Wei Dong Xiao, Jenni Firrman, Lin Shu Liu, et al. Method for Improving Rolling Circle Amplification Sensitivity and Specificity. Sci Discov. 2017;5(6):496-501. doi: 10.11648/j.sd.20170506.28

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  • @article{10.11648/j.sd.20170506.28,
      author = {Yun Wei Zhang and Dan Hou and Wei Dong Xiao and Jenni Firrman and Lin Shu Liu and Yong Diao and Zhi Qing Qi},
      title = {Method for Improving Rolling Circle Amplification Sensitivity and Specificity},
      journal = {Science Discovery},
      volume = {5},
      number = {6},
      pages = {496-501},
      doi = {10.11648/j.sd.20170506.28},
      url = {https://doi.org/10.11648/j.sd.20170506.28},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.sd.20170506.28},
      abstract = {Rolling circle amplification (RCA) of genomic or plasmid DNA has found wide used in technique academic and biotechnology reseach. In this study, we have successfully found what caused the nonspecific product in the reaction, thus designing an improved RCA which overcomes false priming at sites where primer dimers are formed. The results demonstrated that PEG or PEI could interact with the components of the improved RCA system, and help increase the reaction rate and yield of the improved RCA system, and dramatically reduces the nonspecific RCA products. This low-cost and efficient method dramatically overcomes nonspecific product amplification phenomenon in the RCA reaction which is problematic and has existed for a long time.},
     year = {2017}
    }
    

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    T1  - Method for Improving Rolling Circle Amplification Sensitivity and Specificity
    AU  - Yun Wei Zhang
    AU  - Dan Hou
    AU  - Wei Dong Xiao
    AU  - Jenni Firrman
    AU  - Lin Shu Liu
    AU  - Yong Diao
    AU  - Zhi Qing Qi
    Y1  - 2017/12/28
    PY  - 2017
    N1  - https://doi.org/10.11648/j.sd.20170506.28
    DO  - 10.11648/j.sd.20170506.28
    T2  - Science Discovery
    JF  - Science Discovery
    JO  - Science Discovery
    SP  - 496
    EP  - 501
    PB  - Science Publishing Group
    SN  - 2331-0650
    UR  - https://doi.org/10.11648/j.sd.20170506.28
    AB  - Rolling circle amplification (RCA) of genomic or plasmid DNA has found wide used in technique academic and biotechnology reseach. In this study, we have successfully found what caused the nonspecific product in the reaction, thus designing an improved RCA which overcomes false priming at sites where primer dimers are formed. The results demonstrated that PEG or PEI could interact with the components of the improved RCA system, and help increase the reaction rate and yield of the improved RCA system, and dramatically reduces the nonspecific RCA products. This low-cost and efficient method dramatically overcomes nonspecific product amplification phenomenon in the RCA reaction which is problematic and has existed for a long time.
    VL  - 5
    IS  - 6
    ER  - 

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