Bio-efficacy of Crude Leaf Extracts of Eucalyptus globulus Against In vitro and In vivo Growth of Chocolate Spot (Botrytis fabae Sard.) of Faba Bean (Vicia faba L.)

Addisu Tegegn Tola; Meseret Chimdessa Egigu; Bekele Hundie Egdu

doi:doi:10.11648/j.plant.20160405.12

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Bio-efficacy of Crude Leaf Extracts of Eucalyptus globulus Against In vitro and In vivo Growth of Chocolate Spot (Botrytis fabae Sard.) of Faba Bean (Vicia faba L.)

Addisu Tegegn Tola, Meseret Chimdessa Egigu, Bekele Hundie Egdu

Published in Plant (Volume 4, Issue 5)

Received: 2 September 2016 Accepted: 18 September 2016 Published: 14 October 2016

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Abstract

It is widely known that faba bean (Vicia faba L.) is seriously attacked by the fungal disease, chocolate spot, caused by Botrytis fabae resulting in a yield loss ranging from 50 to 100%. Even though synthetic fungicides are used as one of the effective options for the control of plant diseases, the environmental hazards and economic unfeasibility associated with them necessitate the search for relatively safe natural products. This study was initiated to evaluate the antifungal potential of crude extracts of leaves of Eucalyptus globulus, against in vitro and in vivo growth of Botrytis fabae. In the in vitro experiment, antifungal assay was set up using different concentrations of the crude extracts. In vivo experiment was conducted in the field by planting a faba bean variety, Shallo, (EH011-22-1) and selected plants from each plot were used for extract application and subsequent data collection. Laboratory experiment showed that, compared to the control, extracts obtained from each solvent managed to produce statistically significant (p <0.05) inhibition of mycelial growth. Based on the minimum concentration, inhibition from methanol extract (42.2%) significantly varied from that of ethanol (26.3%). Nevertheless, aqueous extract was nearly statistically the same to that of methanol. On the other hand, at maximum concentration (40%), a maximum inhibition percent of 83.7, which was nearly double of that produced by aqueous extract, was recorded from ethanol extract treated plates. Field experiment has shown that the use of ethanol extract of Eucalyptus globulus produced an efficacy of about 58.4%. Number of flowers aborted, number of tillers per m2, number of pods per plant, number of seeds per pod and hundred seeds weight were affected due to the disease suppression effect of the three extracts. However, there was quantitative variation in yield though no statistically significant difference was observed. The result of this study showed that extracts of the tested plant species have natural fungitoxic potential and showed a promising future for the development of safe natural alternative fungicides used for the control of Botrytis fabae after further pertinent tests and screening of the active principles.

Published in	Plant (Volume 4, Issue 5)
DOI	10.11648/j.plant.20160405.12
Page(s)	37-44
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Botanicals, Crude Extracts, Botrytis fabae, In vitro, In vivo, Vicia faba

