Biology and Infection Mechanisms of Cryptosporiopsis spp Fungus Causing Blight Disease on Cashew (Anacardium Occidentale L.)
Journal of Plant Sciences
Volume 2, Issue 6, December 2014, Pages: 266-275
Received: Oct. 23, 2014;
Accepted: Nov. 7, 2014;
Published: Nov. 23, 2014
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Menge Dominic, Biological Sciences Department, Maasai Mara University; P. O. Box 861 Narok, Kenya
Martha Makobe, Department of Botany, Jomo Kenyatta University of Agriculture and Technology, Nairobi, Kenya
Bonaventure Agboton, International Centre of Insect Physiology and Ecology (ICIPE); P. O. Box 30772-00100 Nairobi, Kenya
Shamte Shomari, Cashew Research Programme, Naliendele Agricultural Research Institute (NARI), P.O. Box 509, Mtwara, Tanzania
Andreas Tiedemann, University of Göttingen, Grisebachstrasse 6, 37077 Göttingen, Germany
Efficient isolation, purification and identification techniques of a new pathogenic fungus of cashew were developed. In the study, aspects related to identification, characterization of conidial morphology and infection of the Cryptosporiopsis spp fungus were investigated. Macroscopic observations of symptoms and signs of cashew blight disease as well as isolation, culturing and morphological characterization of its causative agent were carried out. Cryptosporiopsis spp fungus penetration and infection mechanisms on susceptible cashew leaves were studied. Cashew leaves were inoculated with fungal suspensions by pricking with pin, clipping with scissors and painting a fungal suspension onto the leaves with a paintbrush. Pathogenecity assays were performed on cashew leaves, nuts and apples. Irregular spot lesions, with a brown margin, formed on leaves especially on the young leaves. Direct conidial transfer was found to be the efficient method of isolation because it was less prone to contamination. Blight macroconidia are typical of Cryptosporiopsis having ellipsoidal, rounded at the apex, tapering into a scar at the base. Stomatal penetration of Cryptosporiopsis spp was visible through the simple staining technique using lactophenol solution. Penetration was by germinating conidia through a cuticle that caused an infection in leaves. Paint brush method of inoculation was found to be more effective as compared to other two methods based on the lesion size. Leaves inoculated with Cryptosporiopsis spp isolate began curling after 72 h thereafter developed dark, irregularly shaped spots with brown margins. An increased understanding of cashew blight is important for developing strategies for its management.
Biology and Infection Mechanisms of Cryptosporiopsis spp Fungus Causing Blight Disease on Cashew (Anacardium Occidentale L.), Journal of Plant Sciences.
Vol. 2, No. 6,
2014, pp. 266-275.
Adeniyi, D.O., Orisajo, S.B. Fademi, O.A. Adenuga, O.O. and Dongo, L.N. 2011. Physiological studies of fungi complexes associated with cashew diseases. J. Agric. Biol. Sci., 6: 34-38
Akaza M. J., Goran J. A. K. N`, `Guetta S-P.A. N, Kebe B. I., Tahi G.M. and Sangare A., (2009). Resistance to Phytophthora palmivora (Butler) Butler Assessed on Leaf Discs of Cacao (Theobroma cacao L.) Hybrid Trees. Asian Journal of Plant Pathology, 3: 106-118.
Akhtar, M. A. Abdul R. and Abdul H., 2008. Comparison of Methods of Inoculation of Xanthomonas Oryzae Pv.Oryzae in Rice Cultivars. Pak. J. Bot., 40(5): 2171-2175.
Akinwale, T. O. 2000. Cashew apple juice: Its use in fortifying the nutritional quality of some tropical fruits. Eur. Food Res. Technol. 211: 205-207.
Ammar M. I. and El-Naggar, M. A. (2011). Date Palm (Phoenix dactylifera L.) Fungal Diseases in Najran, Saudi Arabia. International Journal of Plant Pathology, 2: 126-135.
Azam-Ali, S. and Judge, E. 2000. The global cashew industry-opportunities for small-scale processors. In: Proceedings of the international workshop on cashew production and processing-Cashing in on Cashew pp. 1-10. Marawila: Sri Lanka.
Cavalcante, A. A. M., Rubensam, G. Picada, J.N. Da Silva, E.G. Moreira, J.C.F. and Henriques, J.A.P. 2003. Mutagenic evaluation, antioxidant potential and antimutagenic activity against hydrogen peroxide of cashew (Anacardium occidentale L.) apple juice and cajuina. Environ. Mol. Mut., 41: 360-369.
Global Plant Clinic 2010. “Plant Doctors” a Global Prescription for Plant Pests. Volume 90. www.cabi.org/Uploads/File/.../Sarah/Issues%20-%20biosecurity.pdf.
