Animal and Veterinary Sciences

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Effects of a Single Dose of Ethanol on Survival Rate and Angiogenesis of Chick Embryo

Received: 05 September 2014    Accepted: 03 December 2014    Published: 20 January 2015
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Abstract

The developing Chick has been widely used as a model organism for research studies in developmental biology. Various aspects of ethanol (EtOH) toxicity during embryonic development have been documented in experimental studies. The studies on chick embryo address the effects of EtOH on craniofacial abnormalities and angiogenesis. The purpose of this study was to assess the effects of a single dose of ethanol on survival rate and angiogenesis of chick embryos during early stages of development. Chick eggs were incubated at 37ºC in the humidified incubator, after 72 hours of incubation these eggs were removed from incubator, and treated with a single doses of 1%, 5%, 10%, 13% and 15% EtOH. The effects on survival rate and angiogenesis were recorded on ethanol treated chick embryos. The survival rate was slightly reduced with 1% and 5% EtOH, however with increasing dose of 10% the survival rate was reduced to 64% and at 15% EtOH complete death was observed. Our results also indicate that treatment of EtOH with 1% and 5% did not have any obvious effects on vessels formation in comparison with the BSS treated embryos. However, treatment of chick embryo with 10%, 13% and 15% EtOH severely inhibited the blood vessels formation.

DOI 10.11648/j.avs.20150301.12
Published in Animal and Veterinary Sciences (Volume 3, Issue 1, January 2015)
Page(s) 8-11
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Chick Embryo, Angiogenesis, EtOH

References
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Author Information
  • Department of Physiology, University of Sindh, Jamshoro, Sindh, Pakistan

  • Department of Physiology, University of Sindh, Jamshoro, Sindh, Pakistan

  • Department of Fresh Water Biology and fisheries, University of Sindh, Jamshoro, Sindh, Pakistan

  • Department of Fresh Water Biology and fisheries, University of Sindh, Jamshoro, Sindh, Pakistan

  • Department of Fresh Water Biology and fisheries, University of Sindh, Jamshoro, Sindh, Pakistan

  • Centre for Environmental Sciences, University of Sindh, Jamshoro, Sindh, Pakistan

  • Department of Fresh Water Biology and fisheries, University of Sindh, Jamshoro, Sindh, Pakistan

  • Department of Zoology, University of Sindh, Jamshoro, Sindh, Pakistan

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    Zulifqar Ali Laghari, Ayaz Ali Samo, Baradi Waryani, Zameer Ali Palh, Khalid Hussain Lashari, et al. (2015). Effects of a Single Dose of Ethanol on Survival Rate and Angiogenesis of Chick Embryo. Animal and Veterinary Sciences, 3(1), 8-11. https://doi.org/10.11648/j.avs.20150301.12

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    ACS Style

    Zulifqar Ali Laghari; Ayaz Ali Samo; Baradi Waryani; Zameer Ali Palh; Khalid Hussain Lashari, et al. Effects of a Single Dose of Ethanol on Survival Rate and Angiogenesis of Chick Embryo. Anim. Vet. Sci. 2015, 3(1), 8-11. doi: 10.11648/j.avs.20150301.12

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    AMA Style

    Zulifqar Ali Laghari, Ayaz Ali Samo, Baradi Waryani, Zameer Ali Palh, Khalid Hussain Lashari, et al. Effects of a Single Dose of Ethanol on Survival Rate and Angiogenesis of Chick Embryo. Anim Vet Sci. 2015;3(1):8-11. doi: 10.11648/j.avs.20150301.12

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  • @article{10.11648/j.avs.20150301.12,
      author = {Zulifqar Ali Laghari and Ayaz Ali Samo and Baradi Waryani and Zameer Ali Palh and Khalid Hussain Lashari and Ghulam Murtaza Mastoi and Gulshan Ara Sahato and Tahira Jabeen Ursani},
      title = {Effects of a Single Dose of Ethanol on Survival Rate and Angiogenesis of Chick Embryo},
      journal = {Animal and Veterinary Sciences},
      volume = {3},
      number = {1},
      pages = {8-11},
      doi = {10.11648/j.avs.20150301.12},
      url = {https://doi.org/10.11648/j.avs.20150301.12},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.avs.20150301.12},
      abstract = {The developing Chick has been widely used as a model organism for research studies in developmental biology. Various aspects of ethanol (EtOH) toxicity during embryonic development have been documented in experimental studies. The studies on chick embryo address the effects of EtOH on craniofacial abnormalities and angiogenesis. The purpose of this study was to assess the effects of a single dose of ethanol on survival rate and angiogenesis of chick embryos during early stages of development. Chick eggs were incubated at 37ºC in the humidified incubator, after 72 hours of incubation  these eggs were removed from incubator, and treated with a single doses of 1%, 5%, 10%, 13% and 15% EtOH. The effects on survival rate and angiogenesis were recorded on ethanol treated chick embryos. The survival rate was slightly reduced with 1% and 5% EtOH, however with increasing dose of 10% the survival rate was reduced to 64% and at 15% EtOH complete death was observed. Our results also indicate that treatment of EtOH with 1% and 5% did not have any obvious effects on vessels formation in comparison with the BSS treated embryos. However, treatment of chick embryo with 10%, 13% and 15% EtOH severely inhibited the blood vessels formation.},
     year = {2015}
    }
    

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  • TY  - JOUR
    T1  - Effects of a Single Dose of Ethanol on Survival Rate and Angiogenesis of Chick Embryo
    AU  - Zulifqar Ali Laghari
    AU  - Ayaz Ali Samo
    AU  - Baradi Waryani
    AU  - Zameer Ali Palh
    AU  - Khalid Hussain Lashari
    AU  - Ghulam Murtaza Mastoi
    AU  - Gulshan Ara Sahato
    AU  - Tahira Jabeen Ursani
    Y1  - 2015/01/20
    PY  - 2015
    N1  - https://doi.org/10.11648/j.avs.20150301.12
    DO  - 10.11648/j.avs.20150301.12
    T2  - Animal and Veterinary Sciences
    JF  - Animal and Veterinary Sciences
    JO  - Animal and Veterinary Sciences
    SP  - 8
    EP  - 11
    PB  - Science Publishing Group
    SN  - 2328-5850
    UR  - https://doi.org/10.11648/j.avs.20150301.12
    AB  - The developing Chick has been widely used as a model organism for research studies in developmental biology. Various aspects of ethanol (EtOH) toxicity during embryonic development have been documented in experimental studies. The studies on chick embryo address the effects of EtOH on craniofacial abnormalities and angiogenesis. The purpose of this study was to assess the effects of a single dose of ethanol on survival rate and angiogenesis of chick embryos during early stages of development. Chick eggs were incubated at 37ºC in the humidified incubator, after 72 hours of incubation  these eggs were removed from incubator, and treated with a single doses of 1%, 5%, 10%, 13% and 15% EtOH. The effects on survival rate and angiogenesis were recorded on ethanol treated chick embryos. The survival rate was slightly reduced with 1% and 5% EtOH, however with increasing dose of 10% the survival rate was reduced to 64% and at 15% EtOH complete death was observed. Our results also indicate that treatment of EtOH with 1% and 5% did not have any obvious effects on vessels formation in comparison with the BSS treated embryos. However, treatment of chick embryo with 10%, 13% and 15% EtOH severely inhibited the blood vessels formation.
    VL  - 3
    IS  - 1
    ER  - 

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