References

[1]	Abera, A., Lemessa, F. and Muleta, D., 2011. The antifungal activity of some medicinal plants against coffee berry disease caused by Colletotrichum kahwae. Int. J. of agri. res., 3:268-279.
[2]	Agrios, G. N., 2005. Plant Pathology. 5th ed. Elsevier Inc, ISBN 0-12-044565-4, New York.
[3]	Alabi, D. A., M. Z. Onibudol and N. A. Amusa, 2005b. Chemicals and Nutritional Compositions of four Botanicals with Fungitoxic Properties. World J. Agric., Sci., 1:84-88.
[4]	Asfaw Telaye, Geletu Bejiga, Saxena, Hohan C. and Solh, Mahmoud B. (eds.), 1994. Cool-season Food Legumes of Ethiopia. Proceedings of the First National Cool-season Food Legumes Review Conference, 16-20 December 1993, Addis Ababa, Ethiopia. ICARDA/Institute of Agricultural Research. ICARDA: Aleppo, Syria. Vii + 440 pp.
[5]	Bernier, C. C., S. B. Hanounik, M. M. Hussein and H. A. Mohamed, 1993. Field manual of common faba bean diseases in the Nile Valley. International Center for Agricultural Research in the Dry Areas (ICARDA) Information Bulletin No. 3.
[6]	Branett, H. L., Huter, B. B., 1982. Illustrated genera of imperfect fungi. 3rd ed. Minneapolis, Minnesota: Burgess Pub. Company, p. 241.
[7]	Burt, S., 2004. Essential oils: their antibacterial properties and potential applications in foods a review, Int. J. Food Micribiol., 94: 223-253.
[8]	CSA, 2011. Report on area and production of major crops, (Meher season, Private peasant holdings,): Statistical Bulletin. Central Statistical Authority (CSA), Addis Ababa Ethiopia.
[9]	Deepak, S. A., G. Oros, S. G. Sathyanarayana, H. Shekar Shetty and S. Sashikanth, 2007. Antisporulant Activity of Watery Extracts of Plants against Sclerospora graminicola Causing Downy Mildew Disease of Pearl Millet. American J. of Agri. and Biol. Scie., 2: 36-42.
[10]	Dereje G., 2000. Yield loss of field pea due to Ascochyta blight in central Ethiopia. P. Man. J. Eth., 4: 89-95.
[11]	FAO, 2006. The state of food insecurity in the world. The implementation and midterm review [cited 2007 Dec 2]. Available from: http://www.fao.org/docrep/009/a0750e/a0750e00.HTM - 12k.
[12]	FAOSTAT, www.faoorg, 2004. Available at http://www.rlc.fao.org/progesp/pesa/ caricom/pdf/ Overviewof/ Food and Nutrition Security.
[13]	Farid, A., Md., Khalequzzaman, K. M., Nazrul Islam., Md., Anam, M. K. and Tahasinul Islam, M., 2002. Effect of plant extracts against Bipolaris oryzae of rice under in vitro conditions. Pakistan J. of Biol. Sci., 5: 442-445.
[14]	Haggag, W. M., A. L. Kansoh and A. M. Aly, 2006. Proteases from Talaromyces flavus and Trichoderma harzianum: Purification, characterization and antifungal activity against brown spot disease on faba bean. Plant Pathol. Bull., 15: 231-239.
[15]	ICARDA (International Center for Agricultural Research in the Dry Areas), 2006. Technology Generations and Dissemination for Sustainable Production of Cereals and Cool Season Legumes. Aleppo, Syria. pp256. Institute of Agricultural Research (IAR), 1985. Crop Protection Department Progress Report for the period 1983/84, pages 103-104. IAR, Addis Ababa.
[16]	Isman, M. B., 2006. Botanical insecticides, deterrents, and repellents in modern agriculture and an increasingly regulated world. Annu Rev Entomol., 51: 45–66.
[17]	Joseph, B., Dar, M. A. and Kumar, V., 2008. Bioefficacy of Plant Extracts to Control Fusarium solani F. Sp. Melongenae Incitant of Brinjal Wilt. Global J. Biotech. & Biochem., 3: 56-59.
[18]	Kalemba, D. and A. Kunicka, 2003. Antibacterial and antifungal properties of essential oils. Curr. Med. Chem., 10: 813-829.
[19]	Koike, S. T., 1998. Severe outbreak of chocolate spot of faba bean, caused by Botrytis fabae in California. Plant Disease, 82 (7): 831.
[20]	Koul, O., 2008. Phytochemicals and insect control: an antifeedant approach. Crit. Rev. Plant Sci., 27: 1–24.
[21]	Maggie, E. M. H. Abd El-Rahman, S. El-Abdasi and Mikhail, M. S., 2006. Inducing resistance against faba bean chocolate spot disease. Egypt. J. Phytopathol., 34:69-79.
[22]	Mahmoud, Y. A. G., Souod, A. E., Alsokari, S., Ismaeil, A. E., Attia, M. and Ebrahim, M. K., 2011. Recent approaches for controlling brown spot disease of Faba Bean in Egypt. Egypt. Acad. J. Biolog. Sci., 3: 41-53.
