Effect of Growth Stage and Solvent Extract on the Antioxidant Potential of Olive Leaves
Journal of Plant Sciences
Volume 3, Issue 3-1, June 2015, Pages: 1-7
Received: Feb. 11, 2015; Accepted: Feb. 11, 2015; Published: Apr. 23, 2015
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Authors
Faten Brahmi, Laboratory of Biochemistry,UR03/ES-08 ‘‘Human Nutrition and Metabolic Disorder”, Faculty of Medicine, University of Monastir, Monastir ,Tunisia
Beligh Mechri, Laboratory of Biochemistry,UR03/ES-08 ‘‘Human Nutrition and Metabolic Disorder”, Faculty of Medicine, University of Monastir, Monastir ,Tunisia
Madiha Dhibi, Laboratory of Biochemistry,UR03/ES-08 ‘‘Human Nutrition and Metabolic Disorder”, Faculty of Medicine, University of Monastir, Monastir ,Tunisia
Mohamed Hammami, Laboratory of Biochemistry,UR03/ES-08 ‘‘Human Nutrition and Metabolic Disorder”, Faculty of Medicine, University of Monastir, Monastir ,Tunisia
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Abstract
The effect of growth stage on the physicochemical composition and antioxydant activities of olive leaves extracted with solvents of increasing polarity was investigated at two different stages. The total phenolic, total o-diphenols and total flavonoid were measured. Therefore, the levels of chlorophyll and carotenoids were also measured. The content of phenols varied significantly with two different growth stages October and February. The lowest level of phenols and o-diphenols was detected in the first vegetative stage whereas the highest content was enregistered during the second vegetative stage. The results showed that methanol extract contains the maximum phenolic content. Furthermore, the differences in total flavonoid contents of leaves between two different stages and between solvent extract were highly significant. Thus, the AA exhibited asignificant (p<0.05) increase during leaves developpement. In fact, methanol and ethyl acetate extract was a considerably more effective on DPPH radical scavenger than other solvents extracts.
Keywords
Olea europaea L., Growth Stage, Antioxidant Activity, Chlorophylls, Phytochemical Composition
To cite this article
Faten Brahmi, Beligh Mechri, Madiha Dhibi, Mohamed Hammami, Effect of Growth Stage and Solvent Extract on the Antioxidant Potential of Olive Leaves, Journal of Plant Sciences. Special Issue: Valorization of Leaves, Fruits and Stems of Aromatic Plants and Studies of Their Vegetative Cycle ; Extraction, Purification and Biological Activities. Vol. 3, No. 3-1, 2015, pp. 1-7. doi: 10.11648/j.jps.s.2015030301.11
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