Identification of Leaf Rust Resistance Genes in Egyptian Wheat Cultivars by Multipathotypes and Molecular Markers
Journal of Plant Sciences
Volume 2, Issue 5, October 2014, Pages: 145-151
Received: Jul. 10, 2014;
Accepted: Jul. 30, 2014;
Published: Sep. 20, 2014
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Mohammed Saad Abou-Elseoud, Department of Plant Pathology, Faculty of Agriculture, Alexandria University, El-Shatby 21545, Alexandria, Egypt
Abd-Elmageed Mohammed Kamara, Department of Plant Pathology, Faculty of Agriculture, Alexandria University, El-Shatby 21545, Alexandria, Egypt
Omaima Abd-Ellatif Alaa-Eldein, Wheat Diseases Research Department, Plant Pathology Research Institute, PPRI, Agricultural Research Centre, Giza 12619, Egypt
Ahmed Farag El-Bebany, Department of Plant Pathology, Faculty of Agriculture, Alexandria University, El-Shatby 21545, Alexandria, Egypt
Nader Abd-Elwahab Ashmawy, Department of Plant Pathology, Faculty of Agriculture, Alexandria University, El-Shatby 21545, Alexandria, Egypt
Ibrahim Sobhy Draz, Wheat Diseases Research Department, Plant Pathology Research Institute, PPRI, Agricultural Research Centre, Giza 12619, Egypt
A total of twenty leaf rust resistance genes (Lr genes) were postulated in nine Egyptian wheat cultivars based on infection types (ITs) expressed on the tested cultivars by 72 Puccinia triticina pathotypes compared with the ITs expressed on the monogenic lines. The most carrier genes cultivars were Giza168 and Misr1 each may contain five genes i.e. Lr2c, 10, 18, 24, 41 and Lr3, 10, 19, 22b, 24, respectively. Five cultivars, Sakha94, Gemmeiza9, Gemmeiza10, Sids12 and Misr2 each probably contain four genes i.e. Lr9, 19, 29, 37; Lr18, 21, 24, 41; Lr3, 9, 19, 29; Lr9, 19, 26, 29 and Lr3, 10, 19, 26, respectively. Gemmeiza11 was the least cultivar carrying genes; it probably carries just two genes i.e. Lr24 and Lr41. The most postulated genes were Lr19 and Lr24, each postulated within five cultivars followed by Lr41 within four cultivars. Five Lr genes, Lr3, Lr9, Lr10, Lr26 and Lr29 each within three cultivars. The lowest postulated genes were Lr2c, Lr21, Lr22b and Lr37 each of them was postulated within only one cultivar. Five Lr genes, Lr9, Lr10, Lr19, Lr24 and Lr26 were identified by PCR-based molecular marker. The Lr9 gene was identified in cultivar Sids12 while, Lr10 was identified in cultivar Misr1. The Lr19 was present in two cultivars, Misr1 and Misr2. The Lr24 and Lr26 were absent in all the screened Egyptian cultivars. The obtained results for Lr9, Lr10, Lr19, Lr24 and Lr26 marker were in agreement with and confirm their identification by gene postulation. Markers for Lr9, Lr10 and Lr19 may be useful in marker-assisted breeding. Our findings showed the usefulness of the molecular marker in identifying leaf rust resistance genes in wheat cultivars, especially when used in conjunction with multipathotypes test at the pre-breeding stage. This approach may help understanding the wheat - P. triticina interaction and provide information to build an effective management program for leaf rust disease.
Mohammed Saad Abou-Elseoud,
Abd-Elmageed Mohammed Kamara,
Omaima Abd-Ellatif Alaa-Eldein,
Ahmed Farag El-Bebany,
Nader Abd-Elwahab Ashmawy,
Ibrahim Sobhy Draz,
Identification of Leaf Rust Resistance Genes in Egyptian Wheat Cultivars by Multipathotypes and Molecular Markers, Journal of Plant Sciences.
Vol. 2, No. 5,
2014, pp. 145-151.
FAO, “Production prospects for 2013 cereal crops,” Global Information and Early Warning System (GIEWS) report, 2013, p. 4.
