Field Evaluation of Bread Wheat (Triticum aestivum L.) Genotypes for Stripe Rust (Puccinia striiformis W.) Resistance in Arsi Highlands, South -Eastern-Ethiopia
Journal of Plant Sciences
Volume 8, Issue 4, August 2020, Pages: 87-97
Received: May 21, 2020; Accepted: Jun. 28, 2020; Published: Aug. 31, 2020
Views 41      Downloads 21
Getnet Muche Abebele, Ethiopian Institute of Agricultural Research (EIAR), Wheat Regional Center of Excellence, KARC, Asella, Ethiopia
Merkuz Abera Admasu, Department of Plant Science, Bahir Dar University, Bahir Dar, Ethiopia
Bekele Hundie Agdu, Ethiopian Institute of Agricultural Research (EIAR), Wheat Regional Center of Excellence, KARC, Asella, Ethiopia
Article Tools
Follow on us
Wheat is one of the world's foremost crops where its production is growing yearly. However, the emerged virulent stripe rust races at one point of the world spread to the rest of wheat producing countries by wind as well as human travels and damaged popular resistant wheat cultivars thereby posed food insecurity. This study was carried out with the aim to identify possible sources of stripe rust resistance among Ethiopian bread wheat pipelines for durable resistance breeding. Twenty-eight advanced bread wheat pipelines, local susceptible and resistant check cultivars Kubsa and Wane respectively were field tested in randomized complete block design with three replications across two stripe rust hot-spot locations for their slow rusting characteristics. Slow rusting resistance at the adult-plant stage was assessed through the determination of final rust severity (FRS), average coefficient of infection (ACI), and relative area under disease progressive curve (rAUDPC). Among the twenty-eight, 24, 2 and 2 genotypes displayed high, moderate and low level of slow rusting over two locations respectively. The results revealed that wheat lines, ETBW- 8858, ETBW-8870, ETBW-8583, ETBW-8668, ETBW-8595, ETBW-8684, ETBW-9548, ETBW-9549, ETBW-9552, ETBW-9554, ETBW-9558, ETBW-9559, ETBW-9560, ETBW-875, ETBW-8802, ETBW-8862, ETBW-8804, ETBW-8896, ETBW-9556, ETBW-9557, ETBW-8991, ETBW-9486, ETBW-9556 and ETBW-9561 had low values of FRS, ACI and rAUDPC and were regarded as good slow rusting lines. Strong positive correlations were observed between different parameters of slow rusting. As compared with susceptible, resistant check variety and other test lines, three lines namely. ETBW-8684; ETBW-9558 and ETBW-8751 are high yielders and could be released for production. Twenty-four lines with high and moderate levels of slow rusting and expected to possess both major and minor resistance genes could be used for durable stripe rust resistance breeding in wheat. Nevertheless, the exact resistant genes contented in suggested lines shall be confirmed through seedling phenotyping and molecular approaches.
FRS, Inherit Resistant Genes, Adult Plat Resistance, Slow Rusting, Stripe Rust
To cite this article
Getnet Muche Abebele, Merkuz Abera Admasu, Bekele Hundie Agdu, Field Evaluation of Bread Wheat (Triticum aestivum L.) Genotypes for Stripe Rust (Puccinia striiformis W.) Resistance in Arsi Highlands, South -Eastern-Ethiopia, Journal of Plant Sciences. Vol. 8, No. 4, 2020, pp. 87-97. doi: 10.11648/j.jps.20200804.13
Copyright © 2020 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License ( which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Kilarquist E., Chen X. and Carter A. 2016. “Novel QTL for stripe rust resistance on Chromosomes 4A and 6B in soft white winter wheat cultivars,’’. Journal of Agronomy Vol 6. no. 1, Pp 16.
FAO. 2018. Food and agriculture data. Explore Data. Crops: Wheat [Online]. Food and Agricultural Organization of the United Nations (FAO). Available: [Accessed 28 May 2018].
CSA. 2018. Agricultural sample survey: Report on area and production of major crops, Central Statistical Agency, Addis Ababa, Ethiopia, 18 Pp.
Stubbs RW. 1985. Stripe rust. In: Roelfs AP, Bushnell WR (eds): The Cereal Rusts Cisease Methodology Manual, CIMMYT: Mexico, D. F. 46p. II. New York, pp 61–101.
