In Vitro Shoot and Callus Induction and Alkaloid Contents of Ephedra intermedia (Schrenket) of Iran

Azra Ataei Azimi; Babak Delnavaz Hashemloian

doi:doi:10.11648/j.jps.20150301.11

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In Vitro Shoot and Callus Induction and Alkaloid Contents of Ephedra intermedia (Schrenket) of Iran

Azra Ataei Azimi, Babak Delnavaz Hashemloian

Published in Journal of Plant Sciences (Volume 3, Issue 1)

Received: 12 December 2014 Accepted: 18 December 2014 Published: 19 January 2015

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Abstract

Ephedra intermedia(Schrenket) isan evergreen shrub belongs to family Ephedraceae.The present study deals with Invitro callus induction, multiple shoots regeneration and alkaloid production of E.intermedia. Callus andmultiple shoots formation were initiated on MS medium supplementedwith different concentrations of 1-Naphthalene Acetic Acid (NAA)and Kinetin (Kn)hormonescombination. Alkaloid analysis was done by spectrophotometry, TLC, HPLC methods.Alkaloid was found in all tissue cultures and E. intermedia organs. Total alkaloids were higher in roots and some shoots cultures than of ephedra shoots and seeds, cultures shoots and callus. The ephedrine and pseudoephedrine were higher in ephedra roots than in ephedra shoots and seeds, all callusculturesand some shoots cultures but they balancedin roots with shoots cultures of 15 mgl-1 NAA supplemented with 10 mgl-1 Kn.This study can be utilized to initiate multiple shoots culture of Ephedrathat may provide a good source of pharmacologically active constituents.

Published in	Journal of Plant Sciences (Volume 3, Issue 1)
DOI	10.11648/j.jps.20150301.11
Page(s)	1-8
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Ma-Haung, Multiple Shoots, Ephedrine, Pseudoephedrine

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Azra Ataei Azimi, Babak Delnavaz Hashemloian. (2015). In Vitro Shoot and Callus Induction and Alkaloid Contents of Ephedra intermedia (Schrenket) of Iran. Journal of Plant Sciences, 3(1), 1-8. https://doi.org/10.11648/j.jps.20150301.11

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ACS Style

Azra Ataei Azimi; Babak Delnavaz Hashemloian. In Vitro Shoot and Callus Induction and Alkaloid Contents of Ephedra intermedia (Schrenket) of Iran. J. Plant Sci. 2015, 3(1), 1-8. doi: 10.11648/j.jps.20150301.11

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AMA Style

Azra Ataei Azimi, Babak Delnavaz Hashemloian. In Vitro Shoot and Callus Induction and Alkaloid Contents of Ephedra intermedia (Schrenket) of Iran. J Plant Sci. 2015;3(1):1-8. doi: 10.11648/j.jps.20150301.11

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@article{10.11648/j.jps.20150301.11,
  author = {Azra Ataei Azimi and Babak Delnavaz Hashemloian},
  title = {In Vitro Shoot and Callus Induction and Alkaloid Contents of Ephedra intermedia (Schrenket) of Iran},
  journal = {Journal of Plant Sciences},
  volume = {3},
  number = {1},
  pages = {1-8},
  doi = {10.11648/j.jps.20150301.11},
  url = {https://doi.org/10.11648/j.jps.20150301.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jps.20150301.11},
  abstract = {Ephedra intermedia(Schrenket) isan evergreen shrub belongs to family Ephedraceae.The present study deals with Invitro callus induction, multiple shoots regeneration and alkaloid production of E.intermedia. Callus andmultiple shoots formation were initiated on MS medium supplementedwith different concentrations of 1-Naphthalene Acetic Acid (NAA)and Kinetin (Kn)hormonescombination. Alkaloid analysis was done by spectrophotometry, TLC, HPLC methods.Alkaloid was found in all tissue cultures and E. intermedia organs. Total alkaloids were higher in roots and some shoots cultures than of ephedra shoots and seeds, cultures shoots and callus. The ephedrine and pseudoephedrine were higher in ephedra roots than in ephedra shoots and seeds, all callusculturesand some shoots cultures but they balancedin roots with shoots cultures of 15 mgl-1 NAA supplemented with 10 mgl-1 Kn.This study can be utilized to initiate multiple shoots culture of Ephedrathat may provide a good source of pharmacologically active constituents.},
 year = {2015}
}

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TY  - JOUR
T1  - In Vitro Shoot and Callus Induction and Alkaloid Contents of Ephedra intermedia (Schrenket) of Iran
AU  - Azra Ataei Azimi
AU  - Babak Delnavaz Hashemloian
Y1  - 2015/01/19
PY  - 2015
N1  - https://doi.org/10.11648/j.jps.20150301.11
DO  - 10.11648/j.jps.20150301.11
T2  - Journal of Plant Sciences
JF  - Journal of Plant Sciences
JO  - Journal of Plant Sciences
SP  - 1
EP  - 8
PB  - Science Publishing Group
SN  - 2331-0731
UR  - https://doi.org/10.11648/j.jps.20150301.11
AB  - Ephedra intermedia(Schrenket) isan evergreen shrub belongs to family Ephedraceae.The present study deals with Invitro callus induction, multiple shoots regeneration and alkaloid production of E.intermedia. Callus andmultiple shoots formation were initiated on MS medium supplementedwith different concentrations of 1-Naphthalene Acetic Acid (NAA)and Kinetin (Kn)hormonescombination. Alkaloid analysis was done by spectrophotometry, TLC, HPLC methods.Alkaloid was found in all tissue cultures and E. intermedia organs. Total alkaloids were higher in roots and some shoots cultures than of ephedra shoots and seeds, cultures shoots and callus. The ephedrine and pseudoephedrine were higher in ephedra roots than in ephedra shoots and seeds, all callusculturesand some shoots cultures but they balancedin roots with shoots cultures of 15 mgl-1 NAA supplemented with 10 mgl-1 Kn.This study can be utilized to initiate multiple shoots culture of Ephedrathat may provide a good source of pharmacologically active constituents.
VL  - 3
IS  - 1
ER  -

