Molecular Characterization of Indian Potato (Solanum tuberosum L.) Varieties for Cold-Induced Sweetening Using SSR Markers

Galani Yamdeu Joseph Hubert; Pooja H. Gupta; Nilesh J. Patel; Avadh K. Shah; Rajeshkumar R. Acharya; Jayantkumar G. Talati

doi:doi:10.11648/j.jps.20150304.14

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Molecular Characterization of Indian Potato (Solanum tuberosum L.) Varieties for Cold-Induced Sweetening Using SSR Markers

Galani Yamdeu Joseph Hubert, Pooja H. Gupta, Nilesh J. Patel, Avadh K. Shah, Rajeshkumar R. Acharya, Jayantkumar G. Talati

Received: 27 June 2015 Accepted: 4 July 2015 Published: 14 July 2015

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Abstract

Cold-induced sweetening developed during storage of potatoes (Solanum tuberosum L.) at low temperature is a crucial factor influencing the processing quality of potato tubers and remains one of the principal concerns of potato processing industry. Developing CIS-resistant genotypes is the most effective method to cope with this stress. In this study, the genetic diversity of 11 Indian potato varieties with different reactions to CIS was assessed using 10 SSR primers. The primers detected a total of 42 alleles arranged in 44 different configurations, among which 37 alleles (88%) were polymorphic. The polymorphic information content (PIC) value of the SSR locus ranged from 0.473 to 0.787 thus indicating a high utility of these markers for study of genetic diversity in potato. A number of polymorphic fragments appeared to be specific to a given sugar-forming group. Primer Sti007 generated one fragment Sti007131bp present only in all the high sugar-forming varieties. The dendrogram derived from Dice’s similarity coefficients among the 11 varieties could partially but efficiently differentiate close parents and sugar-forming groups among the varieties. These findings demonstrate the effectiveness of SSR markers to assess the genetic variation among potato cultivars in order to develop molecular markers associated with CIS to improve potato breeding programs.

DOI	10.11648/j.jps.20150304.14
Published in	Journal of Plant Sciences (Volume 3, Issue 4, August 2015)
Page(s)	191-196
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Cold-Induced Sweetening, Potato, Solanum tuberosum, SSR, Genetic Diversity

