Clonal Structure of Two Wild Lotus (Nelumbo nucifera Gaertn.) Populations Inferred from Amplified Fragment Length Polymorphism Fingerprints

Kazunari Nomura; Tamae Harada; Nozomi Saotome; Minami Kubota; Masato Katori

doi:doi:10.11648/j.plant.20150301.12

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Clonal Structure of Two Wild Lotus (Nelumbo nucifera Gaertn.) Populations Inferred from Amplified Fragment Length Polymorphism Fingerprints

Kazunari Nomura, Tamae Harada, Nozomi Saotome, Minami Kubota, Masato Katori

Published in Plant (Volume 3, Issue 1)

Received: 26 February 2015 Accepted: 13 March 2015 Published: 18 March 2015

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Abstract

The lotus Nelumbo nucifera often forms dense populations at the margins of lakes and ponds. Many aquatic plant species such as N. nucifera are characterized by the ability to reproduce both sexually and asexually, which can affect the genetic diversity of populations growing in different types of environments. We compared the clonal diversities of two natural lotus populations in Japan. Diversity was quantified by DNA fingerprinting of amplified fragment length polymorphisms (AFLPs) in leaves sampled across each population. The DNA fingerprints revealed different amounts of diversity in each population. In 162 samples from Lake Kasumigaura, where lotus has grown continuously for many years, 35 AFLP patterns were detected. One particular clone was distributed widely, suggesting that growth of seed-derived plants was inhibited because of the superior competitive ability of rhizomes, resulting in low genetic diversity within the population. In 214 samples from Uchi-numa Pond in Miyagi Prefecture, where the lotus population was totally destroyed by heavy rains in 1998 and is now recovering, 213 different AFLP profiles were detected. The spatial distance between samples from Uchi-numa Pond was correlated with the genetic distance. These results suggest that, in lotus, sexual reproduction is favored when rapidly varying water level damages the population, because small seedlings are better able to survive in a low-density population. In contrast, vegetative reproduction is favored when environmental conditions are stable.

Published in	Plant (Volume 3, Issue 1)
DOI	10.11648/j.plant.20150301.12
Page(s)	8-14
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Aquatic Plants, Clonal Reproduction, Genotypic Diversity, Nelumbo nucifera

References

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Kazunari Nomura, Tamae Harada, Nozomi Saotome, Minami Kubota, Masato Katori. (2015). Clonal Structure of Two Wild Lotus (Nelumbo nucifera Gaertn.) Populations Inferred from Amplified Fragment Length Polymorphism Fingerprints. Plant, 3(1), 8-14. https://doi.org/10.11648/j.plant.20150301.12

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ACS Style

Kazunari Nomura; Tamae Harada; Nozomi Saotome; Minami Kubota; Masato Katori. Clonal Structure of Two Wild Lotus (Nelumbo nucifera Gaertn.) Populations Inferred from Amplified Fragment Length Polymorphism Fingerprints. Plant. 2015, 3(1), 8-14. doi: 10.11648/j.plant.20150301.12

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AMA Style

Kazunari Nomura, Tamae Harada, Nozomi Saotome, Minami Kubota, Masato Katori. Clonal Structure of Two Wild Lotus (Nelumbo nucifera Gaertn.) Populations Inferred from Amplified Fragment Length Polymorphism Fingerprints. Plant. 2015;3(1):8-14. doi: 10.11648/j.plant.20150301.12

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@article{10.11648/j.plant.20150301.12,
  author = {Kazunari Nomura and Tamae Harada and Nozomi Saotome and Minami Kubota and Masato Katori},
  title = {Clonal Structure of Two Wild Lotus (Nelumbo nucifera Gaertn.) Populations Inferred from Amplified Fragment Length Polymorphism Fingerprints},
  journal = {Plant},
  volume = {3},
  number = {1},
  pages = {8-14},
  doi = {10.11648/j.plant.20150301.12},
  url = {https://doi.org/10.11648/j.plant.20150301.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.plant.20150301.12},
  abstract = {The lotus Nelumbo nucifera often forms dense populations at the margins of lakes and ponds. Many aquatic plant species such as N. nucifera are characterized by the ability to reproduce both sexually and asexually, which can affect the genetic diversity of populations growing in different types of environments. We compared the clonal diversities of two natural lotus populations in Japan. Diversity was quantified by DNA fingerprinting of amplified fragment length polymorphisms (AFLPs) in leaves sampled across each population. The DNA fingerprints revealed different amounts of diversity in each population. In 162 samples from Lake Kasumigaura, where lotus has grown continuously for many years, 35 AFLP patterns were detected. One particular clone was distributed widely, suggesting that growth of seed-derived plants was inhibited because of the superior competitive ability of rhizomes, resulting in low genetic diversity within the population. In 214 samples from Uchi-numa Pond in Miyagi Prefecture, where the lotus population was totally destroyed by heavy rains in 1998 and is now recovering, 213 different AFLP profiles were detected. The spatial distance between samples from Uchi-numa Pond was correlated with the genetic distance. These results suggest that, in lotus, sexual reproduction is favored when rapidly varying water level damages the population, because small seedlings are better able to survive in a low-density population. In contrast, vegetative reproduction is favored when environmental conditions are stable.},
 year = {2015}
}

