Determination of the needed amount of liquid sterile Lactamator™ to be mixed to cephalexin, β-lactam antibiotic, for optimum deactivation of its molecule's antibacterial properties was conducted using RP-HPLC method. RP-HPLC method was validated for the parameters as linearity, accuracy, LOD, LOQ, and precision. Before the routine microbiological examination for any pharmaceutical dosage form containing β-lactam antibiotic, it is a must to make inactivate of β-lactam active pharmaceutical ingredient (API) by mixing with beta-lactamase before testing. So, the study indicated that mixing of 0.5 ml liquid sterile Lactamator™ with phosphate buffer solution pH (7.2) containing 50 mg cephalexin, with holding the test sample for 90 minutes prior to HPLC measurement will deactivation of cefalexin molecule's antibacterial properties.
| Published in | World Journal of Applied Chemistry (Volume 2, Issue 4) |
| DOI | 10.11648/j.wjac.20170204.11 |
| Page(s) | 120-128 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
Cephalexin, Lactamator, Keflex, Method Validation
| [1] | Holten, K. B., Onusko, E. M. (2000) Appropriate prescribing of oral beta-lactam antibiotics, American Family Physician, 62(3): 611–620. |
| [2] | Drawz, S. M., Bonomo, R. A. (2010) Three decades of β-lactamase inhibitors, Clinical Microbiology Reviews, 23(1): 160–201. |
| [3] | Bush, K., Jacoby, G. A., Medeiros, A. A. (1995) A functional classification scheme for beta-lactamases and its correlation with molecular structure, Antimicrobial Agents and Chemotherapy, 39(6): 1211–1233. |
| [4] | Ambler, R. P. (1980) The structure of beta-lactamases, Philosophical Transactions of the Royal Society B: Biological Sciences, 289(1036): 321–331. |
| [5] | Bush, K., Jacoby, G. A. (2010) Updated functional classification of beta-lactamases, Antimicrobial Agents and Chemotherapy, 54 (3): 969–976. |
| [6] | Philippon, A., Arlet, G., Jacoby, G. A. (2002) Plasmid-determined AmpC-type beta-lactamases, Antimicrobial Agents and Chemotherapy, 46 (1): 1–11. |
| [7] | Jacoby, G. A., Munoz-Price, L. S. (2005) The new beta-lactamases, The New England Journal of Medicine, 352 (4): 380–391. |
| [8] | Zhu, Y., Englebert, S., Joris, B., Ghuysen, J. M., Kobayashi, T., Lampen, J. O. (1992) Structure, function, and fate of the BlaR signal transducer involved in induction of beta-lactamase in Bacillus licheniformis, Journal of Bacteriology, 174: 6171–6178. |
| [9] | Fuda, C. C., Fisher, J. F., Mobashery, S. (2005) Beta-lactam resistance in Staphylococcus aureus: the adaptive resistance of a plastic genome, Cellular and Molecular Life Sciences, 62: 2617–2633. |
| [10] | Safo, M. K., Zhao, Q., Ko, T. P., Musayev, F. N., Robinson, H., Scarsdale, N. (2005) Crystal structures of the BlaI repressor from Staphylococcus aureus and its complex with DNA: insights into transcriptional regulation of the bla and mec operons Journal of Bacteriology, 187: 1833–1844. |
| [11] | Ambler, R. P., Coulson, A. F. W., Frère, J. M., Ghuysen, J. M., Joris, B., Forsman, M., Levesque, R. C., Tiraby, G., Waley, S. G. (1991) A standard numbering scheme for the class A β-lactamases, Biochemical Journal, 276: 269–272. |
| [12] | Jacobs, C., Frere, J. M., Normark, S. (1997) Cytosolic intermediates for cell wall biosynthesis and degradation control inducible beta-lactam resistance in gram-negative bacteria, Cell, 88: 823–832. |
| [13] | Principles of Pharmacology, 2nd edition, (2011), New Delhi: Paras Medical Publishers. Penicillins, cephalosporins and other beta lactam antibiotics. In: Sharma HL, Sharma KK, editors; pp. 723–724. |
| [14] | Sean, C. (2011) Martindale, The Extra Pharmacopoeia, 37th edition, The Pharmaceutical Press, London, pp. 237–238. |
| [15] | Ronald, T. (2011) The United States Pharmacopeia and The National Formulary. USP 36, NF 31, Supplement 1 Asian Edition, Twinbork parkway, Rockville, pp. 1133–1229. |