[23]	Mousavi, S. M. and Raftos, D., 2012. In Vitro Antifungal activity of a new combination of Essential oils against some filamentous fungi. Middle-East J. of Scie. Res., 11: 156161.
[24]	Mousavi, S. M., S. S. Mirzargar, H. A. Ebrahim Zadeh Mousavi, R. Omid Baigi, A. Khosravi and M. R. Ahmadi, 2009. Evaluation of antifungal activity of new combined essential oils in comparison with malachite green on hatching rate in Rainbow Trout (Oncorhynchus mykiss) eggs. J. Fish. Aquat. Sci., 4: 103–110.
[25]	Nduagu, C., Ekefan, E. J. and Nwankiti, A. O., 2008. Effect of Some Crude Plant extracts on Growth of Colletotrichum capsici (Synd) Butler and Bisby causal agent of Papper anthracnose. J. App. Bioscie., 6: 184–190.
[26]	Nerio, L. S., Olivero-Verbel, J. and Stashenko, E., 2010. Repellent activity of essential oils: A review. Bioresour Technol., 101: 372–378.
[27]	Pattnaik, M. M, Kar, M. and Sahu, R. K., 2012. Bioefficacy of some plant extracts on growth parameters and control of diseases in Lycopersicum esculentum. Asian J. of Pl. Sci. and Res., 2: 129-142.
[28]	Prince, L. and P. Prabakaran, 2011. Antifungal activity of medicinal plants against plant pathogenic fungus Colletotrichum falcatum. Asian J. of Plant Sci. and res., 1: 84-87.
[29]	Samuel, S., Abang, M. M., Fininsa, C., Ahmed, S., Sakhuja, P. K. and Baum, M., 2012. Pathogenic and genetic diversity of Botrytis fabae Sard. isolates from faba bean fields in different agro-ecological zones of Northern Ethiopia. Archives Of Phytopathol. and Pl. Prot., DOI:10.1080/03235408.2012.664710
[30]	Samuel, S., S. Ahmed, C. Fininsa, M. M. Abang and P. K. Sakhuja. 2008a. Survey of chocolate spot (Botrytis fabae) disease of faba bean (Vicia faba L.) and assessment of factors inﬂuencing disease epidemics in northern Ethiopia. Crop Protection, 27: 1457-1463.
[31]	Samuel, S., Chemeda, F., Sakhuja, P. K. and Seid, A., 2009. Evaluation of pathogenic isolates in Ethiopia for the control of Chocolate spot in faba bean. African Crop Sc. J., 17: 187–197.
[32]	Satish, S., Mohana, D. C., Ranhavendra, M. P. and Raveesha, K. A. 2007. Antifungal activity of some plant extracts against important seed borne pathogens of Aspergillus sp. J. of Agri. Technol., 3: 109-119.
[33]	Schmutterer, H., 1990. Properties and Potential of natural Pesticides from the neem tree, Azadirachta inidica. Annu. Rev. Entomol., 35: 271–97.
[34]	Shovan, L. R., Bhuiyan, M. K. A., Begum, J. A. and Pervez, Z., 2008. In vitro Control of Colletotrichum dematium Causing Anthracnose of Soybean by Fungicides, Plant Extracts and Trichoderma harzianum. Int. J. Sustain. Crop Prod., 3: 10-17.
[35]	Srivastava, J., J. Lambert and N. Vietmeyer, 1996. Medicinal plants: An expanding role in development. World Bank Technical Paper, No 320.
[36]	Suleiman, M. N., 2010. Fungitoxic Activity of Neem and Pawpaw Leaves Extracts on Alternaria Solani, Causal Organism of Yam Rots: Adv. Environ. Biol., 4: 159-161.
[37]	Sunder, A. R., Das, N. D. and Krishnaveni, D., 1995. In-vitro Antagonism of Trichoderma spp. against two Fungal Pathogens of Castor. Indian J. Plant Protec., 23: 152-155.
[38]	Torres, A. M., B. Roman, C. M. Avila, Z. Satovic, D. Rubiales, J. C. Sillero, J. I. Cubero and M. T. Moreno, 2006. Faba bean breeding for resistance against biotic stresses: towards application of marker technology. Euphytica., 147:67-80.
[39]	Tripathi, P., N. K. Dubey and A. K. Shukla, 2008. Use of some essential oils as postharvest botanical fungicides in the management of grey mould of grapes caused by Botrytis cinerea. World J Microbiol. Biotechnol., 24:39–46.
[40]	Wheeler, B. E. J., 1969. An introduction to plant diseases. WILEY, London, p. 347. 75 Williams, P. F., 1978. Growth of broad beans infected by Botrytis fabae. J. Hort. Sci., 50: 415-424
[41]	Yohannes Degago, 2000. Faba Bean (Vicia faba) in Ethiopia. Institute of Biodiversity, Conservation and Research (IBCR). Addis Ababa, Ethiopia. 43 p.
[42]	Zheng, J. Y., Lou, Q. S., Zheng, X. L. and Chen, B. L., 2008. Epidemics of chocolate spot of broad bean and control measures. J. Heilongjiang Agric. Sci., Issue 3: 161.

