Isolation of Pseudomonas fluorescens Species from Rhizospheric Soil of Healthy Faba Bean and Assessed Their Antagonistic Activity Against Botrytis fabae (Chocolate Spot Diseases)
International Journal of Science, Technology and Society
Volume 4, Issue 2, March 2016, Pages: 25-34
Received: Jun. 11, 2015; Accepted: Jun. 19, 2015; Published: Mar. 19, 2016
Views 4990      Downloads 146
Author
Fekadu Alemu, Department of Biology, College of Natural and Computational Sciences, Dilla University, Dilla, Ethiopia
Article Tools
Follow on us
Abstract
Crop protection is an important area of agriculture which needs attention because of most of the hazardous inputs added into the agricultural system are in the form of chemicals. Production of the crop is, however, constrained by several disease infections including fungal diseases. The present study, was isolate twelve Pseudomonas fluorescens isolates from rhizospheric soil of faba bean and were tested for their antagonistic activity against Botrytis fabae that is known to attack faba bean crops. All Pseudomonas fluorescens isolates are shown successfully employed in controlling chocolate spot diseases of plant. Pseudomonas fluorescens isolates 10 (88.1%) showed highest antagonistic activity against Botrytis fabae. All isolate of Pseudomonas fluorescens are indicated successfully employed in controlling chocolate spot diseases of plant due to their antifungal metabolites. Therefore, these isolates can be used as potential of biocontrol agents.
Keywords
Biocontrol, Botrytis fabae, Chocolate Spot Diseases, Faba Bean, Pseudomonas fluorescens
To cite this article
Fekadu Alemu, Isolation of Pseudomonas fluorescens Species from Rhizospheric Soil of Healthy Faba Bean and Assessed Their Antagonistic Activity Against Botrytis fabae (Chocolate Spot Diseases), International Journal of Science, Technology and Society. Vol. 4, No. 2, 2016, pp. 25-34. doi: 10.11648/j.ijsts.20160402.12
References
[1]
Abdelmonem, A. M., 1981. Studies on chocolate spot of broad bean in Libya. Annu. Agric. Sci., 16: 119-131.
[2]
Anitha, A., Das, M. A., 2011. Activation of rice plant growth against Rhizoctonia solani using Pseudomonas fluorescens, Trichoderma and salicylic acid. Res. Biotechnol., 2: 07-12.
[3]
Asfaw T., Beyene, D., Tesfaye, G., 1994. Genetic and Breeding of Field Pea. First Natinal Cool season Food Legumes Conference. ICARDA, Aleppo.
[4]
Asfaw, T., 1979. Broad beans (Vicia faba) and dry peas (Pisum sativum) in Ethiopia. In: Food Legume Improvement and Development, pp. 80-82, (Hawtin, G. C. and Chancellor, G. J. eds). International Development Research Centre, Aleppo.
[5]
Baker, K. F., 1977. Evolving concepts of biological control of plant pathogens. Annu. Rev. Phytopathol., 125: 67-85.
[6]
Bernier, C. C., Hanounik, S. B., Hussein, M. M., Mohamed, H. A., 1993. Field manual of common faba bean diseases in the Nile Valley. International Center for Agricultural Research in the Dry Areas (ICARDA), Aleppo.
[7]
Chand, T., Logan, C., 1984. Antagonists and parasites of Rhizoctonia solani and their efficacy in reducing stem canker of potato under controlled conditions. Transanctions British Mycol. Soc., 38: 107-112.
[8]
Conner, L. L., 1967. An annotated index of plant diseases in Canada. Can. Dept. of Agric. Publication 1251.
[9]
Couillerot, O., Prigent-Combaret, C., Caballero-Mellado, J., Moe¨nne-Loccoz, Y., 2009. Pseudomonas fluorescens and closely-related fluorescent pseudomonads as biocontrol agents of soil-borne phytopathogens. Lett. Appl. Microbiol., 48: 505–512.
[10]
Dahiya, J. S., Woods, D. L., Tewari, J. P., 1988. Control of Rhizoctonia solani, causal agent of brown girdling root rot of rapeseed by Pseudomonas fluorescens. Botanical Bulletin of Academic Sinica, 29: 135-141.
[11]
Deverall, B. L., Wood, R. K. S., 1961. Infection of bean plants (Vicia faba L.) with Botrytis cinerea and Botrytis fabae. Annu. Appl. Biol., 49: 461-472.
[12]
El-Fallal, A. A., Migahed, F. F., 2003. Metabolic change in broad bean infected by Botrytis fabae in response to mushroom spent straw. Asian J. Plant Sci., 2: 1059-1068.
[13]
Gaunt, R. E. 1983. Shoot diseases caused by fungal pathogens. In: The Faba Bean (Vicia faba L.), pp. 463-492, (Hebblethwaite, P. D., ed). Butterworths, London.
