Complicated Effect of He-Ne Laser Therapy on Pro-/Anti-Inflammatory Cytokines from Serum in Rats

Wuren Ma; Yunpeng Fan; Jinyang Zuo; Zhao Chen; Jun Yuan; Xiaoping Song

doi:doi:10.11648/j.avs.20180605.14

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Complicated Effect of He-Ne Laser Therapy on Pro-/Anti-Inflammatory Cytokines from Serum in Rats

Wuren Ma, Yunpeng Fan, Jinyang Zuo, Zhao Chen, Jun Yuan, Xiaoping Song

Published in Animal and Veterinary Sciences (Volume 6, Issue 5)

Received: 23 October 2018 Accepted: 9 November 2018 Published: 5 December 2018

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Abstract

Helium-neon (He-Ne) laser is one of the most common low energy lasers used in physiotherapy for promoting wound healing. This study was about to investigate the effects of He-Ne laser therapy on the pro-/anti-inflammatory cytokines in rats up to 10 days of treatment. 16 non-pregnant female rats were divided into 4 groups randomly, 4 rats in each group. They are negative control (NC) group, positive control (PC) group, laser treatment (LA) group and erythromycin ointment (ER) treatment group. The LA group was treated with 25 mW of He-Ne laser for 30 min every day for 10 days, with 632.8 nm of wavelength immediately after three incisions were made on the mid-dorsal line of each rat. The results showed that He-Ne laser therapy could decrease the level of IL-1β compared with the PC and ER groups; while increase the level of IL-6 compared with the NC and ER groups, and the level of TNF-α compared with the PC and ER group. He-Ne laser therapy also increased the concentration of IL-10 compared with the other three groups, and the concentration of TGF-β compared with the NC and ER groups. These results indicated that laser acupuncture increased the production of IL-6, IL-10, TGF-β and TNF-α after 10 days of treatment, which was the proliferation stage of wound healing process, and the effect of He-Ne laser therapy on pro-inflammatory cytokines was not always consistent, which might be time-dependent of He-Ne laser treatment.

Published in	Animal and Veterinary Sciences (Volume 6, Issue 5)
DOI	10.11648/j.avs.20180605.14
Page(s)	88-94
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

He-Ne Laser Therapy, Pro-inflammatory Cytokines, Anti-inflammatory Cytokines, Wound Healing

