Response of Lentil Genotypes Under PEG-induced Drought Stress: Effect on Germination and Growth

Chrysanthi Foti; Ebrahim Khah; Ourania Pavli

doi:doi:10.11648/j.plant.20180604.12

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Response of Lentil Genotypes Under PEG-induced Drought Stress: Effect on Germination and Growth

Chrysanthi Foti, Ebrahim Khah, Ourania Pavli

Published in Plant (Volume 6, Issue 4)

Received: 17 November 2018 Accepted: 5 December 2018 Published: 28 January 2019

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Abstract

Drought has a negative impact on plant growth and is responsible for considerable crop yield loses worldwide. Given the importance of improving yield under drought, the ability to select tolerant genetic material is a prerequisite in all relevant plant breeding activities. Lentil is an economically important crop which often suffers from inadequate soil moisture. In this study, seed germination potential and seedling growth were determined in various genotypes exposed to drought as a means to explore the possibility of identifying drought-tolerant germplasm at an early stage. Drought stress experiments were carried out using six lentil cultivars, representing local and imported germplasm. Stress was induced by varying concentrations of polyethylene glycol (PEG6000: 0%, 5%, 10% and 20%). Genotype performance was assessed on a daily basis and referred to germination percentage (%), seed water absorbance (%), seedling water content (%), shoot and root length (cm) and number of seedlings with abnormal genotype. Our findings revealed that drought stress substantially affects parameters associated to germination and growth, with its effect being analogous to the stress level applied. Genotypic differences also were evident, with cultivars Elpida, Samos and Thessalia proving as the most tolerant and cultivar Flip 03-24L as the least tolerant genotypes under severe drought stress. Overall findings provide evidence that identifying drought tolerant germplasm might be accomplished by scoring seed germination and early growth potential under water deficit conditions. Such possibility is of outmost importance for a time- and cost-efficient selection of drought tolerant lentil genotypes to be exploited in breeding programs.

Published in	Plant (Volume 6, Issue 4)
DOI	10.11648/j.plant.20180604.12
Page(s)	75-83
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Abiotic Stress Tolerance, Drought, Lentil, PEG6000, Early Selection

