Journal of Plant Sciences

| Peer-Reviewed |

The Use of Encapsulation-Dehydration Technique for Short-Term Preservation of Endangered Sweet Potato Landraces (Ipomoea batatas Lam) from Benin

Received: 25 June 2018    Accepted: 11 July 2018    Published: 06 August 2018
Views:       Downloads:

Share This Article

Abstract

Ex situ conservation of genetic resources remains a challenge for preservation of vegetatively propagated species such as sweet potato. The objective of this study was to establish the protocol based on the encapsulation-dehydration technique for short term preservation of endangered sweet potato landraces produced in Benin. Thus, the evaporative dehydration duration on silicagel was previously determinated on the empty beads which were made using alginate sodium (3%) and calcium chloride (1.32M). Then, the young shoots of two sweet potato landraces (Koïdokpon and Dokoui carotte) growing in the screen house were cutted aseptically and desinfected with 10% sodium hypochlorite. The apices were excised on stereoscope and were encapsulated prior their dehydration on silicagel during 5 and 6h. The encapsulated apices were finally conserved in eppendorf tubes at 2°C in batches for 15 days and 90 days. The encapsulated apices were cultured in MS medium supplemented with 0.15 mg/l BAP, 0.2 mg/l NAA, 0.08 mg/l GA3 and 80 mg/l adenine sulfate. The survival and the regeneration rates were then evaluated. At our experimentation condition, the dehydration duration which allowed around 20% water content of the beads was 6h. For the encapsulated apices, the highest survival rates (59.26% and 37.04%) and the highest regeneration rate (37.04% and 11.11%) were recorded respectivelly with the landraces ''Koïdokpon'' and ''Dokoui carotte'' when the apices were dehydrated by 6h and stored for 15 days. The regeneration rates decreased according to the stored duration. Significant difference was noted on the regeneration of apices for the landraces tested. This method can be used to preserve the endangered sweet potato landraces and other species during at least three months without subcultures. It also reduce the cost of conservation in terms of consumables and permit better genotype stability during the storage.

DOI 10.11648/j.jps.20180603.13
Published in Journal of Plant Sciences (Volume 6, Issue 3, June 2018)
Page(s) 93-100
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Sweet Potato, Ex situ Preservation, Artificial Seeds, Shoot Apices, Benin