References

[1]	Marwaha RS, Pandey SK, Kumar D, Singh SV, Kumar P (2010) Potato processing scenario in India: Industrial constraints future projections challenges ahead and remedies – A review. Journal of Food Science Technology 47(2):137–156.
[2]	Burton WG (1969) The sugar balance in some british potato varieties during storage II The effects of tuber age previous storage temperature and intermittent refrigeration upon low-temperature sweetening. European Potato Journal 12: 81–95
[3]	Chuda Y, Ono H, Yada H, Takada AO, Endo CM, Mori M (2003) Effects of physiological changes in potato tubers (Solanum tuberosum L) after low temperature storage on the level of acrylamide formed in potato chips. Biological Science Biotechnology and Biochemistry 67(5): 1188-1190.
[4]	Hogervorst JG, Schouten LJ, KoningsEJ, Goldbohm RA, van den Brandt PA (2007) A prospective study of dietary acrylamide intake and the risk of endometrial ovarian and breast cancer. Cancer Epidemiology and Biomarkers Preview 16(11): 2304-2313.
[5]	MckenzieMJ, Chen RKY, Harris JC, Ashworth MJ, Brummell DA (2013) Post-translational regulation of acid invertase activity by vacuolar invertase inhibitor affects resistance to cold-induced sweetening of potato tubers. Plant Cell and Environment 36: 176–185.
[6]	Anonymous (2010) Central Potato Research Institute (CPRI) Newsletter Number 43, December 2010: 4-5
[7]	Chen X, Song B, Liu J, Yang J, He T, Lin Y, Zhang H, Xie C (2012) Modulation of gene expression in cold-induced sweetening resistant potato species Solanum berthaultii exposed to low temperature. Molecular Genetics and Genomics 287: 411–421
[8]	Yildirim A, Kandemir N, ÖAte S, Güleç TL (2009) Transferability of microsatellite markers among cool season cereals. Biotechnology and Biotechnological Equipment 23(3): 1299-1302.
[9]	Provan J, Powell W, Waugh R (1996) Microsatellite analysis of relationships within cultivated potato (Solanum tuberosum). Theoretical and Applied Genetics 92: 1078-1084.
[10]	Milbourne D, Meyer R, Collins AJ, Ramsay LD, Gebhardt C, Waugh R (1998) Isolation characterisation and mapping of simple sequence repeat loci in potato. Molecular Genetics and Genomics 259: 233-245.
[11]	Ghislain M, Spooner DM, Rodríguez F, Villamón F, Núňez J, Vasquez C, Waugh R, Bonierbale M (2004) Selection of highly informative and user-friendly microsatellites (SSRs) for genotyping of cultivated potato. Theoretical and Applied Genetics 108: 881-890.
[12]	Feingold S, Lloyd J, Norero N, Bonierbale M, Lorenzen J (2005) Mapping and characterization of new EST-derived microsatellites for potato (Solanum tuberosum L). Theoretical Applied Genetics 111: 456-466.
[13]	Barandalla L, Ruiz de GalarretaJI, Rios D, Ritter E (2006) Molecular analysis of local potato cultivars from Tenerife Island using microsatellite markers. Euphytica 152: 283–291.
[14]	Kandemir N, Yılmaz G, Karan YB, Borazan D (2010) Development of a simple sequence repeat (SSR) marker set to fingerprint local and modern potato varieties grown in central Anatolian Plateau in Turkey. African Journal of Biotechnology 9(34): 5516-5522
[15]	Rocha EA, Paiva LV, CarvalhoHH, Guimarães CT (2010) Molecular characterization and genetic diversity of potato cultivars using SSR and RAPD markers. Crop Breeding and Applied Biotechnology 10: 204-210
[16]	Favoretto P, VeaseyEA, MeloPCT (2011) Molecular characterization of potato cultivars using SSR markers. HorticulturaBrasileira 29: 542-547.
[17]	GalarretaJIR, Barandalla L, Rios DJ, Lopez R, Ritter E (2011) Genetic relationships among local potato cultivars from Spain using SSR markers. Genetic Resources and Crop Evolution 58: 383-395.
[18]	Chimote VP, Pattanayak D, Naik PS (2007) Molecular and morphological divergence studies in Indian potato varieties. Indian Journal of Biotechnology 6: 216-223.
[19]	Tiwari JK, Singh BP, Gopal J, Poonam, Patil VU (2013) Molecular characterization of the Indian Andigena potato core collection using microsatellite markers. African Journal of Biotechnology 12(10): 1025-1033.
[20]	Datir S S (2011) Cold-induced sweetening in potato (Solanum tuberosum L): genetic analysis of the apoplastic invertase inhibitor gene. Dissertation, Lincoln University New Zealand. 231
[21]	Galani YJH (2014) Biochemical and Molecular Characterization of Cold-Induced Sweetening in Potato (Solanum tuberosum L) Varieties during Storage. Dissertation. Anand Agricultural University, India. 310
[22]	El-Komy MH, Saleh AA, MolanYY (2012) Molecular characterization of early blight disease resistant and susceptible potato cultivars using random amplified polymorphic DNA (RAPD) and simple sequence repeats (SSR) markers. African Journal of Biotechnology 11(1): 37-45.
[23]	Sambrook J, Russell DW (2001) Molecular Cloning: A laboratory manual. (Third Edition), Cold Spring Harbor, Laboratory Press Cold Spring Harbor, New York, USA, 999.
[24]	Moisan-Thiery M, Marhadour S, Kerlan MC, Dessenne N, Perramant M, Gokelaere T, Le Hingrat Y (2005) Potato cultivar identification using simple sequence repeats markers (SSR). Potato Research 48: 191-200.
[25]	Nei M (1973) Analysis of gene diversity in subdivided populations. Proceedings of National Academy of Science USA 70: 3321-3323.
[26]	Rohlf F (2006) NTSYSpc: Numerical taxonomy system (ver 22). Exeter Publishing Ltd, Setauket, NY, USA.

