Antioxidant and Antibacterial Activity of Organic Extracts of Roots of <i>Glycyrrhiza Glabra Linn</i>

Zafar Iqbal; Zhang Hai; Hong Yan Ping; Abdul Ghaffar; Munaza Mumtaz; Luban Liaqat

doi:doi:10.11648/j.plant.20170504.12

| Peer-Reviewed

Antioxidant and Antibacterial Activity of Organic Extracts of Roots of Glycyrrhiza Glabra Linn

Zafar Iqbal, Zhang Hai, Hong Yan Ping, Abdul Ghaffar, Munaza Mumtaz, Luban Liaqat

Published in Plant (Volume 5, Issue 4)

Received: 7 March 2017 Accepted: 27 March 2017 Published: 30 October 2017

Views: Downloads:

Download PDF

Share This Article

Twitter
Linked In
Facebook

Abstract

Extacts of root of Glycyrrhiza glabra linn were prepared by using n-hexan, toluene, tetrahydrofuran, ethylacetate, and ethanol and their antioxidant and antimicrobial activities were measured. Secondary metalbilites like flavonoids, alkaloids, terpenoids, glycosides, and steroids were measured and it was found that highly polar solvents ike ethyl alcohol, ethyl acetate and THF extract more metabolites as compared to less polar solvents. In vitro antioxidant activity of organic extracts of glycyrrhiza glabra root was measured by using DPPH scavenging radicle. Ethanolic extract gave highest antioxidant activity of 92% followed by ethyl acetate 89%, THF 87%, toluene 45% and hexane 23% respectively. The organic extracts of the plant root was further tested for its antibacterial activity against two bacteria viz. Staphylococcus aureus and Escherichia coli. Ethanolic extract and THF extracts have showed mild antibacterial activity against saphylococcus aureus and escherichia coli respectively whereas n-hexane extract failed to inhibit the growth of bacteria. The results have shown that organic extract of Glycyrrhiza glabra linn is rich source of secondary metabolites, a strong antioxidant and possess mild antibacterial activity.

Published in	Plant (Volume 5, Issue 4)
DOI	10.11648/j.plant.20170504.12
Page(s)	68-72
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Glycyrrhiza Glabra Linn, Orgnic Extracts, Antioxidant, Antibacterials

