Detection of Bacterial and Fusarium wilt Pathogens in Cabbage by Multiplex PCR
Journal of Plant Sciences
Volume 3, Issue 4, August 2015, Pages: 185-190
Received: Sep. 11, 2014;
Accepted: Sep. 23, 2014;
Published: Jul. 14, 2015
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Umesha, S., Department of Studies in Biotechnology, University of Mysore, Manasagangotri, Mysore, Karnataka, India
Jyothi, N., Department of Studies in Biotechnology, University of Mysore, Manasagangotri, Mysore, Karnataka, India
Roohie, R. K., Department of Studies in Biotechnology, University of Mysore, Manasagangotri, Mysore, Karnataka, India
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Cabbage is one of the most important vegetable which is extensively cultivated in India and other countries. Fusarium yellows caused by Fusarium oxysporum f.sp. conglutinans is one of the most destructive diseases of cabbage and other crucifers. It causes severe loss in the yield in terms of both quantity and quality. The work on Fusarium oxysporum f.sp.conglutinans in India is very scanty. The aim of the present study was to isolate and study the morphological diversity and pathogenicity variability among F. oxysporum f.sp. conglutinans isolated from different cabbage fields. During the present work, an attempt was made to isolate, identify and characterize the Fusarium yellows of cabbage causing pathogen Fusarium oxysporum f.sp. conglutinans, a total of nine soil samples were collected from different fields out of which five were found to be positive for Fusarium oxysporum f.sp. conglutinans. The morphological, physiological characterization results of the present study including, pathogenicity reaction effectively demonstrated the confirmation of isolated pathogen as Fusarium oxysporum f.sp. conglutinans. A multiplex PCR method was developed for the easy detection of the Fusarium yellows and the black rot pathogen Xanthomonas campestris pv. campestris which in the initial stages produce symptoms which appear similar.
Cabbage, Cabbage Yellows, Multiplex PCR, Fusarium oxysporum f.sp. conglutinans
To cite this article
Roohie, R. K.,
Detection of Bacterial and Fusarium wilt Pathogens in Cabbage by Multiplex PCR, Journal of Plant Sciences.
Vol. 3, No. 4,
2015, pp. 185-190.
Roohie, R.K. and Umesha, S. 2012 Development of Multiplex PCR for the Specific Detection of Xanthomonas campestris pv. campestris in Cabbage and Correlation with Disease Incidence. Journal of Plant Pathology Microbriology 3:127
Waksman, S.A., Journal of Bacteriology. 1922 , 7:339-341.
Warcup, J.H., Nature, London. 1950, 166,117.
Doyle, J.J.; Doyle, J.L. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin, v.19, p.11-15, 1987.
Gabriel DW, De Feyter R (1992) RFLP analyses and gene tagging for bacterial identification and taxonomy. In: Molecular Plant Pathology. Vol.1, A Practical Approach. (S.J. Gurr, M.J.McPherson and D.J. Bowles,eds). IRL Press, Oxford. Pp. 51-66.
Sakthivel N, Mortensen CN & Mathur SB (2001) Detection of Xanthomonas oryzae pv. oryzae in artificially and naturally infected rice seeds andplants by molecular techniques. Applied Microbiology and Biotechnology56, 435–441
Woltz, S.S. and Jones,J.P. 1981. Nutritional requirements of Fusarium oxvsporum: Basis for a disease control system. In Fusarium: Diseases, Biology and Taxonomy (P.E. Nelson., T.A. Toussoun., and R.J. Cook, editions): 340-349. The Pennysylvania State University Press University Park and London.
Higgins, K.L., Arnold, A.E., Miadlikowska, J., Sarvate, S.D. and Lutzoni F. 2003. Phylogenetic relationships, host affinity and geographic structure of boreal and arctic endophytes from three major plant lineages. Molecular Phylogenetics Evolution. 42:543–555.