Animal and Veterinary Sciences

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Toxin Genotyping of C. perfringens Isolated from Broiler Cases of Necrotic Enteritis

Received: 27 August 2017    Accepted: 20 September 2017    Published: 11 November 2017
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Abstract

Clostridium perfringens organisms have an economic concern in poultry production. The purpose of this study was to investigate Net B and β2 C. perfringens positive isolates in broiler farms and their clinic-pathological effects in broiler chicks. A bacteriological examination of C. perfringens was carried upon 92 Necrotic enteritis (NE) diseased cases and 55 apparently healthy broilers of different ages in Egypt. C. perfringens type A was only recovered (49.7%). cpa gene was detected in 100% of samples with PCR technique. NE diseased cases exhibited both Net B (87.5%) and cpb2 (75%) toxin genes. Experimentally, an intra-gut induction of Net B and β2 C. perfringens toxins were evaluated in chicken models. The hematological studies revealed hemolytic anemia 5 days post infection (p.i) in Net B and β2 inoculated groups (G1&G2). Leucogram revealed neutrophilia and lymphopenia 5 days p.i. A significant increase in ALT, AST, uric acid and creatinine serum levels were recorded in the infected groups at 5th and 12th day p.i. DNA Sequencing for net B gene revealed an amino acid replacement from glutamate into arginine at codon 379 with silent mutation was also detected at nucleotide 1134. Sequencing of both toxin genes were recorded in the gene bank for the first time in Egypt. This study pointed out that C. perfringens Net B toxin, is a new key virulent factor for the development of NE. Further studies of Net B toxiod for vaccine production could minimize the clostridial problems in broiler farms.

DOI 10.11648/j.avs.20170506.13
Published in Animal and Veterinary Sciences (Volume 5, Issue 6, November 2017)
Page(s) 108-120
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

C. perfringens, PCR, NET B, Cpb2, Broiler, Virulance, Enteritis, Sequencing

References
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Author Information
  • Bacteriology Department, Animal Health Research Institute, Ismailia, Egypt

  • Bacteriology Department, Anearobic Unit, Animal Health Research Institute, Dokki, Giza, Egypt

  • Bacteriology Department, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt

  • Clinical Pathology Department, Animal Health Research Institute, Ismailia, Egypt

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    Ghada Abdelaal Ibrahim, Basma Shalaby Mahmoud, Ahmed Mohammed Ammar, Fatma Mohammed Youssef. (2017). Toxin Genotyping of C. perfringens Isolated from Broiler Cases of Necrotic Enteritis. Animal and Veterinary Sciences, 5(6), 108-120. https://doi.org/10.11648/j.avs.20170506.13

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    Ghada Abdelaal Ibrahim; Basma Shalaby Mahmoud; Ahmed Mohammed Ammar; Fatma Mohammed Youssef. Toxin Genotyping of C. perfringens Isolated from Broiler Cases of Necrotic Enteritis. Anim. Vet. Sci. 2017, 5(6), 108-120. doi: 10.11648/j.avs.20170506.13

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    AMA Style

    Ghada Abdelaal Ibrahim, Basma Shalaby Mahmoud, Ahmed Mohammed Ammar, Fatma Mohammed Youssef. Toxin Genotyping of C. perfringens Isolated from Broiler Cases of Necrotic Enteritis. Anim Vet Sci. 2017;5(6):108-120. doi: 10.11648/j.avs.20170506.13

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  • @article{10.11648/j.avs.20170506.13,
      author = {Ghada Abdelaal Ibrahim and Basma Shalaby Mahmoud and Ahmed Mohammed Ammar and Fatma Mohammed Youssef},
      title = {Toxin Genotyping of C. perfringens Isolated from Broiler Cases of Necrotic Enteritis},
      journal = {Animal and Veterinary Sciences},
      volume = {5},
      number = {6},
      pages = {108-120},
      doi = {10.11648/j.avs.20170506.13},
      url = {https://doi.org/10.11648/j.avs.20170506.13},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.avs.20170506.13},
      abstract = {Clostridium perfringens organisms have an economic concern in poultry production. The purpose of this study was to investigate Net B and β2 C. perfringens positive isolates in broiler farms and their clinic-pathological effects in broiler chicks. A bacteriological examination of C. perfringens was carried upon 92 Necrotic enteritis (NE) diseased cases and 55 apparently healthy broilers of different ages in Egypt. C. perfringens type A was only recovered (49.7%). cpa gene was detected in 100% of samples with PCR technique. NE diseased cases exhibited both Net B (87.5%) and cpb2 (75%) toxin genes. Experimentally, an intra-gut induction of Net B and β2 C. perfringens toxins were evaluated in chicken models. The hematological studies revealed hemolytic anemia 5 days post infection (p.i) in Net B and β2 inoculated groups (G1&G2). Leucogram revealed neutrophilia and lymphopenia 5 days p.i. A significant increase in ALT, AST, uric acid and creatinine serum levels were recorded in the infected groups at 5th and 12th day p.i. DNA Sequencing for net B gene revealed an amino acid replacement from glutamate into arginine at codon 379 with silent mutation was also detected at nucleotide 1134. Sequencing of both toxin genes were recorded in the gene bank for the first time in Egypt. This study pointed out that C. perfringens Net B toxin, is a new key virulent factor for the development of NE. Further studies of Net B toxiod for vaccine production could minimize the clostridial problems in broiler farms.},
     year = {2017}
    }
    

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  • TY  - JOUR
    T1  - Toxin Genotyping of C. perfringens Isolated from Broiler Cases of Necrotic Enteritis
    AU  - Ghada Abdelaal Ibrahim
    AU  - Basma Shalaby Mahmoud
    AU  - Ahmed Mohammed Ammar
    AU  - Fatma Mohammed Youssef
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    DO  - 10.11648/j.avs.20170506.13
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    JF  - Animal and Veterinary Sciences
    JO  - Animal and Veterinary Sciences
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    PB  - Science Publishing Group
    SN  - 2328-5850
    UR  - https://doi.org/10.11648/j.avs.20170506.13
    AB  - Clostridium perfringens organisms have an economic concern in poultry production. The purpose of this study was to investigate Net B and β2 C. perfringens positive isolates in broiler farms and their clinic-pathological effects in broiler chicks. A bacteriological examination of C. perfringens was carried upon 92 Necrotic enteritis (NE) diseased cases and 55 apparently healthy broilers of different ages in Egypt. C. perfringens type A was only recovered (49.7%). cpa gene was detected in 100% of samples with PCR technique. NE diseased cases exhibited both Net B (87.5%) and cpb2 (75%) toxin genes. Experimentally, an intra-gut induction of Net B and β2 C. perfringens toxins were evaluated in chicken models. The hematological studies revealed hemolytic anemia 5 days post infection (p.i) in Net B and β2 inoculated groups (G1&G2). Leucogram revealed neutrophilia and lymphopenia 5 days p.i. A significant increase in ALT, AST, uric acid and creatinine serum levels were recorded in the infected groups at 5th and 12th day p.i. DNA Sequencing for net B gene revealed an amino acid replacement from glutamate into arginine at codon 379 with silent mutation was also detected at nucleotide 1134. Sequencing of both toxin genes were recorded in the gene bank for the first time in Egypt. This study pointed out that C. perfringens Net B toxin, is a new key virulent factor for the development of NE. Further studies of Net B toxiod for vaccine production could minimize the clostridial problems in broiler farms.
    VL  - 5
    IS  - 6
    ER  - 

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