References

[1]	RT. Nair, J. Kalaria,, S. Chanda, Antibacterial activity of some selected Indian medicinal flora. Turk. j. boil., 2005; 29: 41-47.
[2]	M. Stiffness, J. Douros, Current status of the NCL plants and animal product programme. Natural Product., 1982; 45: 1-45.
[3]	K. Rajandeep, K. Harpreet, S. Ajaib, A. Dhindsa, Glycyrrhiza glabra: a phyto pharmacological review. Inter J. Pharma. Sci. Res., 2013; 4(7): 2470-2477.
[4]	K. Ajay, Meena, S. Arjun, S. Kiran, S. Kumara, MM. Rao, Physicochemical and preliminary phytochemical studies on the rhizomes of glycyrrhiza glabra linn, Inter. J. Pharmacy Pharma. Sci., 2010; 2(2): 48-49.
[5]	R. Asha, K. Navneet, V. Chaudhari, S. Kumar, C. Vikash, P. Devendra, S. Manoj, K. Panday, Phytochemical constituent, pharmacological activities and medicinal uses through the millennia of glycyrrhiza glabra linn: A review. Inter. Res. J. pharmacy, 2012; 3(8): 45-55.
[6]	A. Olukoga, D. Donaldson, Historical perspectives on health, the history of liquorice: The plant, its extract, cultivation, and commercialization and etymology. J. Res. Soc health, 1998; 18: 300-304.
[7]	T. Lakhshimi, R. Geetha, Glycyrrhiza glabra linn commonly known as licorice a therapeutic review. Inter. J. Pharmacy Pharma. Sci., 2011; 3 (4): 20-25.
[8]	S. Sanjai, (2005). Glycyrrhiza glabra: Medicine over the millennium. Nat. Prod. Radiance., 1999; 4(5): 358-367.
[9]	S. Lata, R. S. Saxena, A. Kumar, S. Kakkar, V. K. Srivastava, K. K. Saxena, Comparative antipyretic activity of ocimum sanctum, glycyrrhiza glabra and aspirin in experimentally induced pyrexia in rats. Ind. J. Pharmacology. 1999; 31: 71-75.
[10]	I. Amna, A. Barkat, KB. Sajid, Glycyrrhiza glabra extract cream: Effects on skin pigment “Melanin”. international conference on bioscience, biochemistry, bioinformatics PCBEE, ACSIT Press Singapore. (2011); 5: 434-439.
[11]	S. Rajarshi, R. Doel, SA. Chakraborti, Glycyrrhizin ameliorates insulin resistant, hyperglycemia dyslipidemia and oxidative stress in fructose induced metabolic syndrome-X in rats. Ind. J. Exper. Biol., 2013; 5: 129-138.
[12]	VK. Gupta, A. Fatima, N. Faridi, AS. Shankar, K. Kumar, JK. Rahuja, N. Luqman, S. Sisodia, BS. Saikia, D. Darokar, MP. Khanuja, Antimicrobial potential of glycyrrhiza glabra roots. J. Ethnopharmacology. 2008; 116: 377-380.
[13]	U. Sukiriti, K. Ashoke, GS. Vijender, Hair growth promotant activity of petroleum ether root extract of glycyrrhiza glabra L (Fabaceae) in female rats. Trop. J. Pharma. Res., 2012; 5: 753-758.
[14]	M. Seedi, KM. Semmani, MR. Ghoreishe, The treatment of atopic dermatitis with licorice gel. J Dermatol Treat., 2003;14: 153-157.
[15]	T. Minglei, HY Yuan, HR. Kyung, Extraction of glycyrrhizic acid and glabridin from licorice, Inter. J. Mol. Sci., 2008; 9: 571-577.
[16]	H. Michael, J. Gordon, Antioxidant activity of flavonoids isolated from licorice. J. Agric. Food Chem., 1995; 43: 1784-1788.
[17]	LA. Mitscher, YH. Park, D. Clark, JL. Beal, Antimicrobial agents from the higher plants, antimicrobial isoflavonoids and related substances from glycyrrhiza glabra linn. Var. Typical, J. Nat. Prod. 1980; 43: 259-262.
[18]	T. Fukai, A. Marumo, K. Kaitou, T. Kanda, S. Terada, T. Monura, Anti-helicobacter pylori flavonoids from licorice extracts. Life Sci., 2002; 71: 1449-1463.
[19]	PKP. Gaitry, C. Binda, DS. Farhin, I. Sujata, S. Deo, Antimicrobial and antioxidant activities of methanol extract roots of glycyrrhiza and HPLC analysis. Inter. J. Pharmacy Pharma. Sci., 2013; 5(2): 157-160.
[20]	NR. Fransworth, Biological and phytochemical screening of plants. J. Pharma. Sci., 1966; 55(3): 225-276.
[21]	E. Iqbal, KA. Salin, BL. Linda, Phytochemical screening, total phenolics and antioxidant activities of bark and leaf extracts of goniothalamus velatinus ( Airy Shaw) from Brunei Darussala. J. king Saud Univ. Sci., 2015; 27: 224-232.
[22]	JC. Epsin, CS. Rivas, HJ. Wiches, Characterization of the total free radical scavenging capacity of vegetable oil and oil fractions using 2,2-diphenyl-1-picrylhydrazyl radical. J. Agri. Food. Chem., 2000;48: 648-656.
[23]	V. Sharma, RC. Agrawal, S. Pandey, Phytochemical screening and determination of anti-bacterial and anti-oxidant potential of glycyrrhiza glabra root extracts. J. Environ. Res. Devel., 2013; 7(4A): 1552-1558.
[24]	HH. Wijngaad, N. Brunton, The optimization of solid-liquid extraction of antioxidants from the apple pomace by response surface methodolgy, J. Food Eng., 2010; 96(1): 134-140.
[25]	HN. Rajha, NE. Darra, Z. Hobaika, N. boussetta, E. Vorobiev, RG. Maroun, N. Louka, Extraction of total phenolic compounds, flavonoids, anthocyanins and tannins from grap by-products by response surface methodology. Influence of solid-liquid ratio, particle size, time, temperature, and solvent mixture on the optimization process. Food Nutr. Sci., 2014; 5: 397-409.
[26]	S. Sianne, RVH. Fanie, Antimalarial activity of plant metabolites. Nat. Prod. Rep., 2002; 19: 675-692.
[27]	T. Murugan, JA. Wins, M. Murugan, Antibacterial activity and phytochemical constituents of leaf extract of cassia auriculata, Indian J. Pharm. Sci., 2013; 75(1): 122-125.
[28]	O. Poltrai, Antioxidants and free radicals scavengers of natural origin, Current Org. Chem., 1997; 1: 415-440.
[29]	S. Demizu, K. Kajiyama, Y. Takahashi, S. Hiraga, Y. Yamamoto, K. Tamura, TO. Kinoshita. Antioxidant and antimicrobial constituents of licorice: isolation and structure elucidation of a new benzofuran derivative. Chem. Pharm. Bull., 1988; 36: 3474‐3479.
[30]	MM. Cowan, Plant products as antimicrobial agents, Clin. Microbiol. Rev., 1999;12:564–82.
[31]	M. Manoj, KC. Nitalikar, M. Balaji, V. Dhore, N. Sajid, Shikalgar, Studies of antibacterial activities of glycyrrhiza glabra root extract. Int. J. Pharm. Tech. Res., 2010; 2(1): 899-901.
[32]	RM. Zablotowicz, RE. Hoagland, SC Wagner, Effect of saponins on the growth and activity of rhizosphere bacteria, Adv. Exp. Med. Biol., 1996; 405:83–95.
[33]	SM. Patil, MB. Patil, GN. Sapkale, Antimicrobial activity of glycyrrhiza glabra linn roots. Int. J. Chem. Sci., 2009; 7(1): 585-591.
[34]	T. Shimada, Salivary proteins as a defense against dietary tannins, J. Chem. Ecol., 2006; 32:1149–63.