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Author Information

Azra Ataei Azimi

Associate professorof plant cell and developmental biology, Department of Biology, Saveh Branch, Islamic Azad University, Saveh, Iran
Babak Delnavaz Hashemloian

Associate professorof plant cell and developmental biology, Department of Biology, Saveh Branch, Islamic Azad University, Saveh, Iran

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APA Style

Azra Ataei Azimi, Babak Delnavaz Hashemloian. (2015). In Vitro Shoot and Callus Induction and Alkaloid Contents of Ephedra intermedia (Schrenket) of Iran. Journal of Plant Sciences, 3(1), 1-8. https://doi.org/10.11648/j.jps.20150301.11

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ACS Style

Azra Ataei Azimi; Babak Delnavaz Hashemloian. In Vitro Shoot and Callus Induction and Alkaloid Contents of Ephedra intermedia (Schrenket) of Iran. J. Plant Sci. 2015, 3(1), 1-8. doi: 10.11648/j.jps.20150301.11

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AMA Style

Azra Ataei Azimi, Babak Delnavaz Hashemloian. In Vitro Shoot and Callus Induction and Alkaloid Contents of Ephedra intermedia (Schrenket) of Iran. J Plant Sci. 2015;3(1):1-8. doi: 10.11648/j.jps.20150301.11

Copy | Download

@article{10.11648/j.jps.20150301.11,
  author = {Azra Ataei Azimi and Babak Delnavaz Hashemloian},
  title = {In Vitro Shoot and Callus Induction and Alkaloid Contents of Ephedra intermedia (Schrenket) of Iran},
  journal = {Journal of Plant Sciences},
  volume = {3},
  number = {1},
  pages = {1-8},
  doi = {10.11648/j.jps.20150301.11},
  url = {https://doi.org/10.11648/j.jps.20150301.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jps.20150301.11},
  abstract = {Ephedra intermedia(Schrenket) isan evergreen shrub belongs to family Ephedraceae.The present study deals with Invitro callus induction, multiple shoots regeneration and alkaloid production of E.intermedia. Callus andmultiple shoots formation were initiated on MS medium supplementedwith different concentrations of 1-Naphthalene Acetic Acid (NAA)and Kinetin (Kn)hormonescombination. Alkaloid analysis was done by spectrophotometry, TLC, HPLC methods.Alkaloid was found in all tissue cultures and E. intermedia organs. Total alkaloids were higher in roots and some shoots cultures than of ephedra shoots and seeds, cultures shoots and callus. The ephedrine and pseudoephedrine were higher in ephedra roots than in ephedra shoots and seeds, all callusculturesand some shoots cultures but they balancedin roots with shoots cultures of 15 mgl-1 NAA supplemented with 10 mgl-1 Kn.This study can be utilized to initiate multiple shoots culture of Ephedrathat may provide a good source of pharmacologically active constituents.},
 year = {2015}
}

Copy | Download

TY  - JOUR
T1  - In Vitro Shoot and Callus Induction and Alkaloid Contents of Ephedra intermedia (Schrenket) of Iran
AU  - Azra Ataei Azimi
AU  - Babak Delnavaz Hashemloian
Y1  - 2015/01/19
PY  - 2015
N1  - https://doi.org/10.11648/j.jps.20150301.11
DO  - 10.11648/j.jps.20150301.11
T2  - Journal of Plant Sciences
JF  - Journal of Plant Sciences
JO  - Journal of Plant Sciences
SP  - 1
EP  - 8
PB  - Science Publishing Group
SN  - 2331-0731
UR  - https://doi.org/10.11648/j.jps.20150301.11
AB  - Ephedra intermedia(Schrenket) isan evergreen shrub belongs to family Ephedraceae.The present study deals with Invitro callus induction, multiple shoots regeneration and alkaloid production of E.intermedia. Callus andmultiple shoots formation were initiated on MS medium supplementedwith different concentrations of 1-Naphthalene Acetic Acid (NAA)and Kinetin (Kn)hormonescombination. Alkaloid analysis was done by spectrophotometry, TLC, HPLC methods.Alkaloid was found in all tissue cultures and E. intermedia organs. Total alkaloids were higher in roots and some shoots cultures than of ephedra shoots and seeds, cultures shoots and callus. The ephedrine and pseudoephedrine were higher in ephedra roots than in ephedra shoots and seeds, all callusculturesand some shoots cultures but they balancedin roots with shoots cultures of 15 mgl-1 NAA supplemented with 10 mgl-1 Kn.This study can be utilized to initiate multiple shoots culture of Ephedrathat may provide a good source of pharmacologically active constituents.
VL  - 3
IS  - 1
ER  -

Copy | Download