Effect of the Two Antioxidants; Glutathione and Ascorbic Acid on Vegetative Growth, Yield and Some Biochemical Changes in Two Wheat Cultivars
Journal of Plant Sciences
Volume 2, Issue 5, October 2014, Pages: 215-221
Received: Oct. 1, 2014;
Accepted: Oct. 13, 2014;
Published: Oct. 30, 2014
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Mohamed El-Sayed El-Awadi, Botany Department, National Research Centre, Dokki, Giza, Egypt
Safaa Reda El-Lethy, Botany Department, National Research Centre, Dokki, Giza, Egypt
Kawther Gad El-Rokiek, Botany Department, National Research Centre, Dokki, Giza, Egypt
Two field experiments were carried out in the experimental farm of National Research Center at Nubaria, Egypt, during two successive seasons 2011/2012 and 2012/2013 to study the effect of the two antioxidants; glutathione and ascorbic acid on growth, yield and yield components of two cultivars of wheat plant (Triticum aestivum L.). The two cultivars were Sakha93 and Giza168. The two antioxidants were foliarly applied twice at the two concentrations 50 and 100ppm for each. The first spray was applied 30 days after sowing and the second spray was applied 15 days later (45 days after sowing). The results indicated significant increases in the growth of the two wheat cultivars Sakha93 and Giza168. The increase in growth was accompanied by increase in yield and yield components of the two cultivars compared to the corresponding controls. In general, growth and yield exhibited greatest response towards the higher concentration of glutathione. The increase in wheat growth was concomitant with increases in photosynthetic pigments, carbohydrate, total free amino acids and protein contents. The results suggested using both antioxidants glutathione and ascorbic acid especially at 100 ppm of glutathione to improve wheat growth and yield.
Mohamed El-Sayed El-Awadi,
Safaa Reda El-Lethy,
Kawther Gad El-Rokiek,
Effect of the Two Antioxidants; Glutathione and Ascorbic Acid on Vegetative Growth, Yield and Some Biochemical Changes in Two Wheat Cultivars, Journal of Plant Sciences.
Vol. 2, No. 5,
2014, pp. 215-221.
Bolkhina, O., Virolainen, E., Fegerstedt K.V. 2003. Antioxidant, oxidative damage and oxygen deprivations stress. A Review Ann. Bot. 91,179-194.
Kumar V., Lemos M., Sharma, M., Shriram, V. 2013. Antioxidant and DNA damage protecting activities of Eulophia nuda Lindl. Free Radicals and Antioxidants. 3(2), 55-60.
El-Kobisy, D.S., Kady, K.A., Medani, R.A., Agamy, R.A. 2005. Response of pea plant (Pisum sativum L.) to treatment with ascorbic acid. Egypt. J. Appl. Sci. 20, 36-50.
Negm, A.Y. 1998. Foliar application of ascorbic acid and copper to wheat plants grown on sandy soils. Egypt. J. Appl. Sci. 13(5), 312-319.
Amin, A.A., Rashad, El-Sh.M. Gharib, F.A.E. 2008. Changes in morphological, physiological and reproductive characters of wheat plants as affected by foliar application with salicylic acid and ascorbic acid. Austr. J. Basic and Appli. Sci. 2(2), 252-261.
Bakry, A.B., Abdelraouf, R.E., Ahmed, M.A., El Karamany, M.F. 2012. Effect of drought stress and ascorbic acid foliar application on productivity and irrigation water use efficiency of wheat under newly reclaimed sandy soil. J. Appl. Sci. Res. 8(8), 4552-4558.
Gamal El-Din, K.M, Reda, F. 2003. Effect of presowing heat hardening and ascorbic acid treatments on growth, yield and some biochemical constituents of wheat (Triticum aectivum L.). Egypt. J. Appl. Sci. 18(12), 91-105.
Amin, B., Mahleghah, G., Mahmood, H.M.R., Hossein, M. 2009. Evaluation of interaction effect of drought stress with ascorbate and salicylic acid on some physiological and biochemical parameters in Okra (Hibiscus esculentus L.). Res. J. Biol. Sci. 4: 380–387.
Pompella, A., Visvikis, A., Paolicchi, A., Tata, V., Casini, A.F. 2003. The changing faces of glutathione, a cellular protagonist. Bioch. Pharm. 66 (8), 1499–503.
Asada, K., Takahashi, M. 1987. Production and scavenging of active oxygen in photosynthesis. In Photoinhibition. Edited by Kyle, D.J., Osmond, C.B. and Arntzen, C.J. Elsevier, Amsterdam, pp. 227–287. Noctor, G. Foyer, C.H. 1998. Ascorbate and glutathione: keeping active oxygen under control. Annu. Rev. Plant Physiol. Plant Mol. Biol. 49, 249–279.
Noctor, G. Foyer, C.H. 1998. Ascorbate and glutathione: keeping active oxygen under control. Annu. Rev. Plant Physiol. Plant Mol. Biol. 49, 249–279.
Henmi, K., Tsuboi, S., Demura, T., Fukuda, H., Iwabuchi, M., Ogawa, K. 2001. A possible role of glutathione and glutathione disulfide in tracheary element differentiation in the cultured mesophyll cells of Zinnia elegans. Plant Cell Physiol. 42, 673–676.
