Animal and Veterinary Sciences
Volume 6, Issue 2, March 2018, Pages: 27-34
Received: Apr. 20, 2018;
Accepted: May 17, 2018;
Published: Jun. 15, 2018
Views 1377 Downloads 66
Enefe Ndidi Glory, Department of Veterinary Physiology and Biochemistry, Faculty of Veterinary Medicine, University of Abuja, Abuja, Nigeria
Ebuehi Osaretin Albert, Department of Biochemistry, College of Medicine, University of Lagos, Idiaraba, Lagos State Nigeria
The study was carried out to evaluate the effect of alloxan–induced diabetes on some oxidative stress markers in rabbits. Ten rabbits weighing between 1.0-1.5kg were used and divided into two groups of five rabbits each. Diabetes was induced in one of the groups, by intraperitonial administration of alloxan at a dosage of 150mg/kg body weight and the control rabbits were administered normal saline (0.9% NaCl). The animals were monitored for 14days after confirmed diabetes in the test group. The fasting plasma glucose, plasma insulin, plasma lipids, the antioxidant enzymes: Superoxide dismutase (SOD), catalase, and lipid peroxidation were assayed for, in the plasma and the brain of the diabetic and non-diabetic rabbits. The results showed a significant decrease in mean body weight of the alloxan-induced diabetic and control rabbits pre and 14days post alloxan-induction. There was a significant (p < 0.01) increase in fasting plasma glucose, a significant (p < 0.01) decrease in the activity of SOD and a significant (p < 0.01) increase in the activity of catalase in the plasma and brain; there was also a significant (p < 0.01) increase in the level of lipid peroxidation, plasma cholesterol and triglycerides in the diabetic rabbits. Culminating a depletion of SOD activity, the altered activity of plasma (or brain) catalase and increased lipid peroxidation is due to the oxidative stress induced by the diabetic condition, hence the present study indicate that alloxan – induced diabetic potentiated oxidative stress in a short term duration. This shows the importance of antioxidant mimetics to be administered at early stage of diabetics in other to increase the antioxidant capacity thus preventing oxidative stress which may lead to complication in diabetes.
Enefe Ndidi Glory,
Ebuehi Osaretin Albert,
Antioxidant Enzymes and Lipid Peroxidation in Alloxan-Induced Diabetic Rabbits, Animal and Veterinary Sciences.
Vol. 6, No. 2,
2018, pp. 27-34.
Bennett, P. H (1994) Definition Diagnosis and classification of Diabetes and impaired glucose tolerance. In joslins diabetes mellitus, ed. by C. kahn and G Weir, 13th ed. Philadelphia, USA, Lea and Ferbiger, 1: p193.
King, H. R., Aubert, E and Herman, W. H. (1998) Global Burden of diabetics (1995-2005) Prevalence, Numerical Estimation and Projection Diabetics care; Vol. 21: 1411-11431.
World Health Organization (2003) Screening for Diabetes: Report of a World Health Organization and international Diabetes federation Meeting. WHO/NHM/MNC/03.1.http://www.who.int/diabetes/publications/en/screening_mnc03.pdf.
Bredleharm H. W. (2010) Effect of an Anti-diabetic extract of catharanthos on enzyme activities in Alloxan induced diabetic rats. Journal of Ethno pharmacological Center for Diabetes Control and Prevention 4th Ed.:45-57.
Giacco, F and Brownlee, M. (2010) Oxidative stress and Diabetic Complications. Circulation Research 107 (9): 1058-1070.
Bhattacharya, S (2015). Reactive Oxygen Species and Cellular Defense Sysetem. In V. Rani and U. C. S. Yadav (eds.), Free Radicals in Human Health and Disease, pp 17-29.
Rahbani, N. (1999). Total Antioxidant capacity and Glutathione peroxidase in Diabetic patients. Medicinal Journal of Islamic Academy of Science 12 (4): 109-114.
Zimmermann, M. (1983). Ethical guidelines for investigations of Experimental pain in Conscious Animals. Pain, 16: 109-110.
Aksnes, A and Njaa, L. R. (1981). Catalase, Glutathione peroxidase and Superoxide dismutase in different fish species. Comparative Biochemistry and Physiology, 69B: 893–896.
Cooper, G. R., (1973). Methods for Determining the amount of Glucose in blood. Critical Review of Clinical Laboratory Science, 4: 101-145.
Yallow, R. S and Bearson S. A, (1960) Immunoassay of endogenous plasma in man. Journal of Clinical Investigation. 39: 1157-1175.
Misra, H. P. and Fridovich, (1972) The role of superoxide ion in the autoxidation of epinephrine and a simple assay for superoxide dismutase. Journal of Biological Chemistry 247: 3174-3175.