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Addisu Tegegn Tola, Meseret Chimdessa Egigu, Bekele Hundie Egdu. (2016). Bio-efficacy of Crude Leaf Extracts of Eucalyptus globulus Against In vitro and In vivo Growth of Chocolate Spot (Botrytis fabae Sard.) of Faba Bean (Vicia faba L.). Plant, 4(5), 37-44. https://doi.org/10.11648/j.plant.20160405.12

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Addisu Tegegn Tola; Meseret Chimdessa Egigu; Bekele Hundie Egdu. Bio-efficacy of Crude Leaf Extracts of Eucalyptus globulus Against In vitro and In vivo Growth of Chocolate Spot (Botrytis fabae Sard.) of Faba Bean (Vicia faba L.). Plant. 2016, 4(5), 37-44. doi: 10.11648/j.plant.20160405.12

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Addisu Tegegn Tola, Meseret Chimdessa Egigu, Bekele Hundie Egdu. Bio-efficacy of Crude Leaf Extracts of Eucalyptus globulus Against In vitro and In vivo Growth of Chocolate Spot (Botrytis fabae Sard.) of Faba Bean (Vicia faba L.). Plant. 2016;4(5):37-44. doi: 10.11648/j.plant.20160405.12

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@article{10.11648/j.plant.20160405.12,
  author = {Addisu Tegegn Tola and Meseret Chimdessa Egigu and Bekele Hundie Egdu},
  title = {Bio-efficacy of Crude Leaf Extracts of Eucalyptus globulus Against In vitro and In vivo Growth of Chocolate Spot (Botrytis fabae Sard.) of Faba Bean (Vicia faba L.)},
  journal = {Plant},
  volume = {4},
  number = {5},
  pages = {37-44},
  doi = {10.11648/j.plant.20160405.12},
  url = {https://doi.org/10.11648/j.plant.20160405.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.plant.20160405.12},
  abstract = {It is widely known that faba bean (Vicia faba L.) is seriously attacked by the fungal disease, chocolate spot, caused by Botrytis fabae resulting in a yield loss ranging from 50 to 100%. Even though synthetic fungicides are used as one of the effective options for the control of plant diseases, the environmental hazards and economic unfeasibility associated with them necessitate the search for relatively safe natural products. This study was initiated to evaluate the antifungal potential of crude extracts of leaves of Eucalyptus globulus, against in vitro and in vivo growth of Botrytis fabae. In the in vitro experiment, antifungal assay was set up using different concentrations of the crude extracts. In vivo experiment was conducted in the field by planting a faba bean variety, Shallo, (EH011-22-1) and selected plants from each plot were used for extract application and subsequent data collection. Laboratory experiment showed that, compared to the control, extracts obtained from each solvent managed to produce statistically significant (p <0.05) inhibition of mycelial growth. Based on the minimum concentration, inhibition from methanol extract (42.2%) significantly varied from that of ethanol (26.3%). Nevertheless, aqueous extract was nearly statistically the same to that of methanol. On the other hand, at maximum concentration (40%), a maximum inhibition percent of 83.7, which was nearly double of that produced by aqueous extract, was recorded from ethanol extract treated plates. Field experiment has shown that the use of ethanol extract of Eucalyptus globulus produced an efficacy of about 58.4%. Number of flowers aborted, number of tillers per m2, number of pods per plant, number of seeds per pod and hundred seeds weight were affected due to the disease suppression effect of the three extracts. However, there was quantitative variation in yield though no statistically significant difference was observed. The result of this study showed that extracts of the tested plant species have natural fungitoxic potential and showed a promising future for the development of safe natural alternative fungicides used for the control of Botrytis fabae after further pertinent tests and screening of the active principles.},
 year = {2016}
}

