Measurement of Biomarkers Troponin I, NT Pro BNP and ANP Between Horses with Signs of Respiratory Disease and Apparently Healthy Group

Fereydon Rezazadeh; Neda Hosseinzadeh; Nahid Ghaderian

doi:doi:10.11648/j.avs.20160406.11

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Measurement of Biomarkers Troponin I, NT Pro BNP and ANP Between Horses with Signs of Respiratory Disease and Apparently Healthy Group

Fereydon Rezazadeh, Neda Hosseinzadeh, Nahid Ghaderian

Published in Animal and Veterinary Sciences (Volume 4, Issue 6)

Received: 1 October 2016 Accepted: 11 October 2016 Published: 3 November 2016

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Abstract

Biomarkers are biological gnomons of processes or events occurring within the body. Biomarkers can be sub-divided into leakage enzymes (Cardiac troponin I) and functional markers (Natriuretic peptides). Some studies were represented that these biomarkers may be changes in respiratory disorders. Therefore, present study is performed to evaluate serumic levels of cardiac biomarkers between horses with respiratory disease and apparently healthy horses. Initially 10 horses with manifestations of respiratory disease (include increasing of respiratory and heart rate, coughing, mucopurulent and purulent nasal discharge, mucosal hyperemia, increased CRT) and 8 apparently healthy horses underwent a clinical examination. Age, gender, breed and color of individual horses were recorded. CTnI and NT pro BNP were measured by chemiluminescence immunoassay but ANP was measured by ELISA kit in the serum of blood. Electrocardiogram (ECG) has been recorded in base-apex lead in the stall. The mean ages were of 2.1±00.8 and 1.7±0.8 in apparently healthy horses and patient groups, respectively. The mean value level of all cardiac biomarkers (cTnI, ANP and NT pro BNP) in patient group (0.008±0.002 ng/ml, 280.48±79.51 ng/L and 20.34±0.68 pg/ml) was higher than apparently healthy group (0.007±0.002 ng/ml, 206.92±23.01 ng/L and 20.00±0.00 pg/ml). But, the statistical differences between the groups were not significant (P>0.05). No significant differences were found in waveforms of electrocardiograms between two groups.

Published in	Animal and Veterinary Sciences (Volume 4, Issue 6)
DOI	10.11648/j.avs.20160406.11
Page(s)	86-92
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Horse, Biomarker, cTnI, NT pro BNP, ANP

