Oxygen Consumption and Lipoxygenase Activity in Isolated Tomato Fruit Chromoplasts
Journal of Plant Sciences
Volume 2, Issue 1, February 2014, Pages: 5-8
Received: Nov. 28, 2013; Published: Jan. 10, 2014
Views 2861      Downloads 141
Authors
Djédoux Maxime Angaman, UFR Agroforesterie, Université Jean Lorougnon Guédé, Daloa, Côte d'Ivoire; Departament de Bioquímica i Biologia molecular, Facultat de Biologia, Universitat de Barcelona, Spain
Marta Renato, Departament de Biologia Vegetal, Facultat de Biologia, Universitat de Barcelona, Spain
Joaquín Azcón-Bieto, Departament de Biologia Vegetal, Facultat de Biologia, Universitat de Barcelona, Spain
Albert Boronat, Departament de Bioquímica i Biologia molecular, Facultat de Biologia, Universitat de Barcelona, Spain; Centre de Recerca en Agrigenòmica (CRAG), Consorci CSIC-IRTA-UAB-UB, Campus Universitat Autònoma de Barcelona, Bellaterra - Cerdanyola del Vallès, 08193 – Barcelona, Spain
Article Tools
PDF
Follow on us
Abstract
This Study using purified tomato fruit chromoplasts has shown significant rates of oxygen consumption even in the absence of external precursors. Oxygen consumption rates increased up to 7-fold when chromoplast samples were incubated for 24 h at room temperature. This increase in oxygen consumption is most likely due to the activation of lipoxygenase in the chromoplasts.
Keywords
Chromoplasts, Lipoxygenase, Tomato, Oxygen Consumption
To cite this article
Djédoux Maxime Angaman, Marta Renato, Joaquín Azcón-Bieto, Albert Boronat, Oxygen Consumption and Lipoxygenase Activity in Isolated Tomato Fruit Chromoplasts, Journal of Plant Sciences. Vol. 2, No. 1, 2014, pp. 5-8. doi: 10.11648/j.jps.20140201.12
References
[1]
Thomson WW, Whatley JM (1980). Development of nongreen plastids. Ann Rev Plant Physiol., 31: 375-394.
[2]
Camara B, Hugueney P, Bouvier F, Kuntz M, Moneger R (1995). Biochemistry and molecular biology of chromoplast development. Int Rev Cytol, 163:175-247.
[3]
Brady CJ (1987). Fruit ripening. Ann Rev Plant Physiol., 38: 155-178.
[4]
Giovannoni JJ (2004). Genetic regulation of fruit development and ripening. Plant Cell, 16: S170-S180.
[5]
Peltier G, Cournac L (2002). Chlorespiration. Annu Rev Plant Biol, 53:523-550.
[6]
Carol P, Kuntz M (2001). A plastid terminal oxidase comes to light: implications for carotenoid biosynthesis and chlororespiration. Trends Plant Sci., 6: 31-36.
[7]
Pateraki I, Renato M, Azcón-Bieto J, Boronat A (2013). An ATP synthase harboring an atypical γ–subunit is involved in ATP synthesis in tomato fruit chromoplasts. The plant Journal, 74: 74-85.
[8]
Angaman DM, Petrizzo R, Hernandez-Gras F, Romero-Segura C, Pateraki I, Busquets M, Boronat A (2012). Precursor uptake assays and metabolic analyses in isolated tomato fruit chromoplasts. Plant Methods, 8(1):1.
[9]
Siedow JN, Girvin ME (1980). Alternative Respiratory Pathway: Its Role in Seed Respiration and Its Inhibition by Propyl Gallate, Plant Physiology, 65: 669-674.
[10]
Siedow JN, Bickett DM (1981). Structural features required for inhibition of cyanide-insensitive electron transfer by propyl gallate. Arch Biochem Biophys., 207(1):32-39.
[11]
Ferrie BJ, Beaudoin N, Burkhart W, Bowsher CG, Rothstein SJ (1994). The cloning of two tomato lipoxygenase genes and their differential expression during fruit ripening. Plant Physiol., 106(1):109-18.
[12]
Heitz T, Bergey DR, Ryan CA (1997). A gene encoding a chloroplast-targeted lipoxygenase in tomato leaves is transiently induced by wounding, systemin, and methyl jasmonate. Plant Physiology, 114:1085-1093.
[13]
Chen G, Hackett R, Walker D, Taylor A, Lin Z, Grierson D (2004). Identification of a specific isoform of tomato lipoxygenase (TomloxC) involved in the generation of fatty acid-derived flavor compounds Plant Physiology, 136: 2641–2651.
[14]
Morstadt L, Graber P, De Pascalis L, Kleinig H, Speth V, Beyer P (2002). Chemiosmotic ATP synthesis in photosynthetically inactive chromoplasts from Narcissus pseudonarcissus L. linked to a redox pathway potentially also involved in carotene desaturation. Planta, 215:134-136.
[15]
Veldink GA, Suurmeijer CNSP, Perez-Gilabert M, van der Hijden HTWM, Vliegenthart JFG (1998). Purification, product characterization and kinetic properties of soluble tomato lipoxygenase. Plant Physiol. Biochem. 36: 657-663.
[16]
Ealing PM (1994). Lipoxygenase activity in ripening tomato fruit pericarp tissue. Phytochem., 36: 547-552
[17]
Riley JCM, Willemot C, Thompson JE (1996). Lipoxygenase and hydroperoxide lyase activities in ripening tomato fruit. Postharvest Biol. Technol., 7: 97-107.
ADDRESS
Science Publishing Group
1 Rockefeller Plaza,
10th and 11th Floors,
New York, NY 10020
U.S.A.
Tel: (001)347-983-5186