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TY - JOUR
T1 - Clonal Structure of Two Wild Lotus (Nelumbo nucifera Gaertn.) Populations Inferred from Amplified Fragment Length Polymorphism Fingerprints
AU - Kazunari Nomura
AU - Tamae Harada
AU - Nozomi Saotome
AU - Minami Kubota
AU - Masato Katori
Y1 - 2015/03/18
PY - 2015
N1 - https://doi.org/10.11648/j.plant.20150301.12
DO - 10.11648/j.plant.20150301.12
T2 - Plant
JF - Plant
JO - Plant
SP - 8
EP - 14
PB - Science Publishing Group
SN - 2331-0677
UR - https://doi.org/10.11648/j.plant.20150301.12
AB - The lotus Nelumbo nucifera often forms dense populations at the margins of lakes and ponds. Many aquatic plant species such as N. nucifera are characterized by the ability to reproduce both sexually and asexually, which can affect the genetic diversity of populations growing in different types of environments. We compared the clonal diversities of two natural lotus populations in Japan. Diversity was quantified by DNA fingerprinting of amplified fragment length polymorphisms (AFLPs) in leaves sampled across each population. The DNA fingerprints revealed different amounts of diversity in each population. In 162 samples from Lake Kasumigaura, where lotus has grown continuously for many years, 35 AFLP patterns were detected. One particular clone was distributed widely, suggesting that growth of seed-derived plants was inhibited because of the superior competitive ability of rhizomes, resulting in low genetic diversity within the population. In 214 samples from Uchi-numa Pond in Miyagi Prefecture, where the lotus population was totally destroyed by heavy rains in 1998 and is now recovering, 213 different AFLP profiles were detected. The spatial distance between samples from Uchi-numa Pond was correlated with the genetic distance. These results suggest that, in lotus, sexual reproduction is favored when rapidly varying water level damages the population, because small seedlings are better able to survive in a low-density population. In contrast, vegetative reproduction is favored when environmental conditions are stable.
VL - 3
IS - 1
ER -

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Author Information

Kazunari Nomura

College of Bioresource Sciences, Nihon University, 1866 Kameino Fujisawa, Kanagawa, Japan
Tamae Harada

College of Bioresource Sciences, Nihon University, 1866 Kameino Fujisawa, Kanagawa, Japan
Nozomi Saotome

College of Bioresource Sciences, Nihon University, 1866 Kameino Fujisawa, Kanagawa, Japan
Minami Kubota

College of Bioresource Sciences, Nihon University, 1866 Kameino Fujisawa, Kanagawa, Japan
Masato Katori

Suigo-Sawara Aquatic Botanical Garden, Ogishima, Katori, Chiba 287-0801, Japan

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Kazunari Nomura, Tamae Harada, Nozomi Saotome, Minami Kubota, Masato Katori. (2015). Clonal Structure of Two Wild Lotus (Nelumbo nucifera Gaertn.) Populations Inferred from Amplified Fragment Length Polymorphism Fingerprints. Plant, 3(1), 8-14. https://doi.org/10.11648/j.plant.20150301.12

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ACS Style

Kazunari Nomura; Tamae Harada; Nozomi Saotome; Minami Kubota; Masato Katori. Clonal Structure of Two Wild Lotus (Nelumbo nucifera Gaertn.) Populations Inferred from Amplified Fragment Length Polymorphism Fingerprints. Plant. 2015, 3(1), 8-14. doi: 10.11648/j.plant.20150301.12

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AMA Style

Kazunari Nomura, Tamae Harada, Nozomi Saotome, Minami Kubota, Masato Katori. Clonal Structure of Two Wild Lotus (Nelumbo nucifera Gaertn.) Populations Inferred from Amplified Fragment Length Polymorphism Fingerprints. Plant. 2015;3(1):8-14. doi: 10.11648/j.plant.20150301.12

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@article{10.11648/j.plant.20150301.12,
  author = {Kazunari Nomura and Tamae Harada and Nozomi Saotome and Minami Kubota and Masato Katori},
  title = {Clonal Structure of Two Wild Lotus (Nelumbo nucifera Gaertn.) Populations Inferred from Amplified Fragment Length Polymorphism Fingerprints},
  journal = {Plant},
  volume = {3},
  number = {1},
  pages = {8-14},
  doi = {10.11648/j.plant.20150301.12},
  url = {https://doi.org/10.11648/j.plant.20150301.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.plant.20150301.12},
  abstract = {The lotus Nelumbo nucifera often forms dense populations at the margins of lakes and ponds. Many aquatic plant species such as N. nucifera are characterized by the ability to reproduce both sexually and asexually, which can affect the genetic diversity of populations growing in different types of environments. We compared the clonal diversities of two natural lotus populations in Japan. Diversity was quantified by DNA fingerprinting of amplified fragment length polymorphisms (AFLPs) in leaves sampled across each population. The DNA fingerprints revealed different amounts of diversity in each population. In 162 samples from Lake Kasumigaura, where lotus has grown continuously for many years, 35 AFLP patterns were detected. One particular clone was distributed widely, suggesting that growth of seed-derived plants was inhibited because of the superior competitive ability of rhizomes, resulting in low genetic diversity within the population. In 214 samples from Uchi-numa Pond in Miyagi Prefecture, where the lotus population was totally destroyed by heavy rains in 1998 and is now recovering, 213 different AFLP profiles were detected. The spatial distance between samples from Uchi-numa Pond was correlated with the genetic distance. These results suggest that, in lotus, sexual reproduction is favored when rapidly varying water level damages the population, because small seedlings are better able to survive in a low-density population. In contrast, vegetative reproduction is favored when environmental conditions are stable.},
 year = {2015}
}

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