Cite This Article

Plain Text BibTeX RIS

APA Style

Zafar Iqbal, Zhang Hai, Hong Yan Ping, Abdul Ghaffar, Munaza Mumtaz, et al. (2017). Antioxidant and Antibacterial Activity of Organic Extracts of Roots of Glycyrrhiza Glabra Linn. Plant, 5(4), 68-72. https://doi.org/10.11648/j.plant.20170504.12

Copy | Download

ACS Style

Zafar Iqbal; Zhang Hai; Hong Yan Ping; Abdul Ghaffar; Munaza Mumtaz, et al. Antioxidant and Antibacterial Activity of Organic Extracts of Roots of Glycyrrhiza Glabra Linn. Plant. 2017, 5(4), 68-72. doi: 10.11648/j.plant.20170504.12

Copy | Download

AMA Style

Zafar Iqbal, Zhang Hai, Hong Yan Ping, Abdul Ghaffar, Munaza Mumtaz, et al. Antioxidant and Antibacterial Activity of Organic Extracts of Roots of Glycyrrhiza Glabra Linn. Plant. 2017;5(4):68-72. doi: 10.11648/j.plant.20170504.12

Copy | Download

@article{10.11648/j.plant.20170504.12,
  author = {Zafar Iqbal and Zhang Hai and Hong Yan Ping and Abdul Ghaffar and Munaza Mumtaz and Luban Liaqat},
  title = {Antioxidant and Antibacterial Activity of Organic Extracts of Roots of Glycyrrhiza Glabra Linn},
  journal = {Plant},
  volume = {5},
  number = {4},
  pages = {68-72},
  doi = {10.11648/j.plant.20170504.12},
  url = {https://doi.org/10.11648/j.plant.20170504.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.plant.20170504.12},
  abstract = {Extacts of root of Glycyrrhiza glabra linn were prepared by using n-hexan, toluene, tetrahydrofuran, ethylacetate, and ethanol and their antioxidant and antimicrobial activities were measured. Secondary metalbilites like flavonoids, alkaloids, terpenoids, glycosides, and steroids were measured and it was found that highly polar solvents ike ethyl alcohol, ethyl acetate and THF extract more metabolites as compared to less polar solvents. In vitro antioxidant activity of organic extracts of glycyrrhiza glabra root was measured by using DPPH scavenging radicle. Ethanolic extract gave highest antioxidant activity of 92% followed by ethyl acetate 89%, THF 87%, toluene 45% and hexane 23% respectively. The organic extracts of the plant root was further tested for its antibacterial activity against two bacteria viz. Staphylococcus aureus and Escherichia coli. Ethanolic extract and THF extracts have showed mild antibacterial activity against saphylococcus aureus and escherichia coli respectively whereas n-hexane extract failed to inhibit the growth of bacteria. The results have shown that organic extract of Glycyrrhiza glabra linn is rich source of secondary metabolites, a strong antioxidant and possess mild antibacterial activity.},
 year = {2017}
}