References

[1]	Zohary, D. (1972). The wild progenitor and place of origin of the cultivated lentil Lens culinaris. Economic Botany, 26, 326-332.
[2]	Erskine, W., Rihawi, S. and Capper, B. S. (1990). Variation in lentil straw quality. Animal Feed Science Technology, 28, 61–69.
[3]	Erskine, W., Muehlbauer, F. J., Sarker, A. and Sharma, B. (2009). Introduction. In: W. Erskine, F. J. Muehlbauer, A. Sarker and B. Sharma (Eds.), The lentil: botany, production and uses (pp. 1-3). Oxfordshire: CAB International.
[4]	Muehlbauer, F. J., Cho, S., Sarker, A., McPhee, K. E., Coyne, C. J., Rajesh, P. N. and Ford, R. (2006). Application of biotechnology in breeding lentil for resistance to biotic and abiotic stress. Euphytica, 147, 149-165.
[5]	Malhotra, R. S., Sarker, A. and Saxena, M. C. (2004). Drought tolerance in chickpea and lentil-present status and future strategies. In: S. C. Rao & J. Ryan (Eds.), Challenges and strategies for dryland agriculture. (pp. 257–273). Wisconsin: CSSA Special Publication N. 32.
[6]	Sarker, A., Aydogan, A., Chandra, S., Kharrat, M. and Sabaghpour, S. (2009). Genetic enhancement for yield and yield stability. In: W. Erskine, F. J. Muehlbauer, A. Sarker and Sharma B. (Eds.), The lentil: botany, production and uses. (pp. 102–120). Oxfordshire: CAB International.
[7]	Idrissi, O., Houasli, C., Udupa, S. M., De Keyser, E., Van Damme, P. and Riek, J. D. (2015). Genetic variability for root and shoot traits in a lentil (Lens culinaris Medik.) recombinant inbred line population and their association with drought tolerance. Euphytica, 204, 693–709.
[8]	Silim, S. N., Saxena, M. C. and Erskine, W. (1993). Adaptation of lentil to the Mediterranean environment. II. Response to moisture supply. Experimental Agriculture, 29, 21-28.
[9]	Khourgami, A., Maghooli, E., Rafiee, M. and Bitarafan, Z. (2012). Lentil response to supplementary irrigation and plant density under dry farming condition. International Journal of Science and Advanced Technology, 2, 51-55.
[10]	Salehi, M., Haghnazari, A., Shekari, F. and Faramarzi, A. (2008). The study of seed yield and seed yield components of lentil (Lens culinaris Medik) under normal and drought stress conditions. Pakistan Journal of Biological Science, 11, 758-762.
[11]	Muscolo, A., Sidari, M., Anastasi, U., Santonoceto, C. and Maggio, A. (2014). Effect of PEG-induced drought stress on seed germination of four lentil genotypes. Journal of Plant Interactions, 9, 354-363.
[12]	Cattivelli, L., Rizza, F., Badeck, F. W., Mazzucotelli, E., Mastrangelo, A. M., Francia, E., Mare, C., Tondelli, A. and Stanca M. (2008). Drought tolerance improvement in crop plants: An integrated view from breeding to genomics. Field Crops Research, 105, 1-14.
[13]	Tuberosa, R. (2012). Phenotyping for drought tolerance of crops in the genomics era. Frontiers in Physiology, 3, 1-26.
[14]	Kpoghomou, B. K., Sapra, V. T. and Beyl, C. A. (1990). Screening for drought tolerance: Soybean germination and its relationship to seedling responses. Journal of Agronomy and Crop Science, 164, 153–159.
[15]	Grzesiak, S., Filek, W., Skrudlik, G. and Niziol, B. (1996). Screening for drought tolerance: Evaluation of seed germination and seedling growth for drought resistance in legume plants. Journal of Agronomy and Crop Science, 177, 245–252.
[16]	Govindaraj, M. Shanmugasundaram, P. Sumathi, P. and Muthiah, A. (2010). Simple, rapid and cost effective screening method for drought resistant breeding in pearl millet. Electronic Journal of Plant Breeding, 1, 590-599.
[17]	Kaufmann, E. and Eckard, A. (1970). Evaluation of water stress control with polyethylene glycols by analysis of guttation. Plant Physiology, 47, 453-456.
[18]	Steuter, A. (1981). Water potential of aqueous polyethylene glycol. Plant Physiology, 67, 64-67.
[19]	Kulkarni, M. and Deshpande, U. (2007). In vitro screening of tomato genotypes for drought resistance using polyethylene glycol. African Journal of Biotechnology, 6, 691-696.
[20]	Mujeeb‐ur‐Rahman Soomro U. A., Zahoor‐ul‐Haq M. and Gul S. (2008) Effects of NaCl salinity on wheat (Triticum aestivum L.) cultivars. World Journal of Agricultural Sciences, 4, 398–403.
[21]	Black, M. and Pritchard, H. (2002). Desiccation and survival in plants drying without dying. (pp. 93-110). New York: CABI publishing.
[22]	Singh, B., Reddy, K. R., Redoña, E. D. and Walker, T. (2017). Developing a screening tool for osmotic stress tolerance classification of rice cultivars based on in vitro seed germination. Crop Science, 57, 387-394.
[23]	Thabet, S. Moursi, Y. Karam, M. Graner, A. and Alqudah, A. (2018). Genetic basis of drought tolerance during seed germination in barley. Plos One, https://doi.org/10.1371/journal.pone.0206682.
[24]	Kaya, M., Okcu, G., Atak, M., Cıkılı, Y. and Kolsarıcı, O. (2006). Seed treatments to overcome salt and drought stress during germination in sunflower (Helianthus annuus L.). European Journal of Agronomy, 24, 291–295.
[25]	Sadeghi, H., Khazaei, F., Yari, L. and Sheidaei, S. (2011). Effect of seed osmopriming on seed germination behavior and vigor of soybean (Glycine max L.). ARPN Journal of Agricultural and Biological Science, 6, 39-43.
[26]	Bewley, J. D. (1997). Seed germination and dormancy. Plant Cell, (9), 1055-1066.
[27]	Bewley, J. D., Bradford, K. J., Hilhorst, H. W. M. and Nonogaki, H. (2013). Seeds. In: Bewley, J. D., Bradford, K. J., Hilhorst, H. W. M. & Nonogaki, H. (Eds.), Physiology of Development, Germination and Dormancy (pp. 133-183). London: Springer New York Heidelberg Dordrecht.
[28]	Awatif, A. and Alaaeldin, E. (2017). Metabolic Processes During Seed Germination. In: Jimenez-Lopez, J. (Ed.), Advances in Seed Biology. IntechOpen. doi.org/10.5772/intechopen.70653.
[29]	Foti, C., Khah, E. M., and Pavli, O. I. (2018) Germination profiling of lentil genotypes subjected to salinity stress. Plant Biology, https://doi.org/10.1111/plb.12714.
[30]	Sidari, M., Santonoceto, C., Anastasi, U., Preiti, G. and Muscolo, A. (2008). Variations in four genotypes of lentil under NaCl-salinity stress. American Journal of Agricultural and Biological Sciences, 3, 410-4.
[31]	Muscolo, A. Junker, A. Klukas, C. Weigelt-Fischer, K. Riewe, D. Altmann, T. (2015). Phenotypic and metabolic responses to drought and salinity of four contrasting lentil accessions. Journal of Experimental Botany, 18, 5467-5480.

