Leghaemoglobin Sub-Fractional Components in Chickpea Root Nodules during Extended Darkness
Journal of Plant Sciences
Volume 2, Issue 4, August 2014, Pages: 134-138
Received: Aug. 6, 2014; Accepted: Aug. 13, 2014; Published: Aug. 30, 2014
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Kamal Jit Singh, Department of Botany, Panjab University, Chandigarh 160 014, India
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The aim of study was to investigate qualitative behavior of leghaemoglobin sub-fractional components during dark induced nodular senescence. A conventional protein purification method using ion exchange chromatography (HPLC) readily resolved ferric Lb into eight sub-fractional components namely a1, a2; b; c1, c2 and d1, d2 d3 in the unstressed chickpea nodules. Lb complexes behave differently during growth phases of the nodules. Lb ‘a’ complex is directly related to the growth and developmental of nodules wherein proportion of Lb a2 content increases with age of nodule accompanying concurrent decrease Lb a1. Early appearance of senescence related isoprotein Lb a2 at vegetative phase of chickpea cultivar correlates its stress-susceptible nature. Further, the turnover rates of Lb a1 to a2 and Lb b were insensitive to reduced supply of photosynthesis during dark stress and even re-illumination. The relative proportion of c2 to c1 inversion increases during darkness. Further, Lb‘d’ complex is affected the most during prolonged darkness. Thus, ratio between individual sub-fractional components of Lbs’ can be correlated with the development phase, longevity and supply of carbohydrates to nodules.
Components, Dark Stress, Ion-Exchange, Sucrose
To cite this article
Kamal Jit Singh, Leghaemoglobin Sub-Fractional Components in Chickpea Root Nodules during Extended Darkness, Journal of Plant Sciences. Vol. 2, No. 4, 2014, pp. 134-138. doi: 10.11648/j.jps.20140204.13
Appleby C.A., 1992. The origin and functions of haemoglobin in plants. Sci. Progress Oxford. Vol. 76: 365-398.
Becana M., Sprent J.I., 1989. Effect of nitrate on components of nodule leghaemoglobins. Journal of Experimental Botany. Vol. 40(216): 725-731.
Becana M., Aparicio-Tejo P., Pena J., Aguirredea J., Sanchez-Diaz M., 1986. N2 fixation and leghaeoglobin content during nitrate and water stress induced senescence of Medicago sativa root nodules. Journal of Experimental Botany. Vol. 37: 597-605.
Bisseling T., Vanden Bos R.C., Kammen V.A., 1978. The effect of ammonium nitrate on the synthesis of nitrogenase and the concentration of leghaemoglobin in pea root nodules induced by Rhizobium leguminosarum. Biochimica Biophysica Acta. Vol. 539: 1-11.
Dakora F.D., Appleby C.A., Atkins C.A., 1991. Effect of pO2 on the formation and status of leghemoglobin. Plant Physiology. Vol. 95: 723-730.
Fuchsman W.H., 1992. Plant hemoglobins. Advances in Computers and Environmental Physiology. Vol. 13: 23-58.
Fuchsman W.H., Appleby C.A., 1979a. Separation and determination of the relative concentrations of the homogenous components of soybean leghaemoglobin by isoelectric focusing. Biochimica Biophysica Acta. Vol. 579: 314-324.
Fuchsman W.H., Appleby C.A., 1979b. CO and CO2 complexes of soybean leghaemoglobin: pH effects upon infrared and visible spectra. Comparison with CO and O2 complexes of myoglobin and haemoglobin. Biochemistry. Vol. 18: 1309-1321.
Marino D., Damiani I., Gucciardo, S., Mijangos I., Pauly N., Puppo A., 2013. Inhibition of nitrogen fixation in symbiotic Medicago truncatula upon Cd exposure is a local process involving leghaemoglobin. Journal of Experimental Botany. Vol. 64(18): 5651-5660.