| [16] | International Conference on Harmonization, (1995), Draft guideline on validation of analytical procedures: Definitions and Terminology, Federal Register, 60, 11260. |
| [17] | Murray, P. R., Baron, E. J., Pfaller, M. A., Tenover, F. C., Yolken, H. R. (1995) Manual of Clinical Microbiology, 6th edition, ASM Press, Washington DC, pp. 15–18. |
| [18] | Olurinola, P. F. (1996) A laboratory manual of pharmaceutical microbiology, Idu, Abuja, Nigeria, pp. 69–105. |
| [19] | Ronald, T. (2017) The United States Pharmacopeia and The National Formulary. USP 40, NF 35, Supplement 1 Asian Edition, Twinbork parkway, Rockville, pp. 123–130. |
APA Style
Mahmoud Faysal Mshref, Hesham Mohamed Ghonemy, Amr Helmy Ali. (2017). Determination of Beta-Lactamase Inactivation of Cephalexin by Validated RP-HPLC Method. World Journal of Applied Chemistry, 2(4), 120-128. https://doi.org/10.11648/j.wjac.20170204.11
ACS Style
Mahmoud Faysal Mshref; Hesham Mohamed Ghonemy; Amr Helmy Ali. Determination of Beta-Lactamase Inactivation of Cephalexin by Validated RP-HPLC Method. World J. Appl. Chem. 2017, 2(4), 120-128. doi: 10.11648/j.wjac.20170204.11
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Download@article{10.11648/j.wjac.20170204.11,
author = {Mahmoud Faysal Mshref and Hesham Mohamed Ghonemy and Amr Helmy Ali},
title = {Determination of Beta-Lactamase Inactivation of Cephalexin by Validated RP-HPLC Method},
journal = {World Journal of Applied Chemistry},
volume = {2},
number = {4},
pages = {120-128},
doi = {10.11648/j.wjac.20170204.11},
url = {https://doi.org/10.11648/j.wjac.20170204.11},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.wjac.20170204.11},
abstract = {Determination of the needed amount of liquid sterile Lactamator™ to be mixed to cephalexin, β-lactam antibiotic, for optimum deactivation of its molecule's antibacterial properties was conducted using RP-HPLC method. RP-HPLC method was validated for the parameters as linearity, accuracy, LOD, LOQ, and precision. Before the routine microbiological examination for any pharmaceutical dosage form containing β-lactam antibiotic, it is a must to make inactivate of β-lactam active pharmaceutical ingredient (API) by mixing with beta-lactamase before testing. So, the study indicated that mixing of 0.5 ml liquid sterile Lactamator™ with phosphate buffer solution pH (7.2) containing 50 mg cephalexin, with holding the test sample for 90 minutes prior to HPLC measurement will deactivation of cefalexin molecule's antibacterial properties.},
year = {2017}
}
TY - JOUR T1 - Determination of Beta-Lactamase Inactivation of Cephalexin by Validated RP-HPLC Method AU - Mahmoud Faysal Mshref AU - Hesham Mohamed Ghonemy AU - Amr Helmy Ali Y1 - 2017/09/13 PY - 2017 N1 - https://doi.org/10.11648/j.wjac.20170204.11 DO - 10.11648/j.wjac.20170204.11 T2 - World Journal of Applied Chemistry JF - World Journal of Applied Chemistry JO - World Journal of Applied Chemistry SP - 120 EP - 128 PB - Science Publishing Group SN - 2637-5982 UR - https://doi.org/10.11648/j.wjac.20170204.11 AB - Determination of the needed amount of liquid sterile Lactamator™ to be mixed to cephalexin, β-lactam antibiotic, for optimum deactivation of its molecule's antibacterial properties was conducted using RP-HPLC method. RP-HPLC method was validated for the parameters as linearity, accuracy, LOD, LOQ, and precision. Before the routine microbiological examination for any pharmaceutical dosage form containing β-lactam antibiotic, it is a must to make inactivate of β-lactam active pharmaceutical ingredient (API) by mixing with beta-lactamase before testing. So, the study indicated that mixing of 0.5 ml liquid sterile Lactamator™ with phosphate buffer solution pH (7.2) containing 50 mg cephalexin, with holding the test sample for 90 minutes prior to HPLC measurement will deactivation of cefalexin molecule's antibacterial properties. VL - 2 IS - 4 ER -
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