Journal of Plant Sciences
Volume 4, Issue 6, December 2016, Pages: 153-164
Received: Oct. 13, 2016;
Accepted: Oct. 31, 2016;
Published: Nov. 23, 2016
Views 4065 Downloads 232
Md. Rezwan Molla, Molecular Biology Laboratory, Plant Genetic Resources Centre, Bangladesh Agricultural Research Institute (BARI), Gazipur, Bangladesh
Iftekhar Ahmed, Molecular Biology Laboratory, Plant Genetic Resources Centre, Bangladesh Agricultural Research Institute (BARI), Gazipur, Bangladesh
Md. Motiar Rohman, Molecular Breeding Laboratory, Plant Breeding Division, BARI, Gazipur, Bangladesh
Md. Amjad Hossain, Molecular Biology Laboratory, Plant Genetic Resources Centre, Bangladesh Agricultural Research Institute (BARI), Gazipur, Bangladesh
Md. Aziz Zilani Chowdhury, Crops Division, Bangladesh Agricultural Research Council (BARC), Farmgate, Dhaka, Bangladesh
Microsatellite combines several features of an ultimate molecular marker and they are used increasingly in various plant genetic studies and applications. Characterization of mungbean genotypes on the basis of DNA fingerprinting has become an efficient tool to link genotypic variation. This work is reporting the utilization of a small set of five previously developed mungbean microsatellite (SSR) markers for the identification and discrimination of six HYVs and 36 landraces. All five microsatellite markers were found to be polymorphic. Variation was found in number of alleles, allele frequency, observed and expected heterozygosity. Using five primers across 42 genotypes a total of 20 alleles with an average number of 4 alleles per locus were found of which GBssr-MB91 showed highest number of alleles (6) (size ranging from 135 to 152 bp) followed by 4 alleles (from 160 to 176 bp and 175 to 195 bp) and 3 alleles (from 264 to 282 bp and 283 to 304 bp) were detected at the loci LR7322B, LR7323A, LR7323B and GBssr-MB77, respectively. The narrow genetic base could be one of the reasons for the low yield of polymorphic markers in the study. The primer GBssr-MB91 also yielded highest number of PIC value (0.803). Genetic differentiation (Fst) values were found in the ranges 0.443 to 0.747 with an average of 0.686 and gene flow (Nm) values ranged from 0.085 to 0.314 with an average of 0.237. Over all Nei’s genetic distance value (D) obdervedfrom nil to 2.706 among 861accessions pair resulting as a means of permutation combination of 42 mungbean genotypes. The UPGMA dendogram based on Nei’s genetic distance separated the genotypes, BARI mung-1 and BD6906 from other 40 genotype. Out of 42 genotypes, 36 genotypes were identified with at least one and/or combination of 4 primers.
Md. Rezwan Molla,
Md. Motiar Rohman,
Md. Amjad Hossain,
Md. Aziz Zilani Chowdhury,
Genetic Diversity Analysis and DNA Fingerprinting of Mungbean (Vigna radiata L.) Genotypes Using SSR Markers, Journal of Plant Sciences.
Vol. 4, No. 6,
2016, pp. 153-164.
BBS (Bangladesh Bureau of Statistics). 2008. Handbook of agricultural statistics, Ministry of Planning. Govt. People’s Republic of Bangladesh, Dhaka, p. 14.
Mondal, M. M. A. 2007. A study of source-sink relationship in mungbean. Ph. D. Dissertation, Department of Crop Botany. Bangladesh Agricultural University, Mymensingh, p. 21.
Sony SK, Habib MA, Islam MN. 2012. Genetic diversity analysis of thirteen mungbean (Vignaradiata (L.) Wilczek) cultivars using rapd markers. Bangladesh J Bot 41(2): 169-175.
Karp A, Kresovichi S, Bhat KV, Ayad WG Hodgkin T. 1997. Molecular tools in plant genetic resourcs conservation: a guid to the technologies: IPGR technical bull. No. 2. International Genetic Resrouces Institute, Rome, Italy.
Rahman L, Molla MR, Sultana S, Islam MN, Ahmed NU, Rahman MS, Nazim-ud-Dowla M, Shah-E-Alam M, Alam MS. 2006. Plant varieties of Bangladesh-morphological and molecular characterization for plant variety protection. Bangladesh J AgricSci, 33(2): 215-225.
Soller M, Beckmann JS. 1983. Genetic polymorphism in varietal identification and genetic improvement. TheorAppl Genet, 67(1): 25-33.
Williams JG, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV. 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res, 18(22): 6531-6535.
Zietkiewicz E, Rafalshi A Labuda D. 1994. Genome fingerprinting by simple sequence repeat (SSR)- anchored polymerase chain reaction amplification. Genom, 20(2): 176-183.
Becker J, Heun M. 1995. Barley microsatellites: allele variation and mapping. Plant MolBiol, 27(4): 835-845.
Dikshit HK, Jhang T, Singh NK, Koundal KR, Bansal KC, Chandra N, Tickoo JL, Sharma TR. 2007. Genetic differentiation of Vigna species by RAPD, URP and SSR markers. Biol Plant 51(3): 451-457.
Yoon MS, Lee J, Kim CY Baek HJ. 2007. Genetic relationships among cultivated and wild Vignaangularis (Willd.) OhwietOhashi and relatives from Korea based on AFLP markers. Genet Resour Crop Evol, 54(4): 875-883.