Chen X. M. 2005. Epidemiology and control of stripe rust (Puccinia striiformis f. sp. tritici) on wheat. Canadian Journal of Plant Pathol. 27: 314-337.
Singh R. P., Huerta-Espino J., Bhavani S., Herrera-Foessel S. A., Singh D., Singh P. K., Velu G., Mason R. E., Jin Y., Njau P. and Crossa J. 2011. Race non-specific resistance to rust diseases in CIMMYT spring wheats. Euphytica. 179: 175-186.
Abdulbagiyeva S. A., Zamanov A. A. and Ahmadov N. S. 2014. Effects of yellow rust on yield: 2nd international wheat stripe rust symposium, regional cereal rust research center Izmir, Turkey. 7 Pp.
Beard C., Jayasena K., Thomas G. and Loughman R. 2007. Managing stripe rust and leaf rust of wheat. Department of Agriculture. Government of Western Australia.
Hailu Gebremariam. 1991. Bread wheat breeding and genetics research in Ethiopia. In: Hailu, G. M., Tanner, D. G., and Mengistu Huluka. (eds.). Wheat research in Ethiopia: a historical perspective. Addis Ababa. IAR/CIMMYT. pp. 73-93.
Worku Denbel, Bekele Hundie, Getaneh Woldeab, Endale Hailu, Bekele Kassa, Teklay Abebe, Daniel Kassa, Tilahun Bayissa, EbabuyeY, MekonenA., Ashenafi Gemechu, Seyoum Z., Nigussie D., BitewB., Handor F. and Girma Kassa. 2014. Monitoring wheat stripe rust in Ethiopia: The 2011 and 2012 trap nurseries and stripe rust virulence surveys. 2nd international wheat stripe rust symposium: Regional cereal rust research center Izmir, Turkey. Pp 27.
Ayele Badebo, Solomon Assefa and Fehrmann H. 2008a. Yellow rust resistance in advanced lines and commercial cultivars of bread wheat from Ethiopia. East African Journal of Science. 2: 29-34.
Wubit Dawit, Flath K. Weber E., Schumann E. Röder M. S. and Chen X. 2012. Postulation and mapping of seedling stripe rust resistance genes in Ethiopian bread wheat cultivars. Journal of Plant Pathology. 94: 2, 403-409.
Teklay Abebe. 2014. Variability in stripe rust occurrence in Tigray, Ethiopia. 2nd international wheat stripe rust symposium: regional cereal rust research center Izmir, Turkey. PP; 7.
Eshetu Bekele. 1985. Review of research on diseases of barley, tef and wheat in Ethiopia. In: Tsedeke Abate(ed). A review of crop protection research in Ethiopia. Proceedings of the first crop protection symposium. IAR, Addis Ababa Ethiopia. Pp 79-108.
Hovmøller M., Rodriguez-Algaba J., Thach T., Justesen A. F. and Hansen G. H. 2018. Report for Puccinia striiformis race analyses and molecular genotyping 2018. Global Rust Reference Center (GRRC), Aarhus University, Flakkebjerg, DK-4200 Slagelse, Denmark 10 February, 2018.
Hovmøller M. S., Rodriguez-Algaba J., Thach T. and Sørensen C. K. 2017. Race Typing of P. striiformis on Wheat. In: Sambasivam Periyannan (ed.), Wheat Rust Diseases: Methods and Protocols, Methods in Molecular Biology. 1659:. 29-40.
Solh M., Nazari K., Tadesse W. and Wellings C. R. 2012. The growing threat of stripe rust worldwide; In Proceedings of the Borlaug Global Rust Initiative (BGRI) Conference Beijing China.
Habtemariam Zegeye, Rasheed A., Makdis F., Ayele Badebo and Ogbonnaya F. C. 2014. Genome-wide association mapping for seedling and adult plant resistance to stripe rust in synthetic hexaploid wheat. PLOSE online J. 9: 98.
Atilaw Abebe., Zelalem Bishaw, Finisa Eticha, Solomon Gelalcha, Zerihun Tadesse, S. Aliye, Abdalla O., Asnake Fikre S. Ahmed and Silim S. 2014. Controlling wheat rusts and ensuring food security through deployment of resistant varieties in Ethiopia. 2nd international wheat stripe rust symposium regional cereal rust research center Izmir, Turkey. 14 Pp.