Aebi, H., (1967). Catalase: In Methods of enzymatic analysis. edited by Bermeyer H. U. p673-684.
Stein, E. A. (1986): In textbook of clinical chemistry, NW Tiez, Ed. W. B. Sander, Philadelphia, p. p. 879-886.
Stravon Poulous, W. S and Crouch R. D (1974) A New Colourimeter procedure for the determination of Serum triglyceride. Clinical chemistry 20: 857.
Niehaus, W. G., and Samuelsson, B. (1968). Formation of malonaldehyde from phospholipid arachidonate during microsomal lipid peroxidation. European Journal of Biochemistry. 6:126-130.
Rerrup, C. C. (1990) Drugs producing diabetes through damage of insulin secreting cells. Pharmacological Research; 22: 485-520.
Shinn, S. H. (1998) Oxidative stress and diabetes complications In; Recent advances and pathogenesis and management of diabetes mellitus. 1st ed science co, Singapore. P 3-8.
He L., He T., Farrar, S., Ji, L., Liu, T. and Ma X. (2017). Antioxidants Maintain Cellular Redox Homeostasis by Elimination of Reactive Oxygen Species. Cellular Physiology and Biochemistry 14; 532-55.
West, I. C (2000). Radicals and Oxidative stress in Diabetes. Diabetic Medicine17: 171-180.
Uttara, B Singh A, V., Zamboni, P and Mahajan R. T (2009) (Oxidative Stress and NeurodegenerativeDiseases: A Review of Upstream and Downstream Antioxidant Therapeutic Options. Current Neuropharmacology 7 (1): 65–74.
Elmegeed, G. A., Ahmed, H. A and Hussein, J. S. (2005). Novel synthesized aminosteroidal heterocycles intervention for inhibiting iron-induced oxidative stress. European Journal of Medicinal Chemistry 40 (12): 1283-1294.
Granner, D. K (1996) Hormones of Pancreas and Gastrointestinal tract Jn: Murrray R. K, ed 24. Connecticut USA Appletion and lange. 24: 586-587.
Yu, B. J and Cho, H. C (1993) The activities of erythrocyte antioxidant enzymes in Diabetes mellitus. Korean Journal of internal Medicine Vol 44 p766-774.
Ngaski, A. A (2018). Correlation of Antioxidants Enzymes Activity with Fasting Blood Glucose in Diabetic Patients in Sokoto, Nigeria. British Journal of Medicine and Medical Research, 25 (12):1-6.
Dominigues, C., Ruiz E., Gussinye, M and Carroscossa, A (1990). Oxidative stress at the onset in early stages of type 1 Diabetes in children. Diabetes Care: Vol 21 p1736-1742.
King G. L., Kunisaki. M., Nishio. Y. Inoguch. T., Shiba T. (1996) Biochemical and Molecular mechanisms in the development of diabetes vascular complications. Diabetes. 45: 105-108.
Matough, F. A Budin, S. B., Hamid Z. A, Alwahaibi, N and Mohamed J. (2012). The Role of Oxidative Stress and Antioxidants in Diabetic Complications. Sultan Qaboos University Medical Journal. 12 (1): 5–18.
Genet, S., Kale, R. K., Bacquer, N. Z (2002) Alterations in Antioxidant Enzymes and Oxidative damage in experimental diabetic rat tissues; Effect of Vanadate and fenugreek. Molecular and Cellular Biochemistry 236: 7-12.
Pari, L and Umamaheswari, J (2000). Anti Hyperglyceamic activity of Musa sapientum flowers: effect on lipid peroxidation in alloxan diabetic rats. Phytotherapy Research 14 (2):136-138.
Lehninger, A. L., Nelson D. L and M. M, (1993) Principles of Biochemistry 2nd ed. Worth Publishers p760.
Vessby, T. Basu, S., Mohsen, R., Berne C., Vessby, B. (2002). Oxidative stress and antioxidant status in type 1 diabetes mellitus. Journal of Internal Medicine 251: 69-76.
Nna., V. U, Oka, V., O., Aluko E. O. and Helen., O. T, (2013) Comparative Effects of Aqueous Leaf of Viscum Album (mistletoe) and Aleo vera gel in the management of Streptozocin-induced diabetes mellitus. International Journal of Diabetes Research, 3 (4):2306-2316.
Onunogbo, C., Ohaeri., O. C, Eleazu, C. O., Eleazu K C. (2012) Chemical composition of mistletoe extract (Loranthus micranthus) and its effect on the protein, lipid metabolism and the antioxidant status of alloxan induced diabetic rats. E3 Journal of Medical Research; 1 (4):057-062.