Animal and Veterinary Sciences

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Detection of Respiratory Viral Antigens in Cattle Lung Tissues by Direct ELISA

Received: 13 August 2014    Accepted: 28 August 2014    Published: 10 September 2014
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Abstract

Bovine Respiratory Disease is one of the most important diseases with serious financial losses for the cattle industry in worldwide. The aim of this study was to detect the associations between respiratory viruses; bovine herpes virus 1, bovine viral diarrhea virus, bovine respiratory syncytial virus, and Para influenza virus 3 statuses of a herd and bovine respiratory disease occurrence. Present study describes virological distribution of bovine respiratory viruses in non-vaccinated cattle (for mentioned infections) of Central Anatolia, Turkey. A total of 24 lung tissue samples were collected during the December 2012 to January 2013 from cattle that died after manifesting clinical signs of respiratory system. Samples were successfully homogenized. Tissue samples were analyzed for detecting antigens by commercially available direct ELISA kit. BRSV antigens were detected in lung tissues 4 out of 24 tested cattle with a percentage of 16.6%, whereas BHV-1, BVDV and PI-3 were not found. BRSV may be common reason of respiratory diseases in herds. It has been also offered advice about prevention of respiratory viral infection for health planning. In conclusion, existence of BRSV infection is still defined and may play an important role in the respiratoric viral infection of cattle.

DOI 10.11648/j.avs.20140205.13
Published in Animal and Veterinary Sciences (Volume 2, Issue 5, September 2014)
Page(s) 146-149
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This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

