Plant

| Peer-Reviewed |

Study of Symbiotic Pathogenic Microorganisms Associated with Phytophthora colocasiae Causal Agent of Taro Mildew (Colocasia esculenta (L.) Schott)

Received: 16 July 2017    Accepted: 16 August 2017    Published: 10 October 2017
Views:       Downloads:

Share This Article

Abstract

Symbiotic pathogenic microorganisms associated with P. colocasiae leading to the manifestation of taro mildew have been identified and studied. The experiment was carried out in an experimental set-up consisting of completely randomized blocks with three treatments (infection with the pure strain, with the unpurified strain and a co-infection with both strains). Isolation and purification of the microorganisms was carried out in the PDA medium and the identification on the analysis of the macroscopic and microscopic morphological characters. The results of the trapping revealed among several a single microorganism which has a high affinity with P. colocasiae, this microorganism named Vorticella is identified as the agent of the amplification of the severity of the downy mildew. Greenhouse studies of taro plants showed that the unpurified strain caused enough damage with 96.67% disease incidence and a severity of 70.18% compared to plants inoculated with pure strain which had an incidence of 40% and a severity of 10.48%. All these results show that P. colocasiae is the causal agent of the mildew of the taro in the infection and the development of the disease; it is accompanied by other microorganisms with which it forms a biofilm. In this biofilm there is a microorganism which plays a very important role in the spread of the disease and it is thanks to it that all parts of the plant are attacked.

DOI 10.11648/j.plant.s.2017050501.15
Published in Plant (Volume 5, Issue 5-1, October 2017)