ICARDA. 2011. Strategies to reduce the emerging wheat stripe rust disease: Synthesis of a dialog between policy makers and scientists from 31 countries at; International Wheat Stripe Rust Symposium, Aleppo, Syria, April 2011. PP 1-23.
Birhan Abdulkadir. 2011. KARC stations distribution and website description (Un published data).
Roelfs AP., Singh RP. and Saari EE. 1992. Rust Diseases of Wheat: Concepts and Methods of Disease Management. CIMMYT, Mexico.
Peterson RF., Campbell A. and Hannah AE. 1948. A diagrammatic scale for estimating rust intensity on leaves and stems of cereals. Can. J. Res. 26: 496–500.
Large E. C. 1954. Growth stages in cereals-illustration of the Feekes scale. Plant Pathology. 3: 128-129.
AACC (American Association of Cereal Chemists). 2000. International Approved Methods of the American Association of Cereal Chemists. 10th Ed. The Association: St. Paul, MN. U.S.A. 1200. 56 Pp.
Wilcoxson R. D., Skovmand B. and Atif A. H. 1974. Evaluation of wheat cultivars ability to retard development of stem rust. Annuals of Applied Biol. 80: 275-281.
SAS. 2002. SAS/STAT guide for personal computers, version 9.0 edition. SAS Institute Inc., Cary, North Carolina, USA.
Parlevliet J. E. and van Ommeren A. 1975. Partial resistance of barely to leaf rust, Puccinia hordei. Relationship between field trials, micro plot tests, and latent period. Euphytica, 24: 293-303.
Ali S., Shah S. J. A. and Ibrahim M. 2007. Assessment of wheat breeding lines for slow yellow rusting (Puccinia striiformis West. tritici). Pakistan Journal of Biological Sciences. 10: 3440-3444.
Li Z. F., Xia X. C., He Z. H., Li X., Zhang L. J., Wang H. Y., Meng Q. F., Yang W. X., Li G. Q. and Liu D. Q. 2010. Seedling and slow rusting resistance to leaf rust in Chinese wheat cultivars. J. Plant Disease: 94: 45–53.
Safavi SA. 2012. Evaluation of slow rusting parameters in thirty-seven promising wheat lines to yellow rust. Tech J Eng Appl Sci. 2: 324–329.
Nzuve FM., Bhavani S., Tusiime G., Njau P. and Wanyera R. 2012. Evaluation of bread wheat for both seedling and adult plant resistance to stem rust. Afr J. Plant Sci. 6: 426–432.
Parlevliet J. E. 1988. Strategies for the utilization of partial resistance for the control of cereal rust. In: Simmonds NW and Rajaram S. (edds). Breeding strategies for resistance to the rusts of wheat. CIMMYT Mexico, Pp. 48-62.
Bartos P., Síp V., Chrpova J., Vacke J., Stuchlíkova E., Blažkova V., Sárová J., Hanzalová A. 2002. Achievements and prospects of wheat breeding for disease resistance. Czech J. Plant Breed. 38: 16–28.
Ibrahim S., Mohammed S., Abd-Elmageed M, Omaima A. and Ahmed F. 2014. Screening of wheat genotypes for leaf rust resistance along with grain yield. Annals of Agricultural sciences. 60 (1): 29-39.
Johnson R. 1988. Durable resistance to yellow (stripe) rust in wheat and its implications in plant breeding. In: Simmonds, N. W. and Rajaram, S. (Eds), Breeding strategies for resistance to the rusts of wheat. Mexico: CIMMYT. 63-75.
Wan A. M. and Chen X. M. 2012. Virulence, frequency, and distribution of races of Puccinia striiformis f. sp. tritici and Puccinia striiformis f. sp. hordei identified in the United States in 2008 and 2009. Plant Disease. 96: 67-74.
Singh RP., William HM., Huerta-Espino J. and Rosewarne G. 2004b. Wheat rust in Asia: meeting the challenges with old and new technologies. Proceedings of the 4th international crop science congress. September, Brisbane, Australia.
Vanderplank J. E. 1963. Plant Diseases: epidemics and control. Academic Press, New York, 113 pp.