References
[1] Doussoh, A. M., Dangou, J. S., Houedjissin, S. S., Assogba, A. K. and Ahanhanzo, C. (2016) Analyse des connaissances endogènes et des déterminants de la production de la patate douce [Ipomoea batatas (L.)], une culture à haute valeur socioculturelle et économique au Bénin. International Journal of Biological and Chemical Sciences 10(6): 2596-2616.
[2] Sanoussi, A., Adjatin, A., Dansi, A., Adebowale, A., Sanni, L. and Sanni, A. (2016) Mineral Composition of Ten Elites Sweet Potato (Ipomoea Batatas [L.] Lam.) Landraces of Benin. International Journal of Current Microbiology and Applied Sciences 5(1): 103-115.
[3] Doussoh, A., Dangou-Sossou, J., Houédjissin, S., Cacaï, G., Assogba, A. and Ahanhanzo, C. (2017) Influence of mercuric chloride on survival and suitability for in vitro regeneration of three sweet potato landraces (Ipomoea batatas L.) produced in Benin. International Journal of Current Research in Biosciences and Plant Bioogy. 4(1): 56-64.
[4] González-Arnao, M. T., Dolce, N., González-Benito, M. E., Martínez, C. R. C. and Cruz-Cruz, C. A. (2017). Approaches for In Vitro Conservation of Woody Plants Germplasm. In Biodiversity and Conservation of Woody Plants. Springer 355-419.
[5] Gonzalez-Arnao, M. T. and Engelmann, F. (2006) Cryopreservation of plant germplasm using the encapsulation-dehydration technique: review and case study on sugarcane. CryoLetters 27(3): 155-168.
[6] Agbidinoukoun, A., Ahanhanzo, C., Adoukonou-Sagbadja, H., Adjassa, M., Djikpo-Tchibozo, M. A. and Agbangla, C. (2013) Impact of osmotic dehydration on the encapsulated apices survival of two yams (Dioscorea spp.) genotypes from Benin. Journal of Applied Biosciences 65: 4999-5007
[7] Alam, I., Sharmin, S. A., Naher, K., Alam, J., Anisuzzaman, M. and Alam, M. F. (2010) Effect of Growth Regulators on Meristem Culture and Plantlet Establishment in Sweet Potato ['Ipomoea Batatas'(L.) Lam.]. Plant Omics 3(2): 35-39.
[8] Arrigoni-Blank, M. D. F., Tavares, F. F., Blank, A. F., Santos, M. C. D., Menezes, T. S. A. and Santana, A. D. D. D. (2014) In vitro conservation of sweet potato genotypes. The Scientific World Journal 2014:1-7
[9] Bekheet, S., Matter, M. & El-Ashry, A. (2016). In vitro Conservation of Jojoba (Simmondsia chinensis) Shootlet Cultures Using Osmotic Stress and Low Temperature. Middle East Journal 5(4): 396-402.
[10] Rafique, T., Yamamoto, S.-i., Fukui, K., Tanaka, D., Arizaga, M. V., Abbas, M., Matsumoto, T. & Niino, T. (2016). Cryopreservation of shoot-tips from different sugarcane varieties using D cryo-plate technique. Pakistan Journal of Agricultural Sciences 53(1): 151-158.
[11] Capuana, M. and Ponti, F. (2008) In vitro medium-term conservation of Myrtus communis L. Propagation of Ornamental Plants 8(2): 111-113.
[12] Gonçalves, S. and Romano, A. (2007) In vitro minimum growth for conservation of Drosophyllum lusitanicum. Biologia Plantarum 51(4): 795-798.
[13] Hegde, V., Makeshkumar, T., Sheela, M., Chandra, C. V., Koundinya, A., Anil, S. R., Muthuraj, R. and Darshan, S. (2017) Production of Synthetic Seed in Cassava (Manihot esculenta Crantz). Journal of Root Crops 42(2): 5-9.
[14] Ghanbarali, S., Abdollahi, M. R., Zolnorian, H., Moosavi, S. S. & Seguí-Simarro, J. M. (2016) Optimization of the conditions for production of synthetic seeds by encapsulation of axillary buds derived from minituber sprouts in potato (Solanum tuberosum). Plant Cell, Tissue and Organ Culture 126(3): 449-458.
[15] Benson, E., Harding, K., Ryan, M., Petrenko, A., Petrenko, Y. and Fuller, B. (2018) Alginate encapsulation to enhance biopreservation scope and success: a multidisciplinary review of current ideas and applications in cryopreservation and non-freezing storage. CryoLetters 39(1): 14-38.
[16] Germana, M. A., Micheli, M., Chiancone, B., Macaluso, L. and Standardi, A. (2011) Organogenesis and encapsulation of in vitro-derived propagules of Carrizo citrange [Citrus sinensis (L.) Osb. × Poncirius trifoliata (L.) Raf]. Plant Cell, Tissue and Organ Culture 106(2): 299-307.
[17] Gantait, S., Kundu, S., Ali, N. and Sahu, N. C. (2015) Synthetic seed production of medicinal plants: a review on influence of explants, encapsulation agent and matrix. Acta physiologiae plantarum 37(5): 98.
[18] Mubbarakh, S. A., Izhar, N. A., Rajasegar, A. and Subramaniam, S. (2014) Establishment of encapsulation-dehydration technique for in vitro fragmented explants of Rosa hybrida L. cv. Helmut Schmidt. Emirates Journal of Food and Agriculture 26(6): 565-576.
[19] Verma, S., Khosla, S., Choudhary, D. K. and Lal, A. K. M. (2016) Plant regeneration of A. Lakoocha from encapsulated nodal explants. Eurpean Journal of Botany, Plant Sciences and Phytology 2(3): 17-26.
[20] Gallard, A., Panis, B., Dorion, N., Swennen, R. and Grapin, A. (2008) Cryopreservation of Pelargonium apices by droplet-vitrification. CryoLetters 29(3): 243-251.
[21] Engelmann, F. (2011) Use of biotechnologies for the conservation of plant biodiversity. In Vitro Cellular & Developmental Biology-Plant 47(1): 5-16.
[22] Saha, S., Sengupta, C. and Ghosh, P. (2015) Encapsulation, short-term storage, conservation and molecular analysis to assess genetic stability in alginate-encapsulated microshoots of Ocimum kilimandscharicum Guerke. Plant Cell, Tissue and Organ Culture 120(2): 519-530.
[23] Shibli, R. A., Hawmdeh, F. A., Duwayri, M., Hadidi, N., Al-Qudah, T. S., Tahtamouni, R. W., Younes, L. S. and Zateemeh, A. (2016) Experimenting Two Cryopreservation Techniques (Vitrification and Encapsulation-Dehydration) as Approaches for Long-term Conservation of in vitro Grown Shoot Tips of Wild Fennel. Jordan Journal of Biological Sciences 9(3): 147-154.
[24] Clavero-Ramirez, I., Galvez-Farfan, J., Lopez-Aranda, J. and Gonzalez-Benito, M. (2005) Apex cryopreservation of several strawberry genotypes by two encapsulation-dehydration methods. CryoLetters 26(1): 17-24. Engelmann, F. (2011) Use of biotechnologies for the conservation of plant biodiversity. In Vitro Cellular & Developmental Biology-Plant 47(1): 5-16.
[25] Dumet, D., Korie, S. and Adeyemi, A. (2009) Cryobanking cassava germplasm at IITA. In International Symposium on Cryopreservation in Horticultural Species 908, 439-446.
Author Information
  • Department of Genetic and Biotechnology, Faculty of Science and Technology, University of Abomey-Calavi, Central Laboratory of Plant Biotechnology and Plant Breeding, Abomey-Calavi, Benin

  • Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, Laboratory of Research in Applied Biology, Abomey-Calavi, Benin

  • Department of Genetic and Biotechnology, Faculty of Science and Technology, University of Abomey-Calavi, Central Laboratory of Plant Biotechnology and Plant Breeding, Abomey-Calavi, Benin

  • Department of Genetic and Biotechnology, Faculty of Science and Technology, University of Abomey-Calavi, Central Laboratory of Plant Biotechnology and Plant Breeding, Abomey-Calavi, Benin

  • Department of Genetic and Biotechnology, Faculty of Science and Technology, University of Abomey-Calavi, Central Laboratory of Plant Biotechnology and Plant Breeding, Abomey-Calavi, Benin

Cite This Article
  • APA Style

    Arsène Mahoutondji Doussoh, Justine Sossou Dangou, Arnaud Agbidinoukoun, Serge Sètondji Houedjissin, Corneille Ahanhanzo. (2018). The Use of Encapsulation-Dehydration Technique for Short-Term Preservation of Endangered Sweet Potato Landraces (Ipomoea batatas Lam) from Benin. Journal of Plant Sciences, 6(3), 93-100. https://doi.org/10.11648/j.jps.20180603.13

    Copy | Download

    ACS Style

    Arsène Mahoutondji Doussoh; Justine Sossou Dangou; Arnaud Agbidinoukoun; Serge Sètondji Houedjissin; Corneille Ahanhanzo. The Use of Encapsulation-Dehydration Technique for Short-Term Preservation of Endangered Sweet Potato Landraces (Ipomoea batatas Lam) from Benin. J. Plant Sci. 2018, 6(3), 93-100. doi: 10.11648/j.jps.20180603.13

    Copy | Download

    AMA Style

    Arsène Mahoutondji Doussoh, Justine Sossou Dangou, Arnaud Agbidinoukoun, Serge Sètondji Houedjissin, Corneille Ahanhanzo. The Use of Encapsulation-Dehydration Technique for Short-Term Preservation of Endangered Sweet Potato Landraces (Ipomoea batatas Lam) from Benin. J Plant Sci. 2018;6(3):93-100. doi: 10.11648/j.jps.20180603.13

