Journal of Plant Sciences

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Effect of Different Carbon Sources on in vitro Shoot Regeneration and Multiplication of Cissampelos pareira (L.) - An Important Medicinal Plant

Received: 24 September 2019    Accepted: 17 October 2019    Published: 28 October 2019
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Abstract

An efficient in vitro protocol for shoot regeneration and production of multiple shoots of Cissampelos pareira (L) was developed. In the present study, the effect of different carbon sources, sucrose, fructose, maltose and glucose was investigated on in vitro shoot regeneration by using axillary bud of Cissampelos pareira (L). The frequency, growth and multiplication rate were highly influenced by the type and concentration of carbon sources used. The highest number of shoots (8.06±0.11) was observed on MS medium supplemented with 2% fructose and along with BAP 1.0mg/l and NAA 0.5mg/l and regeneration frequency 95%. The least number of shoots were obtained on MS medium supplemented with 4% maltose and (3.4±0.10) shoots and glucose (3.26±0.11). Among the different carbon sources used in the present study, fructose 2% proved to be a better choice for multiple shoot regeneration followed by Sucrose, maltose, and glucose from axillary bud explants of Cissampelos pareira. In vitro shoots were then excised from the shoot clumps and transferred to the rooting medium containing NAA, IBA (0.5-3.0mg/l). The well-rooted plantlets were then separated from the culture tubes and transferred into sterile soil and vermiculate (1:1) in the greenhouse. Finally, the hardened plants were transferred to the field environment for utmost survivability.

DOI 10.11648/j.jps.20190706.11
Published in Journal of Plant Sciences (Volume 7, Issue 6, December 2019)
Page(s) 131-136
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Axillary Bud Explants, Carbon Sources, BAP-6-Benzyl Amino Purine, NAA-1-Napthalene Acetic Acid, Micro Propagation, Cissampelos pareira

References
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Author Information
  • Department of Biotechnology, School of Herbal Studies and Naturo Sciences, Dravidian University, Kuppam, A. P. India

  • Department of Biotechnology, School of Herbal Studies and Naturo Sciences, Dravidian University, Kuppam, A. P. India

  • Department of Biotechnology, School of Herbal Studies and Naturo Sciences, Dravidian University, Kuppam, A. P. India

  • Department of Biotechnology, School of Herbal Studies and Naturo Sciences, Dravidian University, Kuppam, A. P. India

  • Department of Biotechnology, School of Herbal Studies and Naturo Sciences, Dravidian University, Kuppam, A. P. India

  • Department of Biotechnology, School of Herbal Studies and Naturo Sciences, Dravidian University, Kuppam, A. P. India

Cite This Article
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    Cingithalam Munaswamy Reddy Narendra Reddy, Palyam Venkatesh Chaithanya Lakshmi, Varagiri Narayanachetty Swetha Prasuna, Mustrahally Raja Gopal, Gunda Sudhasri Purna, et al. (2019). Effect of Different Carbon Sources on in vitro Shoot Regeneration and Multiplication of Cissampelos pareira (L.) - An Important Medicinal Plant. Journal of Plant Sciences, 7(6), 131-136. https://doi.org/10.11648/j.jps.20190706.11

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    Cingithalam Munaswamy Reddy Narendra Reddy; Palyam Venkatesh Chaithanya Lakshmi; Varagiri Narayanachetty Swetha Prasuna; Mustrahally Raja Gopal; Gunda Sudhasri Purna, et al. Effect of Different Carbon Sources on in vitro Shoot Regeneration and Multiplication of Cissampelos pareira (L.) - An Important Medicinal Plant. J. Plant Sci. 2019, 7(6), 131-136. doi: 10.11648/j.jps.20190706.11

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    AMA Style

    Cingithalam Munaswamy Reddy Narendra Reddy, Palyam Venkatesh Chaithanya Lakshmi, Varagiri Narayanachetty Swetha Prasuna, Mustrahally Raja Gopal, Gunda Sudhasri Purna, et al. Effect of Different Carbon Sources on in vitro Shoot Regeneration and Multiplication of Cissampelos pareira (L.) - An Important Medicinal Plant. J Plant Sci. 2019;7(6):131-136. doi: 10.11648/j.jps.20190706.11