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TY  - JOUR
T1  - Bio-efficacy of Crude Leaf Extracts of Eucalyptus globulus Against In vitro and In vivo Growth of Chocolate Spot (Botrytis fabae Sard.) of Faba Bean (Vicia faba L.)
AU  - Addisu Tegegn Tola
AU  - Meseret Chimdessa Egigu
AU  - Bekele Hundie Egdu
Y1  - 2016/10/14
PY  - 2016
N1  - https://doi.org/10.11648/j.plant.20160405.12
DO  - 10.11648/j.plant.20160405.12
T2  - Plant
JF  - Plant
JO  - Plant
SP  - 37
EP  - 44
PB  - Science Publishing Group
SN  - 2331-0677
UR  - https://doi.org/10.11648/j.plant.20160405.12
AB  - It is widely known that faba bean (Vicia faba L.) is seriously attacked by the fungal disease, chocolate spot, caused by Botrytis fabae resulting in a yield loss ranging from 50 to 100%. Even though synthetic fungicides are used as one of the effective options for the control of plant diseases, the environmental hazards and economic unfeasibility associated with them necessitate the search for relatively safe natural products. This study was initiated to evaluate the antifungal potential of crude extracts of leaves of Eucalyptus globulus, against in vitro and in vivo growth of Botrytis fabae. In the in vitro experiment, antifungal assay was set up using different concentrations of the crude extracts. In vivo experiment was conducted in the field by planting a faba bean variety, Shallo, (EH011-22-1) and selected plants from each plot were used for extract application and subsequent data collection. Laboratory experiment showed that, compared to the control, extracts obtained from each solvent managed to produce statistically significant (p <0.05) inhibition of mycelial growth. Based on the minimum concentration, inhibition from methanol extract (42.2%) significantly varied from that of ethanol (26.3%). Nevertheless, aqueous extract was nearly statistically the same to that of methanol. On the other hand, at maximum concentration (40%), a maximum inhibition percent of 83.7, which was nearly double of that produced by aqueous extract, was recorded from ethanol extract treated plates. Field experiment has shown that the use of ethanol extract of Eucalyptus globulus produced an efficacy of about 58.4%. Number of flowers aborted, number of tillers per m2, number of pods per plant, number of seeds per pod and hundred seeds weight were affected due to the disease suppression effect of the three extracts. However, there was quantitative variation in yield though no statistically significant difference was observed. The result of this study showed that extracts of the tested plant species have natural fungitoxic potential and showed a promising future for the development of safe natural alternative fungicides used for the control of Botrytis fabae after further pertinent tests and screening of the active principles.
VL  - 4
IS  - 5
ER  -

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Author Information

Addisu Tegegn Tola

Pulse and Oil Crops Research Case Team, Sinana Agricultural Research Center, Bale Robe, Ethiopia
Meseret Chimdessa Egigu

Department of Biology, Haramaya University, Haramaya, Ethiopia
Bekele Hundie Egdu

Kulumsa Agricultural Research Center, Kulumsa, Ethiopia

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Addisu Tegegn Tola, Meseret Chimdessa Egigu, Bekele Hundie Egdu. (2016). Bio-efficacy of Crude Leaf Extracts of Eucalyptus globulus Against In vitro and In vivo Growth of Chocolate Spot (Botrytis fabae Sard.) of Faba Bean (Vicia faba L.). Plant, 4(5), 37-44. https://doi.org/10.11648/j.plant.20160405.12

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Addisu Tegegn Tola; Meseret Chimdessa Egigu; Bekele Hundie Egdu. Bio-efficacy of Crude Leaf Extracts of Eucalyptus globulus Against In vitro and In vivo Growth of Chocolate Spot (Botrytis fabae Sard.) of Faba Bean (Vicia faba L.). Plant. 2016, 4(5), 37-44. doi: 10.11648/j.plant.20160405.12

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AMA Style

Addisu Tegegn Tola, Meseret Chimdessa Egigu, Bekele Hundie Egdu. Bio-efficacy of Crude Leaf Extracts of Eucalyptus globulus Against In vitro and In vivo Growth of Chocolate Spot (Botrytis fabae Sard.) of Faba Bean (Vicia faba L.). Plant. 2016;4(5):37-44. doi: 10.11648/j.plant.20160405.12