    Copy | Download

  • @article{10.11648/j.jps.20180603.13,
      author = {Arsène Mahoutondji Doussoh and Justine Sossou Dangou and Arnaud Agbidinoukoun and Serge Sètondji Houedjissin and Corneille Ahanhanzo},
      title = {The Use of Encapsulation-Dehydration Technique for Short-Term Preservation of Endangered Sweet Potato Landraces (Ipomoea batatas Lam) from Benin},
      journal = {Journal of Plant Sciences},
      volume = {6},
      number = {3},
      pages = {93-100},
      doi = {10.11648/j.jps.20180603.13},
      url = {https://doi.org/10.11648/j.jps.20180603.13},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.jps.20180603.13},
      abstract = {Ex situ conservation of genetic resources remains a challenge for preservation of vegetatively propagated species such as sweet potato. The objective of this study was to establish the protocol based on the encapsulation-dehydration technique for short term preservation of endangered sweet potato landraces produced in Benin. Thus, the evaporative dehydration duration on silicagel was previously determinated on the empty beads which were made using alginate sodium (3%) and calcium chloride (1.32M). Then, the young shoots of two sweet potato landraces (Koïdokpon and Dokoui carotte) growing in the screen house were cutted aseptically and desinfected with 10% sodium hypochlorite. The apices were excised on stereoscope and were encapsulated prior their dehydration on silicagel during 5 and 6h. The encapsulated apices were finally conserved in eppendorf tubes at 2°C in batches for 15 days and 90 days. The encapsulated apices were cultured in MS medium supplemented with 0.15 mg/l BAP, 0.2 mg/l NAA, 0.08 mg/l GA3 and 80 mg/l adenine sulfate. The survival and the regeneration rates were then evaluated. At our experimentation condition, the dehydration duration which allowed around 20% water content of the beads was 6h. For the encapsulated apices, the highest survival rates (59.26% and 37.04%) and the highest regeneration rate (37.04% and 11.11%) were recorded respectivelly with the landraces ''Koïdokpon'' and ''Dokoui carotte'' when the apices were dehydrated by 6h and stored for 15 days. The regeneration rates decreased according to the stored duration. Significant difference was noted on the regeneration of apices for the landraces tested. This method can be used to preserve the endangered sweet potato landraces and other species during at least three months without subcultures. It also reduce the cost of conservation in terms of consumables and permit better genotype stability during the storage.},
     year = {2018}
    }
    

    Copy | Download

  • TY  - JOUR
    T1  - The Use of Encapsulation-Dehydration Technique for Short-Term Preservation of Endangered Sweet Potato Landraces (Ipomoea batatas Lam) from Benin
    AU  - Arsène Mahoutondji Doussoh
    AU  - Justine Sossou Dangou
    AU  - Arnaud Agbidinoukoun
    AU  - Serge Sètondji Houedjissin
    AU  - Corneille Ahanhanzo
    Y1  - 2018/08/06
    PY  - 2018
    N1  - https://doi.org/10.11648/j.jps.20180603.13
    DO  - 10.11648/j.jps.20180603.13
    T2  - Journal of Plant Sciences
    JF  - Journal of Plant Sciences
    JO  - Journal of Plant Sciences
    SP  - 93
    EP  - 100
    PB  - Science Publishing Group
    SN  - 2331-0731
    UR  - https://doi.org/10.11648/j.jps.20180603.13
    AB  - Ex situ conservation of genetic resources remains a challenge for preservation of vegetatively propagated species such as sweet potato. The objective of this study was to establish the protocol based on the encapsulation-dehydration technique for short term preservation of endangered sweet potato landraces produced in Benin. Thus, the evaporative dehydration duration on silicagel was previously determinated on the empty beads which were made using alginate sodium (3%) and calcium chloride (1.32M). Then, the young shoots of two sweet potato landraces (Koïdokpon and Dokoui carotte) growing in the screen house were cutted aseptically and desinfected with 10% sodium hypochlorite. The apices were excised on stereoscope and were encapsulated prior their dehydration on silicagel during 5 and 6h. The encapsulated apices were finally conserved in eppendorf tubes at 2°C in batches for 15 days and 90 days. The encapsulated apices were cultured in MS medium supplemented with 0.15 mg/l BAP, 0.2 mg/l NAA, 0.08 mg/l GA3 and 80 mg/l adenine sulfate. The survival and the regeneration rates were then evaluated. At our experimentation condition, the dehydration duration which allowed around 20% water content of the beads was 6h. For the encapsulated apices, the highest survival rates (59.26% and 37.04%) and the highest regeneration rate (37.04% and 11.11%) were recorded respectivelly with the landraces ''Koïdokpon'' and ''Dokoui carotte'' when the apices were dehydrated by 6h and stored for 15 days. The regeneration rates decreased according to the stored duration. Significant difference was noted on the regeneration of apices for the landraces tested. This method can be used to preserve the endangered sweet potato landraces and other species during at least three months without subcultures. It also reduce the cost of conservation in terms of consumables and permit better genotype stability during the storage.
    VL  - 6
    IS  - 3
    ER  - 

    Copy | Download

  • Sections