Retrospective Study of Porcine Circovirus Type 2a and 2b Between 1999 and 2016 in Taiwan
Porcine circovirus type 2 (PCV2) is one of the major swine viral diseases and caused significant economic loss to pig producers worldwide, including Taiwan. PCV2 has been considered as the causative agent of postweaning multisystemic wasting syndrome (PMWS) as well as other clinical diseases. All these associated syndromes have been categorized as PCV2 associated diseases (PCVAD). The purpose of this study was to investigate the positive rate and genetic shift of two distinct genotypes of PCV2, which include PCV2a and PCV2b, in Taiwanese pig farms. A total of 1094 specimens originating from pigs between years 1999 and 2016 were analysed. The PCV2a and PCV2b sequences were amplified and distinguished using 1oop-mediated isothermal amplification (LAMP). Results showed that 24.8% (272/1094) pigs were PCV2a positive, 67.3% (737/1094) were PCV2b positive. These results also indicated that PCV2a was the predominant virus between 1999 and 2001, and that PCV2b became the most prevalent virus since 2003.
Retrospective Study of Porcine Circovirus Type 2a and 2b Between 1999 and 2016 in Taiwan, Animal and Veterinary Sciences.
Vol. 5, No. 6,
2017, pp. 126-130.
Cheung, A. K., 2003. Transcriptional analysis of porcine circovirus type 2. Virology 305, 168-180.
Saha, D., Lefebvre, D. J., Ooms, K., Huang, L., Delputte, P. L., 2012. Single amino acid mutations in the capsid switch the neutralisation phenotype of porcine circovirus 2. J. Gen. Virol. 93, 1548-1555.
Karuppannan, A. K., Liu, S., Jia, Q., Selvaraj, M., Kwang, J., 2010. Porcine circovirus type 2 ORF3 protein competes with p53 in binding to Pirh2 and mediates the deregulation of p53 homeostasis. Virology 398, 1-11.
Olvera, A., Cortey, M., Segalés, J., 2007. Molecular evolution of porcine circovirus type 2 genomes: phylogeny and clonality. Virology 357, 175-185.
Horlen, K. P., Schneider, P., Anderson, J., 2007. A cluster of farms experiencing severe porcine circovirus associated disease: Clinical features and association with the PCV2b genotype. J. Swine Health Prod. 15, 270-278.
Opriessnig, T., Meng, X. J., Halbur, P. G., 2007. Porcine circovirus type 2 associated disease: update on current terminology, clinical manifestations, pathogenesis, diagnosis, and intervention strategies. J. Vet. Diagn. Invest. 19, 591-615.
Segalés, J., Allan, G. M., Domingo, M., 2005. Porcine circovirus diseases. Animal Health Res. Rev. 6, 119-142.
Nieto, D., Aramouni, M., Grau-Roma, L., Segalés, J., Kekarainen, T., 2011. Dynamics of Torque teno sus virus 1 (TTSuV1) and 2 (TTSuV2) DNA loads in serum of healthy and postweaning multisystemic wasting syndrome (PMWS) affected pigs. Vet. Microbiol. 152, 284-290.
Wang, C., Huang, T. S., Huang, C. C., Tu, C., Jong, M. H., Lin, S. Y., Lai, S. S., 2004. Characterization of porcine circovirus type 2 in Taiwan. J. Vet. Med. Sci. 66, 469-475.
Kim, J., Chung, H. K., Jung, T., Cho, W. S., Choi, C., Chae, C., 2002. Postweaning multisystemic wasting syndrome of pigs in Korea: prevalence, microscopic lesions and coexisting microorganisms. J. Vet. Med. Sci. 64, 57-62.
Dupont, K., Nielsen, E. O., Baekbo, P., Larsen, L. E., 2008. Genomic analysis of PCV2 isolates from Danish archives and a current PMWS case-control study supports a shift in genotypes with time. Vet. Microbiol. 128, 56-64.
Gillespie, J., Opriessnig, T., Meng, X. J., Pelzer, K., Buechner-Maxwell, V., 2009. Porcine circovirus type 2 and porcine circovirus-associated disease. J. Vet. Intern. Med. 23, 1151-1163.
Meng, X. J., 2012. Emerging and Re-emerging Swine Viruses. Transboundary Emerg. Dis. 59, 85-102.
An, D. J., Roh, I. S., Song, D. S., Park, C. K., Park, B. K., 2007. Phylogenetic characterization of porcine circovirus type 2 in PMWS and PDNS Korean pigs between 1999 and 2006. Virus Res.129, 115-122.
Gagnon, C. A., del Castillo, J. R., Music, N., Fontaine, G., Harel, J., Tremblay, D., 2008. Development and use of a multiplex real-time quantitative polymerase chain reaction assay for detection and differentiation of porcine circovirus-2 genotypes 2a and 2b in an epidemiological survey. J. Vet. Diagn Invest. 20, 545-558.
Lyoo, K. S., Kim, H. B., Joo, H. S., 2008. Evaluation of a nested polymerase chain reaction assay to differentiate between two genotypes of porcine circovirus-2. J. Vet. Diagn Invest. 20, 283-288.
Wang, C., Pang, V. F., Lee, F., Liao, P. C., Huang, Y. L., Lai, S. S., Jeng, C. R., 2014. Development and evaluation of a loop-mediated isothermal amplification method for rapid detection and differentiation of two genotypes of porcine circovirus type 2. J. Microbiol. Immunol. Infect. 47, 363-370.
Tao, C., Yang, Y., Li, X., Zheng, X., Ren, H., Li, K., Zhou, R., 2016. Rapid and sensitive detection of sFAT-1 transgenic pigs by visual loop-mediated isothermal amplification. Appl. Biochem. Biotechnol. 179, 938-946.
Woźniakowski, G., Tarasiuk, K., 2015. Visual detection of goose haemorrhagic polyomavirus in geese and ducks by loop-mediatedisothermal amplification. Avian Pathol. 44, 311-318.
Liu, X., Zhou, Y., Yang, F., Liu, P., Cai, Y., Huang, J., Zhu, L., Xu, Z., 2016. Rapid and sensitive detection of porcine torovirus by a reverse transcription loop mediated isothermal amplification assay (RT-LAMP). J. Virol. Methods 228, 103-107.
Wang, C., Lee, F., Huang, T. S., Pan, C. H., Jong, M. H., Chao, P. H., 2008. Genetic variation in open reading frame 5 gene of porcine reproductive and respiratory syndrome virus in Taiwan. Vet. Microbiol. 131, 339-347.
Xiao, C. T., Halbur, P. G., Opriessnig, T., 2012. Complete genome sequence of a novel porcine circovirus type 2b variant present in cases of vaccine failures in the United States. J. Virol. 86, 12469.
Harding, J. C., Ellis, J. A., McIntosh, K. A., Krakowka, S., 2010. Dual heterologous porcine circovirus genogroup 2a/2b infection induces severe disease in germ-free pigs. Vet. Microbiol. 145, 209-219.
Lefebvre, D. J, Costers, S., Van Doorsselaere, J., Misinzo, G., Delputte, P. L., Nauwynck, H. J., 2008. Antigenic differences among porcine circovirus type 2 strains, as demonstrated by the use of monoclonal antibodies. J. Gen. Virol. 89, 177-187.
Opriessnig, T., Xiao, C. T., Gerber, P. F., Halbur, P. G., 2013. Emergence of a novel mutant PCV2b variant associated with clinical PCVAD in two vaccinated pig farms in the U. S. concurrently infected with PPV2. Vet. Microbiol. 163, 177-183.