References

[1]	S. A. Jesty. Cardiac biomarkers in equine medicine. The Veterinary Journal. Vol. 192, 2012, pp. 131-132.
[2]	F. Apple. Tissue speciﬁcity of cardiac troponin I, cardiac troponin T and creatine kinase-MB. Clinica Chimica Acta. Vol. 284, 1999, pp. 151-159.
[3]	J. E. Adams, G. S. Bodor, V. G. Davila-Roman, J. A. Delmez, F. S. Apple, J. H. Ladenson, A. S. Jaffe. Cardiac troponin I. A marker with high speciﬁcity for cardiac injury. Circulation. Vol. 88, 1993, pp. 101-106.
[4]	G. O. Evans. Biochemical assessment of cardiac function and damage in animal species. A review of the current approach of the academic, governmental and industrial institutions represented by the Animal Clinical Chemistry Association. Journal of Applied Toxicology. Vol. 11, 1991, pp. 15-21.
[5]	T. H. Lee, L. Goldman. Serum enzyme assays in the diagnosis of acute myocardial infarction. Recommendations based on a quantitative analysis. Annals of Internal Medicine. Vol. 105, 1986, pp. 221-233.
[6]	K. Nakai, K. Nakai, Y. Nagane, W. Obara, M. Sato, K. Ohi, N. Matsumoto, N. Takanashi, C. Itoh. Serum levels of cardiac troponin I and other marker proteins in patients with chronic renal failure. Clinical and Experimental Nephrology. Vol. 8, 2004, pp. 43-47.
[7]	S. A. Argiroudis, J. E. Kent, D. J. Blackmore. Observations on the isoenzymes of creatine kinase in equine serum and tissues. Search Results. Equine Veterinary Journal. Vol. 14, 1982, pp. 317-321.
[8]	R. M. Radcliffe, B. R. Buchanan, V. L. Cook, T. J. Divers. The clinical value of whole blood point-of-care biomarkers in large animal emergency and critical care medicine. Journal of Veterinary Emergency and Critical Care. Vol. 25, 2015, pp. 138-151. doi: 10.1111/vec.12276.
[9]	J. Mair, N. Genser, D. Morandell, J. Maier, P. Mair, P. Lechleitner, C. Calzolari, C. Larue, E. Ambach, F. Dienstl, B. Pau, B. Puschendorf. Cardiac troponin I in the diagnosis of myocardial injury and infarction. Clinica Chimica Acta. Vol. 245, 1996, pp. 19-38.
[10]	V. Ricchiuti, S. W. Sharkey, M. M. Murakami, E. M. Voss, F. S. Apple. Cardiac troponin I and T alterations in dog hearts with myocardial infarction: correlation with infarct size. American Journal of Clinical Pathology. Vol. 110, 1998, pp. 241-247.
[11]	K. E. Schober, B. Kirbach, G. Oechtering. Noninvasive assessment of myocardial cell injury in dogs with suspected cardiac contusion. Journal of Veterinary Cardiology. Vol.1, 1999, pp. 17-25.
[12]	P. J. O’Brien, Y. Landt, J. H. Ladenson. Differential reactivity of cardiac and skeletalmuscle fromvarious species in a cardiac troponin I immunoassay. Clinical Chemistry. Vol. 43, 1997, pp. 2333-2338.
[13]	M. Rishniw and K. W. Simpson. Cloning and sequencing of equine cardiac troponin I and conﬁrmation of its usefulness as a target analyte for commercial troponin I analyzers. Journal of Veterinary Diagnostic Investigation. Vol. 17, 2005, pp. 582-584.
[14]	M. Serra, S. Papakonstantinou, M. Adamcova, P. J. O'Brien. Veterinary and toxicological applications for the detection of cardiac injury using cardiac troponin. The Veterinary Journal. Vol. 185, 2010, pp. 50-57.
[15]	S. A. Jesty, M. S. Kraus, A. R. Gelzer. Measurement of plasma cardiac troponin I concentration by use of a point-of-care analyzer in clinically normal horses and horses with experimentally induced cardiac disease. American Journal of Veterinary Research. Vol. 71, 2010, pp. 55-59.
[16]	R. Prosek, D. D. Sisson, M. A. Oyama, P. F. Solter. Distinguishing cardiac and noncardiac dyspnea in 48 dogs using plasma atrial natriuretic factor, B-type natriuretic factor, endothelin, and cardiac troponin-I. Journal of Veterinary Internal Medicine. Vol. 21, 2007, pp. 238-242.
[17]	T. M. Zimmering, F. Meneses, I. J. Nolte, D. Simon. Measurement of N-terminal proatrial natriuretic peptide in plasma of cats with and without cardiomyopathy. American Journal of Veterinary Research. Vol. 70, 2009, pp. 216-222.
[18]	V. Chetboul, F. Serres, R. Tissier, H. P. Lefebvre, C. C. Sampedrano, V. Gouni,, L. Poujol,, G. Hawa, J. L. Pouchelon. Association of plasma N-terminal Pro-B-type natriuretic peptide concentration with mitral regurgitation severity and outcome in dogs with asymptomatic degenerative mitral valve disease. Journal of Veterinary Internal Medicine. Vol. 23, 2009, pp. 984-994.