Copy | Download

TY  - JOUR
T1  - Antioxidant and Antibacterial Activity of Organic Extracts of Roots of Glycyrrhiza Glabra Linn
AU  - Zafar Iqbal
AU  - Zhang Hai
AU  - Hong Yan Ping
AU  - Abdul Ghaffar
AU  - Munaza Mumtaz
AU  - Luban Liaqat
Y1  - 2017/10/30
PY  - 2017
N1  - https://doi.org/10.11648/j.plant.20170504.12
DO  - 10.11648/j.plant.20170504.12
T2  - Plant
JF  - Plant
JO  - Plant
SP  - 68
EP  - 72
PB  - Science Publishing Group
SN  - 2331-0677
UR  - https://doi.org/10.11648/j.plant.20170504.12
AB  - Extacts of root of Glycyrrhiza glabra linn were prepared by using n-hexan, toluene, tetrahydrofuran, ethylacetate, and ethanol and their antioxidant and antimicrobial activities were measured. Secondary metalbilites like flavonoids, alkaloids, terpenoids, glycosides, and steroids were measured and it was found that highly polar solvents ike ethyl alcohol, ethyl acetate and THF extract more metabolites as compared to less polar solvents. In vitro antioxidant activity of organic extracts of glycyrrhiza glabra root was measured by using DPPH scavenging radicle. Ethanolic extract gave highest antioxidant activity of 92% followed by ethyl acetate 89%, THF 87%, toluene 45% and hexane 23% respectively. The organic extracts of the plant root was further tested for its antibacterial activity against two bacteria viz. Staphylococcus aureus and Escherichia coli. Ethanolic extract and THF extracts have showed mild antibacterial activity against saphylococcus aureus and escherichia coli respectively whereas n-hexane extract failed to inhibit the growth of bacteria. The results have shown that organic extract of Glycyrrhiza glabra linn is rich source of secondary metabolites, a strong antioxidant and possess mild antibacterial activity.
VL  - 5
IS  - 4
ER  -

Copy | Download

Author Information

Zafar Iqbal

Applied Chemistry Research Centre, PCSIR Laboratories Complex, Lahore, Pakistan
Zhang Hai

School of Chemistry and Chemical Engineering, Qianbei Featured Resources, Applied Research Key Laboratory, Zunyi Normal College, Guizhou, Zunyi, China
Hong Yan Ping

Jiangxi Key Laboratory of Natural Product and Functional Food, College of Food Science and Engineering, Jiangxi, Agriculutural University, Nanchang, China
Abdul Ghaffar

Department of Applied Chemistry, University of Engineering and Technology, Lahore, Pakistan
Munaza Mumtaz

Department of Applied Chemistry, University of Engineering and Technology, Lahore, Pakistan
Luban Liaqat

Applied Chemistry Research Centre, PCSIR Laboratories Complex, Lahore, Pakistan

Download PDF

Sections

Plain Text BibTeX RIS

APA Style

Zafar Iqbal, Zhang Hai, Hong Yan Ping, Abdul Ghaffar, Munaza Mumtaz, et al. (2017). Antioxidant and Antibacterial Activity of Organic Extracts of Roots of Glycyrrhiza Glabra Linn. Plant, 5(4), 68-72. https://doi.org/10.11648/j.plant.20170504.12

Copy | Download

ACS Style

Zafar Iqbal; Zhang Hai; Hong Yan Ping; Abdul Ghaffar; Munaza Mumtaz, et al. Antioxidant and Antibacterial Activity of Organic Extracts of Roots of Glycyrrhiza Glabra Linn. Plant. 2017, 5(4), 68-72. doi: 10.11648/j.plant.20170504.12

