Rheum species are medicinally important plants due to the presence of anthracene derivatives. This study was designed to determine the antioxidative, cytotoxic and apoptotic properties of Rheum ribes shoot and root ethyl acetate extracts using human promyelocytic leukemia (HL-60) cell line as a model system. R. ribes shoot and root dry powder samples were prepared and extracted with ethyl acetate. The extracts were revealed to be a potential scavenger of DPPH radicals (IC50 value of 206.28 μg/ml for shoot and 10.92 μg/ml for root) and the chemical composition of the extracts was quantified by colorimetric determination of total phenol (GAE) and flavonoid (CAE) contents. HL–60 cells were cultured in the presence of various concentrations of extracts up to 72 h. R. ribes inhibited the survival of HL-60 cells in a concentration- and time-dependent manner, shown by XTT assay. R. ribes caused HL-60 cells apoptosis via formation of phosphatidylserine externalization, as evidenced by flow cytometry. Exposure of HL-60 cells to higher concentrations of extracts for 72 h resulted in a shift of 87% of the cell population from normal to the early/late apoptotic stage. These findings suggest that Rheum ribes ethyl acetate root extracts exhibits potential antioxidant and cytotoxic properties against HL-60 cells better than shoot extracts and exert their toxicity via induction of apoptosis.
| Published in | Journal of Plant Sciences (Volume 2, Issue 6) |
| DOI | 10.11648/j.jps.20140206.22 |
| Page(s) | 339-346 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
Rheum ribes, Antioxidant, HL-60, Cytotoxicity, Apoptosis
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APA Style
Pembegul Uyar, Nursen Coruh, Mesude İscan. (2015). Evaluation of in Vitro Antioxidative, Cytotoxic and Apoptotic Activities of Rheum ribes Ethyl Acetate Extracts. Journal of Plant Sciences, 2(6), 339-346. https://doi.org/10.11648/j.jps.20140206.22
ACS Style
Pembegul Uyar; Nursen Coruh; Mesude İscan. Evaluation of in Vitro Antioxidative, Cytotoxic and Apoptotic Activities of Rheum ribes Ethyl Acetate Extracts. J. Plant Sci. 2015, 2(6), 339-346. doi: 10.11648/j.jps.20140206.22
AMA Style
Pembegul Uyar, Nursen Coruh, Mesude İscan. Evaluation of in Vitro Antioxidative, Cytotoxic and Apoptotic Activities of Rheum ribes Ethyl Acetate Extracts. J Plant Sci. 2015;2(6):339-346. doi: 10.11648/j.jps.20140206.22
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Download@article{10.11648/j.jps.20140206.22,
author = {Pembegul Uyar and Nursen Coruh and Mesude İscan},
title = {Evaluation of in Vitro Antioxidative, Cytotoxic and Apoptotic Activities of Rheum ribes Ethyl Acetate Extracts},
journal = {Journal of Plant Sciences},
volume = {2},
number = {6},
pages = {339-346},
doi = {10.11648/j.jps.20140206.22},
url = {https://doi.org/10.11648/j.jps.20140206.22},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jps.20140206.22},
abstract = {Rheum species are medicinally important plants due to the presence of anthracene derivatives. This study was designed to determine the antioxidative, cytotoxic and apoptotic properties of Rheum ribes shoot and root ethyl acetate extracts using human promyelocytic leukemia (HL-60) cell line as a model system. R. ribes shoot and root dry powder samples were prepared and extracted with ethyl acetate. The extracts were revealed to be a potential scavenger of DPPH radicals (IC50 value of 206.28 μg/ml for shoot and 10.92 μg/ml for root) and the chemical composition of the extracts was quantified by colorimetric determination of total phenol (GAE) and flavonoid (CAE) contents. HL–60 cells were cultured in the presence of various concentrations of extracts up to 72 h. R. ribes inhibited the survival of HL-60 cells in a concentration- and time-dependent manner, shown by XTT assay. R. ribes caused HL-60 cells apoptosis via formation of phosphatidylserine externalization, as evidenced by flow cytometry. Exposure of HL-60 cells to higher concentrations of extracts for 72 h resulted in a shift of 87% of the cell population from normal to the early/late apoptotic stage. These findings suggest that Rheum ribes ethyl acetate root extracts exhibits potential antioxidant and cytotoxic properties against HL-60 cells better than shoot extracts and exert their toxicity via induction of apoptosis.},
year = {2015}
}
TY - JOUR T1 - Evaluation of in Vitro Antioxidative, Cytotoxic and Apoptotic Activities of Rheum ribes Ethyl Acetate Extracts AU - Pembegul Uyar AU - Nursen Coruh AU - Mesude İscan Y1 - 2015/01/28 PY - 2015 N1 - https://doi.org/10.11648/j.jps.20140206.22 DO - 10.11648/j.jps.20140206.22 T2 - Journal of Plant Sciences JF - Journal of Plant Sciences JO - Journal of Plant Sciences SP - 339 EP - 346 PB - Science Publishing Group SN - 2331-0731 UR - https://doi.org/10.11648/j.jps.20140206.22 AB - Rheum species are medicinally important plants due to the presence of anthracene derivatives. This study was designed to determine the antioxidative, cytotoxic and apoptotic properties of Rheum ribes shoot and root ethyl acetate extracts using human promyelocytic leukemia (HL-60) cell line as a model system. R. ribes shoot and root dry powder samples were prepared and extracted with ethyl acetate. The extracts were revealed to be a potential scavenger of DPPH radicals (IC50 value of 206.28 μg/ml for shoot and 10.92 μg/ml for root) and the chemical composition of the extracts was quantified by colorimetric determination of total phenol (GAE) and flavonoid (CAE) contents. HL–60 cells were cultured in the presence of various concentrations of extracts up to 72 h. R. ribes inhibited the survival of HL-60 cells in a concentration- and time-dependent manner, shown by XTT assay. R. ribes caused HL-60 cells apoptosis via formation of phosphatidylserine externalization, as evidenced by flow cytometry. Exposure of HL-60 cells to higher concentrations of extracts for 72 h resulted in a shift of 87% of the cell population from normal to the early/late apoptotic stage. These findings suggest that Rheum ribes ethyl acetate root extracts exhibits potential antioxidant and cytotoxic properties against HL-60 cells better than shoot extracts and exert their toxicity via induction of apoptosis. VL - 2 IS - 6 ER -
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