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Incidence and Distribution of African Cassava Mosaic Virus in the Guinea Savannah Vegetation zone of Nigeria
Journal of Plant Sciences
Volume 9, Issue 1, February 2021, Pages: 1-8
Received: Oct. 31, 2020; Accepted: Dec. 1, 2020; Published: Jan. 12, 2021
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Authors
Tolani Adegbite Okelola, National Agricultural Seeds Council, Abuja, Nigeria; Department of Crop Protection, Faculty of Agriculture, University of Abuja, Abuja, Nigeria
Shatu Wudiri Asala, Department of Crop Protection, Faculty of Agriculture, University of Abuja, Abuja, Nigeria
Akeem Abolade Oyerinde, Department of Crop Protection, Faculty of Agriculture, University of Abuja, Abuja, Nigeria
Oladapo Oluwatobi Folarin, National Agricultural Seeds Council, Abuja, Nigeria; Department of Crop Protection, Faculty of Agriculture, University of Abuja, Abuja, Nigeria
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Abstract
African Cassava Mosaic Virus (ACMV) is one of the major diseases affecting cassava production in Sub-Saharan Africa. A survey was conducted between August and September 2019 in the Federal Capital Territory (FCT), Niger and Benue states of the Guinea savannah vegetation zone of Nigeria, where cassava is predominantly cultivated, to assess the incidence and distribution of ACMV. Leaf samples collected from this survey were subjected to molecular indexing using Polymerase Chain Reaction (PCR). Further phylogenic analysis sequencing was done to determine the diversity among the isolates obtained from the study. A total of 81 farms were visited from where 2,025 symptomatic and asymptomatic leaf samples (25 leaf samples per farm) were collected randomly in quadrants of 5m x 5m. The surveyed fields were assessed for virus disease incidence and severity by visual observation. From the samples collected, FCT had the highest incidence (62%) while Benue had the lowest (18%). FCT also recorded the highest severity while Benue had the lowest. After molecular indexing using PCR, FCT recorded the highest incidence to ACMV (22.22%), while no ACMV incidence was recorded in Benue state. The sequencing and phylogenic analysis reveals that there is a high diversity among the isolates obtained in the areas surveyed. This study indicates the presence of ACMV in the Guinea Savannah vegetation zone of Nigeria and has confirmed that cassava Mosaic Disease could be a threat to cassava production in the zone, therefore, the need for quick diagnosis.
Keywords
Virus, Survey, Cassava, Cassava Mosaic Disease, Polymerase Chain Reaction (PCR)
To cite this article
Tolani Adegbite Okelola, Shatu Wudiri Asala, Akeem Abolade Oyerinde, Oladapo Oluwatobi Folarin, Incidence and Distribution of African Cassava Mosaic Virus in the Guinea Savannah Vegetation zone of Nigeria, Journal of Plant Sciences. Vol. 9, No. 1, 2021, pp. 1-8. doi: 10.11648/j.jps.20210901.11
Copyright
Copyright © 2021 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
References
[1]
Jisha, S., Padmaja G. and Sajeev, M. S. (2010). Nutritional and textural studies on dietary fiber-enriched muffins and biscuits from cassava-based composite flours. Journal of Food Quality 33: 79–99.
[2]
Food and Agriculture Organization (2014). Crop Production data 2013, FAOSTAT. Rome, Italy. Available at: http://www.fao.org.
[3]
Afoakwa, E. O., Asiedu, C., Budu, A. S., Chiwona-Karltun, L., Nyirendah, D. B. (2012). Chemical composition and cyanogenic potential of traditional and high yielding CMD resistant cassava (Manihot esculenta Crantz) varieties. International Food Research Journal 19 (1): 175-181.
[4]
Fauquet, C. M., Maxwell, D. P., Gronenborn, B. & Stanley, J. (2008). Revised proposal for naming of geminiviruses. Archives of Virology 145/8, 1744-1761.
[5]
Fauquet, C. M. & Stanley, J. (2003). Geminivirus classification and nomenclanture: progress and problems. Annals of Applied Biology 142, 165-189.
