Effect of Pb-Stress on Growth and Mineral Status of Two Groundnut (Arachis hypogaea L.) Cultivars
Journal of Plant Sciences
Volume 2, Issue 6, December 2014, Pages: 304-310
Received: Nov. 26, 2014;
Accepted: Dec. 9, 2014;
Published: Dec. 19, 2014
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Ambekar Nareshkumar , Plant Molecular Biology Laboratory, Department of Botany, Sri Krishnadevaraya University, Anantapuramu-515003, Andhra Pradesh, India
B. V. Krishnappa , Plant Molecular Biology Laboratory, Department of Botany, Sri Krishnadevaraya University, Anantapuramu-515003, Andhra Pradesh, India
T. V. Kirankumar , Plant Molecular Biology Laboratory, Department of Botany, Sri Krishnadevaraya University, Anantapuramu-515003, Andhra Pradesh, India
K. Kiranmai , Plant Molecular Biology Laboratory, Department of Botany, Sri Krishnadevaraya University, Anantapuramu-515003, Andhra Pradesh, India
U. Lokesh , Plant Molecular Biology Laboratory, Department of Botany, Sri Krishnadevaraya University, Anantapuramu-515003, Andhra Pradesh, India
O. Sudhakarbabu , Plant Molecular Biology Laboratory, Department of Botany, Sri Krishnadevaraya University, Anantapuramu-515003, Andhra Pradesh, India
Chinta Sudhakar , Plant Molecular Biology Laboratory, Department of Botany, Sri Krishnadevaraya University, Anantapuramu-515003, Andhra Pradesh, India
Heavy metal pollution of air and agricultural soils is one of the most important ecological problems on world scale. Among the heavy metals, lead (Pb) is one of the common environmental pollutants. To investigate Pb effects on nutrient uptake, two groundnut (Arachis hypogaea L.) cultivars (cultivar K6 and cultivar K9) were grown in pot cultures and stressed with lead nitrate (Pb(NO3)2) at four concentrations (100, 200, 400 and 800 ppm). Pb is accumulated in roots and leaf tissues in dose dependent manner in both groundnut cultivars, which resulted in reduced root and shoot growth and lower uptake of all mineral ions tested. The content of mineral ions such as Ca, Na, Mg, Co, Cu, Ni, Zn and Mn reduced in root and leaf tissues of both cultivars due to Pb stress. But the reduction in mineral ion content was less in cultivar K6 than in cultivar K9. The deficiency of mineral nutrients correlates in a strong decrease in the contents of total chlorophyll, and anthocyanin in both cultivars, but these effects are less pronounced in cultivar K6 than in cultivar K9.
Ambekar Nareshkumar ,
B. V. Krishnappa ,
T. V. Kirankumar ,
K. Kiranmai ,
U. Lokesh ,
O. Sudhakarbabu ,
Chinta Sudhakar ,
Effect of Pb-Stress on Growth and Mineral Status of Two Groundnut (Arachis hypogaea L.) Cultivars, Journal of Plant Sciences.
Vol. 2, No. 6,
2014, pp. 304-310.
A. M. Reddy, S. G. Kumar, G. Jyothsnakumar, S. Thimmanaik, and C. Sudhakar, Lead induced changes in antioxidant metabolism of horsegram (Macrotyloma uniflorum (Lam.) Verdc.) and bengalgram (Cicer arietinum L.). Chemosphere, 60 (2005) 97-104.
M. Raghunath, E. A. Putnam, T. Ritty, D. Hamstra, E. S. Park, M. Tschödrich-Rotter, R. Peters, A. Rehemtulla, and D. M. Milewicz, Carboxy-terminal conversion of profibrillin to fibrillin at a basic site by PACE/furin-like activity required for incorporation in the matrix. Journal of Cell Science, 112 (1999) 1093-1100.