References

[1]	Loreti E H, Pascoal V L W, Nogueira B V, et al., 2015. Use of Laser Therapy in the Healing Process: A Literature Review. Photomedicine Laser Surgery. 33, 104-116.
[2]	Thawer H A, Houghton P E, 1999. Effect of laser irradiation on the growth and development of fetal mouse limbs in an in vitro model. Lasers in Surgery and Medicine. 24, 285-295.
[3]	Hubacek J, Dusek J, Grivnova J, et al., 1985. The effect of the He-Ne laser on wound healing. Ceska Otolaryngology. 34, 135-140.
[4]	Ricevuti G, Mazzone A, Degiulio R, et al., 1988. Effects of He-Ne laser on the inflammatory response in vivo and in vitro. Medicina. 8, 167-168.
[5]	Maiya G A, Kumar P, Rao L., 2005. Effect of low intensity helium-neon (He-Ne) laser irradiation on diabetic wound healing dynamics. Photomedicine Laser Surgery. 23, 187-190.
[6]	Gal P, Kilik R, Spakova T, et al., 2005. He-Ne laser irradiation accelerates inflammatory phase and epithelization of skin wound healing in rats. Biologia. 60, 691-696.
[7]	Goncalves W L, Souza F M, Conti C L, et al., 2007. Influence of He-Ne laser therapy on the dynamics of wound healing in mice treated with anti-inflammatory drugs. Brazilian Journal of Medical and Biological Research. 40, 877-884.
[8]	Campana V, Moya M, Gavotto A, et al., 1998. Effects of diclofenac sodium and He: Ne laser irradiation on plasmatic fibrinogen levels in inflammatory processes. Journal of Clinical Laser Medicine and Surgery. 16, 317-320.
[9]	Nedrebo T, Reed RK, Jonsson R, et al., 2004. Differential cytokine response in interstitial fluid in skin and serum during experimental inflammation in rats. Journal of Physiology. 556, 193-202.
[10]	Jin Y, Wi H J, Choi M H, et al., 2014. Regulation of anti-inflammatory cytokines IL-10 and TGF-beta in mouse dendritic cells through treatment with Clonorchis sinensis crude antigen. Experimental and Molecular Medicine. 46, e74.
[11]	Safavi S M, Kazemi B, Esmaeili M, et al., 2008. Effects of low-level He-Ne laser irradiation on the gene expression of IL-1 beta, TNF-alpha, IFN-gamma, TGF-beta, bFGF, and PDGF in rat's gingiva. Lasers in Medical Science. 23, 331-335.
[12]	Lima A A M, Spinola L G, Baccan G, et al., 2014. Evaluation of corticosterone and IL-1 beta, IL-6, IL-10 and TNF-alpha expression after 670-nm laser photo biomodulation in rats. Lasers in Medical Science. 29, 709-715.
[13]	Pires D, Xavier M, Araujo T, et al., 2011. Low-level laser therapy (LLLT, 780 nm) acts differently on mRNA expression of anti- and pro-inflammatory mediators in an experimental model of collagenase-induced tendinitis in rat. Lasers in Medical Science. 26, 85-94.
[14]	Novoselova E G, Glushkova O V, Cherenkov D A, et al., 2006. Effects of low-power laser radiation on mice immunity. Photodermatology Photoimmunology Photomedicine. 22, 33-38.
[15]	Song G D, Nguyen D T, Pietramaggiori G, et al., 2010. Use of the parabiotic model in studies of cutaneous wound healing to define the participation of circulating cells. Wound Repair and Regeneration. 18, 426-432.
[16]	Reinke J M, Sorg H, 2012. Wound repair and regeneration. European Surgical Research. 49, 35-43.
[17]	Lin Z Q, Kondo T, Ishida Y, et al., 2003. Essential involvement of IL-6 in the skin wound-healing process as evidenced by delayed wound healing in IL-6-deficient mice. Journal of Leukocyte Biology. 73, 713-721.
[18]	Gallucci R M, Simeonova P P, Matheson J M, et al., 2000. Impaired cutaneous wound healing in interleukin-6-deficient and immunosuppressed mice. Faseb Journal. 14, 2525-2531.
[19]	Er H, Doganay S, Evereklioglu C, et al., 2002. Effects of L-NAME and timolol on aqueous IL-1 beta, IL-6 IL-8, TNF-alpha and NO levels after Nd: YAG laser iridotomy in rabbits. European Journal of Ophthalmology. 12, 281-286.
[20]	Dominguez A, Gomez C, Garcia-Kass A I, et al., 2010. IL-1 beta, TNF-alpha, Total Antioxidative Status and Microbiological Findings in Chronic Periodontitis Treated With Fluorescence-Controlled Er: YAG Laser Radiation. Lasers in Surgery and Medicine. 42, 24-31.
[21]	Mesquita-Ferrari R A, Martins M D, Silva J A J, et al., 2011. Effects of low-level laser therapy on expression of TNF-alpha and TGF-beta in skeletal muscle during the repair process. Lasers in Medical Science. 26, 335-340.
[22]	Xu F, Zhang C, Graves D T, 2013. Abnormal cell responses and role of TNF-alpha in impaired diabetic wound healing. Biomed Research International. 2013, 754802.
[23]	Wang H J, Ding Y C, 2003. A analysis of ELISA on the time-related expression of IL-2 and TNF-alpha during the healing process of wound in rat skin. Journal of Forensic Medicine. 19, 10-12.
[24]	Opal S M, DePalo V A, 2000. Anti-inflammatory cytokines. Chest. 117, 1162-1172.
[25]	Hameedaldeen A, Liu J, Batres A, et al., 2014. FOXO1, TGF-beta regulation and wound healing. International Journal of Molecluar Sciences. 15, 16257-12669.
[26]	Suga H, Sugaya M, Fujita H, et al., 2014. TLR4, rather than TLR2, regulates wound healing through TGF-beta and CCL5 expression. Journal of Dermatological Science. 73, 117-124.
[27]	Zhang H, Zhu S H, Qin Q S, 2004. Immunohistochemical and morphometrical study on the expression of interleukin-10 (IL-10) in different expressive parts during cutaneous wound healing in mice. Journal of Forensic Medicine. 20, 70-72.
[28]	Oliveira R G, Ferreira A P, Cortes A J, et al., 2013. Low-level laser reduces the production of TNF-alpha, IFN-gamma, and IL-10 induced by OVA. Laser in Medical Science. 28, 1519-1525.
[29]	Hentschke V S, Jaenisch R B, Schmeing L A, et al., 2011. Low level laser therapy decreased TNF-alpha and IL-6 and increased IL-10 levels and IL-10/TNF-alpha ratio in the skeletal muscle of rats with heart failure. European Heart Journal. 32, 976-977.

