In vitro Plantlet Regeneration from Nodal Explant and Callus Induction of Vernonia amygdalina Delile
Journal of Plant Sciences
Volume 6, Issue 1, February 2018, Pages: 1-6
Received: Dec. 7, 2016; Accepted: Oct. 16, 2017; Published: Dec. 20, 2017
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Chen Mei-Yin, Department of Plant Science and Environmental Ecology, Faculty of Science and Resource Technology, University Malaysia Sarawak, Kota Samarahan, Malaysia
Hamsawi Sani, Department of Plant Science and Environmental Ecology, Faculty of Science and Resource Technology, University Malaysia Sarawak, Kota Samarahan, Malaysia
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Vernonia amygdalina is a vegetable and medicinal plant used to treat various ailments such as diabetes, malaria, gastrointestinal disorders, and parasitic infections. The present study investigated the effect of supplementing different concentrations of 6-benzylaminopurine (BAP) and 1-naphthaleneacetic acid (NAA) either alone or in combination to Murashige and Skoog (MS) medium on in vitro plantlet regeneration of V. amygdalina from nodal explants. Control treatment without plant growth regulators was ideal for in vitro plantlet regeneration of V. amygdalina. In vitro plantlets regenerated from nodal explants supplemented with BAP and/or NAA showed growth abnormalities including chlorosis, basal callus, and excessive adventitious rooting. Callus cultures were induced from leaf explants on MS medium supplemented with different concentrations of BAP, NAA, and 2,4-dichlorophenoxyacetic acid (2,4-D) either alone or in combination. Maximum callus induction frequency (100%) was recorded in leaf explants cultured on MS medium supplemented with 0.5 – 2.0 mg/L 2,4-D. Fresh weight of calli increased up to 11-fold when treated with 0.5 mg/L 2,4-D after 8 weeks of culture.
Vernonia amygdalina, Plantlet Regeneration, Callus
To cite this article
Chen Mei-Yin, Hamsawi Sani, In vitro Plantlet Regeneration from Nodal Explant and Callus Induction of Vernonia amygdalina Delile, Journal of Plant Sciences. Vol. 6, No. 1, 2018, pp. 1-6. doi: 10.11648/j.jps.20180601.11
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Aletor, O., Oshodi, A. A., and Ipinmoroti, K. (2002) Chemical composition of common leafy vegetables and functional properties of their leaf protein concentrates. Food Chemistry, 78 (1), 63-68.
Amin, S., Kaloo, Z. A., Singh, S., and Altaf, T, (2013) Micropropagation of medicinally important plant species of family Asteraceae – A review. International Journal of Recent Scientific Research, 4 (8), 1296-1303.
Bairu, M. W., and Kane, M. E. (2011) Physiological and developmental problems encountered by in vitro cultured plants. Plant Growth Regulators, 63, 101-103.
Bairu, M. W., Stirk, W. A., Dolezal, K., and Van Staden, J. (2007) Optimizing the micropropagation protocol for the endangered Aloe polyphylla: can meta-topolin and its derivatives served as replacement for benzyladenine and zeatin. Plant Cell Tissue and Organ Culture, 90, 15-23.
Bakar, D. A., Ahmed, B. A., and Taha, R. M. (2014) In vitro callus induction and plant regeneration of Celosia argentea – An important medicinal plant. Brazilian Archives of Biology and Technology, 57 (6), 860-866.
Barbosa, L. M. P., de Paiva Neto, V. B., Dias, L. L. C., Festucci-Buselli, R. A., Alexandre, R. S., Iarema, L., Finger, F. L., and Otoni, W. C. (2013) Biochemical and morpho-anatomical analyses of strawberry vitroplants hyperhydric tissue affected by BA and gelling agents. Revista Ceres, 60 (2).
Bidarigh, S., and Azarpour, E. (2013) Evaluation of the effect of MS medium levels on rooting in micro cuttings of tea (Camellia sinensis L.) under in-vitro culture condition. Asian Research Publishing Network (ARPN) Journal of Agricultural and Biological Science, 8 (1), 24-28.
Boo, K. H., Cao, D. V., Pamplona, R. S., Lee, D., Riu, K. Z., and Lee, D. S. (2015) In vitro plant regeneration of Aster scaber via somatic embryogenesis. Bioscience, Biotechnology, and Biochemistry, 79 (5), 725-731.
Caraballo, M. G., Oramas, G. G., García, S. A., Cruz, E. A., Bravo, K. Q., Caligari, P. D. S., and Garcíia-González, R. (2010) Management of auxin-cytokinin interactions to improve micropropagation protocol of henequen (Agave fourcroydes Lem.). Chilean Journal of Agricultural Research, 70 (4), 545-551.
Chakrabarty, D., Park, S. Y., Ali, M. B., Shin, K. S., and Paek, K. Y. (2005) Hyperhydricity in apple: ultrastructural and physiological aspects. Tree Physiology, 26, 377-388.
Chen, X., Qu, Y., Sheng, L., Liu, J., Huang, H., and Xu, L. (2014) A simple method suitable to study de novo organogenesis. Frontiers in Plant Science, 5, 208.
Daffalla, H. M., Abdellatef, E., and Khalafalla, M. (2014) Callogenesis and rhizogenesis in Vernonia amygdalina leaf: step for secondary metabolites production. Proceedings of 5th International Workshop Biotechnology and its Role in Economic Development in the Arab World.
