Morpho-Molecular Screening for Bacterial Leaf Blight Resistance in Some Rice Lines and Varieties

M. H. M. Mubassir; Khondoker M. Nasiruddin; Nazmul Hoque Shahin; Shamsun Nahar Begum; Manas Kanti Saha; A. Q. M. Bazlur Rashid

doi:doi:10.11648/j.jps.20160406.13

Morpho-Molecular Screening for Bacterial Leaf Blight Resistance in Some Rice Lines and Varieties

M. H. M. Mubassir, Khondoker M. Nasiruddin, Nazmul Hoque Shahin, Shamsun Nahar Begum, Manas Kanti Saha, A. Q. M. Bazlur Rashid

Published in Journal of Plant Sciences (Volume 4, Issue 6)

Received: 18 October 2016 Accepted: 27 October 2016 Published: 16 November 2016

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Abstract

Bacterial leaf blight (BLB) is one of the major and oldest diseases of rice. The best cost effective management of this disease is the development of resistant cultivars. Morphological and molecular screening are widely used approach in the recent scenario for identifying BLB resistant cultivar. In present study, two experiments were carried out to identify the BLB resistant rice lines and varieties. An active strain of Xanthomonas oryzae pv. oryaze (Xoo.) BXO-09 was used for the inoculation experiments. Then, morphological rating was done to find out the resistant rice lines and varieties whereas three RFLP primers (RG136, RG556 and pTA248) were used for identifying the BLB resistant genes. Through morphological analysis, the rice line RC251 was recorded as susceptible where the rest of the rice lines resulted moderately susceptible or moderately resistant. Among the rice varieties, BR-26, BRRI Dhan31, IRBB5, IRBB21, IRBB60, IRBB65, and Kumragur were recorded as resistant where BR-11, Binadhan-8, and Binadhan-10 were found highly susceptible to BLB. In case of RFLP marker based analysis, a specific type of banding pattern was observed for IRBB5 for RG556 primer which indicated the presence of xa5 gene. For RG136 primer 8 kb single band was formed for different lines and varieties whereas only IRBB60 showed 2 fragments of 8 kb and 5 kb which also indicated the presence of xa13 gene. Finally, DNA amplification of 27 rice lines and varieties using pTA248 primer showed the band at 4 kb position for the BLB resistant lines and varieties and 3.7 kb position for the susceptible lines and varieties. The findings of morpho-molecular characterization information could be useful to the breeders for further planning of developing BLB resistant cultivars.

Published in	Journal of Plant Sciences (Volume 4, Issue 6)
DOI	10.11648/j.jps.20160406.13
Page(s)	146-152
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Bacterial Leaf Blight (BLB), Restriction Fragment Length Polymorphism (RFLP), Resistance

References

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[16]	Ullah, I., et al., Detection of bacterial blight resistance genes in basmati rice landraces. Genetics and Molecular Research, 2012. 11(3): p. 1960-1966.
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APA Style

M. H. M. Mubassir, Khondoker M. Nasiruddin, Nazmul Hoque Shahin, Shamsun Nahar Begum, Manas Kanti Saha, et al. (2016). Morpho-Molecular Screening for Bacterial Leaf Blight Resistance in Some Rice Lines and Varieties. Journal of Plant Sciences, 4(6), 146-152. https://doi.org/10.11648/j.jps.20160406.13

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ACS Style

M. H. M. Mubassir; Khondoker M. Nasiruddin; Nazmul Hoque Shahin; Shamsun Nahar Begum; Manas Kanti Saha, et al. Morpho-Molecular Screening for Bacterial Leaf Blight Resistance in Some Rice Lines and Varieties. J. Plant Sci. 2016, 4(6), 146-152. doi: 10.11648/j.jps.20160406.13

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AMA Style

M. H. M. Mubassir, Khondoker M. Nasiruddin, Nazmul Hoque Shahin, Shamsun Nahar Begum, Manas Kanti Saha, et al. Morpho-Molecular Screening for Bacterial Leaf Blight Resistance in Some Rice Lines and Varieties. J Plant Sci. 2016;4(6):146-152. doi: 10.11648/j.jps.20160406.13