Mendonca E.H.M., Mazzafera P., Schiavinato M.A., 1999. Purification of leghemoglobin from nodules of Crotolaria infected with Rhizobium. Phytochemistry. Vol. 50: 313-316.
Muneer S., Ahmad J., Bashir H., Qureshi M.I., 2012. Proteomics of nitrogen fixing nodules under various environmental stresses. Plant Omics Journal. Vol. 5(2): 167-176.
O’Brian M.R., Kirshbom P.M., Maier R.J., 1987. Bacterial heme synthesis is required for expression of the leghemoglobin holoprotein but not the apoprotein in soybean root nodules. Proceedings National Academy of Sciences. Vol. 84(23): 8390-8393.
Rao L., 1991. Nodulation and regulation of nitrogen fixation in relation to saline conditions in some important leguminous crop plants. Ph.D. dissertation, Panjab University, Chandigarh, India.
Roponen, I., 1970. The effect of darkness on the leghaemoglobin content and amino acid levels in the rot nodules of pea plants. Physiologia Plantarum. Vol. 23: 452-460.
Saari L.L., Martin K.D., Guang-xin W., Wang T., Pankhurst L.J., Klucas R.V., 1988. Oxygen, carbon monoxide, azide binding to the eight components of soybean leghaemoglobin. In: Bothe H., de Bruijn F.J., Newton W.E. (eds.), Nitrogen Fixation: hundreds years after. Gustav Fischer, Stuttgart. pp. 642.
Santana M.A., Pihakaski-Maunsbach K., Sandal N., Marcker K.A., Smith A.G., 1998. Evidence that the plant host synthesizes the heme moiety of leghemoglobin in root nodules. Plant Physiology. Vol. 116: 1259-1269.
Sarath G., Cohen H.P., Wagner F.W., 1986. High-performance liquid chromatographic separation of leghaemoglobins from soybean root nodules. Annals of Biochemistry. Vol. 154: 224-231.
Shleev S.V., Rozov F.N., Topunov A.F., 2001. A method for producing multiple forms of metleghemoglobin reductase and leghemoglobin components from lupine nodules. Applied Biochemistry and Microbiology. Vol. 37(2):195-200.
Singh K.J., 1994. Physiological and biochemical studies on the effect of salt, dark and water stresses on nodulation and regulation of nitrogen fixation in some important legume crops. Ph.D. dissertation submitted to Panjab University, Chandigarh, India.
Sprent J.I., 1976. Nitrogen fixation by legumes subjected to water and light stresses. In: Nutman P.S., (ed.), Symbiotic nitrogen fixation in plants. Cambridge University Press. pp. 405-420.
Swaraj K., Garg O.P., 1977. The effect of ageing on the leghaemoglobin of cowpea nodules. Physiologia Plantarum. Vol. 39: 185-189.
Szybiak-Strozycka U., Strozycki P., Sikorski M., Goinska B., Madrzak C., Legocki, A.B., 1987. Lupin leghaemoglobin during root nodule development. Acta Biochemistry Polonica. Vol. 34: 79-85.
Uheda E., Syono K., 1982. Effects of leghaemoglobin components on nitrogen fixation and oxygen consumption. Plant Cell Physiology. Vol. 23: 85-90.
Ulrich K., Lentzsch P., Seyfarth W., 1997. Identification of cultivar-specific leghaemoglobin components in Pisum sativum. New Phytol. Vol. 137: 285-291.
Verma D.P.S., Ball S., Guerin C., Wanamaker L., 1979. Leghaemoglobin biosynthesis in soybean root nodules. Characterization of the nascent and released peptides and the relative rate of the minor leghaemoglobin. Biochemistry. Vol. 18: 476-483.
Wittenberg J.B., Appleby C.A., Wittenberg B.A., 1972. The kinetics of the relations of leghaemoglobin with oxygen and carbon monoxide. Journal of Biological Chemistry. Vol. 247: 527-531.
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