Biological Control of Root Knot Nematodes in Chillies through Pseudomonas fluorescens’s Antagonistic Mechanism
Journal of Plant Sciences
Volume 2, Issue 5, October 2014, Pages: 152-158
Received: Sep. 5, 2014; Accepted: Sep. 18, 2014; Published: Sep. 30, 2014
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Authors
Samaraj Subramanian Thiyagarajan, R&D, Hartley Biocontrol, Coimbatore, 641045, TamilNadu, India; University of Texas at San Antanio, D Current institution Thermo Fischer Scientific; Sugarcane Breeding Institue, Coimbatore, 641007, TamilNadu, India
Hari Kuppusamy, R&D, Hartley Biocontrol, Coimbatore, 641045, TamilNadu, India
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Abstract
Among many pests and diseases, nematodes are one of the most important pests. Managing nematode chemically is very expensive. The damage caused by this pest results in considerable economical loss for the agriculture community. The important factor for increased pest management expenses, and decreased return on investment is due to inefficiency of chemical nematicides to provide a prolonged pest resistance. Chilli (Capsicum annum) is an important crop in India and is cultivated throughout India. Nematodes are an important pest for chillies. Primary nematode species infesting chili plants are the Root knot nematode Meloidogyne Incognita. In this study, susceptible variety PKM-1 developed by Tamil Nadu Agriculture University was used. Nematode control through biopesticides Pseudomonas fluorescence was assessed. Pseudomonas fluorescences’s antagonistic activity was studied to understand the efficiency and duration of its antagonistic activity. Pseudomonas fluorescens’s nematode resistance was compared with chemical and untreated plants. In summary, the study concludes that Pseudomonas fluorescens comparatively was more effective in sustained control of nematodes than chemical treatments.
Keywords
Pseudomonas Fluorescens, Root Knot Nematode, Carbofuran. Chilli Capsicum Annum
To cite this article
Samaraj Subramanian Thiyagarajan, Hari Kuppusamy, Biological Control of Root Knot Nematodes in Chillies through Pseudomonas fluorescens’s Antagonistic Mechanism, Journal of Plant Sciences. Vol. 2, No. 5, 2014, pp. 152-158. doi: 10.11648/j.jps.20140205.12
References
[1]
Bird A.F, “The Inducement of Giant Cells by Meloidogyne Javanica,” Nematologica, vol. 8, pp. 8–10, 1960.
[2]
Bird. A.F, “Changes associated with parasitism in nematodes. IV. Cytochemical studies on the ampulla of the dorsal esophageal gland of Meloidogyne javanica and on exudations from the buccal stylet.” J. Parasitol., vol. 54, pp. 879–890, 1968.
[3]
Alam M.M., Siddiqi Z.A., Khan A.M., Saxena S.K., “Effect of different crop sequences on the population of plant para¬sitic nematodes.,” . Indian J. Nematol., vol. 10(1), pp. 35–39, 1980.
[4]
Van Peer, R. and B. Schippers., “Lipopolysaccharides of plant growth-promoting Pseudomonas sp. strain WCS417r induce resistance in carnation to Fusarium wilt. Neth.,” Plant Pathol., vol. 98, pp. 129–139, 1992.
[5]
Becker, J. O., E. Zavaleta-Mejia, S. F. Colbert, M. N. Schroth, A. R. Weinhold, J.G. Hancock, and S. D. Van Gundy.. Effects of rhizobacteria on root-knot nematodes and gall formation. Phytopathology 78:1466–1469, 1988
[6]
A.F. Bird, “The attractiveness of roots to the plant parasitic nematodes Meloidogyne javanica and M. hapla,” Nematologica, vol. 4, pp. 322–335, 1959.
[7]
A. F. Bird., “Inducement of Giant Cells by Meloidogyne Javanica,” Nematologica, vol. 1962, pp. 1–10, 1962.
[8]
Kerry. B.R, “Rhizosphere interactions and the exploitation of microbial agents for the biological control of plant-parasitic nematodes,” Phytopathology, vol. 38, pp. 423–441, 2000.
[9]
CT Bull, DM Weller, LS Thomas, “How relationship between root colonization and suppression of Gaeumannomyces graminis var. tritici by Pseudomonas fluorescens strain,” Phytopathology, vol. 81, pp. 954–959, 1991.