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@article{10.11648/j.jps.20160406.13,
  author = {M. H. M. Mubassir and Khondoker M. Nasiruddin and Nazmul Hoque Shahin and Shamsun Nahar Begum and Manas Kanti Saha and A. Q. M. Bazlur Rashid},
  title = {Morpho-Molecular Screening for Bacterial Leaf Blight Resistance in Some Rice Lines and Varieties},
  journal = {Journal of Plant Sciences},
  volume = {4},
  number = {6},
  pages = {146-152},
  doi = {10.11648/j.jps.20160406.13},
  url = {https://doi.org/10.11648/j.jps.20160406.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jps.20160406.13},
  abstract = {Bacterial leaf blight (BLB) is one of the major and oldest diseases of rice. The best cost effective management of this disease is the development of resistant cultivars. Morphological and molecular screening are widely used approach in the recent scenario for identifying BLB resistant cultivar. In present study, two experiments were carried out to identify the BLB resistant rice lines and varieties. An active strain of Xanthomonas oryzae pv. oryaze (Xoo.) BXO-09 was used for the inoculation experiments. Then, morphological rating was done to find out the resistant rice lines and varieties whereas three RFLP primers (RG136, RG556 and pTA248) were used for identifying the BLB resistant genes. Through morphological analysis, the rice line RC251 was recorded as susceptible where the rest of the rice lines resulted moderately susceptible or moderately resistant. Among the rice varieties, BR-26, BRRI Dhan31, IRBB5, IRBB21, IRBB60, IRBB65, and Kumragur were recorded as resistant where BR-11, Binadhan-8, and Binadhan-10 were found highly susceptible to BLB. In case of RFLP marker based analysis, a specific type of banding pattern was observed for IRBB5 for RG556 primer which indicated the presence of xa5 gene. For RG136 primer 8 kb single band was formed for different lines and varieties whereas only IRBB60 showed 2 fragments of 8 kb and 5 kb which also indicated the presence of xa13 gene. Finally, DNA amplification of 27 rice lines and varieties using pTA248 primer showed the band at 4 kb position for the BLB resistant lines and varieties and 3.7 kb position for the susceptible lines and varieties. The findings of morpho-molecular characterization information could be useful to the breeders for further planning of developing BLB resistant cultivars.},
 year = {2016}
}

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TY - JOUR
T1 - Morpho-Molecular Screening for Bacterial Leaf Blight Resistance in Some Rice Lines and Varieties
AU - M. H. M. Mubassir
AU - Khondoker M. Nasiruddin
AU - Nazmul Hoque Shahin
AU - Shamsun Nahar Begum
AU - Manas Kanti Saha
AU - A. Q. M. Bazlur Rashid
Y1 - 2016/11/16
PY - 2016
N1 - https://doi.org/10.11648/j.jps.20160406.13
DO - 10.11648/j.jps.20160406.13
T2 - Journal of Plant Sciences
JF - Journal of Plant Sciences
JO - Journal of Plant Sciences
SP - 146
EP - 152
PB - Science Publishing Group
SN - 2331-0731
UR - https://doi.org/10.11648/j.jps.20160406.13
AB - Bacterial leaf blight (BLB) is one of the major and oldest diseases of rice. The best cost effective management of this disease is the development of resistant cultivars. Morphological and molecular screening are widely used approach in the recent scenario for identifying BLB resistant cultivar. In present study, two experiments were carried out to identify the BLB resistant rice lines and varieties. An active strain of Xanthomonas oryzae pv. oryaze (Xoo.) BXO-09 was used for the inoculation experiments. Then, morphological rating was done to find out the resistant rice lines and varieties whereas three RFLP primers (RG136, RG556 and pTA248) were used for identifying the BLB resistant genes. Through morphological analysis, the rice line RC251 was recorded as susceptible where the rest of the rice lines resulted moderately susceptible or moderately resistant. Among the rice varieties, BR-26, BRRI Dhan31, IRBB5, IRBB21, IRBB60, IRBB65, and Kumragur were recorded as resistant where BR-11, Binadhan-8, and Binadhan-10 were found highly susceptible to BLB. In case of RFLP marker based analysis, a specific type of banding pattern was observed for IRBB5 for RG556 primer which indicated the presence of xa5 gene. For RG136 primer 8 kb single band was formed for different lines and varieties whereas only IRBB60 showed 2 fragments of 8 kb and 5 kb which also indicated the presence of xa13 gene. Finally, DNA amplification of 27 rice lines and varieties using pTA248 primer showed the band at 4 kb position for the BLB resistant lines and varieties and 3.7 kb position for the susceptible lines and varieties. The findings of morpho-molecular characterization information could be useful to the breeders for further planning of developing BLB resistant cultivars.
VL - 4
IS - 6
ER -

