Urena lobata: A Green Source of Anti-Oxidant
Journal of Plant Sciences
Volume 2, Issue 6, December 2014, Pages: 299-303
Received: Nov. 20, 2014; Accepted: Dec. 11, 2014; Published: Dec. 17, 2014
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Authors
Dixa Singh, Department of Chemistry, H. N. B. Garhwal University Campus- Pauri (Uttarakhand), India
V. S. Singh, Department of Chemistry, H. N. B. Garhwal University Campus- Pauri (Uttarakhand), India
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Abstract
Anti- oxidants are crucial to human health. They have capacity to neutralize the free radicals responsible for giving birth to a variety of diseases. A diet rich in foods with high levels of anti-oxidants is associated with longevity and good health. We tried to replace the chemical anti-oxidants by herbal anti-oxidants. In plants, the anti-oxidants are present as flavonoids,so the flavonoids in Urena lobata were isolated , identified, quantitatively estimated and tested for their anti-oxidant activity.The flavonoids were extracted from the stem and the leaves separately to know the relative contents. Quercetin and Kaempferol were found to exist in the plant material.These were qualitatively tested by the method of Thin Layer Chromatography (TLC). The isolation, purification and quantitative content determination was performed by preparative thin layer chromatography (PTLC). The anti-oxidant activity of the methanolic plant extract was examined by DPPH radical scavenging activity method. The anti-oxidant ability of the plant extract was found comparable with Vitamin C.
Keywords
Urena Lobata, Quercetin, Kaempferol, Myrecetin, Silica Gel-G, Ascorbic Acid and DPPH
To cite this article
Dixa Singh, V. S. Singh, Urena lobata: A Green Source of Anti-Oxidant, Journal of Plant Sciences. Vol. 2, No. 6, 2014, pp. 299-303. doi: 10.11648/j.jps.20140206.16
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