This article belongs to the Special Issue Phytotherapy

Page(s) 33-42
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

P. colocasiae, C. esculenta, Symbiotic Microorganisms, Vorticella

References
[1] Irwin S. V., P. Kaufusi, K. Banks, R. de la Pena, J. J. Cho, 1998. Molecular characterization of taro (Colocasia esculenta) using RAPD markers. Euphytica 99: 183-189, Kluwer Academic Publishers. Printed in the Netherlands.
[2] Mishra PK, S. Mishra, G. Selvakumar, SC. Bisht, S. Kundu, JK. Bisht, HS. Gupta, (2008) Characterization of a psychrotrophic plant growth promoting Pseudomonas PGERs17 (MTCC9000) isolated from North Western Indian Himalayas. Annals of Microbiology 58 (4): 1-8.
[3] FAOSTAT. 2011. FAO Economic and Social Department. The Statistics Division. Major Food and Agricultural Commodities and Producers. http://faostat.fao.org/default.aspx.
[4] Scot N., Brooks F. E. et Glenn T. (2011). Taro Leaf Blight in Hawaii. University of Hawaii at Mänoa, Plant Disease 71: 1-14.
[5] IITA 2009. Root and Tuber systems. http://www.iita.org/cms/articlefiles/2009. Ivancic A. et Lebot V. (1999). Botany and genetics of New Caledonian wild taro, Colocasia esculenta. Pacifie science, Vol. 53, no. 3,273-285.
[6] Anjana Rao, Yongqiang Zhang, Sabina Muend, Rajini Rao, 2010. Mechanism of Antifungal Activity of Terpenoid Phenols Resembles Calcium Stress and Inhibition of the TOR Pathway. doi: 10.1128/AAC.01050-10 Antimicrob. Agents Chemother. December 2010 vol. 54 no. 12 5062-5069.
[7] FAO (2011). Production du taro. Base de données de FAOSTAT. http://WWW.FAO.org/ingho/.
[8] Onwueme, I. C., 1978. The tropical tuber crops. Yams, cassava, sweet potato, cocoyams. John Wiley et Sons, Chichester, United Kingdom. 234 pp.
[9] Caillon S. (2005). Les taros du Vanuatu: que conserver et comment? Natures Sciences Sociétés 13: 306-310.
[10] Misra R. S, Sriram S. (2002). Medicinal value and export potential of tropical tuber crops. In: Govil J. N, Pandey J, Shivkumar B. G. and Singh V. K. (Editions.). Series Recent Progress in Medicinal Plants, Crop Improvement, Production and Commerce. USA.376-386pp.
[11] Binoy B., Vinayaka H., Makeshkumar T. & Jeeva M. L.2010. Rapid Detection and Identification of Potyvirus Infecting Colocasia esculenta (L.) Schott by Reverse Transcription-Polymerase Chain Reaction. Journal of Root Crops 36 (1):88-94.
[12] Wang J. (1983). Taro: a review of Colocasiae esculenta and its potentials. University of Hawaii Press. Honolulu, Hawaii 400p.
[13] L. (2010). Taro Leaf Blight in Cameroon. Biodiversity Weblog. Accessed December 3, 2015. Available at: http:/agro.biodiverse/2010/07/taro-leaf blight-in-Cameroon.
[14] Fullerton R. A. et Tyson J. L. 2004. The biology of Phytophthora colocasiae and implications for its management and control. Pp. 107-111. In: Secretariat of the Pacific Community (Ed.). Third Taro Symposium, 2003. Nadi, Fiji Islands. 39. CTA (Centre Technique de Coopération Agricole et Rurale) 2010. Guide d’exportation pour les plantes à racines et tubercules en Afrique de l’Ouest et du Centre. Dakar, Sénégal. 32p.
[15] Brooks, F. E. (2005). Taro leaf blight. The Plant Health Instructor. DOI:10.1094/PHI-I-2005-0531-01.
[16] Misra R. S. (2000). Integrated management of Phytophthora leaf blight of taro. Proc. Indian Phytopathology. Golden Jubilee 2: 1183-1184.
[17] Djeugap, J. F., Fontem, D. A. & Tapondjou, A. L. 2009. Évaluation des milieux de culture pour la croissance de Phytophthora infestans, agent causal du mildiou chez la morelle noire. Biosciences Proceedings, 15: 85-92.
[18] Zhu J, Zhang Z, Yang Z. 2001. General research methods on pathogen of potato late blight (Phytophthora infestans). Journal of Agricultural Sciences, 24: 112-114.
[19] Byrd, A. L. and Segre, J. A. (2016). Adapting Koch's postulates. Science, 351(6270): 224-226.
[20] Adomako, J., Kwoseh, C. K. Moses, E. and Larbi-Koranteng S. (2016). Prevalence of Phytophthora Leaf Blight of Taro (Colocasia esculenta (L.) Schott) in the Semi Deciduous Forest Zone of Ghana. AJEA, 11(4): 1-7.
[21] Lu, H. Y., Wei, M. L., Lu, C. T., and Chan, L. F. (2002). Comparison of Different Models for Non-destructive Leaf Area Estimation in Taro. Journal of Agronomy, 96:448-453.
[22] Chaube, H. S and Pundhir, V. S. (2005). Crop Diseases and Their Management. Printice – Hall of India New Delhi – 11001. 703 pp.
[23] Omeje, T. E., Ugwuoke, K. I., Adinde, J. O., Ogwulumba, S. I. and Unigwe, L. O. (2016). Effect of cropping season on the control of Taro Leaf Blight (Phytophthora colocasiae) of cocoyam (Colocasia esculenta L.) in Nsukka, south eastern Nigeria, I. J. A. B. R, 6(1): 30-39.
[24] Aurelie Lecellier, 2013. Caractérisation et identification des champignons filamenteux par spectroscopie vibrationnelle. Thèse de Doctorat. Université de Reims Champagne-Ardeme.
[25] Mbong G. A., Fokunang C. N., Manju E. B., Njukeng A. P., Tembe-Fokunang E. A., Itanna Rachid, 2015. Mycelia growth and sporulation of Phytopthora colocasiae isolates under selected conditions. Amer. J. Exp. Agric. 8(4), 193-201.
[26] Nath V. S., Sankar M. S., Hegde V. M., Jeeva M. L., Misra R. S. and Veena S. S. 2013. Molecular evidence supports hypervariability in Phytophthora colocasiae associated with leaf blight of taro. European J. Plant Pathol., 136: 483–494.
[27] Tsopmbeng Noumbo Gaston, Megatche christien Jean Pitagor, Lienou Jules Appolinaire, Yaouba Aoudou, Djeugap Fovo Joseph and Fontem Dominic Ajong (2014). Évaluation des activités antifongiques des extraits de plantes contre Phytophthora colocasiae, agent causal du mildiou du taro (Colocasia esculenta (L.) Schott). Journal of Applied Biosciences 81:7221–7232.
[28] Ho HH, Ann PJ, Chang HS. 1995. The Genus Phytophthora in Taiwan, Monograph Series No 15. Taipei, Taiwan, Institute of Botany. Academia Sinica.
[29] Erwin DC, Ribeiro OK, 1996. Phytophthora Disases Worldwide. American Phytopathological Society, APS Press: St Paul, MN, USA. 562 p.
[30] Jean T. Coulibaly, Eliezer K. N’Goran, Jury Utzinger, Michael J. Doenhoff, Emily M. Dawson, 2013. A new rapid diagnostic test for detection of anti-Schristosoma mansoni and anti-Schistosoma haematobium antibodies. Parasites and vectors 6 (2013).
[31] Eric Galiana, Antoine Marais, Catherine Mura, Benoît Industri, Gilles Arbiol and Michel Ponchet (2011). Ecosystem Screening Approach for Pathogen-Associated Microorganisms Affecting Host Disease. Applied and Environmental Microbiology, 77 (17): p. 6069–6075.
[32] Wolinska, J., and K. C. King. 2009. Environment can alter selection in host-parasite interactions. Trends Parasitol. 25:236–244.
[33] Ravva S. V., Sarreal C. Z., Mandrell R. E. (2010). Identification of protozoa in dairy lagoon wastewater that consume Escherichia coli O157:H7 preferentially. PLoS ONE 5:e15671 10.1371/journal.pone.0015671.
[34] Mbong G. A., Fokunang C. N., Fontem, L. A.,Bambot M. B. and Tembe E. A., 2013. An overview of Phytophthora colocasiae of cocoyams: A potential economic disease of food security in Cameroon. Discourse J. Agric. Food Sci. 1(9): 140-145.
[35] Adinde, J. O., Anieke, U. J., Nwankwo, O. G., Agu, C. J., Aniakor, A. C., Nwagboso, A. A., Eze, C. O., 2016. Incidence and severity of taro leaf-blight in Iwollo, South-Eastern Nigeria. Int. J. Curr. Res. Biosci. Plant Biol. 3(10), 163-168. doi: http://dx.doi.org/10.20546/ijcrbp.2016.310.020.
[36] Asseng Charles Carnot, Mvoe Christian Roger, Ambang Zachée and Monkam Tchamaha Fabrice. (2016). Influence of the Number of Watering and Fungicide Treatments on the Development of Phytophthora colocasiae (Racib) on Cocoyam (Xanthosoma sagittifolium) and Taro (Colocasia esculenta) Greenhouse in Cameroon. International Journal of Current Microbiology and Applied Sciences Volume 5 Number 8 pp. 100-112.
Author Information
  • Faculty of Science, Department of Plant Biology, Laboratory of Plant Biology and Physiology, University of Douala, Douala, Cameroon

