Animal and Veterinary Sciences

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Investigation of Rotavirus Infection in Calves with Diarrhea in Northeast Turkey

Received: 08 December 2015    Accepted: 16 December 2015    Published: 30 December 2015
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Abstract

Rotavirus is the most common cause of severe diarrhea in newborn calves. In this study, it was aimed to investigate frequency of Rotavirus infections in calves in Kars region in Northeast Turkey by feces sampling 112 diarrheic calves of 0-6 months old. Feces samples were tested for Rotavirus antigen by Enzyme Linked Immunosorbent Assay (ELISA). Of the 112 feces samples examined by ELISA 10 samples were identified as positive (8.92%). At the molecular stage, reverse transcription polymerase chain reaction (RT-PCR) technique was used to determine the presence of Rotavirus nucleic acid by using specific generic primers for VP6 genetic regions in feces samples. The 10 samples that were rotavirus antigen positive by ELISA were also positive on RT-PCR technique. The proportion of positivity in feces samples suggested that rotavirus infections are common in the Kars region in Northeast Turkey.

DOI 10.11648/j.avs.20160401.11
Published in Animal and Veterinary Sciences (Volume 4, Issue 1, January 2016)
Page(s) 1-4
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This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Calves, Diarrhea, ELISA, RT-PCR

References
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Author Information
  • Department of Virology, Faculty of Veterinary Medicine, University of Kafkas, Kars, Turkey

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    Volkan Yilmaz. (2015). Investigation of Rotavirus Infection in Calves with Diarrhea in Northeast Turkey. Animal and Veterinary Sciences, 4(1), 1-4. https://doi.org/10.11648/j.avs.20160401.11

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    Volkan Yilmaz. Investigation of Rotavirus Infection in Calves with Diarrhea in Northeast Turkey. Anim. Vet. Sci. 2015, 4(1), 1-4. doi: 10.11648/j.avs.20160401.11

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    AMA Style

    Volkan Yilmaz. Investigation of Rotavirus Infection in Calves with Diarrhea in Northeast Turkey. Anim Vet Sci. 2015;4(1):1-4. doi: 10.11648/j.avs.20160401.11

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  • @article{10.11648/j.avs.20160401.11,
      author = {Volkan Yilmaz},
      title = {Investigation of Rotavirus Infection in Calves with Diarrhea in Northeast Turkey},
      journal = {Animal and Veterinary Sciences},
      volume = {4},
      number = {1},
      pages = {1-4},
      doi = {10.11648/j.avs.20160401.11},
      url = {https://doi.org/10.11648/j.avs.20160401.11},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.avs.20160401.11},
      abstract = {Rotavirus is the most common cause of severe diarrhea in newborn calves. In this study, it was aimed to investigate frequency of Rotavirus infections in calves in Kars region in Northeast Turkey by feces sampling 112 diarrheic calves of 0-6 months old. Feces samples were tested for Rotavirus antigen by Enzyme Linked Immunosorbent Assay (ELISA). Of the 112 feces samples examined by ELISA 10 samples were identified as positive (8.92%). At the molecular stage, reverse transcription polymerase chain reaction (RT-PCR) technique was used to determine the presence of Rotavirus nucleic acid by using specific generic primers for VP6 genetic regions in feces samples. The 10 samples that were rotavirus antigen positive by ELISA were also positive on RT-PCR technique. The proportion of positivity in feces samples suggested that rotavirus infections are common in the Kars region in Northeast Turkey.},
     year = {2015}
    }
    

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    T1  - Investigation of Rotavirus Infection in Calves with Diarrhea in Northeast Turkey
    AU  - Volkan Yilmaz
    Y1  - 2015/12/30
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    PB  - Science Publishing Group
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    UR  - https://doi.org/10.11648/j.avs.20160401.11
    AB  - Rotavirus is the most common cause of severe diarrhea in newborn calves. In this study, it was aimed to investigate frequency of Rotavirus infections in calves in Kars region in Northeast Turkey by feces sampling 112 diarrheic calves of 0-6 months old. Feces samples were tested for Rotavirus antigen by Enzyme Linked Immunosorbent Assay (ELISA). Of the 112 feces samples examined by ELISA 10 samples were identified as positive (8.92%). At the molecular stage, reverse transcription polymerase chain reaction (RT-PCR) technique was used to determine the presence of Rotavirus nucleic acid by using specific generic primers for VP6 genetic regions in feces samples. The 10 samples that were rotavirus antigen positive by ELISA were also positive on RT-PCR technique. The proportion of positivity in feces samples suggested that rotavirus infections are common in the Kars region in Northeast Turkey.
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