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Determination of Antiemetic, Antimicrobial, Anti-Radical and Cytotoxic Activity of Methanolic Extracts of Centella asiatica

Received: 05 March 2018    Accepted: 24 March 2018    Published: 13 April 2018
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Abstract

This study was conducted to investigate the antiemetic, antimicrobial, antioxidant and cytotoxic activity of methanolic extracts of Centella asiatica. The antiemetic assay was carried out by using chick emetic model with minor modifications by calculating the mean decrease in the number of retching. The antimicrobial activity of the crude extract was performed by Disc Diffusion method. The anti-radical activity was determined by the 2,2-diphenyl-2-picrylhydrazyl hydrate (DPPH) method. Brine shrimp lethality bioassay is done to determine cytotoxic activity. The anti-emetic activity of Centella asiatica leaves on young chicks revealed that these extracts have a less anti-emetic effect. The group of chicks treated with Chlorpromazine was found to have 38.4 retches as compared to the 60.4 retches of the control group, thus Chlorpromazine reduced the retches by 36.96%. The chickens treated with leaves extracts inhibited the retches up to 15.67%. The manimum antimicrobial effect was found in this methanol crude extract. The extract did not appear potent in terms of both zones of inhibition and spectrum of activity. In anti-radical activity test, the extract showed moderate free radical scavenging activity with IC50 value 241.71 μg/ml. while compared to that of the reference standard ascorbic acid. Moreover, the methanolic crude extracts also possess moderate cytotoxic principles potential (LC50 value of 39.06 μg/ml) comparing with that of standard vincristine (0.839 μg/ml).

DOI 10.11648/j.plant.20180601.11
Published in Plant (Volume 6, Issue 1, March 2018)
Page(s) 1-7
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Antiemetic, Antimicrobial, Antioxidant, Cytotoxicity, Centella asiatica

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Author Information
  • Department of Pharmacy, Manarat International University, Dhaka, Bangladesh

  • Department of Pharmacy, Manarat International University, Dhaka, Bangladesh

  • Department of Pharmacy, Manarat International University, Dhaka, Bangladesh

  • Department of Pharmacy, Manarat International University, Dhaka, Bangladesh

  • Department of Pharmacy, Manarat International University, Dhaka, Bangladesh

  • Department of Pharmacy, University of Asia Pacific, Dhaka, Bangladesh

  • Department of Pharmacy, Atish Dipankar University of Science and Technology, Dhaka, Bangladesh

  • Department of Pharmacy, Atish Dipankar University of Science and Technology, Dhaka, Bangladesh

  • Department of Pharmacy, State University of Bangladesh, Dhaka, Bangladesh

Cite This Article
  • APA Style

    Md. Shohel Hossain, Fahmida Abdullah Tuly, Sharmin Akter, Md Saiful Islam Arman, Md. Abdul Aziz, et al. (2018). Determination of Antiemetic, Antimicrobial, Anti-Radical and Cytotoxic Activity of Methanolic Extracts of Centella asiatica. Plant, 6(1), 1-7. https://doi.org/10.11648/j.plant.20180601.11

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    ACS Style

    Md. Shohel Hossain; Fahmida Abdullah Tuly; Sharmin Akter; Md Saiful Islam Arman; Md. Abdul Aziz, et al. Determination of Antiemetic, Antimicrobial, Anti-Radical and Cytotoxic Activity of Methanolic Extracts of Centella asiatica. Plant. 2018, 6(1), 1-7. doi: 10.11648/j.plant.20180601.11

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    AMA Style

    Md. Shohel Hossain, Fahmida Abdullah Tuly, Sharmin Akter, Md Saiful Islam Arman, Md. Abdul Aziz, et al. Determination of Antiemetic, Antimicrobial, Anti-Radical and Cytotoxic Activity of Methanolic Extracts of Centella asiatica. Plant. 2018;6(1):1-7. doi: 10.11648/j.plant.20180601.11

