Medium Improvement for Higher Growth and Longer Stationary Phase of Dunaliella

Suong Nguyen; Duc Tran; Sixto Portilla; Trung Vo

doi:doi:10.11648/j.jps.20140201.13

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Medium Improvement for Higher Growth and Longer Stationary Phase of Dunaliella

Suong Nguyen, Duc Tran, Sixto Portilla, Trung Vo

Published in Journal of Plant Sciences (Volume 2, Issue 1)

Received: 31 December 2013 Accepted: Published: 20 January 2014

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Abstract

Beta-carotene is a valuable carotenoid in high demand as a natural food coloring agent, provitamin A, additive to cosmetics, and health food. It can be accumulated up to more than 10% of cellular dry weight of Dunaliella salina under carotenogenic conditions such as high irradiance, high temperature, high salt concentration and nutrient deficiency. High beta-carotene productivity in Dunaliella is best achieved in a two-phase culture system through biomass optimization and beta-carotene induction. A low-cost enriched natural seawater medium (MD4) was previously investigated for biomass optimization (Tran et al., 2014); However, the culture declined rapidly after reaching the stationary phase. Thus the present study is to further improve the effectiveness of this enriched natural seawater medium (MD4) for higher growth and longer stationary phase in order to avoid quick crash phase. Algal culture in MD4 medium used as control medium was subjected to 13 different feeding treatments (TM1  TM13) using a matrix of concentrations of various compounds (NPK, KNO3, KH2PO4). Dunaliella growth was determined based on chlorophyll concentration, cell density. Results revealed the best feeding treatment was TM4 (NPK 0.15g/l) with cell density doubling one week compared with cell density in the control medium, and is recommended for use in the first phase biomass optimization of Dunaliella.

Published in	Journal of Plant Sciences (Volume 2, Issue 1)
DOI	10.11648/j.jps.20140201.13
Page(s)	9-13
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Algae, Carotene, Chlorophyll, Cultivation, Dunaliella, Medium

References

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APA Style

Suong Nguyen, Duc Tran, Sixto Portilla, Trung Vo. (2014). Medium Improvement for Higher Growth and Longer Stationary Phase of Dunaliella. Journal of Plant Sciences, 2(1), 9-13. https://doi.org/10.11648/j.jps.20140201.13

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ACS Style

Suong Nguyen; Duc Tran; Sixto Portilla; Trung Vo. Medium Improvement for Higher Growth and Longer Stationary Phase of Dunaliella. J. Plant Sci. 2014, 2(1), 9-13. doi: 10.11648/j.jps.20140201.13

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AMA Style

Suong Nguyen, Duc Tran, Sixto Portilla, Trung Vo. Medium Improvement for Higher Growth and Longer Stationary Phase of Dunaliella. J Plant Sci. 2014;2(1):9-13. doi: 10.11648/j.jps.20140201.13

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@article{10.11648/j.jps.20140201.13,
  author = {Suong Nguyen and Duc Tran and Sixto Portilla and Trung Vo},
  title = {Medium Improvement for Higher Growth and Longer Stationary Phase of Dunaliella},
  journal = {Journal of Plant Sciences},
  volume = {2},
  number = {1},
  pages = {9-13},
  doi = {10.11648/j.jps.20140201.13},
  url = {https://doi.org/10.11648/j.jps.20140201.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jps.20140201.13},
  abstract = {Beta-carotene is a valuable carotenoid in high demand as a natural food coloring agent, provitamin A, additive to cosmetics, and health food.  It can be accumulated up to more than 10% of cellular dry weight of Dunaliella salina under carotenogenic conditions such as high irradiance, high temperature, high salt concentration and nutrient deficiency. High beta-carotene productivity in Dunaliella is best achieved in a two-phase culture system through biomass optimization and beta-carotene induction. A low-cost enriched natural seawater medium (MD4) was previously investigated for biomass optimization (Tran et al., 2014); However, the culture declined rapidly after reaching the stationary phase.  Thus the present study is to further improve the effectiveness of this enriched natural seawater medium (MD4) for higher growth and longer stationary phase in order to avoid quick crash phase. Algal culture in MD4 medium used as control medium was subjected to 13 different feeding treatments (TM1  TM13) using a matrix of concentrations of various compounds (NPK, KNO3, KH2PO4). Dunaliella growth was determined based on chlorophyll concentration, cell density.  Results revealed the best feeding treatment was TM4 (NPK 0.15g/l) with cell density doubling one week compared with cell density in the control medium, and is recommended for use in the first phase biomass optimization of Dunaliella.},
 year = {2014}
}

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TY  - JOUR
T1  - Medium Improvement for Higher Growth and Longer Stationary Phase of Dunaliella
AU  - Suong Nguyen
AU  - Duc Tran
AU  - Sixto Portilla
AU  - Trung Vo
Y1  - 2014/01/20
PY  - 2014
N1  - https://doi.org/10.11648/j.jps.20140201.13
DO  - 10.11648/j.jps.20140201.13
T2  - Journal of Plant Sciences
JF  - Journal of Plant Sciences
JO  - Journal of Plant Sciences
SP  - 9
EP  - 13
PB  - Science Publishing Group
SN  - 2331-0731
UR  - https://doi.org/10.11648/j.jps.20140201.13
AB  - Beta-carotene is a valuable carotenoid in high demand as a natural food coloring agent, provitamin A, additive to cosmetics, and health food.  It can be accumulated up to more than 10% of cellular dry weight of Dunaliella salina under carotenogenic conditions such as high irradiance, high temperature, high salt concentration and nutrient deficiency. High beta-carotene productivity in Dunaliella is best achieved in a two-phase culture system through biomass optimization and beta-carotene induction. A low-cost enriched natural seawater medium (MD4) was previously investigated for biomass optimization (Tran et al., 2014); However, the culture declined rapidly after reaching the stationary phase.  Thus the present study is to further improve the effectiveness of this enriched natural seawater medium (MD4) for higher growth and longer stationary phase in order to avoid quick crash phase. Algal culture in MD4 medium used as control medium was subjected to 13 different feeding treatments (TM1  TM13) using a matrix of concentrations of various compounds (NPK, KNO3, KH2PO4). Dunaliella growth was determined based on chlorophyll concentration, cell density.  Results revealed the best feeding treatment was TM4 (NPK 0.15g/l) with cell density doubling one week compared with cell density in the control medium, and is recommended for use in the first phase biomass optimization of Dunaliella.
VL  - 2
IS  - 1
ER  -

