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Looking Inside Non-coding Chloroplast Regions of Calophyllum brasiliense (Calophyllaceae) to Understand Its Southernmost Population Distribution

Received: 06 October 2015    Accepted: 23 October 2015    Published: 19 November 2015
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Abstract

In recent years the growing interest in the conservation of Paraná River’s riparian forest led to the discovery of botanical novelties for Argentina. Populations of Calophyllum brasiliense Camb. (Calophyllaceae), a typically flooded lowlands species, were identified in the remaining hygrophile forest of northeast Argentina and southeast Paraguay. Deforestation and flooding, due to the construction of dams, have caused these populations to suffer intensive fragmentation. The aim of this work was to infer phylogeographic relationships among five populations of C. brasiliense, three from Argentina and two from Paraguay, which represent the southernmost points of species’ distribution. We also compared them with samples of a C. brasiliense population from Mexico, the northernmost edge of the species distribution. The chloroplast intergenic spacers petG-trnP, psbJ-petA and the trnL-UAA chloroplast intron were amplified from leaves’ DNA. A total of 2234 bp were characterized once the three regions were analyzed. The three chloroplast regions showed nucleotide differences, represented by InDels, inversions and a few SNPs; however, only the trnL intron was selected for further phylogeographic analysis due to the amount of the information obtained for all populations. Based on trnL intron, it was possible to estimate nucleotide and haplotype diversity (π = 0.00237 and Hd = 0.29600, respectively). Three haplotypes were identified, which allowed Argentinean, Paraguayan and Mexican populations to be differentiated. Based on the three haplotypes found, we discuss and propose a model for a C. brasiliense’ geographic dispersion and historical colonization routes, including an alternative new one to the well-known of the Paraná River.

DOI 10.11648/j.jps.20150306.14
Published in Journal of Plant Sciences (Volume 3, Issue 6, December 2015)
Page(s) 310-319
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

C. brasiliense, cpDNA, petG-trnP, psbJ-petA, trnL Intron

References
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Author Information
  • Laboratorio GIGA, Instituto de Biología Subtropical, nodo Posadas, Universidad Nacional de Misiones, Consejo Nacional de Investigaciones Científicas y Técnicas. Posadas, Argentina

  • Laboratorio GIGA, Instituto de Biología Subtropical, nodo Posadas, Universidad Nacional de Misiones, Consejo Nacional de Investigaciones Científicas y Técnicas. Posadas, Argentina

  • Cátedra de Biología Vegetal. Facultad de Ciencias Exactas Químicas y Naturales, Universidad Nacional de Misiones. Posadas, Argentina

  • Cátedra de Sistemática Teórica. Facultad de Ciencias Exactas Químicas y Naturales, Universidad Nacional de Misiones. Posadas, Argentina

  • Cátedra de Introducción a las Ciencias de la Tierra. Facultad de Ciencias Exactas Químicas y Naturales, Universidad Nacional de Misiones, Posadas, Argentina

  • Laboratorio de Sistemática y Biología Evolutiva. Facultad de Ciencias Naturales y Museo, Universidad Nacional de La Plata. La Plata, Argentina

  • Laboratorio GIGA, Instituto de Biología Subtropical, nodo Posadas, Universidad Nacional de Misiones, Consejo Nacional de Investigaciones Científicas y Técnicas. Posadas, Argentina

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    Cecilia Beatriz Percuoco, Liliana Noelia Talavera Stéfani, Manuela Edith Rodríguez, Naiké Lucía González, Juan Fernando Crivello, et al. (2015). Looking Inside Non-coding Chloroplast Regions of Calophyllum brasiliense (Calophyllaceae) to Understand Its Southernmost Population Distribution. Journal of Plant Sciences, 3(6), 310-319. https://doi.org/10.11648/j.jps.20150306.14

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    Cecilia Beatriz Percuoco; Liliana Noelia Talavera Stéfani; Manuela Edith Rodríguez; Naiké Lucía González; Juan Fernando Crivello, et al. Looking Inside Non-coding Chloroplast Regions of Calophyllum brasiliense (Calophyllaceae) to Understand Its Southernmost Population Distribution. J. Plant Sci. 2015, 3(6), 310-319. doi: 10.11648/j.jps.20150306.14

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    Cecilia Beatriz Percuoco, Liliana Noelia Talavera Stéfani, Manuela Edith Rodríguez, Naiké Lucía González, Juan Fernando Crivello, et al. Looking Inside Non-coding Chloroplast Regions of Calophyllum brasiliense (Calophyllaceae) to Understand Its Southernmost Population Distribution. J Plant Sci. 2015;3(6):310-319. doi: 10.11648/j.jps.20150306.14