[10]
Keel. CJ, “Bacterial antagonists of plant pathogens in the rhizosphere: Mechanisms and prospects,” OIL/SROP, vol. xv, pp. 93–99, 1992.
[11]
D.J. PREE, J. L. TOWNSHEND, AND K. J. COLE, “Inhibition of Acetylcholinesterases from Aphelenchus avenae by Carbofuran and Fenamiphos,” J. Phytopathol., vol. 22(2), pp. 182–186, 1990.
[12]
Jonathan E. I, K. R. Barker, F. F. Abdel-Alim, T. C. Vrain, and D. W. Dickson, “Biological control of Meloidogyne incognita on tomato and banana with rhizobacteria, actinomycetes, and Pasteuria penetrans.,” Nematropica, vol. 30, pp. 231–240, 2000.
[13]
Nordmeyer D. and Dickson DW., “Effect of carbamate, organophosphate and Avermectin nematicides on oxygen consumption by three Meloidogyne spp.,” nematology, vol. 21(4), pp. 472–476, 1989.
[14]
Anita E and Rajendran G, “Nursery application of Pseudomonas fluorescens for the control of meloidogyne incognita on tomato and brinjal,” Nematol. Mediterr., vol. 30, pp. 209–210, 2002.
[15]
Duffy, B. K., and Défago, “Zinc improves biocontrol of Fusarium crown and root rot of tomato by Pseudomonas fluorescens and represses the production of pathogen metabolites inhibitory to bacterial antibiotic biosynthesis,” Phytopathology, vol. 87, pp. 1250–1257, 1997.
[16]
F. Griffin, D. M., Hale, M. G., and Shay, “Nature and quantity of sloughed organic matter produced by roots of axenic peanut plants,” SOIL BIOL.BIOCHEM, vol. 8, pp. 29–32, 1976.
[17]
Gamliel, A., and J. Katan, “Involvement of fluorescent pseudomonads and other microorganisms in increased growth response of plants in solarized soils,” Phytopathology, vol. 81, pp. 494–502, 1991.
[18]
Giebel. J, “Biochemical mechanisms of plant resistance to nematodes,” Nematologica, pp. 175–181, 1974.
[19]
Schroth, M. N. and Hancock, J. G.., “Disease-suppressive soil and root-colonizing bacteria.” Science (80-. )., vol. 216, pp. 1376–1381, 1982.
[20]
D. D. Jonathan EI, Barker KR, Abdel-Alim FF, Vrain TC, “Biological control of Meloidogyne incognita on tomato and banana with rhizobacteria, actinomycetes, and Pasteuria penetrans,” Nematropica, vol. 30, pp. 231–240, 2000
[21]
S. R. Kandan A, Radjacommare R, Nandakumar R, Raguchander T, Ramiah M, “ Induction of phenylpropanoid metabolism by Pseudomonas fluorescens against tomato spotted wilt virus in tomato,” F MICROBIOL, vol. 47, pp. 121–129, 2002.
[22]
L. B. Kloeppe DA, Nyczepir AP, Lawrence JE, “Biological Control of the Phytoparasitic Nematode Mesocriconema xenoplax on Peach Trees,” nematology, vol. 34(2), pp. 120–123, 2002.
[23]
Barker. KR, “Nematode extraction and bioassays,” Methodology, vol. 2, pp. 4–5, 1985.
[24]
Raguchander. T. and S. R. Nandakumar R, Babu S, Viswanathan R, “Induction of systemic resistance in rice against sheath blight disease by Pseudomonas fluorescens,” SOIL BIOL.BIOCHEM, vol. 33(4), pp. 603–612, 2000.
[25]
Sheela. J. Raguchander. T. and Samiyappan. R. Nandakumar R, Babu S, Viswanathan S, “A new bio-formulation containing plant growth promoting rhizobacterial mixture for the management of sheath blight and enhanced grain yield in rice,” BIOCONTROL, vol. 46, pp. 493–510, 2001.
[26]
Nusbaum C.J., Ferris H., “The role of cropping systems in nematode population management.,” Ann. Rev. Phytopa¬thology, vol. 11, pp. 423–440, 1973.
[27]
L. S. Pierson., “. Expanding the club: engineering plants to talk to bacteria.,” . Trends Plant Sci., vol. 5, pp. 89–91, 2000.