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APA Style

Chrysanthi Foti, Ebrahim Khah, Ourania Pavli. (2019). Response of Lentil Genotypes Under PEG-induced Drought Stress: Effect on Germination and Growth. Plant, 6(4), 75-83. https://doi.org/10.11648/j.plant.20180604.12

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ACS Style

Chrysanthi Foti; Ebrahim Khah; Ourania Pavli. Response of Lentil Genotypes Under PEG-induced Drought Stress: Effect on Germination and Growth. Plant. 2019, 6(4), 75-83. doi: 10.11648/j.plant.20180604.12

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AMA Style

Chrysanthi Foti, Ebrahim Khah, Ourania Pavli. Response of Lentil Genotypes Under PEG-induced Drought Stress: Effect on Germination and Growth. Plant. 2019;6(4):75-83. doi: 10.11648/j.plant.20180604.12

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@article{10.11648/j.plant.20180604.12,
  author = {Chrysanthi Foti and Ebrahim Khah and Ourania Pavli},
  title = {Response of Lentil Genotypes Under PEG-induced Drought Stress: Effect on Germination and Growth},
  journal = {Plant},
  volume = {6},
  number = {4},
  pages = {75-83},
  doi = {10.11648/j.plant.20180604.12},
  url = {https://doi.org/10.11648/j.plant.20180604.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.plant.20180604.12},
  abstract = {Drought has a negative impact on plant growth and is responsible for considerable crop yield loses worldwide. Given the importance of improving yield under drought, the ability to select tolerant genetic material is a prerequisite in all relevant plant breeding activities. Lentil is an economically important crop which often suffers from inadequate soil moisture. In this study, seed germination potential and seedling growth were determined in various genotypes exposed to drought as a means to explore the possibility of identifying drought-tolerant germplasm at an early stage. Drought stress experiments were carried out using six lentil cultivars, representing local and imported germplasm. Stress was induced by varying concentrations of polyethylene glycol (PEG6000: 0%, 5%, 10% and 20%). Genotype performance was assessed on a daily basis and referred to germination percentage (%), seed water absorbance (%), seedling water content (%), shoot and root length (cm) and number of seedlings with abnormal genotype. Our findings revealed that drought stress substantially affects parameters associated to germination and growth, with its effect being analogous to the stress level applied. Genotypic differences also were evident, with cultivars Elpida, Samos and Thessalia proving as the most tolerant and cultivar Flip 03-24L as the least tolerant genotypes under severe drought stress. Overall findings provide evidence that identifying drought tolerant germplasm might be accomplished by scoring seed germination and early growth potential under water deficit conditions. Such possibility is of outmost importance for a time- and cost-efficient selection of drought tolerant lentil genotypes to be exploited in breeding programs.},
 year = {2019}
}