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Author Information

Suong Nguyen

School of Biotechnology, International University-VNU, Vietnam
Duc Tran

School of Biotechnology, International University-VNU, Vietnam
Sixto Portilla

Center for Environmental Research and Coastal Oceans Monitoring, Molloy College, 100 Hempstead Avenue, Rockville Centre, NY
Trung Vo

School of Biotechnology, International University-VNU, Vietnam

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APA Style

Suong Nguyen, Duc Tran, Sixto Portilla, Trung Vo. (2014). Medium Improvement for Higher Growth and Longer Stationary Phase of Dunaliella. Journal of Plant Sciences, 2(1), 9-13. https://doi.org/10.11648/j.jps.20140201.13

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ACS Style

Suong Nguyen; Duc Tran; Sixto Portilla; Trung Vo. Medium Improvement for Higher Growth and Longer Stationary Phase of Dunaliella. J. Plant Sci. 2014, 2(1), 9-13. doi: 10.11648/j.jps.20140201.13

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AMA Style

Suong Nguyen, Duc Tran, Sixto Portilla, Trung Vo. Medium Improvement for Higher Growth and Longer Stationary Phase of Dunaliella. J Plant Sci. 2014;2(1):9-13. doi: 10.11648/j.jps.20140201.13

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@article{10.11648/j.jps.20140201.13,
  author = {Suong Nguyen and Duc Tran and Sixto Portilla and Trung Vo},
  title = {Medium Improvement for Higher Growth and Longer Stationary Phase of Dunaliella},
  journal = {Journal of Plant Sciences},
  volume = {2},
  number = {1},
  pages = {9-13},
  doi = {10.11648/j.jps.20140201.13},
  url = {https://doi.org/10.11648/j.jps.20140201.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jps.20140201.13},
  abstract = {Beta-carotene is a valuable carotenoid in high demand as a natural food coloring agent, provitamin A, additive to cosmetics, and health food.  It can be accumulated up to more than 10% of cellular dry weight of Dunaliella salina under carotenogenic conditions such as high irradiance, high temperature, high salt concentration and nutrient deficiency. High beta-carotene productivity in Dunaliella is best achieved in a two-phase culture system through biomass optimization and beta-carotene induction. A low-cost enriched natural seawater medium (MD4) was previously investigated for biomass optimization (Tran et al., 2014); However, the culture declined rapidly after reaching the stationary phase.  Thus the present study is to further improve the effectiveness of this enriched natural seawater medium (MD4) for higher growth and longer stationary phase in order to avoid quick crash phase. Algal culture in MD4 medium used as control medium was subjected to 13 different feeding treatments (TM1  TM13) using a matrix of concentrations of various compounds (NPK, KNO3, KH2PO4). Dunaliella growth was determined based on chlorophyll concentration, cell density.  Results revealed the best feeding treatment was TM4 (NPK 0.15g/l) with cell density doubling one week compared with cell density in the control medium, and is recommended for use in the first phase biomass optimization of Dunaliella.},
 year = {2014}
}

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TY  - JOUR
T1  - Medium Improvement for Higher Growth and Longer Stationary Phase of Dunaliella
AU  - Suong Nguyen
AU  - Duc Tran
AU  - Sixto Portilla
AU  - Trung Vo
Y1  - 2014/01/20
PY  - 2014
N1  - https://doi.org/10.11648/j.jps.20140201.13
DO  - 10.11648/j.jps.20140201.13
T2  - Journal of Plant Sciences
JF  - Journal of Plant Sciences
JO  - Journal of Plant Sciences
SP  - 9
EP  - 13
PB  - Science Publishing Group
SN  - 2331-0731
UR  - https://doi.org/10.11648/j.jps.20140201.13
AB  - Beta-carotene is a valuable carotenoid in high demand as a natural food coloring agent, provitamin A, additive to cosmetics, and health food.  It can be accumulated up to more than 10% of cellular dry weight of Dunaliella salina under carotenogenic conditions such as high irradiance, high temperature, high salt concentration and nutrient deficiency. High beta-carotene productivity in Dunaliella is best achieved in a two-phase culture system through biomass optimization and beta-carotene induction. A low-cost enriched natural seawater medium (MD4) was previously investigated for biomass optimization (Tran et al., 2014); However, the culture declined rapidly after reaching the stationary phase.  Thus the present study is to further improve the effectiveness of this enriched natural seawater medium (MD4) for higher growth and longer stationary phase in order to avoid quick crash phase. Algal culture in MD4 medium used as control medium was subjected to 13 different feeding treatments (TM1  TM13) using a matrix of concentrations of various compounds (NPK, KNO3, KH2PO4). Dunaliella growth was determined based on chlorophyll concentration, cell density.  Results revealed the best feeding treatment was TM4 (NPK 0.15g/l) with cell density doubling one week compared with cell density in the control medium, and is recommended for use in the first phase biomass optimization of Dunaliella.
VL  - 2
IS  - 1
ER  -

Copy | Download