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APA Style

Wuren Ma, Yunpeng Fan, Jinyang Zuo, Zhao Chen, Jun Yuan, et al. (2018). Complicated Effect of He-Ne Laser Therapy on Pro-/Anti-Inflammatory Cytokines from Serum in Rats. Animal and Veterinary Sciences, 6(5), 88-94. https://doi.org/10.11648/j.avs.20180605.14

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ACS Style

Wuren Ma; Yunpeng Fan; Jinyang Zuo; Zhao Chen; Jun Yuan, et al. Complicated Effect of He-Ne Laser Therapy on Pro-/Anti-Inflammatory Cytokines from Serum in Rats. Anim. Vet. Sci. 2018, 6(5), 88-94. doi: 10.11648/j.avs.20180605.14

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AMA Style

Wuren Ma, Yunpeng Fan, Jinyang Zuo, Zhao Chen, Jun Yuan, et al. Complicated Effect of He-Ne Laser Therapy on Pro-/Anti-Inflammatory Cytokines from Serum in Rats. Anim Vet Sci. 2018;6(5):88-94. doi: 10.11648/j.avs.20180605.14

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@article{10.11648/j.avs.20180605.14,
  author = {Wuren Ma and Yunpeng Fan and Jinyang Zuo and Zhao Chen and Jun Yuan and Xiaoping Song},
  title = {Complicated Effect of He-Ne Laser Therapy on Pro-/Anti-Inflammatory Cytokines from Serum in Rats},
  journal = {Animal and Veterinary Sciences},
  volume = {6},
  number = {5},
  pages = {88-94},
  doi = {10.11648/j.avs.20180605.14},
  url = {https://doi.org/10.11648/j.avs.20180605.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.avs.20180605.14},
  abstract = {Helium-neon (He-Ne) laser is one of the most common low energy lasers used in physiotherapy for promoting wound healing. This study was about to investigate the effects of He-Ne laser therapy on the pro-/anti-inflammatory cytokines in rats up to 10 days of treatment. 16 non-pregnant female rats were divided into 4 groups randomly, 4 rats in each group. They are negative control (NC) group, positive control (PC) group, laser treatment (LA) group and erythromycin ointment (ER) treatment group. The LA group was treated with 25 mW of He-Ne laser for 30 min every day for 10 days, with 632.8 nm of wavelength immediately after three incisions were made on the mid-dorsal line of each rat. The results showed that He-Ne laser therapy could decrease the level of IL-1β compared with the PC and ER groups; while increase the level of IL-6 compared with the NC and ER groups, and the level of TNF-α compared with the PC and ER group. He-Ne laser therapy also increased the concentration of IL-10 compared with the other three groups, and the concentration of TGF-β compared with the NC and ER groups. These results indicated that laser acupuncture increased the production of IL-6, IL-10, TGF-β and TNF-α after 10 days of treatment, which was the proliferation stage of wound healing process, and the effect of He-Ne laser therapy on pro-inflammatory cytokines was not always consistent, which might be time-dependent of He-Ne laser treatment.},
 year = {2018}
}

