LC-MS and NMR Based Structural Characterization and Isotopic Abundance Ratio Analysis of Magnesium Gluconate Treated with the Consciousness Energy Healing
Advances in Bioscience and Bioengineering
Volume 5, Issue 2, April 2017, Pages: 22-31
Received: Feb. 24, 2017; Accepted: Mar. 8, 2017; Published: Apr. 1, 2017
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Authors
Mahendra Kumar Trivedi, Trivedi Global, Inc., Henderson, Nevada, USA
Alice Branton, Trivedi Global, Inc., Henderson, Nevada, USA
Dahryn Trivedi, Trivedi Global, Inc., Henderson, Nevada, USA
Gopal Nayak, Trivedi Global, Inc., Henderson, Nevada, USA
William Dean Plikerd, Trivedi Global, Inc., Henderson, Nevada, USA
Peter L. Surguy, Trivedi Global, Inc., Henderson, Nevada, USA
Robert John Kock, Trivedi Global, Inc., Henderson, Nevada, USA
Rolando Baptista Piedad, Trivedi Global, Inc., Henderson, Nevada, USA
Russell Phillip Callas, Trivedi Global, Inc., Henderson, Nevada, USA
Sakina A. Ansari, Trivedi Global, Inc., Henderson, Nevada, USA
Sandra Lee Barrett, Trivedi Global, Inc., Henderson, Nevada, USA
Sara Friedman, Trivedi Global, Inc., Henderson, Nevada, USA
Steven Lee Christie, Trivedi Global, Inc., Henderson, Nevada, USA
Su-Mei Chen Liu, Trivedi Global, Inc., Henderson, Nevada, USA
Susan Elizabeth Starling, Trivedi Global, Inc., Henderson, Nevada, USA
Susan Jones, Trivedi Global, Inc., Henderson, Nevada, USA
Susan Mardis Allen, Trivedi Global, Inc., Henderson, Nevada, USA
Susanne Kathrin Wasmus, Trivedi Global, Inc., Henderson, Nevada, USA
Terry Ann Benczik, Trivedi Global, Inc., Henderson, Nevada, USA
Thomas Charles Slade, Trivedi Global, Inc., Henderson, Nevada, USA
Thomas Orban, Trivedi Global, Inc., Henderson, Nevada, USA
Victoria L. Vannes, Trivedi Global, Inc., Henderson, Nevada, USA
Victoria Margot Schlosser, Trivedi Global, Inc., Henderson, Nevada, USA
Yusif Sarkis Yamin Albino, Trivedi Global, Inc., Henderson, Nevada, USA
Parthasarathi Panda, Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, Madhya Pradesh, India
Kalyan Kumar Sethi, Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, Madhya Pradesh, India
Snehasis Jana, Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, Madhya Pradesh, India
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Abstract
Magnesium gluconate is widely used pharmaceutical/nutraceutical compound for the prevention and treatment of magnesium deficiency diseases. The present study was designed to explore the effect of The Trivedi Effect® - Energy of Consciousness Healing Treatment (Biofield Energy Healing Treatment) on magnesium gluconate for the change in the structural properties and isotopic abundance ratio (PM+1/PM and PM+2/PM) using LC-MS and NMR spectroscopy. Magnesium gluconate was divided into two parts – one part was control, and another part was treated with The Trivedi Effect® - Energy of Consciousness Healing Treatment remotely by twenty renowned Biofield Energy Healers and defined as The Trivedi Effect® treated sample. The LC-MS analysis of both the control and Biofield Energy Treated samples indicated the presence of mass of the protonated magnesium gluconate at m/z 415 at the retention time of 1.52 min and fragmentation pattern of both samples were almost identical. The relative peak intensities of the fragment ions were significantly altered in the treated sample compared to the control sample. The proton and carbon signals for CH, CH2 and CO groups in the proton and carbon NMR spectra of the control and treated samples were found same. The percentage change in the isotopic abundance ratio of PM+1/PM (2H/1H or 13C/12C or 17O/16O or 25Mg/24Mg) was significantly decreased in the treated sample by 48.87% compared to the control sample. Subsequently, the isotopic abundance ratio of PM+2/PM (18O/16O or 26Mg/24Mg) in the treated sample was significantly increased by 29.18% compared with the control sample. In summary, 13C, 2H, 17O, and 25Mg contributions from (C12H23MgO14)+ to m/z 416; 18O and 26Mg contributions from (C12H23MgO14)+ to m/z 417 in the treated sample were significantly altered compared with the control sample. Thus, The Trivedi Effect® Treated magnesium gluconate might be helpful to design the novel potent enzyme inhibitors using its kinetic isotope effects. Consequently, The Trivedi Effect® Treated magnesium gluconate would be valuable for designing better pharmaceutical and/or nutraceutical formulations through its altered physicochemical and thermal properties, which might be providing better therapeutic response against various diseases such as diabetes mellitus, allergy, aging, inflammatory diseases, immunological disorders, and other chronic infections.
Keywords
Magnesium Gluconate, The Trivedi Effect®, Consciousness Energy Healing Treatment, Biofield Energy Healing Treatment, Isotope Effects, Biofield Energy Healers, LC-MS, Isotopic Abundance
To cite this article
Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, William Dean Plikerd, Peter L. Surguy, Robert John Kock, Rolando Baptista Piedad, Russell Phillip Callas, Sakina A. Ansari, Sandra Lee Barrett, Sara Friedman, Steven Lee Christie, Su-Mei Chen Liu, Susan Elizabeth Starling, Susan Jones, Susan Mardis Allen, Susanne Kathrin Wasmus, Terry Ann Benczik, Thomas Charles Slade, Thomas Orban, Victoria L. Vannes, Victoria Margot Schlosser, Yusif Sarkis Yamin Albino, Parthasarathi Panda, Kalyan Kumar Sethi, Snehasis Jana, LC-MS and NMR Based Structural Characterization and Isotopic Abundance Ratio Analysis of Magnesium Gluconate Treated with the Consciousness Energy Healing, Advances in Bioscience and Bioengineering. Vol. 5, No. 2, 2017, pp. 22-31. doi: 10.11648/j.abb.20170502.11
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Copyright © 2017 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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