Food-borne salmonellosis is the most prevalent disease and major source of Salmonella spp in humans and its detection particularly in developing countries is quite cumbersome and time consuming. Molecular methods for its detection as well as the genotypic diversity of some of the genes responsible for Salmonella virulence are necessary. The aim of the study was to screen for Salmonella spp using the 16S rRNA, to determine whether the invA gene is specific for Salmonella detection as well as virulence genotyping of some genes present in Salmonella spp (invA, sitC and spvA, spvB and spvC) from food samples in Lagos, Nigeria. All 76 isolates tested positive for 16S rRNA gene while 53 (69.7%) were positive for salm3 and salm4 (389 bp) gene. PCR analysis of the invA gene (284bp) showed that 73 (96.1%) were positive, 38 (50%) of the isolates were positive for sitC gene while none were positive for spvA and spvB and with the multiplex –PCR of invA/spvC gene 25 (33%) were positive for invA (244 bp) gene and none positive for spvC gene. The use of invA gene for Salmonella detection in our food samples is recommended however for most of our isolates the virulence genes were not detected.
| DOI | 10.11648/j.avs.20150301.15 |
| Published in | Animal and Veterinary Sciences (Volume 3, Issue 1, January 2015) |
| Page(s) | 22-27 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
Detection, Food Samples, PCR, Salmonella, Virulence Genes
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APA Style
Stella Ifeanyi Smith, Muinah Adenike Fowora, Adedamilola Atiba, Joseph Anejo-Okopi, Tina Fingesi, et al. (2015). Molecular Detection of Some Virulence Genes in Salmonella Spp Isolated from Food Samples in Lagos, Nigeria. Animal and Veterinary Sciences, 3(1), 22-27. https://doi.org/10.11648/j.avs.20150301.15
ACS Style
Stella Ifeanyi Smith; Muinah Adenike Fowora; Adedamilola Atiba; Joseph Anejo-Okopi; Tina Fingesi, et al. Molecular Detection of Some Virulence Genes in Salmonella Spp Isolated from Food Samples in Lagos, Nigeria. Anim. Vet. Sci. 2015, 3(1), 22-27. doi: 10.11648/j.avs.20150301.15
AMA Style
Stella Ifeanyi Smith, Muinah Adenike Fowora, Adedamilola Atiba, Joseph Anejo-Okopi, Tina Fingesi, et al. Molecular Detection of Some Virulence Genes in Salmonella Spp Isolated from Food Samples in Lagos, Nigeria. Anim Vet Sci. 2015;3(1):22-27. doi: 10.11648/j.avs.20150301.15
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author = {Stella Ifeanyi Smith and Muinah Adenike Fowora and Adedamilola Atiba and Joseph Anejo-Okopi and Tina Fingesi and Mary Ehi Adamu and Emmanuel Adedayo Omonigbehin and Margaret Iteun Ugo-Ijeh and Moses Bamidele and Peter Odeigah},
title = {Molecular Detection of Some Virulence Genes in Salmonella Spp Isolated from Food Samples in Lagos, Nigeria},
journal = {Animal and Veterinary Sciences},
volume = {3},
number = {1},
pages = {22-27},
doi = {10.11648/j.avs.20150301.15},
url = {https://doi.org/10.11648/j.avs.20150301.15},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.avs.20150301.15},
abstract = {Food-borne salmonellosis is the most prevalent disease and major source of Salmonella spp in humans and its detection particularly in developing countries is quite cumbersome and time consuming. Molecular methods for its detection as well as the genotypic diversity of some of the genes responsible for Salmonella virulence are necessary. The aim of the study was to screen for Salmonella spp using the 16S rRNA, to determine whether the invA gene is specific for Salmonella detection as well as virulence genotyping of some genes present in Salmonella spp (invA, sitC and spvA, spvB and spvC) from food samples in Lagos, Nigeria. All 76 isolates tested positive for 16S rRNA gene while 53 (69.7%) were positive for salm3 and salm4 (389 bp) gene. PCR analysis of the invA gene (284bp) showed that 73 (96.1%) were positive, 38 (50%) of the isolates were positive for sitC gene while none were positive for spvA and spvB and with the multiplex –PCR of invA/spvC gene 25 (33%) were positive for invA (244 bp) gene and none positive for spvC gene. The use of invA gene for Salmonella detection in our food samples is recommended however for most of our isolates the virulence genes were not detected.},
year = {2015}
}
TY - JOUR T1 - Molecular Detection of Some Virulence Genes in Salmonella Spp Isolated from Food Samples in Lagos, Nigeria AU - Stella Ifeanyi Smith AU - Muinah Adenike Fowora AU - Adedamilola Atiba AU - Joseph Anejo-Okopi AU - Tina Fingesi AU - Mary Ehi Adamu AU - Emmanuel Adedayo Omonigbehin AU - Margaret Iteun Ugo-Ijeh AU - Moses Bamidele AU - Peter Odeigah Y1 - 2015/01/28 PY - 2015 N1 - https://doi.org/10.11648/j.avs.20150301.15 DO - 10.11648/j.avs.20150301.15 T2 - Animal and Veterinary Sciences JF - Animal and Veterinary Sciences JO - Animal and Veterinary Sciences SP - 22 EP - 27 PB - Science Publishing Group SN - 2328-5850 UR - https://doi.org/10.11648/j.avs.20150301.15 AB - Food-borne salmonellosis is the most prevalent disease and major source of Salmonella spp in humans and its detection particularly in developing countries is quite cumbersome and time consuming. Molecular methods for its detection as well as the genotypic diversity of some of the genes responsible for Salmonella virulence are necessary. The aim of the study was to screen for Salmonella spp using the 16S rRNA, to determine whether the invA gene is specific for Salmonella detection as well as virulence genotyping of some genes present in Salmonella spp (invA, sitC and spvA, spvB and spvC) from food samples in Lagos, Nigeria. All 76 isolates tested positive for 16S rRNA gene while 53 (69.7%) were positive for salm3 and salm4 (389 bp) gene. PCR analysis of the invA gene (284bp) showed that 73 (96.1%) were positive, 38 (50%) of the isolates were positive for sitC gene while none were positive for spvA and spvB and with the multiplex –PCR of invA/spvC gene 25 (33%) were positive for invA (244 bp) gene and none positive for spvC gene. The use of invA gene for Salmonella detection in our food samples is recommended however for most of our isolates the virulence genes were not detected. VL - 3 IS - 1 ER -
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