[14]
Griffiths, E., Amin, S. M., 1977. Effects of Botrytis fabae infection and mechanical defoliation on seed yield of field beans (Vicia faba). Annu. Appl. Biol., 86: 359-367.
[15]
Harrison, J. G., 1984. Effect of humidity on infection of field bean leaves by Botrytis fabae and germination of conidia. Trans. Br. Mycol. Soc., 82: 245-248.
[16]
Harrison, J. G., 1988. The biology of Botrytis spp. on Vicia beans and chocolate spot disease a review. Plant Pathol., 37: 168–201.
[17]
Harrison, J. G., 1980. Effects of environmental factors on growth of lesions on field beans by Botrytis fabae. Annu. Appl. Biol., 95: 53-61.
[18]
Hebblethwaite, P. D., 1983. The Faba Bean. Butter worths, London.
[19]
Howell, C. R., Stipanovic, R. D., 1979. Control of Rhizoctonia solani on cotton seedlings with Pseudomonas fluorescens and with an antibiotic produced by the bacterium. Phytopathol., 69: 480-482.
[20]
ICARDA, 2006. Technology Generations and Dissemination for Sustainable Production of Cereals and Cool Season Legumes. International Center for Agricultural Research in the Dry Areas, Aleppo.
[21]
Ikata, S., 1933. Studies on red spot disease of Vicia faba. Rep. Agric. Expt. Sta. Okayamaken.
[22]
Ingram, J., Hebblethwaite, P. D., 1976. Optimum economic seed rates in spring and autumn sown field beans. Agric. Prog., 51: 1-32.
[23]
Khaled, A. A., Abd El-Moity, S. M. H., Omar, S. A. M., 1995. Chemical control of some faba bean diseases with fungicides. Egypt J. Agric. Res., 73: 45-56.
[24]
Khalil, S. A., Erskine, W., 2001. Combating disease problems of grain legumes in Egypt. Rain legumes no. 32 – 2nd quarter.
[25]
Khan, M. S., Zaidi, A., 2002. Plant growth promoting rhizobacteria from rhizosphere of wheat and chikpea. Annu. Plant. Protec. Sci., 10: 265-271.
[26]
King, E. O., Ward, M. K., Raney, D. E., 1954. Two simple media for the demonstration of pyocyanine and fluorescein. J. Lab. Clin. Med., 44: 301-307.
[27]
Mahmoud, Y. A. G., Ebrahim, M. K. H., Aly, M. M., 2004. Influence of plant extracts and microbioagants on physiological traits of faba bean infected with Botrytis fabae. J. Plant Biol., 47: 194-202.
[28]
Mansfield, J. W., Deverall, B. J., 1974. Changes in wyerone acid concentrations in leaves of Vicia faba after infection by Botrytis cinerea or B. fabae. Annals Appl. Biol., 77: 227-235.
[29]
Mengistu, A., 1979. Food legume diseases in Ethiopia. In: Food Legume Improvement and Development, pp.106-108, (Hawtin, G. C. and Chancellor, G. J., eds). ICARDA/IDRC, Ottawa.
[30]
Montealegre, J. R., Perez, L. M., Herrera, R., Silva, P., Besoain, X., 2003. Selection of bioantagonisic bacteria to be used in biological control of Rhizoctonia solani in tomato. Environ Biotechnol., 6: 1-9.
[31]
Moore, K. G., Leach, R., 1968. The effect of 6-benzyl- ami-nopurine (benzyladenine) on senescence and chocolate spot (Botrytis fabae) of winter beans (Vicia faba). Annu. Appl. Biol., 61: 55-76.
[32]
Mushtaq, S., Ali, A., Khokhar, I., Mukhtar, I., 2010. Antagonisitic potential of soil bacteria against food borne fungi. World Appl. Sci. J., 11: 966-969.
[33]
Noorka, I. R. El-Bramawy, M. A. S., 2011. Inheritance assessment of chocolate spot and rust disease tolerance in mature faba bean (Vicia faba L.) plants. Pak. J. Bot., 43: 1389-1402.
[34]
Papavizas, G. C., 1985. Trichoderma and Gliocladium: biology, ecology and potential biocontrol. Annu. Rev. Phytopathol., 23: 23-54.
[35]
Paullitz, T. C., Bekanger, R. R., 2000. Biological control in greenhouse systems. Annu. Rev. Phytopathol., 39: 103-133.
[36]
Ramyasmruthi, S., Pallavi, O., Pallavi, S., Tilak, K., Srividya, S., 2012. Chitinolytic and secondary metabolite producing Pseudomonas fluorescens isolated from Solanaceae rhizosphere effective against broad spectrum fungal phytopathogens. Asian J. Plant Sci. Res., 2: 16-24.
[37]
Rangeshwaran, R., Prasad, R. D., 2000. Biological controls of Sclerotium rot of sunflower. Indian Phytopathol., 53: 444-449.
