The Immune-Regulatory Effect of the Exosomes from Human Umbilical Cord Blood Plasma on T Cells in vitro
Science Discovery
Volume 6, Issue 1, February 2018, Pages: 35-42
Received: May 1, 2018; Published: May 23, 2018
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Authors
Weiyi Xia, State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, China
Zheng Ye, State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, China
Hailing Li, Zhongda Hospital, Department of Obstetrics & Gynecology,Southeast University, Nanjing, China
Doulathunnisa Jaffar Ali, State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, China
Jiahua Ding, Zhongda Hospital, Department of Hematology, Key Deptment Jiangsu Medicine, Southeast University, Nanjing, China
Bo Sun, State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, China
Zhongdang Xiao, State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, China
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Abstract
To investigate the immune-regulatory function of exosomes from human umbilical cord blood plasma on T cells in peripheral blood, differential centrifugation was used to isolate the exosomes from umbilical cord blood plasma (Pexo) firstly. The exosome was characterized by transmission electronic microscope and the western blot of CD63, Lamp2. Exosomes were cultured with peripheral blood lymphocytes which were isolated from the peripheral blood for 72 h, and analyzed for the changes of the Treg subpopulation and memory T cells by flow cytometry, and their effect on lymphocyte proliferation was measured by CFSE assay. The production of IFN-γ, IL-2, TNF-α, TGF-β was detected by qRT-PCR. The results show that Pexo could not activate T cells or proliferate the T cells in vitro, and could not regulate the Treg subpopulation or memory T cells, but the expression of IFN-γ and TNF-α was decreased significantly after the treatment with Pexo within 24h. The exosomes from the umbilical cord blood plasma can decrease the expression of inflammatory factors observably under the condition of keeping the activity and function of the cells. So it has the potential to be used to cure the related immunological diseases due to the inflammatory factors overexpression, especially for the elderly, as their body are usually under the inflammatory state.
Keywords
Human Umbilical Cord Blood Plasma, Immune, Exosome, Treg Cells
To cite this article
Weiyi Xia, Zheng Ye, Hailing Li, Doulathunnisa Jaffar Ali, Jiahua Ding, Bo Sun, Zhongdang Xiao, The Immune-Regulatory Effect of the Exosomes from Human Umbilical Cord Blood Plasma on T Cells in vitro, Science Discovery. Vol. 6, No. 1, 2018, pp. 35-42. doi: 10.11648/j.sd.20180601.16
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