[28]
JW Kloeppes ., R Rodriguez-Kabana., “Plant root-bacterial interactions in biological control of soil borne diseases and potential extension to systemic and foliar diseases,” Australas. Pathol., vol. 28, pp. 21–26, 1999.
[29]
Sikora. RA., “Interrelationship between plant health promoting rhizobacteria, plant parasitic nematodes and soil microorganisms.,” . Meded. Fac. Landb, Rijksuniv Gen, vol. 53, pp. 867–878, 1988.
[30]
Cook. RJ, “Making greater use of introduced microorganisms for biological control of plant pathogens.,” Phytopathology, vol. 31, pp. 53–80, 1993.
[31]
Hussey. RS, “Disease-inducing secretions of plant-parasitic nematodes.,” Annu. Rev. Phytopathol., vol. 27, pp. 123–141, 1989.
[32]
1. Siddiqui IA and Ehteshamul-Haque S, “Use of Pseudomonas fluorescens aeruginosa for the control of root rot-root knot disease complex in tomato,” Plant soil, vol. 227, pp. 163–169, 2000.
[33]
Robin Duponnois., Amadou M. BA and Thierry Mateille., “ Effects of sorne rhizosphere bacteria for the biocontrol of nematodes of the genus, Meloidogyne with Arthrobotrys oligospora,” fF A Nemawl, vol. 21(2), pp. 157–163, 1998.
[34]
Sitaramaiah K.,. Plant Parasitic Nematodes of India. Today and Tomorrow. Printers and Publishers, New Delhi: 292, 1984
[35]
M. I. Siddiqui ZA, “Role of bacteria in the management of plant parasitic nematodes: Bioresource technology.,” A Rev., 69AD.
[36]
Singh, P.P, Y.C. Shin, C.S. Park and Y.R. Chung,, “Biological control of Fusarium wilts of cucumber by chitinolytic bacteria.,” Phytopathology, vol. 89, no. 92–99, 1999.
[37]
J. Handlesman and E. V. Stabb, “Biocontrol of Soil borne Plant Pathogens,” Plant Cell, vol. 8, pp. 1855–1869, 1966.
[38]
Nandakumar R, Babu S, Viswanathan R, Raguchander T and Samiyappan R. Induction of systemic resistance in rice against sheath blight disease by Pseudomonas fluorescens. Soil Biol. Biochem 33 (4), 603-612 , 2000.
[39]
Vidhyasekaran, P., R. Rabindran, M. Muthamilan, K. Nayar, K. Rajappan, N. Subramanian and K. Vasumathi, “Development of powder formulation of Pseudomonas fluorescens for control of rice blast.,” Plant Pathol., vol. 46, pp. 291–297, 1997.
[40]
lsgouhi Kaloshian Valerie M. Williamson Gene Miyao Dennis A. Lawn Becky Westerdahl, “Resistance-breaking nematodes identified in California tomatoes,” Calif. Agric., vol. 50, pp. 6–7, 1995.
[41]
Windhum GL, Windhum MT & Williams WP, “Plant Effect of Trichoderma species on maize growth and Meloidogyne,” pp. 701–15, 1989.
[42]
H. C. D. Yeole, R. D., B. P. Dave, “‘Siderophore production by fluorescent pseudomonads colonizing roots of certain crop plants.,’” ." Indian J. Exp. Biol., vol. 39, pp. 464–468, 2001.
[43]
M. C. Zhao, X., Schmitt, M., and Hawes, “Species-dependent effects of border cell and root tip exudates on nematode behavior,” Phytopathology, vol. 90, pp. 1239–1245, 2000.
[44]
R. Nandakumar, S. Babu, R. Viswanathan, J. Sheela T. Raguchander And R. Samiyappan A new bio-formulation containing plant growth promoting rhizobacterial mixture for the management of sheath blight and enhanced grain yield in rice, BioControl 46: 493–510, 2001.
[45]
J Giebel, Biochemical mechanisms of plant resistance to nematodes: A review, - Journal of nematology, 1974
[46]
Samaraj S. Thiyagarajan, and K. Hari, Tomato Root Knot Nematode Control through Biocontrol Agent Pseudomonas fluorescens. International Journal of Research in Agricultural Sciences, Volume 1, Issue 4, ISSN(Online):2348–3997, 2014.
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