In Vitro Shoot and Callus Induction and Alkaloid Contents of Ephedra intermedia (Schrenket) of Iran
Journal of Plant Sciences
Volume 3, Issue 1, February 2015, Pages: 1-8
Received: Dec. 12, 2014; Accepted: Dec. 18, 2014; Published: Jan. 19, 2015
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Azra Ataei Azimi, Associate professorof plant cell and developmental biology, Department of Biology, Saveh Branch, Islamic Azad University, Saveh, Iran
Babak Delnavaz Hashemloian, Associate professorof plant cell and developmental biology, Department of Biology, Saveh Branch, Islamic Azad University, Saveh, Iran
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Ephedra intermedia(Schrenket) isan evergreen shrub belongs to family Ephedraceae.The present study deals with Invitro callus induction, multiple shoots regeneration and alkaloid production of E.intermedia. Callus andmultiple shoots formation were initiated on MS medium supplementedwith different concentrations of 1-Naphthalene Acetic Acid (NAA)and Kinetin (Kn)hormonescombination. Alkaloid analysis was done by spectrophotometry, TLC, HPLC methods.Alkaloid was found in all tissue cultures and E. intermedia organs. Total alkaloids were higher in roots and some shoots cultures than of ephedra shoots and seeds, cultures shoots and callus. The ephedrine and pseudoephedrine were higher in ephedra roots than in ephedra shoots and seeds, all callusculturesand some shoots cultures but they balancedin roots with shoots cultures of 15 mgl-1 NAA supplemented with 10 mgl-1 Kn.This study can be utilized to initiate multiple shoots culture of Ephedrathat may provide a good source of pharmacologically active constituents.
Ma-Haung, Multiple Shoots, Ephedrine, Pseudoephedrine
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Azra Ataei Azimi, Babak Delnavaz Hashemloian, In Vitro Shoot and Callus Induction and Alkaloid Contents of Ephedra intermedia (Schrenket) of Iran, Journal of Plant Sciences. Vol. 3, No. 1, 2015, pp. 1-8. doi: 10.11648/j.jps.20150301.11
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