BHV-1, BRSV, BVDV, PI-3, ELISA

References
[1] Gillette K.G. and Smith, P.C. (1985). Respiratory syncytial virus infection in transported calves. American Journal of Veterinary Research, 46: 2596-2600.
[2] Muylkens, B., Thiry, J., Kirten, P., Schynts, F. and Thiry, E. (2007). Bovine herpesvirus 1 infection and infectious bovine rhinotracheitis. Veterinary Research, 38: 181-209.
[3] Kampa, J., Stahl, K., Moreno-Lopez, J., Chanlun, A., Aiumlamai, S. and Alenius, S. (2004). BVDV and BHV-1 infections in dairy herds in Northern and Northeastern Thailand. Acta Veterinaria Scandinavica, 45: 181-192.
[4] Tikoo, S.K., Campos, M. and Babiuk, L.A. (1995). Bovine herpesvirus 1 (BHV-1): biology, pathogenesis, and control. Advances in Virus Research, 45: 191-223.
[5] Bowland, S.L. and Shewen, P.E. (2000). Bovine respiratory disease: commercial vaccines currently available in Canada. The Canadian Veterinary Journal, 41: 33-48.
[6] Schwyzer, M. and Ackermann, M. (1996). Molecular virology of ruminant herpesviruses. Veterinary Microbiology, 53: 17-29.
[7] Peterhans, E. and Schweizer, M. (2010). Pestiviruses: How to outmaneuver your hosts. Veterinary Microbiology, 142: 18-25.
[8] Bedekovic, T., Lemo, N., Barbic, L., Cvetnic, Z., Lojkic, I., Benic, M., Cac, Z., Lojkic, M. and Madic, J. (2013). Influence of category, herd size, grazing and management on epidemiology of bovine viral diarrhoea in dairy herds. Acta Veterinaria Brno, 82: 125-130.
[9] Safarpoor Dehkordi, F. (2011). Prevalence study of Bovine viral diarrhea virus by evaluation of antigen capture ELISA and RT-PCR assay in bovine, ovine, caprine, buffalo and camel aborted fetuses in Iran. AMB Express, 1: 32.
[10] Nettleton, P.F. and Entrican, G. (1995). Ruminant pestiviruses. The British Veterinary Journal, 151: 615-642.
[11] Ellis, J., West, K., Knobby, C., Leard, T., Gallo, G., Colon, J. and Fitzgerald, N. (2001). Efficacy of an inactivated respiratory syncytial virus vaccine in calves. Journal of the American Veterinary Medical Association, 218: 1973-1980.
[12] Mink, M.A., Stec, D.S. and Collins, P.L. (1991). Nucleotide sequences of the 3' leader and 5' trailer regions of human respiratory syncytial virus genomic RNA. Virology, 185: 615-624.
[13] Masot, A.J., Gomez, T.C., Tovar, I., Gazquez, A. and Redondo, E. (1993). Location of bovine respiratory syncytial virus antigens in the lung of experimentally infected lambs: comparative study using indirect fluorescent antibody test, avidine biotin peroxidase complex and transmission electron microscopy. European Journal of Histochemistry, 37: 75-82.
[14] Solis-Calderon, J.J., Segura-Correa, V.M., Segura-Correa, J.C. and Alvarado-Islas, A. (2003). Seroprevalence of and risk factors for infectious bovine rhinotracheitis in beef cattle herds of Yucatan, Mexico. Preventive Veterinary Medicine, 57: 199-208.
[15] Banerji, A., Bell, A., Mills, E.L., McDonald, J., Subbarao, K., Stark, G., Eynon, N. and Loo, V.G. (2001). Lower respiratory tract infections in Inuit infants on Baffin Island. Canadian Medical Association Journal, 164: 1847-1850.
[16] Valentova, V., Kovarcik, K. and Psikal, I. (2003). Detection of Bovine respiratory syncytial virus in cell cultures by nRT-PCR and use of the method for virus identification in clinical samples. Acta Veterinaria Brno, 72: 115.
[17] Sakhaee, E.A., Khalili, M. and Kazeminia, S. (2009). Serological study of bovine viral respiratory diseases in dairy cattle of Kerman (Iran). The Iranian Journal of Veterinary Research, 10: 49-53.
[18] Houe, H. (2003). Economic impact of BVDV infection in dairies. Biologicals, 31: 137-143.
[19] Pardon, B., De Bleecker, K., Dewulf, J., Callens, J., Boyen, F., Catry, B. and Deprez, P. (2011). Prevalence of respiratory pathogens in diseased, non-vaccinated, routinely medicated veal calves. Veterinary Record, 169: 278.
[20] Alkan, F., Ozkul, A., Bilge-Dagalp, S., Yesilbag, K., Oguzoglu, C., Akca, Y. and Burgu, I. (2000). Virological and serological studies on the role of PI-3 virus, BRSV, BVDV and BHV- 1 on respiratory infections of cattle. I. The detection of etiological agents by direct immunofluorescence technique. Deutsche Tierarztliche Wochenschrift, 107: 193-195.
[21] Yesilbag, K. and Gungor, B. (2008). Seroprevalence of bovine respiratory viruses in North-Western Turkey. Tropical Animal Health and Production, 40: 55-60.
[22] Saber, M.S., Salem, A.A., Mohamed, S.A.H., Hadia, A.H.H., Nawal, A.M., Mossa, A.A. and Fatheia, M.M. (1996). Isolation, identification and seroconversion studies on BRSV in Egypt. Veterinary Medical Journal Giza, 44: 735-747.
[23] Urban-Chmiel, R., Wernicki, A., Grooms, D.L., Barbu, N. I., Rola, J. and Socha, W. (2013). Rapid detection of Bovine respiratory syncytial virus in Poland using a human patient-side diagnostic assay. Transboundary and Emerging Diseases, doi: 10.1111/tbed.12134
[24] Baker, J.C., Werdin, R.E., Ames, T.R., Markham, R.J.F. and Larson, V.L. (1986). Study on the etiologic role of bovine respiratory syncytial virus in pneumonia of dairy calves. Journal of the American Veterinary Medical Association, 189: 66-86.
[25] Van der Poel, W.H., Brand, A., Kramps, J.A. and Van Oirshot, J.T. (1994). Respiratory syncytial virus infections in human beings and in cattle. Journal of Infection, 29: 215-228.
[26] Lokensgard, B.E., Goyal, S.M. and Krueger, D.A. (1992). Comparison of three immunoassays for the rapid detection of bovine respiratory syncytial virus. Microbiologica, 15: 259–264.
[27] Thonur, L., Maley, M., Gilray, J., Crook, T., Laming, E., Turnbull, D., Nath, M. and Willoughby, K. (2012). One-step multiplex real time RT-PCR for the detection of bovine respiratory syncytial virus, bovine herpesvirus 1 and bovine parainfluenza virus 3. BMC Veterinary Research, 8: 37.
[28] Sausker, E.A. and Dyer, N.W. (2002). Seroprevalence of OHV-2, BVDV, BHV-1, and BRSV in ranch-raised bison (Bison bison). Journal of Veterinary Diagnostic Investigation, 14: 68-70.
[29] Yavru, S., Simsek, A., Yapkic, O. and Kale, M. (2005). Serological evaluation of viral infections in bovine respiratory tract. Acta Veterinaria (Beograd), 55: 219-226.
[30] Duman, R., Yavru, S., Kale, M., and Avci, O. (2009). Seroprevalence of viral upper respiratory infections in dairy cattle. Journal of the Faculty of Veterinary Medicine, University of Kafkas, 15: 539-542.
[31] Hodgson, P.D., Aich, A., Manuja, A., Hokamp, H., Roche, F.M., Brinkman, F.S.I., Potter, A., Babiuk, L.A., and Griebel, P.J. (2005). Effect of stress on viral-bacterial synergy in bovine respiratory disease; novel mechanisms to regulate inflammation. Comparative and Functional Genomics, 6: 244-250.
[32] Valarcher, J.F. and Haaglund, S. (2006). Viral respiratory infections in cattle. In: Proceedings of XXIV World Buiatrics Congress, Nice, France, 2006.
Author Information
  • Department of Virology, Faculty of Veterinary Medicine, University of Selcuk, 42075, Konya, Turkey