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APA Style

Galani Yamdeu Joseph Hubert, Pooja H. Gupta, Nilesh J. Patel, Avadh K. Shah, Rajeshkumar R. Acharya, et al. (2015). Molecular Characterization of Indian Potato (Solanum tuberosum L.) Varieties for Cold-Induced Sweetening Using SSR Markers. Journal of Plant Sciences, 3(4), 191-196. https://doi.org/10.11648/j.jps.20150304.14

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ACS Style

Galani Yamdeu Joseph Hubert; Pooja H. Gupta; Nilesh J. Patel; Avadh K. Shah; Rajeshkumar R. Acharya, et al. Molecular Characterization of Indian Potato (Solanum tuberosum L.) Varieties for Cold-Induced Sweetening Using SSR Markers. J. Plant Sci. 2015, 3(4), 191-196. doi: 10.11648/j.jps.20150304.14

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AMA Style

Galani Yamdeu Joseph Hubert, Pooja H. Gupta, Nilesh J. Patel, Avadh K. Shah, Rajeshkumar R. Acharya, et al. Molecular Characterization of Indian Potato (Solanum tuberosum L.) Varieties for Cold-Induced Sweetening Using SSR Markers. J Plant Sci. 2015;3(4):191-196. doi: 10.11648/j.jps.20150304.14

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@article{10.11648/j.jps.20150304.14,
  author = {Galani Yamdeu Joseph Hubert and Pooja H. Gupta and Nilesh J. Patel and Avadh K. Shah and Rajeshkumar R. Acharya and Jayantkumar G. Talati},
  title = {Molecular Characterization of Indian Potato (Solanum tuberosum L.) Varieties for Cold-Induced Sweetening Using SSR Markers},
  journal = {Journal of Plant Sciences},
  volume = {3},
  number = {4},
  pages = {191-196},
  doi = {10.11648/j.jps.20150304.14},
  url = {https://doi.org/10.11648/j.jps.20150304.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jps.20150304.14},
  abstract = {Cold-induced sweetening developed during storage of potatoes (Solanum tuberosum L.) at low temperature is a crucial factor influencing the processing quality of potato tubers and remains one of the principal concerns of potato processing industry. Developing CIS-resistant genotypes is the most effective method to cope with this stress. In this study, the genetic diversity of 11 Indian potato varieties with different reactions to CIS was assessed using 10 SSR primers. The primers detected a total of 42 alleles arranged in 44 different configurations, among which 37 alleles (88%) were polymorphic. The polymorphic information content (PIC) value of the SSR locus ranged from 0.473 to 0.787 thus indicating a high utility of these markers for study of genetic diversity in potato. A number of polymorphic fragments appeared to be specific to a given sugar-forming group. Primer Sti007 generated one fragment Sti007131bp present only in all the high sugar-forming varieties. The dendrogram derived from Dice’s similarity coefficients among the 11 varieties could partially but efficiently differentiate close parents and sugar-forming groups among the varieties. These findings demonstrate the effectiveness of SSR markers to assess the genetic variation among potato cultivars in order to develop molecular markers associated with CIS to improve potato breeding programs.},
 year = {2015}
}

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TY - JOUR
T1 - Molecular Characterization of Indian Potato (Solanum tuberosum L.) Varieties for Cold-Induced Sweetening Using SSR Markers
AU - Galani Yamdeu Joseph Hubert
AU - Pooja H. Gupta
AU - Nilesh J. Patel
AU - Avadh K. Shah
AU - Rajeshkumar R. Acharya
AU - Jayantkumar G. Talati
Y1 - 2015/07/14
PY - 2015
N1 - https://doi.org/10.11648/j.jps.20150304.14
DO - 10.11648/j.jps.20150304.14
T2 - Journal of Plant Sciences
JF - Journal of Plant Sciences
JO - Journal of Plant Sciences
SP - 191
EP - 196
PB - Science Publishing Group
SN - 2331-0731
UR - https://doi.org/10.11648/j.jps.20150304.14
AB - Cold-induced sweetening developed during storage of potatoes (Solanum tuberosum L.) at low temperature is a crucial factor influencing the processing quality of potato tubers and remains one of the principal concerns of potato processing industry. Developing CIS-resistant genotypes is the most effective method to cope with this stress. In this study, the genetic diversity of 11 Indian potato varieties with different reactions to CIS was assessed using 10 SSR primers. The primers detected a total of 42 alleles arranged in 44 different configurations, among which 37 alleles (88%) were polymorphic. The polymorphic information content (PIC) value of the SSR locus ranged from 0.473 to 0.787 thus indicating a high utility of these markers for study of genetic diversity in potato. A number of polymorphic fragments appeared to be specific to a given sugar-forming group. Primer Sti007 generated one fragment Sti007131bp present only in all the high sugar-forming varieties. The dendrogram derived from Dice’s similarity coefficients among the 11 varieties could partially but efficiently differentiate close parents and sugar-forming groups among the varieties. These findings demonstrate the effectiveness of SSR markers to assess the genetic variation among potato cultivars in order to develop molecular markers associated with CIS to improve potato breeding programs.
VL - 3
IS - 4
ER -

