Study of Antagonistic Beneficial Microorganisms to Phytophtora colocasiae, Causal Agent of Taro Mildew (<i>Colocasia esculenta</i> (L.) Schott)

Asseng Charles Carnot; Ebongo Lobe Emmanuel; Nanda Djomou Giresse Ledoux; Akono Ntonga Patrick; Mbida Jean Arthur; Ngono Ngane Annie; Ambang Zachée; Monkam Tchamaha Fabrice; Djouokep Léonel Gautier

doi:doi:10.11648/j.plant.20170503.12

Study of Antagonistic Beneficial Microorganisms to Phytophtora colocasiae, Causal Agent of Taro Mildew (Colocasia esculenta (L.) Schott)

Asseng Charles Carnot, Ebongo Lobe Emmanuel, Nanda Djomou Giresse Ledoux, Akono Ntonga Patrick, Mbida Jean Arthur, Ngono Ngane Annie, Ambang Zachée, Monkam Tchamaha Fabrice, Djouokep Léonel Gautier

Published in Plant (Volume 5, Issue 3)

Received: 14 July 2017 Accepted: 21 July 2017 Published: 22 August 2017

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Abstract

The cultivation of taro is of great economic and social importance on a global scale. The current orientation towards agricultural production and the serious consequences of the mildew of taro to crops prompts to find alternatives to chemical control. This study aims at selecting in the taro habitat (leaves, rhizosphere) the microorganisms with high antagonistic potential capable of ensuring the biological control of P. colocasiae. Fungus isolated from the taro-infected leaves of the cultivar "Macumba or Ibo coco" from the V8-Agar medium, was kept in pure culture. The different antagonists were obtained by two trapping techniques using P. colocasiae as bait for the associated microorganisms and by the decimal dilution technique. The results reveal fourteen antagonist isolates, including five fungi and two bacteria isolated from the leaves; Against 4 bacteria and 3 fungi at ground level. Identification of the latter identified the presence of Penicillium Sp, Trichoderma Sp, Aspergillus Sp, Pythium Sp., Bacillus Sp, Rhizobium, Streptomyces and seven other unidentified isolates (Ni). The different in vitro tests showed that Rhizobium and Ni4 showed the strongest inhibitions (91.66 and 90.69%). The greenhouse tests showed the high-inhibitory effect of Trichoderma Sp. and Rhizobium, which showed very low foliar alteration percentages (9.65 and 1.86%). These antagonists would be of particular benefit to farmers in the development of biological pesticides.

Published in	Plant (Volume 5, Issue 3)
DOI	10.11648/j.plant.20170503.12
Page(s)	51-60
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Taro Mildew, Phytophtora colocasiae, Biological Control, Antagonist Microorganisms

References

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Asseng Charles Carnot, Ebongo Lobe Emmanuel, Nanda Djomou Giresse Ledoux, Akono Ntonga Patrick, Mbida Jean Arthur, et al. (2017). Study of Antagonistic Beneficial Microorganisms to Phytophtora colocasiae, Causal Agent of Taro Mildew (Colocasia esculenta (L.) Schott). Plant, 5(3), 51-60. https://doi.org/10.11648/j.plant.20170503.12

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Asseng Charles Carnot; Ebongo Lobe Emmanuel; Nanda Djomou Giresse Ledoux; Akono Ntonga Patrick; Mbida Jean Arthur, et al. Study of Antagonistic Beneficial Microorganisms to Phytophtora colocasiae, Causal Agent of Taro Mildew (Colocasia esculenta (L.) Schott). Plant. 2017, 5(3), 51-60. doi: 10.11648/j.plant.20170503.12

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Asseng Charles Carnot, Ebongo Lobe Emmanuel, Nanda Djomou Giresse Ledoux, Akono Ntonga Patrick, Mbida Jean Arthur, et al. Study of Antagonistic Beneficial Microorganisms to Phytophtora colocasiae, Causal Agent of Taro Mildew (Colocasia esculenta (L.) Schott). Plant. 2017;5(3):51-60. doi: 10.11648/j.plant.20170503.12