J. Huerta-Espino, R. P. Singh, S. German, B. D. McCallum, R. F. Park, W. Q. Chen, S. C. Bhardwaj and H. Goyeau, “Global status of wheat leaf rust caused by Puccinia triticina,” Euphytica, vol. 179, pp. 143-160, 2011.
E. E. Saari and J. M. Prescott, “World distribution in relation to economic losses,” In, Roelfs AP, Bushnell WR (eds) The Cereal Rusts, vol II. Academic Press, Orlando, FL, 1985, pp. 259-298.
A. P. Roelfs, R. P. Singh, and E. E. Saari, “Rust Diseases of Wheat: Concepts and methods of disease management,” Mexico, D.F.: CIMMYT, 1992, p. 81.
C. N. Marasas, M. Smale, and Singh, R. P. “The Economic Impact in Developing Countries of Leaf Rust Resistance Breeding in CIMMYT-related Spring Bread Wheat,” Economics Program Paper 04-01, Mexico, D.F.: CIMMYT, 2004.
J. A. Kolmer, D. L. Long and M. E. Hughes, “Physiological specialization of Puccinia triticina on wheat in the United States in 2003,” Plant Dis., vol. 89, pp. 1201-1206, 2005.
T. M. Abdel-Hak, N. A. EL-Sherif, A. A. Bassiouny, I. I. Shafik and Y. H. EL-Dauadi, “Control of wheat leaf rust by systemic fungicides,” Proceedings of the Fifth European and Mediterranean Cereal Rusts Conference, Bari, Italy, 1980, pp. 255-266.
M. Nazim, A. A. El-Shehidi, Y. A. Abdou and Y. H. El-Daoudi, “Yield loss caused by leaf rust on four wheat cultivars under epiphytotic levels,” 4th Conf. Microbiol., Cairo, 1983, pp. 17-27.
R. A. McIntosh, Y. Yamazaki, K. M., Devos, J. Dubcovsky, J. Rogers and R. Appels, “Catalogue of gene symbols for wheat,” 2007 Supplement, KOMUGI Integrated Wheat Science Database.
L. Stepien, L. Golka and J. Chelkowski, “Leaf rust resistance genes of wheat. identification in cultivars and resistance sources,” J. Appl. Genet., vol. 44, pp. 139-149, 2003.
L. E. Browder and M. G. Eversmeyer, “Sorting of Puccinia recondita: Triticum infection-type data sets toward the gene-for-gene model,” Phytopathol, vol. 70, pp. 666-670, 1980.
G. D. Statler, “Probable genes for leaf rust resistance in several hard red spring wheats,” Crop Sci., vol. 14, pp. 883-886, 1984.
E. C. Stakman, D. M. Stewart and W. Q. Loegering, “Identification of physiologic races of Puccinia graminis tritici,” ARS, USDA, Agric. Res. Serv. Bull. E-617, 1962, p. 53.
H. W. Ohm and G. E. Shaner, “Three components of slow leaf-rusting at different growth stages in wheat,” Phytopathol., vol. 66, pp. 1356-1360, 1976.
C. O. Johnston and L. E. Browder, “Seventh revision of the international register of physiologic races of Puccinia recondita f. sp. tritici,” Plant Dis. Reporter, vol. 50, pp. 756-760, 1966.
F. M. Doohan, D. W. Parry, P. Jenkinson and P. Nicholson “The use of species-specific PCR-based assays to analyse Fusarium ear blight of wheat,” Plant Pathol., vol. 47, pp. 197-205, 1998.
G. Schachermayr, H. Siedler, M. D. Gale, H. Winzeler, M. Winzeler and B. Keller, “Identification and localization of molecular marker linked to the Lr 9 leaf rust resistance gene of wheat,” Thero. Appl. Genet., vol. 88 (1), pp. 110-115,1994.
G. Schachermayr, C. Feuilletand and B. Keller, “Molecular marker for the detection of the wheat leaf rust resistance gene Lr10 in diverse genetic backgrounds,” Mol. Breed., vol. 3 (1), pp. 65-74, 1997.
R. Prins, J. Z. Groenewald, G. F. Marais, J. W. Snape and R. M. D. Koebner, “AFLP and STS tagging of Lr19, a gene conferring resistance to leaf rust in wheat,” Theor. Appl. Genet., vol. 103, pp. 618-624, 2001.