  • Faculty of Science, Department of Plant Biology, Laboratory of Plant Biology and Physiology, University of Douala, Douala, Cameroon

  • Faculty of Science, Department of Plant Biology, Laboratory of Plant Biology and Physiology, University of Douala, Douala, Cameroon

  • Faculty of Science, Department of Plant Biology, Laboratory of Plant Biology and Physiology, University of Douala, Douala, Cameroon

  • Faculty of Science, Department of Plant Biology, Laboratory of Plant Biology and Physiology, University of Douala, Douala, Cameroon

  • Faculty of Science, Department of Plant Biology, Laboratory of Plant Biology and Physiology, University of Douala, Douala, Cameroon

  • Faculty of Science, Department of Plant Biology, Laboratory of Phytopathology and Microbiology, University of Yaounde, Yaounde, Cameroon

  • Faculty of Science, Department of Plant Biology, Laboratory of Plant Biology and Physiology, University of Douala, Douala, Cameroon

  • Faculty of Science, Department of Plant Biology, Laboratory of Plant Biology and Physiology, University of Douala, Douala, Cameroon

Cite This Article
  • APA Style

    Asseng Charles Carnot, Nanda Djomou Giresse Ledoux, Ebongo Lobe Emmanuel, Akono Ntonga Patrick, Mbida Jean Arthur, et al. (2017). Study of Symbiotic Pathogenic Microorganisms Associated with Phytophthora colocasiae Causal Agent of Taro Mildew (Colocasia esculenta (L.) Schott). Plant, 5(5-1), 33-42. https://doi.org/10.11648/j.plant.s.2017050501.15

    Copy | Download

    ACS Style

    Asseng Charles Carnot; Nanda Djomou Giresse Ledoux; Ebongo Lobe Emmanuel; Akono Ntonga Patrick; Mbida Jean Arthur, et al. Study of Symbiotic Pathogenic Microorganisms Associated with Phytophthora colocasiae Causal Agent of Taro Mildew (Colocasia esculenta (L.) Schott). Plant. 2017, 5(5-1), 33-42. doi: 10.11648/j.plant.s.2017050501.15