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  • @article{10.11648/j.jps.20150306.14,
      author = {Cecilia Beatriz Percuoco and Liliana Noelia Talavera Stéfani and Manuela Edith Rodríguez and Naiké Lucía González and Juan Fernando Crivello and Jorge Víctor Crisci and Carina Francisca Argüelles},
      title = {Looking Inside Non-coding Chloroplast Regions of Calophyllum brasiliense (Calophyllaceae) to Understand Its Southernmost Population Distribution},
      journal = {Journal of Plant Sciences},
      volume = {3},
      number = {6},
      pages = {310-319},
      doi = {10.11648/j.jps.20150306.14},
      url = {https://doi.org/10.11648/j.jps.20150306.14},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.jps.20150306.14},
      abstract = {In recent years the growing interest in the conservation of Paraná River’s riparian forest led to the discovery of botanical novelties for Argentina. Populations of Calophyllum brasiliense Camb. (Calophyllaceae), a typically flooded lowlands species, were identified in the remaining hygrophile forest of northeast Argentina and southeast Paraguay. Deforestation and flooding, due to the construction of dams, have caused these populations to suffer intensive fragmentation. The aim of this work was to infer phylogeographic relationships among five populations of C. brasiliense, three from Argentina and two from Paraguay, which represent the southernmost points of species’ distribution. We also compared them with samples of a C. brasiliense population from Mexico, the northernmost edge of the species distribution. The chloroplast intergenic spacers petG-trnP, psbJ-petA and the trnL-UAA chloroplast intron were amplified from leaves’ DNA. A total of 2234 bp were characterized once the three regions were analyzed. The three chloroplast regions showed nucleotide differences, represented by InDels, inversions and a few SNPs; however, only the trnL intron was selected for further phylogeographic analysis due to the amount of the information obtained for all populations. Based on trnL intron, it was possible to estimate nucleotide and haplotype diversity (π = 0.00237 and Hd = 0.29600, respectively). Three haplotypes were identified, which allowed Argentinean, Paraguayan and Mexican populations to be differentiated. Based on the three haplotypes found, we discuss and propose a model for a C. brasiliense’ geographic dispersion and historical colonization routes, including an alternative new one to the well-known of the Paraná River.},
     year = {2015}
    }
    

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  • TY  - JOUR
    T1  - Looking Inside Non-coding Chloroplast Regions of Calophyllum brasiliense (Calophyllaceae) to Understand Its Southernmost Population Distribution
    AU  - Cecilia Beatriz Percuoco
    AU  - Liliana Noelia Talavera Stéfani
    AU  - Manuela Edith Rodríguez
    AU  - Naiké Lucía González
    AU  - Juan Fernando Crivello
    AU  - Jorge Víctor Crisci
    AU  - Carina Francisca Argüelles
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    DO  - 10.11648/j.jps.20150306.14
    T2  - Journal of Plant Sciences
    JF  - Journal of Plant Sciences
    JO  - Journal of Plant Sciences
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    EP  - 319
    PB  - Science Publishing Group
    SN  - 2331-0731
    UR  - https://doi.org/10.11648/j.jps.20150306.14
    AB  - In recent years the growing interest in the conservation of Paraná River’s riparian forest led to the discovery of botanical novelties for Argentina. Populations of Calophyllum brasiliense Camb. (Calophyllaceae), a typically flooded lowlands species, were identified in the remaining hygrophile forest of northeast Argentina and southeast Paraguay. Deforestation and flooding, due to the construction of dams, have caused these populations to suffer intensive fragmentation. The aim of this work was to infer phylogeographic relationships among five populations of C. brasiliense, three from Argentina and two from Paraguay, which represent the southernmost points of species’ distribution. We also compared them with samples of a C. brasiliense population from Mexico, the northernmost edge of the species distribution. The chloroplast intergenic spacers petG-trnP, psbJ-petA and the trnL-UAA chloroplast intron were amplified from leaves’ DNA. A total of 2234 bp were characterized once the three regions were analyzed. The three chloroplast regions showed nucleotide differences, represented by InDels, inversions and a few SNPs; however, only the trnL intron was selected for further phylogeographic analysis due to the amount of the information obtained for all populations. Based on trnL intron, it was possible to estimate nucleotide and haplotype diversity (π = 0.00237 and Hd = 0.29600, respectively). Three haplotypes were identified, which allowed Argentinean, Paraguayan and Mexican populations to be differentiated. Based on the three haplotypes found, we discuss and propose a model for a C. brasiliense’ geographic dispersion and historical colonization routes, including an alternative new one to the well-known of the Paraná River.
    VL  - 3
    IS  - 6
    ER  - 

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