[19]	D. S. Trachsel, B. Grenacher, M. A. Weishaupt, C. C. Schwarzwald. Plasma atrial natriuretic peptide concentrations in horses with heart disease: A pilot study. Vet. J. Vol. 192, 2012, pp. 166-170.
[20]	K. E. Schober, C. Cornand, B. Kirbach. Serum cardiac troponin I and cardiac troponin T concentrations in dogs with gastric dilatation-volvulus. J. Am. Vet. Med. Assoc. Vol. 221, 2002, pp. 381-388.
[21]	M. Rishniw, S. C. Barr, K. W. Simpson. Cloning and sequencing of the canine and feline cardiac troponin I genes. American Journal of Veterinary Research. Vol. 65, 2004, pp. 53-58.
[22]	W. Phillips, S. Giguère, R. P. Franklin, J. Hernandez, D. Adin, J. Peloso. Cardiac troponin I in pastured and race-training Thoroughbred horses. Journal of Veterinary Internal Medicine. Vol.17, 2003, pp. 597-599.
[23]	A. Hassanpour. A study on the serumic levels of cardiac troponin and some enzymes in horses with strangles. Indian journal of fundamental and applied life sciences. Vol. 4, 2014, pp. 7-9.
[24]	A. M. Sage, S. Valberg, D. W. Hayden. Echocardiography in a horse with corpulmonale from recurrent airway obstruction. Journal of Veterinary Internal Medicine. Vol. 20, 2006, pp. 694-696.
[25]	C. J. Cornelisse, H. C. Schott, N. B. Olivier, T.P. Mullaney, A. Koller, D. V. Wilson, F. J. Derksen. Concentration of cardiac troponin I in a horse with a ruptured aortic regurgitation jet lesion and ventricular tachycardia. Journal of the American Veterinary Medical Association. Vol. 217, 2000, pp. 231-235.
[26]	N. Van Der Vekens, A. Decloedt, S. Ven, D. De Clercq, G. Van Loon. Cardiac troponin I as compared to troponin T for the detection of myocardial damage in horses. Journal of Veterinary Internal Medicine. Vol. 29, 2015, pp. 348-54. doi: 10.1111/jvim.12530.
[27]	K. M. Ver Elst, H. D. Spapen, D. N. Nguyen. Cardiac troponins I and T are biological markers of left ventricular dysfunction in septic shock. Clinical Chemistry. Vol. 46, 2000, pp. 650-657.
[28]	C. J. Fernandes, N. Akamine, E. Knobel. Cardiac troponin: a new serum marker of myocardial injury in sepsis. Intensive Care Medicine. Vol. 25, 1999, pp. 1165-1168.
[29]	L. B. Yap, D. Mukerjee, P. M. Timms, H. Ashrafian, J. G. Coghlan. Natriuretic peptides, respiratory disease, and the right heart. CHEST. Vol. 126, 2004, pp. 1330-1336.
[30]	G. E. Woodard, J. A. Rosado. Recent advances in natriuretic peptide research. Journal of Cellular and Molecular Medicine. Vol. 11, 2007, pp. 1263-1271.
[31]	G. M. Felker, J. W. Petersen, D. B. Mark. Natriuretic peptides in the diagnosis and management of heart failure. Canadian Medical Association Journal. Vol. 175, 2006, pp. 611-617.
[32]	N. Nagaya, T. Nishikimi, M. Uematsu. Plasma brain natriuretic peptide as a prognostic indicator in patients with primary pulmonary hypertension. Circulation. Vol.102, 2000, pp. 865-870.
[33]	F. Holguin, E. Folch, S. C. Redd. Comorbidity and mortality in COPD-related hospitalizations in the United States, 1979-2001. CHEST. Vol. 128, 2005, pp. 2005-2011.
[34]	D. D. Sin, N. R. Anthonisen, J. B. Soriano. Mortality in COPD: role of comorbidities. European Respiratory Society. Vol. 28, 2006, pp. 1245-1257.
[35]	A. Chaouat, R. Naeije, E. Weitzenblum. Pulmonary hypertension in COPD. European Respiratory Society. Vol. 32, 2008, pp. 1371-1385.
[36]	Y. Saito. Roles of atrial natriuretic peptide and its therapeutic use. Journal of Cardiology. Vol. 56, 2010, pp. 262-270.
[37]	H. Gehlen, T. Sundermann, K. Rohn, P. R. Stadler. Plasma atrial natriuretic peptide concentration in warm-blood horses with heart valve regurgitations. Journal of Veterinary Cardiology. Vol. 9, 2007, pp. 99-101.
[38]	D. S. Trachsel, B. Grenacher, M. A. Weishaupt, C. C. Schwarzwald. Plasma atrial natriuretic peptide concentrations in horses with heart disease: A pilot study. The Veterinary Journal. 2011, pp. 1-5.
[39]	M. Bando, Y. Ishii, Y. Sugiyama. Elevated plasma brain natriuretic peptide levels in chronic respiratory failure with corpulmonale. Respiratory Medicine. Vol. 93, 1999, pp. 507-514.
[40]	I. I. Tulevski, M. Groenink, E. E. Van Der Wall. Increased brain and atrial natriuretic peptides in patients with chronic right ventricular pressure overload: correlation between plasma neurohormones and right ventricular dysfunction. Heart. Vol. 86, 2001, pp. 27-30.