Ogawa, K., Hatano-Iwasaki, A., Yanagida, M. Henmi, K. 2002. The redox-dependent regulation of bolting and flowering in rosette-type plants. Flowering Newslett. 34, 45–51.
Ogawa, K., Hatano-Iwasaki, A., Yanagida, M. Iwabuchi, M. 2004. Level of glutathione is regulated by ATP-dependent ligation of glutamate and cysteine through photosynthesis in Arabidopsis thaliana: mechanism of strong interaction of light intensity with flowering. Plant Cell Physiol. 45, 1–8.
Wettstein, D. 1957. Chlorophyll lethal und der submikroskopiche Formwechsel der plastiden. Cell. Res. 12 (3), 427-433.
A. O.A.C. 2000. Official Methods of Analysis of Association of official chemists. Chlorophyll in plants, spectrophotometric method.17thed. Helrich K. (Ed) Inc: Arlingten, Virginia, USA., pp 28.
A.O.A.C. 1990. Official Methods of Analysis of Association Agriculture Chemists. 15ed. Washington, USA.
Plummer, D. (1978). An introduction to practical biochemistry. 2nd Ed. McGraw-Hall Mook Company Limited, London, UK., 144.
Snell, F.D., Snell, C.T. 1953. Colorimetric Methods. Volume III. Organic, D. Van Nostrand Company, Inc. Toronto, New York, London, 606 pp.
Dubois, M., Guilles, K.A, Hamilton, J.K., Rebers, P.A., Smith, F. 1956. Colorimetric method for determination of sugars and related substances. Anal. Chem. 28, 350-356.
Snedecor, G.W., Cochran, W.G. 1980. Statistical Methods. 7th ed. The Iowa State Univ. Press, Ames, Iowa, I A., 507 pp.
Parisy, V., Lucas, O., Antony, B., Jane, G., Felix, M. 2006. Identification of PAD2 as a γ-glutamylcysteine synthetase highlights the importance of glutathione in disease resistance of Arabidopsis. The Plant J. 49 (1), 159–72.
Rouhier, N., Lemaire, S.D., Jacquot, J.P. 2008. The role of glutathione in photosynthetic organisms: Emerging functions for glutaredoxins and glutathionylation. Annu. Rev. of Plant Biol. 59, 143–66.
Smirnoff, N. 1996. The function and metabolism of ascorbic acid in plants. Ann. Bot.78, 661- 669.
Franceschi, V.R., Tarlyn, N.M. 2002. L-ascorbic acid is accumulated in source leaf phloem and transported to sink tissues in plants. Plant Physiol. 130 (2), 649-656.
Talaat, I.M., Aziz, E.E. 2005. Stimulatory effects of glutathione, nicotinic acid and ascorbic acid on matricaria. Egypt. J. applied Sci. 20, 218-231.
Sanchez-Fernández, R., Fricker, M., Corben, L.B., White, N.S., Sheard, N., Leaver, C., Montagu, M.V., Inzé, D., May, M.J. 1997. Cell proliferation and hair tip growth in the Arabidopsis root are under mechanistically different forms of redox control. Proc. Natl Acad. Sci. USA. 94, 2745–2750.
Noctor, G., Mhamdi, A., Chaouch, S., Han, Y., Neukermans, J., Marquez-Garcia, B., Queval, G., Foyer, C.H 2012. Glutathione in plants: an integrated overview. Plant Cell Environ. 35(2):454-484
EL-Hosary, A.A., Hammam, G.Y., EL-Morsi, A., Hassan, E.A., EL-Awadi, M.E., Abdel-Baky Y.R. 2013. Effect of some Bio-regulators on growth and yield of some wheat varieties under newly cultivated land conditions. New York Sci.J. 6(6), 1-11.
Mohamed, N.E.M. 2013. Behaviour of wheat Cv. Masr-1 plants to foliar application of some vitamins. Nat. and Sci. 11(6), 1-5.
Srivalli, S., Khanna-Chopra, R. 2008. Delayed wheat flag leaf senescence due to the removal of spikelets is associated with increased activities of leaf antioxidant enzymes, reduced glutathione/oxidized glutathione ratio and oxidative damage to mitochondrial proteins. Plant Physiol. Biochem. 47, 663-670.
Coruzzi, G., Last, R., 2000. Amino acids. In: Biochemistry and molecular biology of plants. B. Buchanan, Gruissem, R. Jones (eds.). American Society of Plant Physiologists (pubs.). Maryland, USA. pp: 358-410
El-Rokiek, K.G, El-Awady, M.E., Abd El-Wahed, M.S.A. 2012. Physiological responses of wheat plants and accompanied weeds to derby herbicide and β-sitosterol bioregulator. J. Appl. Sci. Res., 8(4), 1918-1926.
Al-Muwayh, M.A. 2012. Effect of ascorbic acid on lipid and protein contents of Phaseouls vulgaris L grown at gaseous polluted areas in Riyadh city, Saudi Arabia. New Horizons in Sci. & Technol. (NHS&T). 1(3), 65-70