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TY  - JOUR
T1  - Complicated Effect of He-Ne Laser Therapy on Pro-/Anti-Inflammatory Cytokines from Serum in Rats
AU  - Wuren Ma
AU  - Yunpeng Fan
AU  - Jinyang Zuo
AU  - Zhao Chen
AU  - Jun Yuan
AU  - Xiaoping Song
Y1  - 2018/12/05
PY  - 2018
N1  - https://doi.org/10.11648/j.avs.20180605.14
DO  - 10.11648/j.avs.20180605.14
T2  - Animal and Veterinary Sciences
JF  - Animal and Veterinary Sciences
JO  - Animal and Veterinary Sciences
SP  - 88
EP  - 94
PB  - Science Publishing Group
SN  - 2328-5850
UR  - https://doi.org/10.11648/j.avs.20180605.14
AB  - Helium-neon (He-Ne) laser is one of the most common low energy lasers used in physiotherapy for promoting wound healing. This study was about to investigate the effects of He-Ne laser therapy on the pro-/anti-inflammatory cytokines in rats up to 10 days of treatment. 16 non-pregnant female rats were divided into 4 groups randomly, 4 rats in each group. They are negative control (NC) group, positive control (PC) group, laser treatment (LA) group and erythromycin ointment (ER) treatment group. The LA group was treated with 25 mW of He-Ne laser for 30 min every day for 10 days, with 632.8 nm of wavelength immediately after three incisions were made on the mid-dorsal line of each rat. The results showed that He-Ne laser therapy could decrease the level of IL-1β compared with the PC and ER groups; while increase the level of IL-6 compared with the NC and ER groups, and the level of TNF-α compared with the PC and ER group. He-Ne laser therapy also increased the concentration of IL-10 compared with the other three groups, and the concentration of TGF-β compared with the NC and ER groups. These results indicated that laser acupuncture increased the production of IL-6, IL-10, TGF-β and TNF-α after 10 days of treatment, which was the proliferation stage of wound healing process, and the effect of He-Ne laser therapy on pro-inflammatory cytokines was not always consistent, which might be time-dependent of He-Ne laser treatment.
VL  - 6
IS  - 5
ER  -

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Author Information

Wuren Ma

College of Veterinary Medicine, Northwest A&F University, Yangling, P R China; Xi’an Veterinary Teaching Hospital, Northwest A&F University, Xi’an, P R China
Yunpeng Fan

College of Veterinary Medicine, Northwest A&F University, Yangling, P R China
Jinyang Zuo

College of Veterinary Medicine, Northwest A&F University, Yangling, P R China
Zhao Chen

College of Veterinary Medicine, Northwest A&F University, Yangling, P R China
Jun Yuan

Institute of Fisheries Engineering, Chinese Academy of Fishery Sciences, Beijing, P R China
Xiaoping Song

College of Veterinary Medicine, Northwest A&F University, Yangling, P R China

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APA Style

Wuren Ma, Yunpeng Fan, Jinyang Zuo, Zhao Chen, Jun Yuan, et al. (2018). Complicated Effect of He-Ne Laser Therapy on Pro-/Anti-Inflammatory Cytokines from Serum in Rats. Animal and Veterinary Sciences, 6(5), 88-94. https://doi.org/10.11648/j.avs.20180605.14

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ACS Style

Wuren Ma; Yunpeng Fan; Jinyang Zuo; Zhao Chen; Jun Yuan, et al. Complicated Effect of He-Ne Laser Therapy on Pro-/Anti-Inflammatory Cytokines from Serum in Rats. Anim. Vet. Sci. 2018, 6(5), 88-94. doi: 10.11648/j.avs.20180605.14

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AMA Style

Wuren Ma, Yunpeng Fan, Jinyang Zuo, Zhao Chen, Jun Yuan, et al. Complicated Effect of He-Ne Laser Therapy on Pro-/Anti-Inflammatory Cytokines from Serum in Rats. Anim Vet Sci. 2018;6(5):88-94. doi: 10.11648/j.avs.20180605.14

