Screening of Phytochemicals, Antioxidant and Silver Nanoparticles Biosynthesizing Capacities of Some Medicinal Plants of Nepal
Journal of Plant Sciences
Volume 2, Issue 1, February 2014, Pages: 77-81
Received: Dec. 16, 2013;
Published: Feb. 28, 2014
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Khem Raj Giri, Department of Biochemistry, Universal Science College, Chakupat, Lalitpur, Nepal
Bibek GC, Department of Biochemistry, Universal Science College, Chakupat, Lalitpur, Nepal
Dipen Kharel, Department of Biochemistry, Universal Science College, Chakupat, Lalitpur, Nepal
Bimala Subba, Department of Chemistry, Tribhuvan University, Kirtipur, Kathmandu, Nepal
In this study, three plants i.e. J. adhatoda, A. vulgaris and P. guajava native to Pashupati area and Jorpati of Nepal were screened to evaluate the phytochemical and antioxidant activities due to their high medicinal value. The dried leaves were extracted with three solvents: hexane, methanol and distill water. The extracts were subjected to various phytochemical tests. The test confirmed the presence of various phytochemicals. The antioxidant activity of the methanol extracts were evaluated by FRAP assay. The antioxidant activity of J. adhatoda was found to be 0.794mM Fe (II)/L, A. vulgaris to be 0.949 mM Fe (II)/L and P. guajava was found to be 2.035 mM Fe (II)/L. Fresh aqueous leaf extracts of A. vulgaris and J. adhatoda were used for synthesis of silver nanoparticles. The biosynthesized AgNPs were characterized by Transmission Electron Microscope (TEM).
Khem Raj Giri,
Screening of Phytochemicals, Antioxidant and Silver Nanoparticles Biosynthesizing Capacities of Some Medicinal Plants of Nepal, Journal of Plant Sciences.
Vol. 2, No. 1,
2014, pp. 77-81.
Pamplona-Roger D. (1998). Encyclopedia of Medicinal Plants, Saeliz, Spain.
Polterait O. (1997). Antioxidants and free-radical scavengers of Natural Origin. Current Organic chemistry, 1: 415-440.
Akinmoladun AC, Ibukun EO, Afor E, Obuotor EM, Farombi EO. (2007). Phytochemical and antioxidant activity of extract from leaves of Ocimum gratissimum. Scientific Research and Essay, 2: 163-166.
Patel VK, Venkata-Krishna-Bhatt H. (1984). In vitro study of antimicrobial activity of Adhatoda vasica (Leaf extract) on gingival inflammation. A preliminary report. Indian Journal of Medical Science, 38: 70-72.
Karthikeyan A, Shanthi V, Nagasathya A. (2009). Preliminary phytochemical and antibacterial screening of crude extract of the leaf of Adhatoda vesica (L). International Journal of Green Pharmacy, 3: 78-80.
Kelsey RG, Shafizadeh F. (1979). Sesquiterpene lactones and systematics of the genus Artemisia. Phytochemistry, 18: 1591-1611.
Stuart M. (Editor). (1979). The Encyclopedia of Herbs and Herbalism Orbis Publishing. London, ISBN-o-85613-067-2.
Chiej R. Encyclopedia of Medicinal Plants. MacDonald (1984). ISBN 0-356-10541-5.
Lutterodt GD. (1992). Inhibition of Microlax-induced experimental diarrhea with narcotic-like extracts of Psidium guajava leaf in rats. Journal of Ethnopharmacol, 37(2):151–157.
Nair AGR, Subramanian SS. (1964). Variation in the chemical components of the stem-bark of Psidium guajava. Indian Journal of Pharmacy, 26: 140-1.
Arthur HR, Hui WH. (1954). Products from some plants of Hong Kong. Journal of Chemical Society, 2782.
Nair AGR, Subramanian SS. (1962). Chemical examination of the flowers of Eugenia Jambolana. Journal of Science and Industrial research, 21: 457-458.
KC SK, Klaus MJ. (1999). Medicinal plants from Nepal; II. Evaluation as inhibitors of liquid peroxidation in biological membranes. Journal of Ethnopharmacol, 64: 135-139.
Bhandari MR, Kawabata J.(2004). Organic acid, phenolic content and antioxidant activity of wild yam (Dioscorea spp.) tubers of Nepal. Food Chemistry, 88(2): 163-168.
Ciulei I. (1982). Methology for analysis of vegetable drugs, Practical manuals on industrial utilization of medicinal and aromatic plant, Bucharest, 73p.Phytochemistry, 63(1): 97-104.
Benzie I, Stain J. (1996). The Ferric reducing ability of plasma (FRAP) as measure of "Antioxidant Power": The FRAP assay. Analytical Biochemistry, 239: 70-76.
Shankar SS, Rai A, Ahmad A, Sastry M. (2004). Rapid synthesis of Au, Ag and bimetallic Au core-Ag shell nanoparticles using Neem (Azadirachta indica) leaf broth. Journal of Colloid Interface Science, 275:496-502.
Evans RCA, Miller NJ, Paganga G. (1996). Structure-antioxidant activity relationships of flavonoids and phenolic acids. Free Radical Biology and Medicine, 20: 933.
Tigno XT, Guzman de F, Ma A, Flora VT. (2000). Phytochemical analysis and hemodynamic actions of Artemisia vulgaris L.Clinical Hemorheology and Microcirculation, 23(2):167-175.
Ayoola GA, Coker HAB, Adesegun SA, Adepoju-Bello AA, Obaweya K, Ezennia, Atangbayila TO. (2008). Phytochemical screening and antioxidant activities of some selected medicinal plants used for Malaria therapy in southwestern Nigeria.Tropical Journal of Pharmaceutical Research, 7(3):1019-1024.
Walter C, Shinwari ZK, Afzai I, Malik RN. (2011). Antibacterial activity in herbal products used in Pakistan. Pakistan Journal of Botany, 43: 155-162.
M Ip, Liu SL, Poon VK, Lung I, Burd A. (2006). Antimicrobial activities of silver dressings: an in vitro comparison. Journal of Medicinal Microbiology, 55:59-63.
Zhu JJ, Liao XH, Zhao XN, Chen HY. (2001). Preparation of silver nanorods by electrochemical methods. Materials Letters, 49: 91-95.
Liu S, Huang W, Chen S, Avivi S, Gendanken A. (2001). Synthesis of X-ray amorphous silver nanoparticles by the pulse sonoelectrochemical method. Journal of Non-Cryst Solids, 283: 231-236.
Chou WL, Yu DG, Yang MC. (2005). The preparation and characterization of silver-loading cellulose acetate hollow fiber membrane for water treatment. Polymer advanced technology, 16: 600-607.
Vilchis-Nestor AR, Sanchez-Mendieta V, Camacho-Lopez MA, Gomez- Espinosa RM, Camacho Lopez MA, Arenas- Alatorre JA. (2008). Solventless synthesis and optical properties of Au and Ag nanoparticles using Camellia sinensis extract. Materials Letters, 62:3103-3105.
Abu Bakarj Ismail NHH, Abu Bakar M. (2004). Synthesis and characterization of silver nanoparticles in natural rubber. Mater Chem Phys104:276-283.
Chandran SP, Chaudhary M, Pasriocha R, Ahmad A, Sastry M. (2004). Synthesis of gold nanotriangles and silver nanoparticles using Aloe vera plant extract. Biotechnology Progress, 22: 577-583.
Vigneshwaram N, Nachane RP, Balasubramanya RH, Varadrajan PV. (2006). A novel one pot "Green synthesis of stable silver nano particles using soluble starch. Carbohydrate Research, 341:2012-1218.