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TY  - JOUR
T1  - Response of Lentil Genotypes Under PEG-induced Drought Stress: Effect on Germination and Growth
AU  - Chrysanthi Foti
AU  - Ebrahim Khah
AU  - Ourania Pavli
Y1  - 2019/01/28
PY  - 2019
N1  - https://doi.org/10.11648/j.plant.20180604.12
DO  - 10.11648/j.plant.20180604.12
T2  - Plant
JF  - Plant
JO  - Plant
SP  - 75
EP  - 83
PB  - Science Publishing Group
SN  - 2331-0677
UR  - https://doi.org/10.11648/j.plant.20180604.12
AB  - Drought has a negative impact on plant growth and is responsible for considerable crop yield loses worldwide. Given the importance of improving yield under drought, the ability to select tolerant genetic material is a prerequisite in all relevant plant breeding activities. Lentil is an economically important crop which often suffers from inadequate soil moisture. In this study, seed germination potential and seedling growth were determined in various genotypes exposed to drought as a means to explore the possibility of identifying drought-tolerant germplasm at an early stage. Drought stress experiments were carried out using six lentil cultivars, representing local and imported germplasm. Stress was induced by varying concentrations of polyethylene glycol (PEG6000: 0%, 5%, 10% and 20%). Genotype performance was assessed on a daily basis and referred to germination percentage (%), seed water absorbance (%), seedling water content (%), shoot and root length (cm) and number of seedlings with abnormal genotype. Our findings revealed that drought stress substantially affects parameters associated to germination and growth, with its effect being analogous to the stress level applied. Genotypic differences also were evident, with cultivars Elpida, Samos and Thessalia proving as the most tolerant and cultivar Flip 03-24L as the least tolerant genotypes under severe drought stress. Overall findings provide evidence that identifying drought tolerant germplasm might be accomplished by scoring seed germination and early growth potential under water deficit conditions. Such possibility is of outmost importance for a time- and cost-efficient selection of drought tolerant lentil genotypes to be exploited in breeding programs.
VL  - 6
IS  - 4
ER  -

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Author Information

Chrysanthi Foti

Laboratory of Genetics and Plant Breeding, Department of Agriculture, Crop Production and Rural Environment, School of Agricultural Science, University of Thessaly, Volos, Greece
Ebrahim Khah

Laboratory of Genetics and Plant Breeding, Department of Agriculture, Crop Production and Rural Environment, School of Agricultural Science, University of Thessaly, Volos, Greece
Ourania Pavli

Laboratory of Genetics and Plant Breeding, Department of Agriculture, Crop Production and Rural Environment, School of Agricultural Science, University of Thessaly, Volos, Greece

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APA Style

Chrysanthi Foti, Ebrahim Khah, Ourania Pavli. (2019). Response of Lentil Genotypes Under PEG-induced Drought Stress: Effect on Germination and Growth. Plant, 6(4), 75-83. https://doi.org/10.11648/j.plant.20180604.12

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ACS Style

Chrysanthi Foti; Ebrahim Khah; Ourania Pavli. Response of Lentil Genotypes Under PEG-induced Drought Stress: Effect on Germination and Growth. Plant. 2019, 6(4), 75-83. doi: 10.11648/j.plant.20180604.12

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AMA Style

Chrysanthi Foti, Ebrahim Khah, Ourania Pavli. Response of Lentil Genotypes Under PEG-induced Drought Stress: Effect on Germination and Growth. Plant. 2019;6(4):75-83. doi: 10.11648/j.plant.20180604.12

