Extraction and Purification of Flaxseed Proteins and Studying their Antibacterial Activities
Journal of Plant Sciences
Volume 2, Issue 1, February 2014, Pages: 70-76
Received: Dec. 25, 2013;
Published: Feb. 28, 2014
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Mohammad Hassan Houshdar Tehrani, Medicinal Chemistry Department, Shahid Beheshti University of Medical Sciences, Tehran, Iran; Phytochemistry Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Rumeysa Batal, Medicinal Chemistry Department, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Mohammad Kamalinejad, Pharmacognosy Department, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Arash Mahbubi, Pharmaceutics Department, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Flaxseed or linseed (Linum usitatissimum L.) is an annual herb belongs to the Linaceae family. It is cultivated worldwide and has been used for its oil seed and fiber since ancient times in Egypt, Rome and Greece. Nowadays it is considered as a medicinal plant in Asia, Europe and North America. Therapeutic effects of flaxseed fixed oil have been reported in several publications, but there are only few reports on biological activities of the flaxseed proteins. In the present study antimicrobial activities of the flaxseed proteins have been investigated. Hemogenized flaxseed flour was at first defatted and demucilaged, and its protein content was extracted in distilled water. After centrifugation, proteins of supernatant were isolated by adjusting pH. Gel filtration and ion-exchange chromatography on the protein extact gave fairly pure protein fractions, which antibacterial activities were examined against several microorganisms using the microbial micro-plate dilution method. The results showed that flaxseed proteins have an inhibitory activity on bacteria especially against Entrococcus foecalis, Salmonella typhimurium and Escherichia coli. Our experiment also revealed that mucilage composition as carbohydrates would not contribute in the inhibitory effect, but in fact, might cover and inhibit the antibacterial activity of flaxseed proteins. Moreover, it is possible that the carbohydrate components of flaxseed promote the microorganism growth.
Mohammad Hassan Houshdar Tehrani,
Extraction and Purification of Flaxseed Proteins and Studying their Antibacterial Activities, Journal of Plant Sciences.
Vol. 2, No. 1,
2014, pp. 70-76.
Pelegrini PB, Murad AM, Silva LP, dos Santos RCP, Costa FT, Tagliari PD, Bloch Jr C, Noronha EF, Miller RNG and Franco OL. Identification of a novel storage glycine-rich peptide from guava (Psidium guajava) seeds with activity against gram-negative bacteria. Peptides (2008) 29: 1271-1279.
Flores T, Alape-Giro A, Flores-Dı´az M, and Flores HE. Ocatin. A novel tuber storage protein from the Andean tuber crop Oca with antibacterial and antifungal activities. Plant Physiology (2002) 128: 1291–1302.
Tavares LS, Santos MdeO, Viccini LF, Moreira JS, Miller RNG and Franco OL. Biotechnological potential of antimicrobial peptides from flowers. Peptides (2008) 29: 1842-1851.
Houshdar Tehrani MH, Fakhrehoseini E, Kamali Nejad M, Mehregan H and Mojdeh Hakemi-Vala M. Search for proteins in the liquid extract of edible mushroom, Agaricusbisporus, and studying their antibacterial effects. Iranian Journal of Pharmaceutical Research (2002) 11: 145-150.
Wang S, Ng TB, Chen T, Lin D, Wu J, Rao P and Ye X. First report of a novel plant lysozyme with both antifungal and antibacterial activities. Biochemical and Biophysical Research Communications (2005) 327: 820–827.
Ribeiroa SFF, Carvalho AO, Cunha MD, Rodrigues R, Cruz LP, Melo VMM, Vasconcelos IM, Melo EJT and Gomes VM. Isolation and characterization of novel peptides from chilli pepper seeds: Antimicrobial activities against pathogenic yeasts. Toxicon (2007) 50: 600–611.
Lin P and Ng TB. A novel and exploitable antifungal peptide from kale (Brassica alboglabra) seeds. Peptides (2008) 29:1664-1671.
Rubilar M, Gutiérrez C, Verdugo M, Shene C and Sineiro J. Flaxseed as a source of functional ingredients. Journal of Soil Science and Plant Nutrition (2010) 10(3):373-377.
Anjum FM, Haider FM, Khan MI, Sohaib M and Arshad MS. Impact of extruded flaxseed meal supplemented diet on growth performance, oxidative stability and quality of broiler meat and meat products. Lipids in Health and Disease (2013) 12:13.
Gutiérrez C, Rubilar M, Jara C, Verdugo M, Sineiro J and Shene C. Flaxseed and Flaxseed cake as a source of compounds for food industry. Journal of Soil Science and Plant Nutrition (2010) 10 (4): 454 – 463.
Chung MWY, Lei B and Li-Chan ECY. Isolation and structural characterization of the major protein fraction from NorMan flaxseed (Linum usitatissimum L.). Food Chemistry (2005) 90: 271–279.
Marambe PWMLHK, Shand PJ and Wanasundara JPD. An In-vitro investigation of selected biological activities of hydrolysed Flaxseed (Linum usitatissimum L.) proteins. Journal of the American Oil Chemists' Society (2008) 85:1155–1164.
Sammour RH. Proteins of linseed (Linum usitatissimum L.), extraction and characterization by electrophoresis. Botanical Bulletin of Academia Sinica ( 1999) 40:121-126
Xu Y, Hall III C and Wolf-Hall C. Antifungal activity stability of Flaxseed protein extract using response surface methodology. Journal of Food Science (2008) 73(1):M9-M14.
Borgmeyer JR, Smith CE and Huynh OK. Isolation and characterization of 25 kDa antifungal protein from Flaxseeds. Biochemical and Biophysical Research Communications ( 1992) 187(1):480-87.
Kankaanpaa-Anttila B and Anttila M. Flax preparation, its use and production. United State Patent (1999) No.; 5,925,401.
Laemmli UK and Favre M. Gel electrophoresis of proteins. Journal of Molecular Biology (1973) 80: 575-599.
Bradford MM. Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding. Analytical Biochemistry (1976) 72:248-54.
Kumarasamy Y, Cox PJ, Jaspars M, Nahar L and Sarker SD. Screening seeds of Scottish plants for antibacterial activity. Journal of Ethnopharmacology (2002) 83: 73-77.
National Committee for Clinical Laboratory Standards (NCCLS). Methods for Dilution Antimicrobial Susceptibility Tests for Bacterial that Grow Aerobically. NCCLS Publication, wayne (2004) Document M7-A6.
Yang L and Zhang L. Chemical structural and chain conformational characterization of some bioactive polysaccharides isolated from natural sources. Carbohydrate Polymers (2009) 76, 349–361.
Smith AK, Johnsen VL and Beckel AC) Linseed proteins alkali dispersion and acid precipitation. Industrial & Engineering Chemistry (1946) 38(3):353–356.
Wanasundara PKJPD and Shahidi F. Removal of flaxseed mucilage by chemical and enzymatic treatments. Food Chemistry (1997) 59(1):47-55.
Hunt K and Jones JKN. The structure of linseed mucilage. Part II. Canadian Journal of Chemistry (1962) 40:1266-1279.
Guilloux K, Gaillard I, Courtois J, Courtois B and Petit, E. Production of Arabinoxylan-oligosaccharides from Flaxseed (Linum usitatissimum). Journal of Agricultural and Food Chemistry (2009) 57:11308-11313.