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@article{10.11648/j.plant.20170503.12,
  author = {Asseng Charles Carnot and Ebongo Lobe Emmanuel and Nanda Djomou Giresse Ledoux and Akono Ntonga Patrick and Mbida Jean Arthur and Ngono Ngane Annie and Ambang Zachée and Monkam Tchamaha Fabrice and Djouokep Léonel Gautier},
  title = {Study of Antagonistic Beneficial Microorganisms to Phytophtora colocasiae, Causal Agent of Taro Mildew (Colocasia esculenta (L.) Schott)},
  journal = {Plant},
  volume = {5},
  number = {3},
  pages = {51-60},
  doi = {10.11648/j.plant.20170503.12},
  url = {https://doi.org/10.11648/j.plant.20170503.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.plant.20170503.12},
  abstract = {The cultivation of taro is of great economic and social importance on a global scale. The current orientation towards agricultural production and the serious consequences of the mildew of taro to crops prompts to find alternatives to chemical control. This study aims at selecting in the taro habitat (leaves, rhizosphere) the microorganisms with high antagonistic potential capable of ensuring the biological control of P. colocasiae. Fungus isolated from the taro-infected leaves of the cultivar "Macumba or Ibo coco" from the V8-Agar medium, was kept in pure culture. The different antagonists were obtained by two trapping techniques using P. colocasiae as bait for the associated microorganisms and by the decimal dilution technique. The results reveal fourteen antagonist isolates, including five fungi and two bacteria isolated from the leaves; Against 4 bacteria and 3 fungi at ground level. Identification of the latter identified the presence of Penicillium Sp, Trichoderma Sp, Aspergillus Sp, Pythium Sp., Bacillus Sp, Rhizobium, Streptomyces and seven other unidentified isolates (Ni). The different in vitro tests showed that Rhizobium and Ni4 showed the strongest inhibitions (91.66 and 90.69%). The greenhouse tests showed the high-inhibitory effect of Trichoderma Sp. and Rhizobium, which showed very low foliar alteration percentages (9.65 and 1.86%). These antagonists would be of particular benefit to farmers in the development of biological pesticides.},
 year = {2017}
}

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TY - JOUR
T1 - Study of Antagonistic Beneficial Microorganisms to Phytophtora colocasiae, Causal Agent of Taro Mildew (Colocasia esculenta (L.) Schott)
AU - Asseng Charles Carnot
AU - Ebongo Lobe Emmanuel
AU - Nanda Djomou Giresse Ledoux
AU - Akono Ntonga Patrick
AU - Mbida Jean Arthur
AU - Ngono Ngane Annie
AU - Ambang Zachée
AU - Monkam Tchamaha Fabrice
AU - Djouokep Léonel Gautier
Y1 - 2017/08/22
PY - 2017
N1 - https://doi.org/10.11648/j.plant.20170503.12
DO - 10.11648/j.plant.20170503.12
T2 - Plant
JF - Plant
JO - Plant
SP - 51
EP - 60
PB - Science Publishing Group
SN - 2331-0677
UR - https://doi.org/10.11648/j.plant.20170503.12
AB - The cultivation of taro is of great economic and social importance on a global scale. The current orientation towards agricultural production and the serious consequences of the mildew of taro to crops prompts to find alternatives to chemical control. This study aims at selecting in the taro habitat (leaves, rhizosphere) the microorganisms with high antagonistic potential capable of ensuring the biological control of P. colocasiae. Fungus isolated from the taro-infected leaves of the cultivar "Macumba or Ibo coco" from the V8-Agar medium, was kept in pure culture. The different antagonists were obtained by two trapping techniques using P. colocasiae as bait for the associated microorganisms and by the decimal dilution technique. The results reveal fourteen antagonist isolates, including five fungi and two bacteria isolated from the leaves; Against 4 bacteria and 3 fungi at ground level. Identification of the latter identified the presence of Penicillium Sp, Trichoderma Sp, Aspergillus Sp, Pythium Sp., Bacillus Sp, Rhizobium, Streptomyces and seven other unidentified isolates (Ni). The different in vitro tests showed that Rhizobium and Ni4 showed the strongest inhibitions (91.66 and 90.69%). The greenhouse tests showed the high-inhibitory effect of Trichoderma Sp. and Rhizobium, which showed very low foliar alteration percentages (9.65 and 1.86%). These antagonists would be of particular benefit to farmers in the development of biological pesticides.
VL - 5
IS - 3
ER -

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Author Information

Asseng Charles Carnot

Faculty of Science, Department of Plant Biology, Laboratory of Plant Biology and Physiology, University of Douala, Douala, Cameroon
Ebongo Lobe Emmanuel

Faculty of Science, Department of Plant Biology, Laboratory of Plant Biology and Physiology, University of Douala, Douala, Cameroon
Nanda Djomou Giresse Ledoux

Faculty of Science, Department of Plant Biology, Laboratory of Plant Biology and Physiology, University of Douala, Douala, Cameroon
Akono Ntonga Patrick