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Author Information

M. H. M. Mubassir

Department of Biotechnology, Bangladesh Agricultural University, Mymensingh, Bangladesh
Khondoker M. Nasiruddin

Department of Biotechnology, Bangladesh Agricultural University, Mymensingh, Bangladesh
Nazmul Hoque Shahin

Bangladesh Institute of Nuclear Agriculture, Bangladesh Agricultural University, Mymensingh, Bangladesh
Shamsun Nahar Begum

Bangladesh Institute of Nuclear Agriculture, Bangladesh Agricultural University, Mymensingh, Bangladesh
Manas Kanti Saha

Department of Biotechnology, Bangladesh Agricultural University, Mymensingh, Bangladesh
A. Q. M. Bazlur Rashid

Department of Plant Pathology, Bangladesh Agricultural University, Mymensingh, Bangladesh

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APA Style

M. H. M. Mubassir, Khondoker M. Nasiruddin, Nazmul Hoque Shahin, Shamsun Nahar Begum, Manas Kanti Saha, et al. (2016). Morpho-Molecular Screening for Bacterial Leaf Blight Resistance in Some Rice Lines and Varieties. Journal of Plant Sciences, 4(6), 146-152. https://doi.org/10.11648/j.jps.20160406.13

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ACS Style

M. H. M. Mubassir; Khondoker M. Nasiruddin; Nazmul Hoque Shahin; Shamsun Nahar Begum; Manas Kanti Saha, et al. Morpho-Molecular Screening for Bacterial Leaf Blight Resistance in Some Rice Lines and Varieties. J. Plant Sci. 2016, 4(6), 146-152. doi: 10.11648/j.jps.20160406.13

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AMA Style

M. H. M. Mubassir, Khondoker M. Nasiruddin, Nazmul Hoque Shahin, Shamsun Nahar Begum, Manas Kanti Saha, et al. Morpho-Molecular Screening for Bacterial Leaf Blight Resistance in Some Rice Lines and Varieties. J Plant Sci. 2016;4(6):146-152. doi: 10.11648/j.jps.20160406.13

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@article{10.11648/j.jps.20160406.13,
  author = {M. H. M. Mubassir and Khondoker M. Nasiruddin and Nazmul Hoque Shahin and Shamsun Nahar Begum and Manas Kanti Saha and A. Q. M. Bazlur Rashid},
  title = {Morpho-Molecular Screening for Bacterial Leaf Blight Resistance in Some Rice Lines and Varieties},
  journal = {Journal of Plant Sciences},
  volume = {4},
  number = {6},
  pages = {146-152},
  doi = {10.11648/j.jps.20160406.13},
  url = {https://doi.org/10.11648/j.jps.20160406.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jps.20160406.13},
  abstract = {Bacterial leaf blight (BLB) is one of the major and oldest diseases of rice. The best cost effective management of this disease is the development of resistant cultivars. Morphological and molecular screening are widely used approach in the recent scenario for identifying BLB resistant cultivar. In present study, two experiments were carried out to identify the BLB resistant rice lines and varieties. An active strain of Xanthomonas oryzae pv. oryaze (Xoo.) BXO-09 was used for the inoculation experiments. Then, morphological rating was done to find out the resistant rice lines and varieties whereas three RFLP primers (RG136, RG556 and pTA248) were used for identifying the BLB resistant genes. Through morphological analysis, the rice line RC251 was recorded as susceptible where the rest of the rice lines resulted moderately susceptible or moderately resistant. Among the rice varieties, BR-26, BRRI Dhan31, IRBB5, IRBB21, IRBB60, IRBB65, and Kumragur were recorded as resistant where BR-11, Binadhan-8, and Binadhan-10 were found highly susceptible to BLB. In case of RFLP marker based analysis, a specific type of banding pattern was observed for IRBB5 for RG556 primer which indicated the presence of xa5 gene. For RG136 primer 8 kb single band was formed for different lines and varieties whereas only IRBB60 showed 2 fragments of 8 kb and 5 kb which also indicated the presence of xa13 gene. Finally, DNA amplification of 27 rice lines and varieties using pTA248 primer showed the band at 4 kb position for the BLB resistant lines and varieties and 3.7 kb position for the susceptible lines and varieties. The findings of morpho-molecular characterization information could be useful to the breeders for further planning of developing BLB resistant cultivars.},
 year = {2016}
}

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