Copy | Download

AMA Style

Zafar Iqbal, Zhang Hai, Hong Yan Ping, Abdul Ghaffar, Munaza Mumtaz, et al. Antioxidant and Antibacterial Activity of Organic Extracts of Roots of Glycyrrhiza Glabra Linn. Plant. 2017;5(4):68-72. doi: 10.11648/j.plant.20170504.12

Copy | Download

@article{10.11648/j.plant.20170504.12,
  author = {Zafar Iqbal and Zhang Hai and Hong Yan Ping and Abdul Ghaffar and Munaza Mumtaz and Luban Liaqat},
  title = {Antioxidant and Antibacterial Activity of Organic Extracts of Roots of Glycyrrhiza Glabra Linn},
  journal = {Plant},
  volume = {5},
  number = {4},
  pages = {68-72},
  doi = {10.11648/j.plant.20170504.12},
  url = {https://doi.org/10.11648/j.plant.20170504.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.plant.20170504.12},
  abstract = {Extacts of root of Glycyrrhiza glabra linn were prepared by using n-hexan, toluene, tetrahydrofuran, ethylacetate, and ethanol and their antioxidant and antimicrobial activities were measured. Secondary metalbilites like flavonoids, alkaloids, terpenoids, glycosides, and steroids were measured and it was found that highly polar solvents ike ethyl alcohol, ethyl acetate and THF extract more metabolites as compared to less polar solvents. In vitro antioxidant activity of organic extracts of glycyrrhiza glabra root was measured by using DPPH scavenging radicle. Ethanolic extract gave highest antioxidant activity of 92% followed by ethyl acetate 89%, THF 87%, toluene 45% and hexane 23% respectively. The organic extracts of the plant root was further tested for its antibacterial activity against two bacteria viz. Staphylococcus aureus and Escherichia coli. Ethanolic extract and THF extracts have showed mild antibacterial activity against saphylococcus aureus and escherichia coli respectively whereas n-hexane extract failed to inhibit the growth of bacteria. The results have shown that organic extract of Glycyrrhiza glabra linn is rich source of secondary metabolites, a strong antioxidant and possess mild antibacterial activity.},
 year = {2017}
}

Copy | Download

TY  - JOUR
T1  - Antioxidant and Antibacterial Activity of Organic Extracts of Roots of Glycyrrhiza Glabra Linn
AU  - Zafar Iqbal
AU  - Zhang Hai
AU  - Hong Yan Ping
AU  - Abdul Ghaffar
AU  - Munaza Mumtaz
AU  - Luban Liaqat
Y1  - 2017/10/30
PY  - 2017
N1  - https://doi.org/10.11648/j.plant.20170504.12
DO  - 10.11648/j.plant.20170504.12
T2  - Plant
JF  - Plant
JO  - Plant
SP  - 68
EP  - 72
PB  - Science Publishing Group
SN  - 2331-0677
UR  - https://doi.org/10.11648/j.plant.20170504.12
AB  - Extacts of root of Glycyrrhiza glabra linn were prepared by using n-hexan, toluene, tetrahydrofuran, ethylacetate, and ethanol and their antioxidant and antimicrobial activities were measured. Secondary metalbilites like flavonoids, alkaloids, terpenoids, glycosides, and steroids were measured and it was found that highly polar solvents ike ethyl alcohol, ethyl acetate and THF extract more metabolites as compared to less polar solvents. In vitro antioxidant activity of organic extracts of glycyrrhiza glabra root was measured by using DPPH scavenging radicle. Ethanolic extract gave highest antioxidant activity of 92% followed by ethyl acetate 89%, THF 87%, toluene 45% and hexane 23% respectively. The organic extracts of the plant root was further tested for its antibacterial activity against two bacteria viz. Staphylococcus aureus and Escherichia coli. Ethanolic extract and THF extracts have showed mild antibacterial activity against saphylococcus aureus and escherichia coli respectively whereas n-hexane extract failed to inhibit the growth of bacteria. The results have shown that organic extract of Glycyrrhiza glabra linn is rich source of secondary metabolites, a strong antioxidant and possess mild antibacterial activity.
VL  - 5
IS  - 4
ER  -

Copy | Download