Physicochemical and Spectroscopic Characterization of Biofield Energy Treated Gerbera Multiplication Medium
Volume 3, Issue 6, November 2015, Pages: 57-63
Received: Oct. 11, 2015; Accepted: Oct. 20, 2015; Published: Nov. 16, 2015
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Mahendra Kumar Trivedi, Trivedi Global Inc., Henderson, USA
Alice Branton, Trivedi Global Inc., Henderson, USA
Dahryn Trivedi, Trivedi Global Inc., Henderson, USA
Gopal Nayak, Trivedi Global Inc., Henderson, USA
Ragini Singh, Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, Madhya Pradesh, India
Snehasis Jana, Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, Madhya Pradesh, India
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The micropropagation technique is used for Gerbera species due to their high demand all over the world as the decorative potted plants and cut flowers. The present study was done to investigate the impact of biofield energy treatment on the physicochemical properties of gerbera multiplication medium. A part of the sample was treated with Mr. Trivedi’s biofield energy, and the other part was kept as untreated and termed as the control sample. Both the parts were subsequently analysed for their physical, thermal and spectral properties using X-ray diffraction (XRD), particle size analysis, surface area analysis, thermogravimetric analysis (TGA), elemental analysis, and Fourier transform infrared (FT-IR) spectroscopy. The XRD results showed 13.98% increase in crystallite size of treated sample (104.01 nm) as compared to the control (91.25 nm). The particle size data revealed an increase in d50 (average particle size) and d99 (size below which 99% particles are present) by 72.57% and 42.26%, respectively of the treated sample as compared to the control. Moreover, the surface area of the treated sample was reduced from 0.694 m2/g (control) to 0.560 m2/g in the treated sample. The TGA data showed the increase in onset temperature along with the reduction in the percent weight loss of the treated sample as compared to the control. Besides, the elemental analysis revealed the significant decrease in the percentage of nitrogen (10.47%) and hydrogen (9.35%) as well as the presence of sulphur in the treated sample. The FT-IR results showed the differences in the IR frequencies corresponding to pyridine ring and N-H2 deformation of the treated sample as compared to the control. Hence, the overall data revealed that the biofield energy treatment had a significant impact on the physicochemical properties of the treated sample that might help to improve its uses in the in vitro tissue culture techniques as compared to the control sample.
Gerbera Multiplication Medium, Biofield Energy Treatment, Micropropagation, Complementary and Alternative Medicines
To cite this article
Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Ragini Singh, Snehasis Jana, Physicochemical and Spectroscopic Characterization of Biofield Energy Treated Gerbera Multiplication Medium, Plant. Vol. 3, No. 6, 2015, pp. 57-63. doi: 10.11648/j.plant.20150306.11
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Sathyanarayana BN (2007) Plant tissue culture: Practices and new experimental protocols. I K International Pvt. Ltd., India.
Prasad MP (2014) In-vitro optimization of growth hormones in the micropropagation of Gerbera species. IJCB 2: 1-5.
Shabanpour K, Sharifi A, Bagheri A, Moshtaghi N (2011) Effect of genotypes and culture medium on shoot regeneration and proliferation of Gerbera jamesonii. Afr J Biotechnol 10: 12211-12217.
Nhut DT, Thuy An TT, Huong NTD, Don NT, Hai NT, et al. (2006) Effect of genotype, explant size, position, and culture medium on shoot generation of Gerbera jamesonii by receptacle transverse thin cell layer culture. Sci Hortic 111: 146-151.
George EF, Hall MA, Klerk GJD (2008) The components of plant tissue culture media I: Macro- and micro-nutrients. Plant propagation by tissue culture, 3rdedn, Springer, Berlin, Heidelberg.
Reynoird JP, Meynet J, Caissard JC, Chriqui D (1997) Micropropagation of gerbera. Biotechnology in agriculture and forestry: High-tech and micropropagation VI. Springer, Berlin, Heidelberg.
