Animal and Veterinary Sciences

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The Prevalence of Melissococcus plutonius by Polymerase Chain Reaction in Kurdistan apiaries, Iran

Received: 12 July 2016    Accepted: 26 July 2016    Published: 14 December 2016
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Abstract

European foulbrood (EFB) is caused by bacterial agents Melissococcus plutonius and is considered of major importance because it can be spread through many bee products including honey, which is internationally widely traded. A polymerase chain reaction (PCR) molecular diagnosis survey for honey bee pathogen EFB was conducted on European honey bee (EHB), Apis mellifera, population sampled from Kurdistan apiaries (n=100). For multiplex PCR amplification of partial 16S rRNA (= SSU rRNA) gene fragments (486 bp), was conducted on 100 samples. Results showed that of 100 samples only 4 samples (4%) were positive for M. plutonius in Kurdistan apiaries. This is the first study to document the occurrence of EFB by PCR assay in Kurdistan apiaries. It seems that surveys are required to characterize the distribution and prevalence of M. plutonius in different regions of Iran.

DOI 10.11648/j.avs.20160406.12
Published in Animal and Veterinary Sciences (Volume 4, Issue 6, November 2016)
Page(s) 93-96
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

M. plutonius, European foulbrood, PCR, Kurdistan apiaries

References
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Author Information
  • Veterinary Research Department, Agricultural and Natural Resources Research and Education Center, AREEO, Sanandaj, Iran

  • Department of Honeybee, Silkworm and Wildlife Research Disease, Razi Vaccine and Serum Research Institute, AREEO, Karaj, Iran

  • Department of Honeybee, Silkworm and Wildlife Research Disease, Razi Vaccine and Serum Research Institute, AREEO, Karaj, Iran

  • Department of Honeybee, Silkworm and Wildlife Research Disease, Razi Vaccine and Serum Research Institute, AREEO, Karaj, Iran

  • Veterinary Research Department, Agricultural and Natural Resources Research and Education Center, AREEO, Sanandaj, Iran

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  • APA Style

    Mohammad Khezri, Mojtaba Moharami, Hussein Modirrousta, Zahra Salahi, Babak Rokhzad. (2016). The Prevalence of Melissococcus plutonius by Polymerase Chain Reaction in Kurdistan apiaries, Iran. Animal and Veterinary Sciences, 4(6), 93-96. https://doi.org/10.11648/j.avs.20160406.12

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    ACS Style

    Mohammad Khezri; Mojtaba Moharami; Hussein Modirrousta; Zahra Salahi; Babak Rokhzad. The Prevalence of Melissococcus plutonius by Polymerase Chain Reaction in Kurdistan apiaries, Iran. Anim. Vet. Sci. 2016, 4(6), 93-96. doi: 10.11648/j.avs.20160406.12

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    AMA Style

    Mohammad Khezri, Mojtaba Moharami, Hussein Modirrousta, Zahra Salahi, Babak Rokhzad. The Prevalence of Melissococcus plutonius by Polymerase Chain Reaction in Kurdistan apiaries, Iran. Anim Vet Sci. 2016;4(6):93-96. doi: 10.11648/j.avs.20160406.12

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  • @article{10.11648/j.avs.20160406.12,
      author = {Mohammad Khezri and Mojtaba Moharami and Hussein Modirrousta and Zahra Salahi and Babak Rokhzad},
      title = {The Prevalence of Melissococcus plutonius by Polymerase Chain Reaction in Kurdistan apiaries, Iran},
      journal = {Animal and Veterinary Sciences},
      volume = {4},
      number = {6},
      pages = {93-96},
      doi = {10.11648/j.avs.20160406.12},
      url = {https://doi.org/10.11648/j.avs.20160406.12},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.avs.20160406.12},
      abstract = {European foulbrood (EFB) is caused by bacterial agents Melissococcus plutonius and is considered of major importance because it can be spread through many bee products including honey, which is internationally widely traded. A polymerase chain reaction (PCR) molecular diagnosis survey for honey bee pathogen EFB was conducted on European honey bee (EHB), Apis mellifera, population sampled from Kurdistan apiaries (n=100). For multiplex PCR amplification of partial 16S rRNA (= SSU rRNA) gene fragments (486 bp), was conducted on 100 samples. Results showed that of 100 samples only 4 samples (4%) were positive for M. plutonius in Kurdistan apiaries. This is the first study to document the occurrence of EFB by PCR assay in Kurdistan apiaries. It seems that surveys are required to characterize the distribution and prevalence of M. plutonius in different regions of Iran.},
     year = {2016}
    }
    

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  • TY  - JOUR
    T1  - The Prevalence of Melissococcus plutonius by Polymerase Chain Reaction in Kurdistan apiaries, Iran
    AU  - Mohammad Khezri
    AU  - Mojtaba Moharami
    AU  - Hussein Modirrousta
    AU  - Zahra Salahi
    AU  - Babak Rokhzad
    Y1  - 2016/12/14
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    DO  - 10.11648/j.avs.20160406.12
    T2  - Animal and Veterinary Sciences
    JF  - Animal and Veterinary Sciences
    JO  - Animal and Veterinary Sciences
    SP  - 93
    EP  - 96
    PB  - Science Publishing Group
    SN  - 2328-5850
    UR  - https://doi.org/10.11648/j.avs.20160406.12
    AB  - European foulbrood (EFB) is caused by bacterial agents Melissococcus plutonius and is considered of major importance because it can be spread through many bee products including honey, which is internationally widely traded. A polymerase chain reaction (PCR) molecular diagnosis survey for honey bee pathogen EFB was conducted on European honey bee (EHB), Apis mellifera, population sampled from Kurdistan apiaries (n=100). For multiplex PCR amplification of partial 16S rRNA (= SSU rRNA) gene fragments (486 bp), was conducted on 100 samples. Results showed that of 100 samples only 4 samples (4%) were positive for M. plutonius in Kurdistan apiaries. This is the first study to document the occurrence of EFB by PCR assay in Kurdistan apiaries. It seems that surveys are required to characterize the distribution and prevalence of M. plutonius in different regions of Iran.
    VL  - 4
    IS  - 6
    ER  - 

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