Copy | Download

@article{10.11648/j.plant.20160405.12,
  author = {Addisu Tegegn Tola and Meseret Chimdessa Egigu and Bekele Hundie Egdu},
  title = {Bio-efficacy of Crude Leaf Extracts of Eucalyptus globulus Against In vitro and In vivo Growth of Chocolate Spot (Botrytis fabae Sard.) of Faba Bean (Vicia faba L.)},
  journal = {Plant},
  volume = {4},
  number = {5},
  pages = {37-44},
  doi = {10.11648/j.plant.20160405.12},
  url = {https://doi.org/10.11648/j.plant.20160405.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.plant.20160405.12},
  abstract = {It is widely known that faba bean (Vicia faba L.) is seriously attacked by the fungal disease, chocolate spot, caused by Botrytis fabae resulting in a yield loss ranging from 50 to 100%. Even though synthetic fungicides are used as one of the effective options for the control of plant diseases, the environmental hazards and economic unfeasibility associated with them necessitate the search for relatively safe natural products. This study was initiated to evaluate the antifungal potential of crude extracts of leaves of Eucalyptus globulus, against in vitro and in vivo growth of Botrytis fabae. In the in vitro experiment, antifungal assay was set up using different concentrations of the crude extracts. In vivo experiment was conducted in the field by planting a faba bean variety, Shallo, (EH011-22-1) and selected plants from each plot were used for extract application and subsequent data collection. Laboratory experiment showed that, compared to the control, extracts obtained from each solvent managed to produce statistically significant (p <0.05) inhibition of mycelial growth. Based on the minimum concentration, inhibition from methanol extract (42.2%) significantly varied from that of ethanol (26.3%). Nevertheless, aqueous extract was nearly statistically the same to that of methanol. On the other hand, at maximum concentration (40%), a maximum inhibition percent of 83.7, which was nearly double of that produced by aqueous extract, was recorded from ethanol extract treated plates. Field experiment has shown that the use of ethanol extract of Eucalyptus globulus produced an efficacy of about 58.4%. Number of flowers aborted, number of tillers per m2, number of pods per plant, number of seeds per pod and hundred seeds weight were affected due to the disease suppression effect of the three extracts. However, there was quantitative variation in yield though no statistically significant difference was observed. The result of this study showed that extracts of the tested plant species have natural fungitoxic potential and showed a promising future for the development of safe natural alternative fungicides used for the control of Botrytis fabae after further pertinent tests and screening of the active principles.},
 year = {2016}
}

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TY  - JOUR
T1  - Bio-efficacy of Crude Leaf Extracts of Eucalyptus globulus Against In vitro and In vivo Growth of Chocolate Spot (Botrytis fabae Sard.) of Faba Bean (Vicia faba L.)
AU  - Addisu Tegegn Tola
AU  - Meseret Chimdessa Egigu
AU  - Bekele Hundie Egdu
Y1  - 2016/10/14
PY  - 2016
N1  - https://doi.org/10.11648/j.plant.20160405.12
DO  - 10.11648/j.plant.20160405.12
T2  - Plant
JF  - Plant
JO  - Plant
SP  - 37
EP  - 44
PB  - Science Publishing Group
SN  - 2331-0677
UR  - https://doi.org/10.11648/j.plant.20160405.12
AB  - It is widely known that faba bean (Vicia faba L.) is seriously attacked by the fungal disease, chocolate spot, caused by Botrytis fabae resulting in a yield loss ranging from 50 to 100%. Even though synthetic fungicides are used as one of the effective options for the control of plant diseases, the environmental hazards and economic unfeasibility associated with them necessitate the search for relatively safe natural products. This study was initiated to evaluate the antifungal potential of crude extracts of leaves of Eucalyptus globulus, against in vitro and in vivo growth of Botrytis fabae. In the in vitro experiment, antifungal assay was set up using different concentrations of the crude extracts. In vivo experiment was conducted in the field by planting a faba bean variety, Shallo, (EH011-22-1) and selected plants from each plot were used for extract application and subsequent data collection. Laboratory experiment showed that, compared to the control, extracts obtained from each solvent managed to produce statistically significant (p <0.05) inhibition of mycelial growth. Based on the minimum concentration, inhibition from methanol extract (42.2%) significantly varied from that of ethanol (26.3%). Nevertheless, aqueous extract was nearly statistically the same to that of methanol. On the other hand, at maximum concentration (40%), a maximum inhibition percent of 83.7, which was nearly double of that produced by aqueous extract, was recorded from ethanol extract treated plates. Field experiment has shown that the use of ethanol extract of Eucalyptus globulus produced an efficacy of about 58.4%. Number of flowers aborted, number of tillers per m2, number of pods per plant, number of seeds per pod and hundred seeds weight were affected due to the disease suppression effect of the three extracts. However, there was quantitative variation in yield though no statistically significant difference was observed. The result of this study showed that extracts of the tested plant species have natural fungitoxic potential and showed a promising future for the development of safe natural alternative fungicides used for the control of Botrytis fabae after further pertinent tests and screening of the active principles.
VL  - 4
IS  - 5
ER  -

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