S. Gallego, M. Benavides, and M. Tomaro, Involvement of an antioxidant defense system in the adaptive response to heavy metal ions in Helianthus annuus L. cells. Plant Growth Regulation, 36 (2002) 267-273.
F. V. Assche, and H. Clijsters, Effects of metal on enzyme activity in plants. Plant Cell & Environment, 13 (1990) 195-206.
C. M. Luna, C. A. Gonzalez, and V. S. Trippi, Oxidative damage caused by an excess of copper in oat leaves. Plant & Cell Physiology, 35 (1994) 11-15.
A. Fargasova, Phytotoxic effects of Cd, Zn, Pb, Cu and Fe on Sinapis alba L. seedling and their accumulation in roots and shoots. Biologia Plantarum, 44 (2001) 471-473.
M. Moustakas, T. Lanaras, L. Symeonidis, and S. Karataglis. Growth and some photosynthetic characteristics of field grown Avena sativa under copper and lead stress. Photosynthetica, 30 (1994) 389-396.
P. Sharma, and R. S. Dubey, Ascorbate peroxidase from rice seedlings; properties of enzyme isoforms, effects of stress and protective roles of osmolytes. Plant Science, 167 (2004) 541-550.
A. Kabata-Pendias, and H. Pendias, Trace elements in soils and plants. 1992, 2nd edn. CRC Press, Boca Raton, London.
J. Varma, and N. K. Dubey, Efficacy of essential oils of Caesulia axillaris and Mentha arvensis against some storage pests causing biodeterioration of food commodities. International Journal of Food Microbiology, 68 (2001) 207-210.
J. A. C. Verkleij, A. Golan-Goldhirsh, D. M. Antosiewisz, J. P. Schwitzguébel, and P. Schröder, Dualities in plant tolerance to pollutants and their uptake and translocation to the upper plant parts. Environmental and Experimental Botany, 1 (2009) 10-22.
H. H. Zahran, Rhizobium-Legume symbiosis and nitrogen fixation under severe conditions and in an arid climate. In Microbiology and Molecular Biology Reviews, American society for microbiology, (1999) 63, pp. 968-989.
J. D. Hiscox, and G. F. Israelstam, A method for extraction of chlorophyll from leaf tissues without maceration. Canadian Journal of Botany, 57 (1979) 1332-1334.
D. I. Arnon, Copper enzymes in isolated chloroplasts. Polyphenol oxidase in Beta vulgaris. Plant Physiology, 24 (1949) 1-15.
H. Lange, W. Shropshire, and H. Mohr, An Analysis of Phytochrome-mediated Anthocyanin Synthesis. Plant Physiology, 47 (1971) 649-655.
I. E. Akinci, S. Akinci, and K. Yilmaz, Response of tomato (Solanum lycopersicum L.) to lead toxicity: growth, element uptake, chlorophyll and water content. African Journal of Agriculture Research 5 (2010) 416-423.
C. Wang, Y. Tian, X. Wang, J. Geng, J. Jiang, H. Yu, and C. Wang, Lead-contaminated soil induced oxidative stress, defence response and its indicative biomarkers in roots of Vicia faba seedlings. Ecotoxicology, 19 (2010) 1130-1139.
Y. Ekmekçi, D. Tanyolaç, and B. Ayhan, A crop tolerating oxidative stress induced by excess lead: maize. Acta Physiologiae Plantarum, 31 (2009) 319-330.
C. Sudhakar, L. Syamalabai, and K. Veeranjaneyulu, Lead tolerance of certain legume species grown on lead ore tailings. Agric Ecosyst Environ, 41 (1992) 253-261.
R. Alkhatib, J. Maruthavanan, S. Ghoshroy, R. Steiner, T. Sterling, and R. Creamer, Physiological and ultra structural effects of lead on tobacco. Biologia Plantarum, 56 (2011) 711-716.
