Journal of Plant Sciences
Volume 2, Issue 6, December 2014, Pages: 299-303
Received: Nov. 20, 2014;
Accepted: Dec. 11, 2014;
Published: Dec. 17, 2014
Views 2909 Downloads 165
Dixa Singh, Department of Chemistry, H. N. B. Garhwal University Campus- Pauri (Uttarakhand), India
V. S. Singh, Department of Chemistry, H. N. B. Garhwal University Campus- Pauri (Uttarakhand), India
Anti- oxidants are crucial to human health. They have capacity to neutralize the free radicals responsible for giving birth to a variety of diseases. A diet rich in foods with high levels of anti-oxidants is associated with longevity and good health. We tried to replace the chemical anti-oxidants by herbal anti-oxidants. In plants, the anti-oxidants are present as flavonoids,so the flavonoids in Urena lobata were isolated , identified, quantitatively estimated and tested for their anti-oxidant activity.The flavonoids were extracted from the stem and the leaves separately to know the relative contents. Quercetin and Kaempferol were found to exist in the plant material.These were qualitatively tested by the method of Thin Layer Chromatography (TLC). The isolation, purification and quantitative content determination was performed by preparative thin layer chromatography (PTLC). The anti-oxidant activity of the methanolic plant extract was examined by DPPH radical scavenging activity method. The anti-oxidant ability of the plant extract was found comparable with Vitamin C.
V. S. Singh,
Urena lobata: A Green Source of Anti-Oxidant, Journal of Plant Sciences.
Vol. 2, No. 6,
2014, pp. 299-303.
V. Lovo, A. Patil, A. Pathak, N. Chandra, Free radicals, Antioxidants and Functional Foods : Impact on Human Health, Pharmacognosy Reviews, 4(8),(2010), 118-126.
A.B., Shinde, N.B Shrigadi, S.D. Samant, Tert-Butylation of Phenols using Tert-Butyl Alcohol in the Presence of FeCl3- modified Montmorillonite K10 Appl. Catal. A: Gen., 276, (2004), 5-8.
N. Bhatt, P. Sharma, A. Patel, A. Selvam , Supported 12 – Tungstophosphoricacid : An Efficient And Selective Solid Acid –Catalyst for Tert- Butylation of Phenol and Cresols, Catal. Comm., 9(7), (2008) , 1545 -1550.
H.U. Shen, Z.M.A. Judeh, C.B. Ching, Q.H. Xia,. Comparative Studies on Alkylation of phenol With Tert- Butyl Alcohol in Presence of Liquid or Solid Acid Catalysts in Ionic Liquids, J.Mol. Catal. A, .212 (1-2), (2004), 301 -308.
A. R. Proteggente, A.S. Pannala, L. Van Buren, E.Wagner, S. Wiseman, F.Vande Put, C.Dacombe, C.A. Rice- Evans, The Antioxidant Activity of Regularly Consumed Fruit and Vegetables Reflects their Phenolic and Vitamin C Composition, Free Radical Research, 36 (2), (2002), 217-233.
J.K. Francis, Atlas of Florida Vascular Plants , Institute of Systematic Botany, University of South Florida, Tampa, FL., 2003.
R. Freedman, Famine Foods: Malvceae , Perdue University , Perdue , IN , 1998.
Food and Agriculture Organization of the United Nations, Rome, in its report Food Composition Table for Use in Africa, 2003.
S.S.Subramanian, S. Nagarajan , Flavonoids of the seeds of Crotlaria retusa and C. striata, Curr. Sci., 38 , (1969) , 65 – 68.
T.J.Mabry, K.R. Markham, M.B. Thomas, The systematic identification of flavonoids, Springer- Verlag, Berlin, 1970.
V. Bondet, W. Brand-Williams, C. Berset, Kinetics and Mechanisms of Anti-oxidant activity using the DPPH Free Radical Method, Food Science and Technology, 30, (1997), 609-615.
W. Brand –Williums , M.E. Cuvelier , C. Berset , Use of a Free radical Method to evaluate Anti-oxidant Activity , Food Science And Technology, 28, (1995), 25-30.