G. M. Schachermayr, M. M. Messmer, C. Feuillet, H. Winzeler, M. Winzeler and B. Keller, “Identification of molecular marker linked to the Agropyron elongatum derived leaf rust resistance gene Lr24 in wheat,” Theor. Appl. Genet., vol. 90, pp. 982-990, 1995.
C. Person, “Gene-for-gene relation ships in host . parasite systems,” Can. J. Bot., vol. 37, pp. 1101-1130, 1959.
H. H. Flor, “Current status of the gene-for-gene concept,” Annu. Rev. Phytopathol., vol. 9, pp. 275-296, 1971.
W. Q. Loegering, “Genetics of the pathogen-host association,” The Cereal Rusts. Orlando, FL. Academic, vol I, 1985, pp. 169-192.
D. V. McVey, “Verification of infection type data for identification of genes for resistance to leaf rust in some hard red spring wheats,” Crop Sci., vol. 29, pp. 304-307, 1989.
R. P. Singh and S. Rajaram, “Resistance to Puccinia recondita f. sp. tritici in 50 Mexican bread wheat cultivars,” Crop Sci., vol. 31, pp. 1472-1479, 1991.
R. P. Singh and A. K. Gupta, “Genes for leaf rust resistance in Indian and Pakistan wheats tested with Mexican pathotypes of Puccinia recondita f. sp. tritici,” Euphytica, vol. 57, pp. 27-36, 1991.
R. P. Singh, “Resistance to leaf rust in 26 Mexican wheat cultivars,” Crop Sci., vol. 33, pp. 633-637, 1993.
D. Singh, R. F. Park and R. A. McIntosh, “Genetics relationship between the adult plant resistance gene Lr12 and the complementary gene Lr31 for seedling resistance to leaf rust in common wheat,” Plant Pathol., vol. 48, pp. 567-573, 1999.
D. V. McVey, M. Nazim, and K. J. Leonard, “Postulated genes for resistance to leaf rust of 25 Egyptian bread and six durum wheat cultivars,” Cereal Rusts and Powdery Mildews Bulletin., 2004, pp. 1-9,
I. A. Imbaby, “Use of infection type data to identify genes for low reaction to wheat leaf rust in Gemmeiza and Sids cultivars,” Egypt. J. Phytopathol., vol. 35 (1), pp. 25-34, 2007.
R. P. Singh and R. A. McIntosh, “Complementary genes for reaction to Puccinia recondita f. sp. tritici in Triticum aestivum genetic and linkage studies,” Can. J. Genet. Cytol., vol. 26, pp. 723-725, 1984.
M. A. Najeeb, O. A. Boulot, M. M. Musa and S. S. Negm, “Physiological specialization in Puccinia triticina and postulated genes of resistance in certain Egyptian wheat cultivars,” Annals of Agric. Sci. Moshtohor, vol. 43, pp. 265-278, 2005.
J. A. Kolmer, and M. E. Ordoñez, “Differentiation of molecular genotypes and virulence phenotypes of Puccinia triticina from common wheat in North America,” Phytopathol., vol. 99, pp. 750-758, 2007.
Z. F. Li, X. C. Xia, Z. H. He, X. Li, L. J. Zhang, H. Y. Wang, Q. F. Meng, W. X. Yang, G. Q. Li, and D. Q. Liu, “Seedling and slow rusting resistance to leaf rust in Chinese wheat cultivars,” Plant Dis., vol. 94 (1), pp. 45-53, 2010.
Y. Sun, Y. Y. Hu, W. X. Yang and D. Q. Liu, “Evaluation of the resistance to leaf rust of 6 wheat lines,” J. Tritic. Crops, vol 31, pp. 762-768, 2011.
A. Abdelbacki, N. Soliman, M. Najeeb, and R. Omara, “Postulation and identification of resistance genes against Puccinia triticina in new wheat cultivars in Egypt using molecular marker,” International Journal of Chemical, Environmental & Biological Sciences (IJCEBS), vol. 1 (1), pp. 2320-4087, 2013.