    Copy | Download

    AMA Style

    Asseng Charles Carnot, Nanda Djomou Giresse Ledoux, Ebongo Lobe Emmanuel, Akono Ntonga Patrick, Mbida Jean Arthur, et al. Study of Symbiotic Pathogenic Microorganisms Associated with Phytophthora colocasiae Causal Agent of Taro Mildew (Colocasia esculenta (L.) Schott). Plant. 2017;5(5-1):33-42. doi: 10.11648/j.plant.s.2017050501.15

    Copy | Download

  • @article{10.11648/j.plant.s.2017050501.15,
      author = {Asseng Charles Carnot and Nanda Djomou Giresse Ledoux and Ebongo Lobe Emmanuel and Akono Ntonga Patrick and Mbida Jean Arthur and Ngono Ngane Annie and Ambang Zachée and Monkam Tchamaha Fabrice and Djouokep Léonel Gautier},
      title = {Study of Symbiotic Pathogenic Microorganisms Associated with Phytophthora colocasiae Causal Agent of Taro Mildew (Colocasia esculenta (L.) Schott)},
      journal = {Plant},
      volume = {5},
      number = {5-1},
      pages = {33-42},
      doi = {10.11648/j.plant.s.2017050501.15},
      url = {https://doi.org/10.11648/j.plant.s.2017050501.15},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.plant.s.2017050501.15},
      abstract = {Symbiotic pathogenic microorganisms associated with P. colocasiae leading to the manifestation of taro mildew have been identified and studied. The experiment was carried out in an experimental set-up consisting of completely randomized blocks with three treatments (infection with the pure strain, with the unpurified strain and a co-infection with both strains). Isolation and purification of the microorganisms was carried out in the PDA medium and the identification on the analysis of the macroscopic and microscopic morphological characters. The results of the trapping revealed among several a single microorganism which has a high affinity with P. colocasiae, this microorganism named Vorticella is identified as the agent of the amplification of the severity of the downy mildew. Greenhouse studies of taro plants showed that the unpurified strain caused enough damage with 96.67% disease incidence and a severity of 70.18% compared to plants inoculated with pure strain which had an incidence of 40% and a severity of 10.48%. All these results show that P. colocasiae is the causal agent of the mildew of the taro in the infection and the development of the disease; it is accompanied by other microorganisms with which it forms a biofilm. In this biofilm there is a microorganism which plays a very important role in the spread of the disease and it is thanks to it that all parts of the plant are attacked.},
     year = {2017}
    }
    

    Copy | Download

  • TY  - JOUR
    T1  - Study of Symbiotic Pathogenic Microorganisms Associated with Phytophthora colocasiae Causal Agent of Taro Mildew (Colocasia esculenta (L.) Schott)
    AU  - Asseng Charles Carnot
    AU  - Nanda Djomou Giresse Ledoux
    AU  - Ebongo Lobe Emmanuel
    AU  - Akono Ntonga Patrick
    AU  - Mbida Jean Arthur
    AU  - Ngono Ngane Annie
    AU  - Ambang Zachée
    AU  - Monkam Tchamaha Fabrice
    AU  - Djouokep Léonel Gautier
    Y1  - 2017/10/10
    PY  - 2017
    N1  - https://doi.org/10.11648/j.plant.s.2017050501.15
    DO  - 10.11648/j.plant.s.2017050501.15
    T2  - Plant
    JF  - Plant
    JO  - Plant
    SP  - 33
    EP  - 42
    PB  - Science Publishing Group
    SN  - 2331-0677
    UR  - https://doi.org/10.11648/j.plant.s.2017050501.15
    AB  - Symbiotic pathogenic microorganisms associated with P. colocasiae leading to the manifestation of taro mildew have been identified and studied. The experiment was carried out in an experimental set-up consisting of completely randomized blocks with three treatments (infection with the pure strain, with the unpurified strain and a co-infection with both strains). Isolation and purification of the microorganisms was carried out in the PDA medium and the identification on the analysis of the macroscopic and microscopic morphological characters. The results of the trapping revealed among several a single microorganism which has a high affinity with P. colocasiae, this microorganism named Vorticella is identified as the agent of the amplification of the severity of the downy mildew. Greenhouse studies of taro plants showed that the unpurified strain caused enough damage with 96.67% disease incidence and a severity of 70.18% compared to plants inoculated with pure strain which had an incidence of 40% and a severity of 10.48%. All these results show that P. colocasiae is the causal agent of the mildew of the taro in the infection and the development of the disease; it is accompanied by other microorganisms with which it forms a biofilm. In this biofilm there is a microorganism which plays a very important role in the spread of the disease and it is thanks to it that all parts of the plant are attacked.
    VL  - 5
    IS  - 5-1
    ER  - 

    Copy | Download

  • Sections