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@article{10.11648/j.plant.20180604.12,
  author = {Chrysanthi Foti and Ebrahim Khah and Ourania Pavli},
  title = {Response of Lentil Genotypes Under PEG-induced Drought Stress: Effect on Germination and Growth},
  journal = {Plant},
  volume = {6},
  number = {4},
  pages = {75-83},
  doi = {10.11648/j.plant.20180604.12},
  url = {https://doi.org/10.11648/j.plant.20180604.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.plant.20180604.12},
  abstract = {Drought has a negative impact on plant growth and is responsible for considerable crop yield loses worldwide. Given the importance of improving yield under drought, the ability to select tolerant genetic material is a prerequisite in all relevant plant breeding activities. Lentil is an economically important crop which often suffers from inadequate soil moisture. In this study, seed germination potential and seedling growth were determined in various genotypes exposed to drought as a means to explore the possibility of identifying drought-tolerant germplasm at an early stage. Drought stress experiments were carried out using six lentil cultivars, representing local and imported germplasm. Stress was induced by varying concentrations of polyethylene glycol (PEG6000: 0%, 5%, 10% and 20%). Genotype performance was assessed on a daily basis and referred to germination percentage (%), seed water absorbance (%), seedling water content (%), shoot and root length (cm) and number of seedlings with abnormal genotype. Our findings revealed that drought stress substantially affects parameters associated to germination and growth, with its effect being analogous to the stress level applied. Genotypic differences also were evident, with cultivars Elpida, Samos and Thessalia proving as the most tolerant and cultivar Flip 03-24L as the least tolerant genotypes under severe drought stress. Overall findings provide evidence that identifying drought tolerant germplasm might be accomplished by scoring seed germination and early growth potential under water deficit conditions. Such possibility is of outmost importance for a time- and cost-efficient selection of drought tolerant lentil genotypes to be exploited in breeding programs.},
 year = {2019}
}

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TY  - JOUR
T1  - Response of Lentil Genotypes Under PEG-induced Drought Stress: Effect on Germination and Growth
AU  - Chrysanthi Foti
AU  - Ebrahim Khah
AU  - Ourania Pavli
Y1  - 2019/01/28
PY  - 2019
N1  - https://doi.org/10.11648/j.plant.20180604.12
DO  - 10.11648/j.plant.20180604.12
T2  - Plant
JF  - Plant
JO  - Plant
SP  - 75
EP  - 83
PB  - Science Publishing Group
SN  - 2331-0677
UR  - https://doi.org/10.11648/j.plant.20180604.12
AB  - Drought has a negative impact on plant growth and is responsible for considerable crop yield loses worldwide. Given the importance of improving yield under drought, the ability to select tolerant genetic material is a prerequisite in all relevant plant breeding activities. Lentil is an economically important crop which often suffers from inadequate soil moisture. In this study, seed germination potential and seedling growth were determined in various genotypes exposed to drought as a means to explore the possibility of identifying drought-tolerant germplasm at an early stage. Drought stress experiments were carried out using six lentil cultivars, representing local and imported germplasm. Stress was induced by varying concentrations of polyethylene glycol (PEG6000: 0%, 5%, 10% and 20%). Genotype performance was assessed on a daily basis and referred to germination percentage (%), seed water absorbance (%), seedling water content (%), shoot and root length (cm) and number of seedlings with abnormal genotype. Our findings revealed that drought stress substantially affects parameters associated to germination and growth, with its effect being analogous to the stress level applied. Genotypic differences also were evident, with cultivars Elpida, Samos and Thessalia proving as the most tolerant and cultivar Flip 03-24L as the least tolerant genotypes under severe drought stress. Overall findings provide evidence that identifying drought tolerant germplasm might be accomplished by scoring seed germination and early growth potential under water deficit conditions. Such possibility is of outmost importance for a time- and cost-efficient selection of drought tolerant lentil genotypes to be exploited in breeding programs.
VL  - 6
IS  - 4
ER  -

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