[6]
Thresh J. M, Cooter RJ (2005). Strategies for controlling cassava mosaic virus disease in Africa. Plant Pathol. 54:587-614. infections with African cassava mosaic virus in Nigeria. Plant Dis. 87: 229-232.
[7]
Ogbe F. O, Atiri GI, Dixon A. G. O, Thottappilly G (2003). Cassava mosaic disease and its causal agents: the Nigerian situation In: Proceedings of the First International Conference on Plant Virology in Sub-Saharan Africa (4–8 June 2001, Ibadan, Nigeria), IITA, Ibadan, Nigeria. pp. 411-422.
[8]
Alabi, O. J., Kumar, P. L., and Naidu, R. A. (2011). Cassava mosaic disease: A curse to food security in Sub-Saharan Africa. Online. APSnet Features. doi:10.1094/APSnetFeature-2011-0701.
[9]
Ogbe F. O, Dixon A. G. O, Hughes J, Alabi O. J, Okechukwu R (2006). Status of cassava begomoviruses and their new natural hosts in Nigeria. Plant Dis. 90: 548-553.
[10]
Alabi O. J, Kumar P. L, Rayapati A. N. (2008). Multiplex PCR for the detection of African cassava mosaic virus and East African cassava mosaic Cameroon virus in cassava. J. Virol. Methods 154: 111-120.
[11]
Eni A. O., Fasasi D. K. (2013). Molecular detection of two cassava Begomoviruses in some parts of Southern Nigeria. African Journal of Agricultural Research. Vol. 8 (16), pp. 1350 -1353.
[12]
Mulenga, R. M., Legg, J. P., Ndunguru, J., Chikoti, P. C., Miano, D. W., Mutitu, W. E., and Alabi, O. J. (2016). Survey, molecular detection, and char-acterization of geminiviruses associated with cassava mosaic disease in Zambia. Plant Dis. 100: 1379-1387.
[13]
Asala, Shatu Wudiri & Alegbejo, Matthew & Kashina, B. & Banwo, Olalekan & Robert, Asiedu & Kumar, Lava. (2012). Distribution and incidence of viruses infecting yam (Dioscorea spp.) in Nigeria. Glob. J. Biosci. Biotechnol. Lett.. 1. 163-167.
[14]
Odedara, O. O., Hughes, J. Ode bode, A. C., Odu, B. O. (2008). Multiple Virus infection of lablab in Nigeria. Journal of General Pathology. 74: 322-325.
[15]
Eni A. O., Hughes J. D and Rey M. E. C (2008). Survey of the incidence and distribution of five viruses infecting yam in major yam producing zones of Nigeria. Annals of applied Biology 153: 223-232.
[16]
Abarshi, M. M., Mohammed, I. U., Wasswa, P., Hillocks, R. J., Holt, J., Legg, J. P. Seal, S. E.; Maruthi, M. N. (2010). Optimization of diagnostic RT-PCR protocols and sampling procedures for the reliable and cost-effective detection of Cassava brown streak virus. Journal of Virological Methods, 163, 353–359.
[17]
Asare, P. A., Galyuon, I. K. A., Asare-Bediako, E., Sarfo, J. K., Tetteh, J. P. (2014). Phenotypic and molecular screening of cassava (Manihot esculentum Crantz) genotypes for resistance to cassava mosaic disease. Journal of General and Molecular Virology, 6 (2), 6-18.
[18]
Alabi, O. J., Mulenga, R. M., Legg, J. P. (2015). Cassava mosaic. In: Virus diseases of tropical and subtropical crops, [ed. by Tennant, P., Fermin, G.]. Wallingford, UK: CAB International. 56-72.
[19]
Eni, A. O., Efekemo, O. P., Soluade, M. G., Popoola, S. I., & Atayero, A. A. (2018). Incidence of cassava mosaic disease and associated whitefly vectors in South West and North Central Nigeria: Data exploration. Data in Brief, 19, 370–392. https://doi.org/10.1016/J.DIB.2018.05.016.
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