In vitro Plantlet Regeneration from Nodal Explant and Callus Induction of Vernonia amygdalina Delile
Journal of Plant Sciences
Volume 6, Issue 1, February 2018, Pages: 1-6
Received: Dec. 7, 2016; Accepted: Oct. 16, 2017; Published: Dec. 20, 2017
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Authors
Chen Mei-Yin, Department of Plant Science and Environmental Ecology, Faculty of Science and Resource Technology, University Malaysia Sarawak, Kota Samarahan, Malaysia
Hamsawi Sani, Department of Plant Science and Environmental Ecology, Faculty of Science and Resource Technology, University Malaysia Sarawak, Kota Samarahan, Malaysia
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Abstract
Vernonia amygdalina is a vegetable and medicinal plant used to treat various ailments such as diabetes, malaria, gastrointestinal disorders, and parasitic infections. The present study investigated the effect of supplementing different concentrations of 6-benzylaminopurine (BAP) and 1-naphthaleneacetic acid (NAA) either alone or in combination to Murashige and Skoog (MS) medium on in vitro plantlet regeneration of V. amygdalina from nodal explants. Control treatment without plant growth regulators was ideal for in vitro plantlet regeneration of V. amygdalina. In vitro plantlets regenerated from nodal explants supplemented with BAP and/or NAA showed growth abnormalities including chlorosis, basal callus, and excessive adventitious rooting. Callus cultures were induced from leaf explants on MS medium supplemented with different concentrations of BAP, NAA, and 2,4-dichlorophenoxyacetic acid (2,4-D) either alone or in combination. Maximum callus induction frequency (100%) was recorded in leaf explants cultured on MS medium supplemented with 0.5 – 2.0 mg/L 2,4-D. Fresh weight of calli increased up to 11-fold when treated with 0.5 mg/L 2,4-D after 8 weeks of culture.
Keywords
Vernonia amygdalina, Plantlet Regeneration, Callus
To cite this article
Chen Mei-Yin, Hamsawi Sani, In vitro Plantlet Regeneration from Nodal Explant and Callus Induction of Vernonia amygdalina Delile, Journal of Plant Sciences. Vol. 6, No. 1, 2018, pp. 1-6. doi: 10.11648/j.jps.20180601.11
Copyright
Copyright © 2017 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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