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APA Style

Fereydon Rezazadeh, Neda Hosseinzadeh, Nahid Ghaderian. (2016). Measurement of Biomarkers Troponin I, NT Pro BNP and ANP Between Horses with Signs of Respiratory Disease and Apparently Healthy Group. Animal and Veterinary Sciences, 4(6), 86-92. https://doi.org/10.11648/j.avs.20160406.11

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ACS Style

Fereydon Rezazadeh; Neda Hosseinzadeh; Nahid Ghaderian. Measurement of Biomarkers Troponin I, NT Pro BNP and ANP Between Horses with Signs of Respiratory Disease and Apparently Healthy Group. Anim. Vet. Sci. 2016, 4(6), 86-92. doi: 10.11648/j.avs.20160406.11

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AMA Style

Fereydon Rezazadeh, Neda Hosseinzadeh, Nahid Ghaderian. Measurement of Biomarkers Troponin I, NT Pro BNP and ANP Between Horses with Signs of Respiratory Disease and Apparently Healthy Group. Anim Vet Sci. 2016;4(6):86-92. doi: 10.11648/j.avs.20160406.11

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@article{10.11648/j.avs.20160406.11,
  author = {Fereydon Rezazadeh and Neda Hosseinzadeh and Nahid Ghaderian},
  title = {Measurement of Biomarkers Troponin I, NT Pro BNP and ANP Between Horses with Signs of Respiratory Disease and Apparently Healthy Group},
  journal = {Animal and Veterinary Sciences},
  volume = {4},
  number = {6},
  pages = {86-92},
  doi = {10.11648/j.avs.20160406.11},
  url = {https://doi.org/10.11648/j.avs.20160406.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.avs.20160406.11},
  abstract = {Biomarkers are biological gnomons of processes or events occurring within the body. Biomarkers can be sub-divided into leakage enzymes (Cardiac troponin I) and functional markers (Natriuretic peptides). Some studies were represented that these biomarkers may be changes in respiratory disorders. Therefore, present study is performed to evaluate serumic levels of cardiac biomarkers between horses with respiratory disease and apparently healthy horses. Initially 10 horses with manifestations of respiratory disease (include increasing of respiratory and heart rate, coughing, mucopurulent and purulent nasal discharge, mucosal hyperemia, increased CRT) and 8 apparently healthy horses underwent a clinical examination. Age, gender, breed and color of individual horses were recorded. CTnI and NT pro BNP were measured by chemiluminescence immunoassay but ANP was measured by ELISA kit in the serum of blood. Electrocardiogram (ECG) has been recorded in base-apex lead in the stall. The mean ages were of 2.1±00.8 and 1.7±0.8 in apparently healthy horses and patient groups, respectively. The mean value level of all cardiac biomarkers (cTnI, ANP and NT pro BNP) in patient group (0.008±0.002 ng/ml, 280.48±79.51 ng/L and 20.34±0.68 pg/ml) was higher than apparently healthy group (0.007±0.002 ng/ml, 206.92±23.01 ng/L and 20.00±0.00 pg/ml). But, the statistical differences between the groups were not significant (P>0.05). No significant differences were found in waveforms of electrocardiograms between two groups.},
 year = {2016}
}

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TY - JOUR
T1 - Measurement of Biomarkers Troponin I, NT Pro BNP and ANP Between Horses with Signs of Respiratory Disease and Apparently Healthy Group
AU - Fereydon Rezazadeh
AU - Neda Hosseinzadeh
AU - Nahid Ghaderian
Y1 - 2016/11/03
PY - 2016
N1 - https://doi.org/10.11648/j.avs.20160406.11
DO - 10.11648/j.avs.20160406.11
T2 - Animal and Veterinary Sciences
JF - Animal and Veterinary Sciences
JO - Animal and Veterinary Sciences
SP - 86
EP - 92
PB - Science Publishing Group
SN - 2328-5850
UR - https://doi.org/10.11648/j.avs.20160406.11
AB - Biomarkers are biological gnomons of processes or events occurring within the body. Biomarkers can be sub-divided into leakage enzymes (Cardiac troponin I) and functional markers (Natriuretic peptides). Some studies were represented that these biomarkers may be changes in respiratory disorders. Therefore, present study is performed to evaluate serumic levels of cardiac biomarkers between horses with respiratory disease and apparently healthy horses. Initially 10 horses with manifestations of respiratory disease (include increasing of respiratory and heart rate, coughing, mucopurulent and purulent nasal discharge, mucosal hyperemia, increased CRT) and 8 apparently healthy horses underwent a clinical examination. Age, gender, breed and color of individual horses were recorded. CTnI and NT pro BNP were measured by chemiluminescence immunoassay but ANP was measured by ELISA kit in the serum of blood. Electrocardiogram (ECG) has been recorded in base-apex lead in the stall. The mean ages were of 2.1±00.8 and 1.7±0.8 in apparently healthy horses and patient groups, respectively. The mean value level of all cardiac biomarkers (cTnI, ANP and NT pro BNP) in patient group (0.008±0.002 ng/ml, 280.48±79.51 ng/L and 20.34±0.68 pg/ml) was higher than apparently healthy group (0.007±0.002 ng/ml, 206.92±23.01 ng/L and 20.00±0.00 pg/ml). But, the statistical differences between the groups were not significant (P>0.05). No significant differences were found in waveforms of electrocardiograms between two groups.
VL - 4
IS - 6
ER -