Copy | Download

@article{10.11648/j.avs.20180605.14,
  author = {Wuren Ma and Yunpeng Fan and Jinyang Zuo and Zhao Chen and Jun Yuan and Xiaoping Song},
  title = {Complicated Effect of He-Ne Laser Therapy on Pro-/Anti-Inflammatory Cytokines from Serum in Rats},
  journal = {Animal and Veterinary Sciences},
  volume = {6},
  number = {5},
  pages = {88-94},
  doi = {10.11648/j.avs.20180605.14},
  url = {https://doi.org/10.11648/j.avs.20180605.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.avs.20180605.14},
  abstract = {Helium-neon (He-Ne) laser is one of the most common low energy lasers used in physiotherapy for promoting wound healing. This study was about to investigate the effects of He-Ne laser therapy on the pro-/anti-inflammatory cytokines in rats up to 10 days of treatment. 16 non-pregnant female rats were divided into 4 groups randomly, 4 rats in each group. They are negative control (NC) group, positive control (PC) group, laser treatment (LA) group and erythromycin ointment (ER) treatment group. The LA group was treated with 25 mW of He-Ne laser for 30 min every day for 10 days, with 632.8 nm of wavelength immediately after three incisions were made on the mid-dorsal line of each rat. The results showed that He-Ne laser therapy could decrease the level of IL-1β compared with the PC and ER groups; while increase the level of IL-6 compared with the NC and ER groups, and the level of TNF-α compared with the PC and ER group. He-Ne laser therapy also increased the concentration of IL-10 compared with the other three groups, and the concentration of TGF-β compared with the NC and ER groups. These results indicated that laser acupuncture increased the production of IL-6, IL-10, TGF-β and TNF-α after 10 days of treatment, which was the proliferation stage of wound healing process, and the effect of He-Ne laser therapy on pro-inflammatory cytokines was not always consistent, which might be time-dependent of He-Ne laser treatment.},
 year = {2018}
}

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TY  - JOUR
T1  - Complicated Effect of He-Ne Laser Therapy on Pro-/Anti-Inflammatory Cytokines from Serum in Rats
AU  - Wuren Ma
AU  - Yunpeng Fan
AU  - Jinyang Zuo
AU  - Zhao Chen
AU  - Jun Yuan
AU  - Xiaoping Song
Y1  - 2018/12/05
PY  - 2018
N1  - https://doi.org/10.11648/j.avs.20180605.14
DO  - 10.11648/j.avs.20180605.14
T2  - Animal and Veterinary Sciences
JF  - Animal and Veterinary Sciences
JO  - Animal and Veterinary Sciences
SP  - 88
EP  - 94
PB  - Science Publishing Group
SN  - 2328-5850
UR  - https://doi.org/10.11648/j.avs.20180605.14
AB  - Helium-neon (He-Ne) laser is one of the most common low energy lasers used in physiotherapy for promoting wound healing. This study was about to investigate the effects of He-Ne laser therapy on the pro-/anti-inflammatory cytokines in rats up to 10 days of treatment. 16 non-pregnant female rats were divided into 4 groups randomly, 4 rats in each group. They are negative control (NC) group, positive control (PC) group, laser treatment (LA) group and erythromycin ointment (ER) treatment group. The LA group was treated with 25 mW of He-Ne laser for 30 min every day for 10 days, with 632.8 nm of wavelength immediately after three incisions were made on the mid-dorsal line of each rat. The results showed that He-Ne laser therapy could decrease the level of IL-1β compared with the PC and ER groups; while increase the level of IL-6 compared with the NC and ER groups, and the level of TNF-α compared with the PC and ER group. He-Ne laser therapy also increased the concentration of IL-10 compared with the other three groups, and the concentration of TGF-β compared with the NC and ER groups. These results indicated that laser acupuncture increased the production of IL-6, IL-10, TGF-β and TNF-α after 10 days of treatment, which was the proliferation stage of wound healing process, and the effect of He-Ne laser therapy on pro-inflammatory cytokines was not always consistent, which might be time-dependent of He-Ne laser treatment.
VL  - 6
IS  - 5
ER  -

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