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  • @article{10.11648/j.jps.20190706.11,
      author = {Cingithalam Munaswamy Reddy Narendra Reddy and Palyam Venkatesh Chaithanya Lakshmi and Varagiri Narayanachetty Swetha Prasuna and Mustrahally Raja Gopal and Gunda Sudhasri Purna and Bathula Srinivas},
      title = {Effect of Different Carbon Sources on in vitro Shoot Regeneration and Multiplication of Cissampelos pareira (L.) - An Important Medicinal Plant},
      journal = {Journal of Plant Sciences},
      volume = {7},
      number = {6},
      pages = {131-136},
      doi = {10.11648/j.jps.20190706.11},
      url = {https://doi.org/10.11648/j.jps.20190706.11},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.jps.20190706.11},
      abstract = {An efficient in vitro protocol for shoot regeneration and production of multiple shoots of Cissampelos pareira (L) was developed. In the present study, the effect of different carbon sources, sucrose, fructose, maltose and glucose was investigated on in vitro shoot regeneration by using axillary bud of Cissampelos pareira (L). The frequency, growth and multiplication rate were highly influenced by the type and concentration of carbon sources used. The highest number of shoots (8.06±0.11) was observed on MS medium supplemented with 2% fructose and along with BAP 1.0mg/l and NAA 0.5mg/l and regeneration frequency 95%. The least number of shoots were obtained on MS medium supplemented with 4% maltose and (3.4±0.10) shoots and glucose (3.26±0.11). Among the different carbon sources used in the present study, fructose 2% proved to be a better choice for multiple shoot regeneration followed by Sucrose, maltose, and glucose from axillary bud explants of Cissampelos pareira. In vitro shoots were then excised from the shoot clumps and transferred to the rooting medium containing NAA, IBA (0.5-3.0mg/l). The well-rooted plantlets were then separated from the culture tubes and transferred into sterile soil and vermiculate (1:1) in the greenhouse. Finally, the hardened plants were transferred to the field environment for utmost survivability.},
     year = {2019}
    }
    

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  • TY  - JOUR
    T1  - Effect of Different Carbon Sources on in vitro Shoot Regeneration and Multiplication of Cissampelos pareira (L.) - An Important Medicinal Plant
    AU  - Cingithalam Munaswamy Reddy Narendra Reddy
    AU  - Palyam Venkatesh Chaithanya Lakshmi
    AU  - Varagiri Narayanachetty Swetha Prasuna
    AU  - Mustrahally Raja Gopal
    AU  - Gunda Sudhasri Purna
    AU  - Bathula Srinivas
    Y1  - 2019/10/28
    PY  - 2019
    N1  - https://doi.org/10.11648/j.jps.20190706.11
    DO  - 10.11648/j.jps.20190706.11
    T2  - Journal of Plant Sciences
    JF  - Journal of Plant Sciences
    JO  - Journal of Plant Sciences
    SP  - 131
    EP  - 136
    PB  - Science Publishing Group
    SN  - 2331-0731
    UR  - https://doi.org/10.11648/j.jps.20190706.11
    AB  - An efficient in vitro protocol for shoot regeneration and production of multiple shoots of Cissampelos pareira (L) was developed. In the present study, the effect of different carbon sources, sucrose, fructose, maltose and glucose was investigated on in vitro shoot regeneration by using axillary bud of Cissampelos pareira (L). The frequency, growth and multiplication rate were highly influenced by the type and concentration of carbon sources used. The highest number of shoots (8.06±0.11) was observed on MS medium supplemented with 2% fructose and along with BAP 1.0mg/l and NAA 0.5mg/l and regeneration frequency 95%. The least number of shoots were obtained on MS medium supplemented with 4% maltose and (3.4±0.10) shoots and glucose (3.26±0.11). Among the different carbon sources used in the present study, fructose 2% proved to be a better choice for multiple shoot regeneration followed by Sucrose, maltose, and glucose from axillary bud explants of Cissampelos pareira. In vitro shoots were then excised from the shoot clumps and transferred to the rooting medium containing NAA, IBA (0.5-3.0mg/l). The well-rooted plantlets were then separated from the culture tubes and transferred into sterile soil and vermiculate (1:1) in the greenhouse. Finally, the hardened plants were transferred to the field environment for utmost survivability.
    VL  - 7
    IS  - 6
    ER  - 

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