Faculty of Science, Department of Plant Biology, Laboratory of Plant Biology and Physiology, University of Douala, Douala, Cameroon
Mbida Jean Arthur

Faculty of Science, Department of Plant Biology, Laboratory of Plant Biology and Physiology, University of Douala, Douala, Cameroon
Ngono Ngane Annie

Faculty of Science, Department of Plant Biology, Laboratory of Plant Biology and Physiology, University of Douala, Douala, Cameroon
Ambang Zachée

Faculty of Science, Department of Plant Biology, Laboratory of Phytopathology and Microbiology, University of Yaounde, Yaounde, Cameroon
Monkam Tchamaha Fabrice

Faculty of Science, Department of Plant Biology, Laboratory of Plant Biology and Physiology, University of Douala, Douala, Cameroon
Djouokep Léonel Gautier

Faculty of Science, Department of Plant Biology, Laboratory of Plant Biology and Physiology, University of Douala, Douala, Cameroon

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Asseng Charles Carnot, Ebongo Lobe Emmanuel, Nanda Djomou Giresse Ledoux, Akono Ntonga Patrick, Mbida Jean Arthur, et al. (2017). Study of Antagonistic Beneficial Microorganisms to Phytophtora colocasiae, Causal Agent of Taro Mildew (Colocasia esculenta (L.) Schott). Plant, 5(3), 51-60. https://doi.org/10.11648/j.plant.20170503.12

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Asseng Charles Carnot; Ebongo Lobe Emmanuel; Nanda Djomou Giresse Ledoux; Akono Ntonga Patrick; Mbida Jean Arthur, et al. Study of Antagonistic Beneficial Microorganisms to Phytophtora colocasiae, Causal Agent of Taro Mildew (Colocasia esculenta (L.) Schott). Plant. 2017, 5(3), 51-60. doi: 10.11648/j.plant.20170503.12

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AMA Style

Asseng Charles Carnot, Ebongo Lobe Emmanuel, Nanda Djomou Giresse Ledoux, Akono Ntonga Patrick, Mbida Jean Arthur, et al. Study of Antagonistic Beneficial Microorganisms to Phytophtora colocasiae, Causal Agent of Taro Mildew (Colocasia esculenta (L.) Schott). Plant. 2017;5(3):51-60. doi: 10.11648/j.plant.20170503.12

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@article{10.11648/j.plant.20170503.12,
  author = {Asseng Charles Carnot and Ebongo Lobe Emmanuel and Nanda Djomou Giresse Ledoux and Akono Ntonga Patrick and Mbida Jean Arthur and Ngono Ngane Annie and Ambang Zachée and Monkam Tchamaha Fabrice and Djouokep Léonel Gautier},
  title = {Study of Antagonistic Beneficial Microorganisms to Phytophtora colocasiae, Causal Agent of Taro Mildew (Colocasia esculenta (L.) Schott)},
  journal = {Plant},
  volume = {5},
  number = {3},
  pages = {51-60},
  doi = {10.11648/j.plant.20170503.12},
  url = {https://doi.org/10.11648/j.plant.20170503.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.plant.20170503.12},
  abstract = {The cultivation of taro is of great economic and social importance on a global scale. The current orientation towards agricultural production and the serious consequences of the mildew of taro to crops prompts to find alternatives to chemical control. This study aims at selecting in the taro habitat (leaves, rhizosphere) the microorganisms with high antagonistic potential capable of ensuring the biological control of P. colocasiae. Fungus isolated from the taro-infected leaves of the cultivar "Macumba or Ibo coco" from the V8-Agar medium, was kept in pure culture. The different antagonists were obtained by two trapping techniques using P. colocasiae as bait for the associated microorganisms and by the decimal dilution technique. The results reveal fourteen antagonist isolates, including five fungi and two bacteria isolated from the leaves; Against 4 bacteria and 3 fungi at ground level. Identification of the latter identified the presence of Penicillium Sp, Trichoderma Sp, Aspergillus Sp, Pythium Sp., Bacillus Sp, Rhizobium, Streptomyces and seven other unidentified isolates (Ni). The different in vitro tests showed that Rhizobium and Ni4 showed the strongest inhibitions (91.66 and 90.69%). The greenhouse tests showed the high-inhibitory effect of Trichoderma Sp. and Rhizobium, which showed very low foliar alteration percentages (9.65 and 1.86%). These antagonists would be of particular benefit to farmers in the development of biological pesticides.},
 year = {2017}
}

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