Hosen, M. I., Ahmed, A. U. and Islam, M. R. 2010. Variability and Pathogenicity in Bangladeshi Isolates of Botrytis cinerea Causing Botrytis Gray Mold in Chickpea (Cicer arietinum L.). Plant Pathology Journal, 9: 129-134.
Hubballi, M., Nakkeeran, S. Raguchander, T. Anand T. and Samiyappan, R. 2010. Effect of Environmental Conditions on Growth of Alternaria alternata Causing Leaf Blight of Noni. World Journal of Agricultural Sciences 6 (2): 171-177.
Hussain, A., Iqbal, S.M. Ayub N. and Haqqani,. A.M. 2003. Physiological Study of Sclerotium rolfsii Sacc.. Plant Pathology Journal, 2: 102-106.
Jabeen, R., S. Ur-Rahman and A. Rais,. 2011. Evaluating BLB Resistance / Aggressivness in Rice through Best Inoculum Concentration, Inoculation and Application Methods. Pak. J. Bot., 43(5): 2635-2638.
Kannan, V. R., Sumathi, C. S., Balasubramanian V. and Ramesh,. N. 2009. Elementary Chemical Profiling and Antifungal Properties of Cashew (Anacardium occidentale L.) Nuts. Botany Research International 2 (4): 253-257.
Latiffah Z., Hayati, M.Z. Baharuddin S. and Maziah Z., 2009. Identification and Pathogenicity of Fusarium Species Associated with Root Rot and Stem Rot of Dendrobium. Asian Journal of Plant Pathology, 3: 14-21.
Lopez, A. M. Q. and Lucas, J.A. 2010a. Reaction of dwarf cashew clones to Colletotrichum gloeosporioides isolates in controlled environment. Sci. Agric. (Piracicaba, Braz.), v.67, n.2, p.228-235.
Lopez, A. M. Q. and. Lucas,. J. A. 2010b. Reaction of dwarf cashew clones to Colletotrichum gloeosporioides isolates in controlled environment. Sci Agric. (Piracicaba, Braz.), V.67,n.2.
Marc, A., Achille, T. F. Mory, G. Koffi, P.V.N. Georges,. A.N. 2011. Minerals composition of the cashew apple juice (Anacardium occidentale L.) of Yamoussoukro, Cote D’ivoire. Pakistan Journal of Nutrition 10(12): 1109-1114.
Mehta, C. R. and Patel, N. R. 2001. StatXact-5 for windows. Manual, Cytel Software Cooperation, Cambridge, USA.
Ogunwolu, S. O., Henshaw, F. O. Mock H.P. and Matro., A. 2010. Production of protein concentrate and isolate from cashew (Anacardium occidentale L.) nut. African Journal of Food Agriculture Nutrition and Development.
Olmstead, J.W. and G.A. Lang, 2000. A Leaf Disk Assay for Screening Sweet Cherry Genotypes for Susceptibility to Powdery Mildew. Hortscience 35(2):274–277.
Samiyeh R, Adam P. and Sijam, K. 2011. First Report of Diaporthe/Phomopsis Complex Isolates in Soybean from Malaysia and Their Longevity in Stored Seeds. Asian Journal of Plant Pathology, 5: 146-154.
SAS Institute Inc 2003. SAS/STAT User's Guide. Version 9.1, SAS Institute Inc. Cary, North Carolina, USA.
Sharma, P., Singh N. and Verma, O.P. (2011). First Report of Curvularia lunata Associated with Leaf Spot of Amaranthus spinosus. Asian Journal of Plant Pathology, 5: 100-101.
Sijaona, M. E. R., Reeder, R. H. and Waller, J. M. 2006. Cashew leaf and nut blight – A new disease of cashew in Tanzania caused by Cryptosporiopsis sp. Plant Pathology 55 576.
Umeh, J. C., 2007. Economic potentials of cashew. Proceedings of the Repositioning Cashew for Economic Growth and Development, July 10-11, 2007, Confluence Beach Hotel, Lokoja, Kogi State, Nigeria.
Venisse, J. S., Malnoy, M. Faize, M. Paulin, J. P. and Brisset, M. N. 2002. Modulation of defense responses of Malus spp. during compatible and incompatible interactions with Erwinia amylovora. Mol Plant Microbe Interact 15:1204-1212.
Verkley, G. J., Zijlstra, J. D. Summerbell, R. C. and Berendse, F. 2003. Phylogeny and taxonomy of root-inhabiting Cryptosporiopsis species and C. rhizophila sp. nov., a fungus inhabiting roots of several Ericaceae. Mycol. Res.,107: 689–698.
Webster, J. and Weber, R. 2007. Introduction to Fungi. 3rd Edn., Cambridge University Press, Cambridge, UK., Pages: 841.
Yousefi, A. and Shahri, M. H. 2009. Brown Spot Disease of Peach and Apricot Trees, Pathogenicity and Overwinter. Asian Journal of Plant Pathology, 3: 61-69.