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  • @article{10.11648/j.plant.20180601.11,
      author = {Md. Shohel Hossain and Fahmida Abdullah Tuly and Sharmin Akter and Md Saiful Islam Arman and Md. Abdul Aziz and Md. Ekhtear Mahmud and Palash Das and Mohammad Hasem Babu and Md. Monirul Islam},
      title = {Determination of Antiemetic, Antimicrobial, Anti-Radical and Cytotoxic Activity of Methanolic Extracts of Centella asiatica},
      journal = {Plant},
      volume = {6},
      number = {1},
      pages = {1-7},
      doi = {10.11648/j.plant.20180601.11},
      url = {https://doi.org/10.11648/j.plant.20180601.11},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.plant.20180601.11},
      abstract = {This study was conducted to investigate the antiemetic, antimicrobial, antioxidant and cytotoxic activity of methanolic extracts of Centella asiatica. The antiemetic assay was carried out by using chick emetic model with minor modifications by calculating the mean decrease in the number of retching. The antimicrobial activity of the crude extract was performed by Disc Diffusion method. The anti-radical activity was determined by the 2,2-diphenyl-2-picrylhydrazyl hydrate (DPPH) method. Brine shrimp lethality bioassay is done to determine cytotoxic activity. The anti-emetic activity of Centella asiatica leaves on young chicks revealed that these extracts have a less anti-emetic effect. The group of chicks treated with Chlorpromazine was found to have 38.4 retches as compared to the 60.4 retches of the control group, thus Chlorpromazine reduced the retches by 36.96%. The chickens treated with leaves extracts inhibited the retches up to 15.67%. The manimum antimicrobial effect was found in this methanol crude extract. The extract did not appear potent in terms of both zones of inhibition and spectrum of activity. In anti-radical activity test, the extract showed moderate free radical scavenging activity with IC50 value 241.71 μg/ml. while compared to that of the reference standard ascorbic acid. Moreover, the methanolic crude extracts also possess moderate cytotoxic principles potential (LC50 value of 39.06 μg/ml) comparing with that of standard vincristine (0.839 μg/ml).},
     year = {2018}
    }
    

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  • TY  - JOUR
    T1  - Determination of Antiemetic, Antimicrobial, Anti-Radical and Cytotoxic Activity of Methanolic Extracts of Centella asiatica
    AU  - Md. Shohel Hossain
    AU  - Fahmida Abdullah Tuly
    AU  - Sharmin Akter
    AU  - Md Saiful Islam Arman
    AU  - Md. Abdul Aziz
    AU  - Md. Ekhtear Mahmud
    AU  - Palash Das
    AU  - Mohammad Hasem Babu
    AU  - Md. Monirul Islam
    Y1  - 2018/04/13
    PY  - 2018
    N1  - https://doi.org/10.11648/j.plant.20180601.11
    DO  - 10.11648/j.plant.20180601.11
    T2  - Plant
    JF  - Plant
    JO  - Plant
    SP  - 1
    EP  - 7
    PB  - Science Publishing Group
    SN  - 2331-0677
    UR  - https://doi.org/10.11648/j.plant.20180601.11
    AB  - This study was conducted to investigate the antiemetic, antimicrobial, antioxidant and cytotoxic activity of methanolic extracts of Centella asiatica. The antiemetic assay was carried out by using chick emetic model with minor modifications by calculating the mean decrease in the number of retching. The antimicrobial activity of the crude extract was performed by Disc Diffusion method. The anti-radical activity was determined by the 2,2-diphenyl-2-picrylhydrazyl hydrate (DPPH) method. Brine shrimp lethality bioassay is done to determine cytotoxic activity. The anti-emetic activity of Centella asiatica leaves on young chicks revealed that these extracts have a less anti-emetic effect. The group of chicks treated with Chlorpromazine was found to have 38.4 retches as compared to the 60.4 retches of the control group, thus Chlorpromazine reduced the retches by 36.96%. The chickens treated with leaves extracts inhibited the retches up to 15.67%. The manimum antimicrobial effect was found in this methanol crude extract. The extract did not appear potent in terms of both zones of inhibition and spectrum of activity. In anti-radical activity test, the extract showed moderate free radical scavenging activity with IC50 value 241.71 μg/ml. while compared to that of the reference standard ascorbic acid. Moreover, the methanolic crude extracts also possess moderate cytotoxic principles potential (LC50 value of 39.06 μg/ml) comparing with that of standard vincristine (0.839 μg/ml).
    VL  - 6
    IS  - 1
    ER  - 

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