Kanwar JK, Kumar S (2008) In vitro propagation of Gerbera- A review. Hortic Sci (Prague), 35: 35-44.
Mager J, Moore D, Bendl D, Wong B, Rachlin K, et al. (2007) Evaluating biofield treatments in a cell culture model of oxidative stress. Explore (NY) 3: 386-390.
Uchida S, Iha T, Yamaoka K, Nitta K, Sugano H (2012) Effect of biofield therapy in the human brain. J Altern Complement Med 18: 875-879.
NIH, National Center for Complementary and Alternative Medicine. CAM Basics. Publication 347. [October 2, 2008].
Saad M, Medeiros RD (2012) Distant healing by the supposed vital energy- scientific bases. Complementary therapies for the contemporary healthcare. InTech.
Prakash S, Chowdhury AR, Gupta A (2015) Monitoring the human health by measuring the biofield "aura": An overview. Int J Appl Eng Res 10: 27654-27658.
Trivedi MK, Nayak G, Patil S, Tallapragada RM, Mishra R (2015) Influence of biofield treatment on physicochemical properties of hydroxyethyl cellulose and hydroxypropyl cellulose. J Mol Pharm Org Process Res 3: 126.
Trivedi MK, Patil S, Shettigar H, Bairwa K, Jana S (2015) Phenotypic and biotypic characterization of Klebsiella oxytoca: An impact of biofield treatment. J Microb Biochem Technol 7: 202-205.
Sances F, Flora E, Patil S, Spence A, Shinde V (2013) Impact of biofield treatment on ginseng and organic blueberry yield. Agrivita J Agric Sci 35: 22-29.
Hartono A, Satira S, Djamal M, Ramli R, Bahar H, et al. (2013) Effect of mechanical treatment temperature on electrical properties and crystallite size of PVDF Film. AMPC 3: 71-76.
Trivedi MK, Nayak G, Tallapragada RM, Patil S, Latiyal O, et al. (2015) Effect of biofield treatment on structural and morphological properties of silicon carbide. J Powder Metall Min 4:132.
Lee S, Song D, Kim D, Lee J, Kim S, et al. (2004) Effects of synthesis temperature on particle size/shape and photoluminescence characteristics of ZnS: Cu nanocrystals. Mater Lett 58: 342-346.
Van der Kamp JW, Jones J, McCleary B, Topping D (2010) Dietary fibre: New frontiers for food and health. Wageningen Academic Publishers, Netherlands.
Norman AG (1968) Advances in Agronomy. Academic Press, New York, USA.
Sovizi MR, Hajimirsadeghia SS, Naderizadehb B (2009) Effect of particle size on thermal decomposition of nitrocellulose. J Hazard Mater 168: 1134-1139.
Lambert JB (1987) Introduction to organic spectroscopy. Macmillan, New York, USA.
Miller FA, Wilkins CH (1952) Infrared spectra and characteristic frequencies of inorganic ions: Their use in qualitative analysis. Analytical Chemistry 24: 1253-1294.
Breda S, Reva ID, Lapinski L, Nowak MJ, Fausto R (2006) Infrared spectra of pyrazine, pyrimidine and pyridazine in solid argon. J Mol Struct 786: 193-206.
Pivovarov VB, Stepanian SG, Reva ID, Sheina GG, Blagoi YP (1995) Infrared spectra and the structure of 1-methyladenine in an argon matrix and solutions. Spectrochim Acta A Mol Biomol Spectrosc 51: 843-853.
Rao CNR, Venkataraghavan R (1964) Contribution to the infrared spectra of five-membered N- and N, S-heterocyclic compounds. Can J Chem 42: 43-49.
Stoyanova A, Iordanova R, Mancheva M, Dimitriev Y (2009) Synthesis and structural characterization of MoO3 phases obtained from molybdic acid by addition of HNO3 and H2O2. J Optoelectron Adv M 11: 1127-1131.
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