  • Department of Virology, Faculty of Veterinary Medicine, University of Selcuk, 42075, Konya, Turkey

  • Virology Laboratory, Veterinary Control Institute, 42080, Konya, Turkey

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  • APA Style

    Oguzhan Avci, Sibel Yavru, Mehmet Ekik. (2014). Detection of Respiratory Viral Antigens in Cattle Lung Tissues by Direct ELISA. Animal and Veterinary Sciences, 2(5), 146-149. https://doi.org/10.11648/j.avs.20140205.13

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    ACS Style

    Oguzhan Avci; Sibel Yavru; Mehmet Ekik. Detection of Respiratory Viral Antigens in Cattle Lung Tissues by Direct ELISA. Anim. Vet. Sci. 2014, 2(5), 146-149. doi: 10.11648/j.avs.20140205.13

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    AMA Style

    Oguzhan Avci, Sibel Yavru, Mehmet Ekik. Detection of Respiratory Viral Antigens in Cattle Lung Tissues by Direct ELISA. Anim Vet Sci. 2014;2(5):146-149. doi: 10.11648/j.avs.20140205.13

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  • @article{10.11648/j.avs.20140205.13,
      author = {Oguzhan Avci and Sibel Yavru and Mehmet Ekik},
      title = {Detection of Respiratory Viral Antigens in Cattle Lung Tissues by Direct ELISA},
      journal = {Animal and Veterinary Sciences},
      volume = {2},
      number = {5},
      pages = {146-149},
      doi = {10.11648/j.avs.20140205.13},
      url = {https://doi.org/10.11648/j.avs.20140205.13},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.avs.20140205.13},
      abstract = {Bovine Respiratory Disease is one of the most important diseases with serious financial losses for the cattle industry in worldwide. The aim of this study was to detect the associations between respiratory viruses; bovine herpes virus 1, bovine viral diarrhea virus, bovine respiratory syncytial virus, and Para influenza virus 3 statuses of a herd and bovine respiratory disease occurrence. Present study describes virological distribution of bovine respiratory viruses in non-vaccinated cattle (for mentioned infections) of Central Anatolia, Turkey. A total of 24 lung tissue samples were collected during the December 2012 to January 2013 from cattle that died after manifesting clinical signs of respiratory system. Samples were successfully homogenized. Tissue samples were analyzed for detecting antigens by commercially available direct ELISA kit. BRSV antigens were detected in lung tissues 4 out of 24 tested cattle with a percentage of 16.6%, whereas BHV-1, BVDV and PI-3 were not found. BRSV may be common reason of respiratory diseases in herds. It has been also offered advice about prevention of respiratory viral infection for health planning. In conclusion, existence of BRSV infection is still defined and may play an important role in the respiratoric viral infection of cattle.},
     year = {2014}
    }
    

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    T1  - Detection of Respiratory Viral Antigens in Cattle Lung Tissues by Direct ELISA
    AU  - Oguzhan Avci
    AU  - Sibel Yavru
    AU  - Mehmet Ekik
    Y1  - 2014/09/10
    PY  - 2014
    N1  - https://doi.org/10.11648/j.avs.20140205.13
    DO  - 10.11648/j.avs.20140205.13
    T2  - Animal and Veterinary Sciences
    JF  - Animal and Veterinary Sciences
    JO  - Animal and Veterinary Sciences
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    EP  - 149
    PB  - Science Publishing Group
    SN  - 2328-5850
    UR  - https://doi.org/10.11648/j.avs.20140205.13
    AB  - Bovine Respiratory Disease is one of the most important diseases with serious financial losses for the cattle industry in worldwide. The aim of this study was to detect the associations between respiratory viruses; bovine herpes virus 1, bovine viral diarrhea virus, bovine respiratory syncytial virus, and Para influenza virus 3 statuses of a herd and bovine respiratory disease occurrence. Present study describes virological distribution of bovine respiratory viruses in non-vaccinated cattle (for mentioned infections) of Central Anatolia, Turkey. A total of 24 lung tissue samples were collected during the December 2012 to January 2013 from cattle that died after manifesting clinical signs of respiratory system. Samples were successfully homogenized. Tissue samples were analyzed for detecting antigens by commercially available direct ELISA kit. BRSV antigens were detected in lung tissues 4 out of 24 tested cattle with a percentage of 16.6%, whereas BHV-1, BVDV and PI-3 were not found. BRSV may be common reason of respiratory diseases in herds. It has been also offered advice about prevention of respiratory viral infection for health planning. In conclusion, existence of BRSV infection is still defined and may play an important role in the respiratoric viral infection of cattle.
    VL  - 2
    IS  - 5
    ER  - 

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