Ali S., Shah A., Khalil IH., Raman H., Maqbool K. and Ullah W. 2009. Partial resistance to yellow rust in introduced winter wheat germplasm at the north of Pakistan. Aust J. Crop Sci. 3: 37-43.
Brown W. M. J., Hill, J. P. and Velasco V. R. 2001. Barley yellow rust in North America. Annu. Rev. Phytopathol. 39: 367-384.
Kaur J. and Bariana H. S. 2010. Inheritance of adult plant stripe rust resistance in wheat cultivars Kukri and Sunco. Journal of Plant Pathology. 92: 391-394.
Singh RP. Hodson DP., Huerta-Espino J., Jin Y., Njau P, Wanyera R, Herrera-Foessel SA. and Ward RW. 2008. Will stem rust destroy the world’s wheat crop? Adv Agron. 98: 271–309.
Ali S., Shah A. and Maqbool K. 2008. Field-based assessment of partial resistance to yellow rust in wheat germplasm. Journal of Agriculture and Rural Development. 6: 99-106.
Schafer J. F. and Roelfs A. P. 1985. Estimated relation between numbers of urediniospores of Puccinia graminis tritici and rates of occurrence of virulence. Phytopathalogy. 75: 749-750.
Draz S., Abou-Elseoud M. S., Kamara A. M., Alaa-Eldein O. A. and El-Bebany A. F. 2015. Screening of wheat genotypes for leaf rust resistance along with grain yield. Annual Agricultural Sciences. 60: 29-39.
Habte Jifar, Kebebew Assefa, Kassahun Tesfaye, Kifle Dagne and Zerihun Tadele. 2019. GenotypebyEnvironment Interaction and Stability Analysis in Grain Yield of Improved Teff (Eragrostis tef) Varieties Evaluated in Ethiopia. International Journal of Experimental Agriculture. 35 (5): 1-13.
Wu X., Chang X. and Jing R. 2012. Genetic Insight into Yield-Associated Traits of Wheat Grown in Multiple Rain-Fed Environments. PLoS ONE 7 (2): e31249.
Craigie JH. 1957. Epidemiology of Stem Rust of Cereals in Western Canada. Sci. Agri. 25: 285 - 401.
Bushnell WR. and Rowell JB. 1968. Premature death of adult rusted wheat plants in relation to carbon dioxide evolution by root system. Phytopathology 58: 651–658.
Pretorius ZA., Pakendori KW., Marais GF., Prins R. and Komen JS. 2007. Challenges for sustainable cereals rust control in South Africa. Aust. J. Agric Res. 58: 593–601.
Smedegaard-Petersen V. and Tolstrup K., 1985. The limiting effect of disease resistance on yield. Annu. Rev. Phytopathol. 23: 475–490.
Kebede Temesgen, Rouse MN., Rynearson S., Chen X., Buta BG. and Pumphrey MO. 2017. Characterization of molecular diversity and genome-wide mapping of loci associated with resistance to stripe rust and stem rust in Ethiopian bread wheat accessions. BMC Plant Biol. 17 (1): 134.
Agrios GN. 1988. Plant Pathology. 3rd edition. London, New York, Academic Press. 456 pp.
Daniel Hailegiorgis, Mebrahtom Mesfin, Tsige Genet. 2011. Genetic Divergence Analysis on some Bread Wheat Genotypes Grown in Ethiopia. Journal of Central European Agriculture. 2: 344-352.
Meseret Asmamaw. 2016. Genetic diversity and selection efficiency of Ethiopian Durum wheat (Triticum durum Desf) landrace collections. M.Sc. Thesis. Addis Ababa University, Ethiopia. Pp 1-23.
Shah SJA., Imtiaz M. and Hussain S. 2010. Phenotypic and molecular characterization of wheat for slow rusting resistance against Puccinia striiformis Westend. f. sp. tritici. J. Phytopathol. 158: 393–402.
Safavi S. A. and Afshari F. 2012. Identification of resistance to Puccinia striiformis f. sp. tritici in some elite wheat lines. Journal of Crop Protection 1: 293-302.
Science Publishing Group
1 Rockefeller Plaza,
10th and 11th Floors,
New York, NY 10020
Tel: (001)347-983-5186