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Author Information

Galani Yamdeu Joseph Hubert

Department of Agriculture and Veterinary Medicine, Université des Montagnes, Bangangté, Cameroon
Pooja H. Gupta

Department of Biochemistry, B.A. College of Agriculture, Anand Agricultural University, Gujarat, India
Nilesh J. Patel

Department of Biochemistry, B.A. College of Agriculture, Anand Agricultural University, Gujarat, India
Avadh K. Shah

Department of Biochemistry, B.A. College of Agriculture, Anand Agricultural University, Gujarat, India
Rajeshkumar R. Acharya

Main Vegetable Research Station, Anand Agricultural University, Gujarat, India
Jayantkumar G. Talati

Department of Biochemistry, B.A. College of Agriculture, Anand Agricultural University, Gujarat, India

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APA Style

Galani Yamdeu Joseph Hubert, Pooja H. Gupta, Nilesh J. Patel, Avadh K. Shah, Rajeshkumar R. Acharya, et al. (2015). Molecular Characterization of Indian Potato (Solanum tuberosum L.) Varieties for Cold-Induced Sweetening Using SSR Markers. Journal of Plant Sciences, 3(4), 191-196. https://doi.org/10.11648/j.jps.20150304.14

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ACS Style

Galani Yamdeu Joseph Hubert; Pooja H. Gupta; Nilesh J. Patel; Avadh K. Shah; Rajeshkumar R. Acharya, et al. Molecular Characterization of Indian Potato (Solanum tuberosum L.) Varieties for Cold-Induced Sweetening Using SSR Markers. J. Plant Sci. 2015, 3(4), 191-196. doi: 10.11648/j.jps.20150304.14

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AMA Style

Galani Yamdeu Joseph Hubert, Pooja H. Gupta, Nilesh J. Patel, Avadh K. Shah, Rajeshkumar R. Acharya, et al. Molecular Characterization of Indian Potato (Solanum tuberosum L.) Varieties for Cold-Induced Sweetening Using SSR Markers. J Plant Sci. 2015;3(4):191-196. doi: 10.11648/j.jps.20150304.14

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@article{10.11648/j.jps.20150304.14,
  author = {Galani Yamdeu Joseph Hubert and Pooja H. Gupta and Nilesh J. Patel and Avadh K. Shah and Rajeshkumar R. Acharya and Jayantkumar G. Talati},
  title = {Molecular Characterization of Indian Potato (Solanum tuberosum L.) Varieties for Cold-Induced Sweetening Using SSR Markers},
  journal = {Journal of Plant Sciences},
  volume = {3},
  number = {4},
  pages = {191-196},
  doi = {10.11648/j.jps.20150304.14},
  url = {https://doi.org/10.11648/j.jps.20150304.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jps.20150304.14},
  abstract = {Cold-induced sweetening developed during storage of potatoes (Solanum tuberosum L.) at low temperature is a crucial factor influencing the processing quality of potato tubers and remains one of the principal concerns of potato processing industry. Developing CIS-resistant genotypes is the most effective method to cope with this stress. In this study, the genetic diversity of 11 Indian potato varieties with different reactions to CIS was assessed using 10 SSR primers. The primers detected a total of 42 alleles arranged in 44 different configurations, among which 37 alleles (88%) were polymorphic. The polymorphic information content (PIC) value of the SSR locus ranged from 0.473 to 0.787 thus indicating a high utility of these markers for study of genetic diversity in potato. A number of polymorphic fragments appeared to be specific to a given sugar-forming group. Primer Sti007 generated one fragment Sti007131bp present only in all the high sugar-forming varieties. The dendrogram derived from Dice’s similarity coefficients among the 11 varieties could partially but efficiently differentiate close parents and sugar-forming groups among the varieties. These findings demonstrate the effectiveness of SSR markers to assess the genetic variation among potato cultivars in order to develop molecular markers associated with CIS to improve potato breeding programs.},
 year = {2015}
}

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