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Author Information

Fereydon Rezazadeh

Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
Neda Hosseinzadeh

Veterinary Pharmacology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
Nahid Ghaderian

Specialist in Clinical-anatomical Pathology, Nour Medical Laboratory, Tabriz, Iran

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Fereydon Rezazadeh, Neda Hosseinzadeh, Nahid Ghaderian. (2016). Measurement of Biomarkers Troponin I, NT Pro BNP and ANP Between Horses with Signs of Respiratory Disease and Apparently Healthy Group. Animal and Veterinary Sciences, 4(6), 86-92. https://doi.org/10.11648/j.avs.20160406.11

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ACS Style

Fereydon Rezazadeh; Neda Hosseinzadeh; Nahid Ghaderian. Measurement of Biomarkers Troponin I, NT Pro BNP and ANP Between Horses with Signs of Respiratory Disease and Apparently Healthy Group. Anim. Vet. Sci. 2016, 4(6), 86-92. doi: 10.11648/j.avs.20160406.11

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AMA Style

Fereydon Rezazadeh, Neda Hosseinzadeh, Nahid Ghaderian. Measurement of Biomarkers Troponin I, NT Pro BNP and ANP Between Horses with Signs of Respiratory Disease and Apparently Healthy Group. Anim Vet Sci. 2016;4(6):86-92. doi: 10.11648/j.avs.20160406.11

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@article{10.11648/j.avs.20160406.11,
  author = {Fereydon Rezazadeh and Neda Hosseinzadeh and Nahid Ghaderian},
  title = {Measurement of Biomarkers Troponin I, NT Pro BNP and ANP Between Horses with Signs of Respiratory Disease and Apparently Healthy Group},
  journal = {Animal and Veterinary Sciences},
  volume = {4},
  number = {6},
  pages = {86-92},
  doi = {10.11648/j.avs.20160406.11},
  url = {https://doi.org/10.11648/j.avs.20160406.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.avs.20160406.11},
  abstract = {Biomarkers are biological gnomons of processes or events occurring within the body. Biomarkers can be sub-divided into leakage enzymes (Cardiac troponin I) and functional markers (Natriuretic peptides). Some studies were represented that these biomarkers may be changes in respiratory disorders. Therefore, present study is performed to evaluate serumic levels of cardiac biomarkers between horses with respiratory disease and apparently healthy horses. Initially 10 horses with manifestations of respiratory disease (include increasing of respiratory and heart rate, coughing, mucopurulent and purulent nasal discharge, mucosal hyperemia, increased CRT) and 8 apparently healthy horses underwent a clinical examination. Age, gender, breed and color of individual horses were recorded. CTnI and NT pro BNP were measured by chemiluminescence immunoassay but ANP was measured by ELISA kit in the serum of blood. Electrocardiogram (ECG) has been recorded in base-apex lead in the stall. The mean ages were of 2.1±00.8 and 1.7±0.8 in apparently healthy horses and patient groups, respectively. The mean value level of all cardiac biomarkers (cTnI, ANP and NT pro BNP) in patient group (0.008±0.002 ng/ml, 280.48±79.51 ng/L and 20.34±0.68 pg/ml) was higher than apparently healthy group (0.007±0.002 ng/ml, 206.92±23.01 ng/L and 20.00±0.00 pg/ml). But, the statistical differences between the groups were not significant (P>0.05). No significant differences were found in waveforms of electrocardiograms between two groups.},
 year = {2016}
}

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