Debergh, P. C. (1983) Effects of agar brand and concentrations on the tissue culture medium. Physiologia Plantarum, 59, 270-276.
Fomum, F. U. (2004) Vernonia amgydalina Delile. In Grubben, G. J. H. and Denton, O. A. (Eds.), Plant Resources of Tropical Africa 2: Vegetables. Netherlands: PROTA Foundation/Backhuys Publishers/CTA, pp 543-546.
Gümüşçü, A., Çöçü, S., Uranbey, S., Ipek, A., Çalıskan, M., and Arslan, N. (2008). In vitro micro-propagation of endangered ornamental plant-Neotchihatchewia isatidea (Boiss.) Rauschert. African Journal of Biotechnology, 7 (3), 234-238.
Huffman, M. A., and Seifu, M. (1989) Observations on the illness and consumption of a possibly medicinal plant Vernonia amgydalina (Del.), by a wild chimpanzee in the Mahale Mountains National Park, Tanzania. Primates, 30 (1), 51-63.
Hussain, M. S., Fareed, S., Ansari, S., Rahman, M. A., Ahmad, I. Z., and Saeed, M. (2012) Current approaches toward production of secondary plant metabolites. Journal of Pharmacy and BioAllied Sciences, 4 (1), 10-20.
Ivanova, M., Novák, O., Strnad, M., and Van Staden, J. (2006) Endogenous cytokinins in shoots of Aloe polyphylla cultured in vitro in relation to hyperhydricity, exogenous cytokinins and gelling agents. Plant Growth Regulation, 50 (2-3), 219-230.
Khalafalla, M. M., Abdellatef, E., Daffalla, H. M., Nassrallah, A. A., Aboul-Enein, K. M., Lightfoot, D. A., Cocchetto, A., and El-Shemy, H. A. (2009) Antileukemic activity from root cultures from Vernonia amygdalina. Journal of Medicinal Plants Research, 556-562.
Khalafalla, M. M., Elgaali, E. I., and Ahmed, M. M. (2007) In vitro multiple shoot regeneration from nodal explants of Vernonia amygdalina – An important medicinal plant. Proceedings from African Crop Science Conference.
Koshimizu, K., Ohigashi, H., and Huffman, M. A. (1994) Use of Vernonia amygdalina by wild chimpanzee: Possible roles of its bitter and related constituents. Physiology and Behaviour, 56 (6), 1209-1216.
Maheshwari, P., Songara, B., Kumar, S., Jain, P., Srivastava, K., and Kumar, A. (2007) Alkaloid production in Vernonia cinerea: Callus, cells suspension and root cultures. Biotechnology Journal, 2 (8), 1026-1032.
Oboh, G. (2006) Nutritive value and haemolytic properties (in vitro) of the leaves of Vernonia amygdalina on human erythrocyte. Nutrition and health, 18 (2), 151-160.
Ohigashi, H., Huffman, M. A., Izutsu, D., Koshimizu, K., Kawanaka, M. Sugiyama, H., Kirby, G. C., Warhurst, D. C., Allen, D., Wright, C. W., David Phillipson, J., Timon-David, P., Delmas, F., Elias, R., and Balansard, G. (1994) Toward the chemical ecology of medicinal plant use in chimpanzees: The case of Vernonia amgydalina, a plant used by wild chimpanzees possibly for parasite-related diseases. Journal of Chemical Ecology, 20 (3), 541-553.
Opabode, J. T., and Adebooye, O. C. (2005) Application of biotechnology for the improvement of Nigerian indigenous leaf vegetables. African Journal of Biotechnology, 4 (3), 138-142.
Rao, S. R., and Ravishankar, G. A. (2002) Plant cell cultures: Chemical factories of secondary metabolites. Biotechnology Advances, 20 (2), 101-153.
Shimizu-Sato, S., Tanaka, M., and Mori, H. (2009) Auxin-cytokinin interactions in the control of shoot branching. Plant Molecular Biology, 69, 429-435.
Simona, L., Cerasela, P., Alexandru, L., and Maria, B. (2012) Influence of growth regulators on morphogenetic processes under in vitro condition. Journal of Horticulture, Forestry and Biotechnology, 16 (2), 197-202.
Smetanska, I. (2008) Production of secondary metabolites using plant cell cultures. Advances in Biochemical Engineering and Biotechnology, 111, 187-228.
Tanaka, M., Takei, K., Kojima, M., Sakakibara, H., and Mori, H. (2006) Auxin controls local cytokinin biosynthesis in the nodal stem in apical dominance. The Plant Journal, 45 (6), 1028-1036.
Toyang, N. J., and Verpoorte, R. (2013) A review of the medicinal potentials of plants of the genus Vernonia (Asteraceae). Journal of Ethnopharmacology, 146 (3), 681-723.
Verpoorte, R., Contin, A., and Memelink, J. (2002) Biotechnology for the production of plant secondary metabolites. Phytochemistry Reviews, 1 (1), 13-25.
Vinothkumar, D., Murugavelh, S., and Senthilkumar, M. (2011) Clonal propagation of Wattakaka volubilis through nodal explant culture. Ceylon Journal of Science (Biological Science), 40 (1), 53-58.
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