Y. Yang, X. Wei, J. Lu, J. You, W. Wang, and R. Shi, Lead-induced phytotoxicity mechanism involved in seed germination and seedling growth of wheat (Triticum aestivum L.). Ecotoxicology and Environmental Safety, 73 (2010) 1982-1987.
S. D. Lane, and E. S. Martin, A histochemical investigation of lead uptake in Raphanus sativus. New Phytology, 79 (1977) 281-286.
R. Przymusinski, M. Spychala, and E. A. Gwozdz, Inorganic lead changes growth polypeptide pattern of lupin roots. Biochemie und Physiolgie Pflanzen, 187 (1991) 51-57.
R. M. Cox, and T. C. Hutchinson, Multiple and co-tolerance in the grass Deschampsia cespitosa: adaptation, pre adaptation and cost. Journal of Plant Nutrition, 3 (1981) 731-741.
S. A. Bharwana, S. Ali, M. A. Farooq, N. Iqbal, F. Abbas, and M. S. A. Ahmad, Alleviation of lead toxicity by silicon is related to elevated photosynthesis, antioxidant enzymes suppressed lead uptake and oxidative stress in cotton. Journal of Bioremediation and Biodegradation, 4 (2013) 1-11.
H. N. Azad, A. H. Shiva, and R. Malekpour, Toxic effects of lead on growth and some biochemical and ionic parameters of sunflower (Helianthus annuus L) seedlings. Current Research Journal Biological Sciences, 3 (2011) 398-403.
S. S. Gill, N. A. Khan, and N. Tuteja, Cadmium at high dose perturbs growth, photosynthesis and nitrogen metabolism while at low dose it up regulates sulfur assimilation and antioxidant machinery in garden cress (Lepidium sativum L.). Plant Science, 182 (2012) 112-120.
S. Zhao, X. Ye, and J. Zheng, Lead-induced changes in plant morphology, cell ultrastructure, growth and yields of tomato. African Journal of Biotechnology, 10 (2011) 10116-10124.
A. J. M. Baker, and P. L. Walker, Ecophysiology of metal uptake by tolerant plants. In: A. J. Shaw (Ed.), Heavy metal tolerance in plants: Evolutionary aspects. CRC Press, Boca Raton, FL, pp. 155-177.
T. C. Broyer, C. M. Johnson, R. E. Paul, Some aspects of lead in plant nutrition. Plant and Soil, 36 (1972) 301-313.
J. Liu, K. Li, J. Xu, Z. Zhang, T. Ma, J. Xang, Q. Zhu, Lead toxicity, uptake and translocation in different rice cultivars. Plant Science, 165 (2004) 793-802.
D. Cargnelutti, L. A. Tabaldi, R. M. Spanevello, G. O. Jucoski, V. Battisti, M. Redin, C. E. Linares, V. L. Dressler, E. M. M. Flores, F. T. Nicoloso, V. M. Morsch, and M. R. Schetinger, Mercury toxicity induces oxidative stress in growing cucumber seedlings. Chemosphere, 65 (2006) 999-1006.
H. S. El-Beltagi, and A. A. Mohamed, Changes in non protein thiols, some antioxidant enzymes activity and ultra structural alteration in radish plant (Raphanus sativus L.) grown under lead toxicity. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 38 (2010) 76-85.
L. Chen, S. Gao, P. Zhu, Y, Liu, T. Hu, and J. Zhang, Comparative study of metal resistance and accumulation of lead and zinc in two poplars. Physiologia Plantarum, 151 (2014) 390-405.
P. Wang, S. Zhang, C. Wang, and J. Lu, Effects of Pb on the oxidative stress and antioxidant response in a Pb bioaccumulator plant Vallisneria natans. Ecotoxicology and Environmental Safety, 78 (2012) 28-34.
A. Kumar, M. N. V. Prasad, and O. Sytar, Lead toxicity, defense strategies and associated indicative biomarkers in Talinum triangulare grown hydroponically. Chemosphere, 89 (2012) 1056-1065.