[38]
Reddy, B. P., Rani, J., Reddy, M. S., Kumar, K. V. K., 2010. Isolation of siderophore- producing strains of rhizobacterial fluorescent Pseudomonads and their biocontrol against rice fungal pathogens. Int. J. Appl. Biol. Pharm. Technol. 1: 133-137.
[39]
Reddy, K. R. N., Choudary, K. A., Reddy, M. S., 2007. Antifungal metabolites of Pseudomonas fluorescens isolated from rhizosphere of rice crop. J. Mycol. Plant Pathol. 37(2).
[40]
Riungu, G. M., Muthorni, J. W., Narla, R. D., Wagacha, J. M., Gathumbi, J. K., 2008. Management of Fusarium head blight of wheat and deoxynivalenol accumulation using antagonistic microorganisms. Plant Pathol. J., 7: 13-19.
[41]
Rosales, A. M., Thomashow, L., Cook, R. J., Mew, T.W., 1995. Isolation and identification of antifungal metabolites produced by rice associated antagonistic Pseudomonas spp. Phytopathol., 85: 1029-1032.
[42]
Sahile, S., Fininsa, C., Sakhuja, P. K., Ahmed, S. 2009. Evaluation of pathogenic isolates in Ethiopia for the control of chocolate spot in faba bean. Afri. Crop Sci. J., 17: 187-197.
[43]
Sahile, S., Fininsa, C., Sakhuja, P. K., Seid, A., 2008. Effect of mixed cropping and fungicides on chocolate spot (Botrytis fabae) of faba bean (Vicia faba) in Ethiopia. Crop Protection, 27:275-282.
[44]
Sakthivel, N., Sivamani, E., Unnamalai, N., Gnanamanickam, S.S., 1986. Plant growth promoting rhizobacteria in enhancing plant growth and suppressing plant pathogens. Curr. Sci., 55: 22-25.
[45]
Sardina, J. R., 1930. A new species of Botrytis attacking broad beans. Rev. Appl. Mycol., 9: 424.
[46]
Sardina, J. R., 1932. Two new diseases of broad beans. Rev. Appl. Mycol., 11: 346-347.
[47]
Sardiña, J. R., 1929. Una nueva especie de Botrytis que ataca a las habas. Real Sociedad Española de Historia Natural Memorias, 15: 291-295.
[48]
Sherga, B. M., 1997. Bacilus isolates as potential biocontrol agents against chocolate spot on Faba beans. Can. J. Microbio. 43: 915-924.
[49]
Siddiqui, I. A., Shaukat, S. S., 2002. Resistance against the damping-off fungus Rhizoctonia solani systemically induced by the plant growth promoting rhizobacteria Pseudomonas aeruginosa (IE-6S+) and Pseudomonas fluorescens (CHA0). J. Phytopathol., 150: 500-506.
[50]
Sivamani, E., Gnanamanickam, S. S., 1988. Biological control of Fusarium. oxysporum in banana by inoculation with Pseudomonas fluorescens. Plant Soil, 107: 3-9.
[51]
Sivan, A., 1987. Biological control of Fusarium crown rot of tomato by Trichoderma harzianum under field conditions. Plant Dis.71: 587-592.
[52]
Temple, T. N., Stockwell, V. O., Zoper, J. E., Johnson, K. B., 2004. Bioavailability of iron to Pseudomonas fluorescens strain A506 on flowers of Pear and apple. Phytopathol., 94: 1286-1294.
[53]
Tesfaye Alemu and Kapoor, I. J., 2004. In Vitro evaluation of Trichoderma and Gliocladium spp against Botrytis corm rot (Botrytis gladiolorum) of Gladiolus. Pest Mgt. J. Ethiopia, 8: 97-103.
[54]
Torres, A. M., Roman, B., Avila, C. M., Satovic, Z., Rubiales, D., Sillero, J. C., Cubero, J.I., Moreno, M. T., 2006. Faba bean breeding for resistance against biotic stresses: towards application of marker technology. Euphytica, 147: 67-80.
[55]
Tupenevich, S. M., Kotova, V. V., 1970. Biological features of the causal agent of bean brown patch. Trudy veses. Inst. Zashch. Rast., 29: 143-150.
[56]
United States Department of Agriculture, 1960. Index of plant diseases in the United States. Agric. Handbook, 165: 275-276.
[57]
Vidhyasekaran, P., Muthamilan, M., 1995. Development of formulations of Pseudomonas fluorescens for control of chickpea wilt. Plant Dis., 79: 782-786.
[58]
Williams, P. F., 1978. Growth of broad beans infected by Botytis fabae. J. Hort. Sci., 50: 415-424.
[59]
Zhang, B., Ramonell, K., Somerville, S., Stacey, G., 1994. Characterization of early, chitin induced gene expression in Arabidopsis. M. P. M. I., 15: 963–970.
ADDRESS
Science Publishing Group
1 Rockefeller Plaza,
10th and 11th Floors,
New York, NY 10020
U.S.A.
Tel: (001)347-983-5186