Kaga A, Yoon MS, Tomooka N, Vaughan DA. 2000. Collection of Vigna spp. and other legumes from the islands of southern Okinawa prefecture, Japan. In: Report to IPGRI and East Asia Plant Genetic Resources Coordinators. National Institute of Agrobiological Resources, Japan, pp. 2-25.
Ajibade SR, Weeden NF, Chite SM. 2000. Inter simple sequence repeat analysis of genetic relationships in the genus Vigna. Euphytica, 111 (1): 47-55.
Mailer RJ, Scarth R, Fristensky B. 1994. Discrimination among cultivars of rapeseed (Brassica napusL.) using DNA polymorphism amplified from arbitrary primers. TheorApplGenet, 87(6): 697-704.
Marshall P, Marchand MC, Lisieczko Z, Landry BS. 1994. A simple method to estimate the percentage of hybridity in canola (Brassica napus) F1 hybrids. TheorAppl Genet, 89(7-8): 853-858.
Bligh HFJ, Blackhall NW Edwards KJ, McClung AM. 1999. Using amplified fragment length polymorphisms and simple sequence length polymorphisms to identify cultivars of brown and white milled rice. Crop Sci, 39(6): 1715-1721.
Powell W, Machray GC, Provan J. 1996. Polymorphism revealed by simplesequence repeats. Trends Plant Sci, 1(7): 215-222.
Lagercrantz U, Ellegren H, Andersson L. 1993. The abundance of various polymorphic microsatellite motifs differs between plants and vertebrates. Nucleic Acids Res,21(5): 1111-1115.
Wu KS, Tanksley SD. 1993. Abundance, polymorphism and genetic mapping of microsatellites in rice. Mol Gen Genet MGG, 241(1-2): 225-235.
Islam MN, Molla MR, Rahman L. 2007. Microsatellite allele size profiling to identify and distinguish soybean cultivars in Bangladesh. ProgAgric, 18(1): 9-17.
Molla MR, Islam MN, Rahman L. 2007. DNA fingerprinting of maize (Zea mays linn.) cultivars of Bangladesh using SSR markers. Bangladesh J Crop Sci, 18(1): 63-72.
Rahman L, Islam MN, Rahman MS, Islam MS. 2008. Plant varieties of Bangladesh: morphological and molecular characterization. Published by Seed Wing, Ministry of Agriculture, Government of the People’s Republic of Bangladesh, Vol. 2, p. 300.
Bhuyan SI, Hossain MS, Islam MM, Begum SN, Urbi Z, Hossain MS. 2014. Molecular assessment of genetic diversity and relationship in selected mungbeangermplasm. Biotec, 13(3): 126-134.
Saghai-Maroof MA, Soliman KM, Jonsensan RA, Allard RW. 1984. Ribosomal spacer length polymorphism in barley: mendelian inheritance, chromosomal location and population dynamics. ProcNatlAcadSci, USA, 81: 8014-8018.
Rahman, L, Molla MR, Sultana S, Islam MN, Ahmed NU, Rahman MS, Nazim-ud-Dowla M. 2007. Plant varieties of Bangladesh: morphological and molecular characterization. Published by Seed Wing, Ministry of Agriculture, Government of the People’s Republic of Bangladesh, Vol. 1, p. 486.
Nash JHE. 1991. DNA frag, Version 3.03. Institute for biological sciences, National Research Council of Canada, Ottawa, Ontario, Canada.
Nei M. 1972. Genetic distance between populations. Am Nat, 106(949):283 292.
Yeh FC, Yang RC, Boyle T. 1999. POPGENE version 1.32, Microsoft window-base software for population genetic analysis, a quick user’s guide. University of Alberta. Center for International Forestry Research, Alberta, Canada.
Kumar SV, Tan SG, Quah SC, Yusoff K. 2002. Isolation of microsatellite markers in mungbean, Vignaradiata. MolEcol Notes, 2(2): 96-98.
Gwag, JG, Chung JW, Chung HK, Lee JH, Ma KH, Dixit A, Park YJ, Cho EG, Kim TS, Lee SH. 2006. Characterization of new microsatellite markers in mungbean, Vignaradiata (L.). MolEcol Notes, 6(4): 1132-1134.
Cao T, Duprez E, Borden KL, Freemont PS, Etkin LD. 1998. Ret finger protein is a normal component of PML nuclear bodies and interacts directly with PML. J Cell Sci, 111(10): 1319-1329.
Vos P, Hogers R, Bleeker M, Reijans M, Van de Lee T, Hornes M, Friters A, Pot J, Paleman J, Kuiper M, Zabeau M. 1995. AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res, 23(21): 4407- 4414.
Yu SB, Xu WJ, Vijayakumar CHM, Ali J, Fu BY, Xu JL, Jiang YZ, Marghirang R, Domingo J, Aquino C, Virmani SS. 2003. Molecular diversity and multilocus organization of the parental lines used in the International Rice Molecular Breeding Program. TheorAppl Genet, 108 (1): 131-140.
Nei M. 1973. Analysis of gene diversity in subdivided populations. ProcNatlAcadSci, USA 70(12): 3321-3323.
Levene H. 1949. On a matching problem arising in genetics. Ann Math Stat, 20(1): 91-94.
Song QJ, Quiley CV, Nelson RL, Carter TE, Boema HR, Strachan JL, Cregan PB. 1999. A selected set of trinucleotide simple sequence repeat markers for soybean cultivar identification. Pl vari seeds, 12:207-220.