Optimizing of Synthetic Seed of Malling apple (Malusdomestica) Rootstocks cv. M26 and cv. MM106
Journal of Plant Sciences
Volume 4, Issue 3, June 2016, Pages: 46-51
Received: Sep. 10, 2015; Accepted: Apr. 14, 2016; Published: May 11, 2016
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Authors
Zarinkamar Elham, Department of Biology, School of Basic Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran
Nejad Satari Taher, Department of Biology, School of Basic Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran
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Abstract
Malling Increasing demands for apple across the world requires its rapid production. Agricultural biotechnology has met this need and has prevented some problems during adolescence period and long generation. We investigated embryonic and non-embryonic callus through cultivating apical and lateral buds on different treatments with MS base culture and BAP (0-2.5 mg/l) and IBA (0-5 mg/l) hormones Kin and NAA hormones. The highest percent of embryogenesis related to T11 treatment (IBA 4 mg/l, BAP 1 mg/l) and T23 (IBA 4 mg/l, BAP 2.5 mg/l). The embryos were in corpuscular stage. Then third sub-culture was performed and all calluses and embryos were delivered to their corresponding treatments. K5 (NAA 1 mg/l, Kin 0.5 mg/l) and K8 (NAA 3 mg/l, Kin 1 mg/l) yielded the highest amount of callus (5.3 mm). The embryos remained in corpuscular stage and they did not show reproductive ability and developmental process. After preparation of artificial seed no regeneration was seen. By embryogenesis of MM.106 and M.26, the purpose of this research is improving a protocol for generation of two somatic cultivars.
Keywords
Malling, Synthetic Seed, Tissue Culture
To cite this article
Zarinkamar Elham, Nejad Satari Taher, Optimizing of Synthetic Seed of Malling apple (Malusdomestica) Rootstocks cv. M26 and cv. MM106, Journal of Plant Sciences. Vol. 4, No. 3, 2016, pp. 46-51. doi: 10.11648/j.jps.20160403.13
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