Choreospondias axillaries, locally called Lapsi is a dioecous, deciduous fruit bearing plant, with multiple daily uses. The protease was extracted with 0.1M phosphate buffer of pH 7 and then precipitated successively with TCA and ammonium sulfate. Thus obtained ammonium sulfate precipitated fraction of protease was characterized. The protease from root showed maximum activity at pH 10, temperature at 35°C. The optimum time of incubation was 15 minute. A bell shaped curve was obtained for the effect of enzyme concentration with optimum enzyme 50µg. The Km and Vmax value of root were 1.53nM and 52.91pmol/min.
| Published in | Journal of Plant Sciences (Volume 1, Issue 3) |
| DOI | 10.11648/j.jps.20130103.14 |
| Page(s) | 39-42 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
Protease, Lapsi, Purification, Characterization, Isolation
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APA Style
Shrawan Kumar Upadhyay, Rubin Thapa Magar, Chandan Jung Thapa. (2013). Characterization of Protease Isolated from Root of Choreospondias Axillaries (Lapsi). Journal of Plant Sciences, 1(3), 39-42. https://doi.org/10.11648/j.jps.20130103.14
ACS Style
Shrawan Kumar Upadhyay; Rubin Thapa Magar; Chandan Jung Thapa. Characterization of Protease Isolated from Root of Choreospondias Axillaries (Lapsi). J. Plant Sci. 2013, 1(3), 39-42. doi: 10.11648/j.jps.20130103.14
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Download@article{10.11648/j.jps.20130103.14,
author = {Shrawan Kumar Upadhyay and Rubin Thapa Magar and Chandan Jung Thapa},
title = {Characterization of Protease Isolated from Root of Choreospondias Axillaries (Lapsi)},
journal = {Journal of Plant Sciences},
volume = {1},
number = {3},
pages = {39-42},
doi = {10.11648/j.jps.20130103.14},
url = {https://doi.org/10.11648/j.jps.20130103.14},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jps.20130103.14},
abstract = {Choreospondias axillaries, locally called Lapsi is a dioecous, deciduous fruit bearing plant, with multiple daily uses. The protease was extracted with 0.1M phosphate buffer of pH 7 and then precipitated successively with TCA and ammonium sulfate. Thus obtained ammonium sulfate precipitated fraction of protease was characterized. The protease from root showed maximum activity at pH 10, temperature at 35°C. The optimum time of incubation was 15 minute. A bell shaped curve was obtained for the effect of enzyme concentration with optimum enzyme 50µg. The Km and Vmax value of root were 1.53nM and 52.91pmol/min.},
year = {2013}
}
TY - JOUR T1 - Characterization of Protease Isolated from Root of Choreospondias Axillaries (Lapsi) AU - Shrawan Kumar Upadhyay AU - Rubin Thapa Magar AU - Chandan Jung Thapa Y1 - 2013/11/10 PY - 2013 N1 - https://doi.org/10.11648/j.jps.20130103.14 DO - 10.11648/j.jps.20130103.14 T2 - Journal of Plant Sciences JF - Journal of Plant Sciences JO - Journal of Plant Sciences SP - 39 EP - 42 PB - Science Publishing Group SN - 2331-0731 UR - https://doi.org/10.11648/j.jps.20130103.14 AB - Choreospondias axillaries, locally called Lapsi is a dioecous, deciduous fruit bearing plant, with multiple daily uses. The protease was extracted with 0.1M phosphate buffer of pH 7 and then precipitated successively with TCA and ammonium sulfate. Thus obtained ammonium sulfate precipitated fraction of protease was characterized. The protease from root showed maximum activity at pH 10, temperature at 35°C. The optimum time of incubation was 15 minute. A bell shaped curve was obtained for the effect of enzyme concentration with optimum enzyme 50µg. The Km and Vmax value of root were 1.53nM and 52.91pmol/min. VL - 1 IS - 3 ER -
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